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Characterization of metabolic changes associated with the functional development of 3D engineered tissues by non-invasive, dynamic measurement of individual cell redox ratios.

Abstract：

:Non-invasive approaches to assess tissue function could improve significantly current methods to diagnose diseases and optimize engineered tissues. In this study, we describe a two-photon excited fluorescence microscopy approach that relies entirely on endogenous fluorophores to dynamically quantify functional metabolic readouts from individual cells within three-dimensional engineered tissues undergoing adipogenic differentiation over six months. Specifically, we employ an automated approach to analyze 3D image volumes and extract a redox ratio of metabolic cofactors. We identify a decrease in redox ratio over the first two months of culture that is associated with stem cell differentiation and lipogenesis. In addition, we demonstrate that the presence of endothelial cells facilitate greater cell numbers deeper within the engineered tissues. Since traditional assessments of engineered tissue structure and function are destructive and logistically intensive, this non-destructive, label-free approach offers a potentially powerful high-content characterization tool for optimizing tissue engineering protocols and assessing engineered tissue implants.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Quinn KP,Bellas E,Fourligas N,Lee K,Kaplan DL,Georgakoudi I

doi

10.1016/j.biomaterials.2012.04.024

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

5341-8

issue

21

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(12)00446-2

journal_volume

33

pub_type

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