Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma.

Abstract:

AIM:To clarify the association of glypican-3 (GPC3) expression with Wnt and other growth signaling molecules in hepatocellular carcinoma (HCC). METHODS:Expression of GPC3, Wnt, matrix metalloproteinases (MMPs), sulfatase (SULF)1, SULF2, and other growth signaling molecules was analyzed in HCC cell lines and tissue samples by real-time reverse transcription-polymerase chain reaction, immunoblotting, and/or immunostaining. Expression of various genes in GPC3 siRNA-transfected HCC cells was analyzed. RESULTS:GPC3 was overexpressed in most HCCs at mRNA and protein levels and its serum levels were significantly higher in patients with HCC than in non-HCC subjects (P < 0.05). Altered expressions of various MMPs and growth signaling molecules, some of which were correlated with GPC3 expression, were observed in HCCs. Down-regulation of GPC3 expression by siRNA in GPC3-overexpressing HCC cell lines resulted in a significant decrease in expressions of MMP2, MMP14, fibroblast growth factor receptor 1, insulin-like growth factor 1 receptor. GPC3 expression was significantly correlated with nuclear/cytoplasmic localization of beta-catenin. CONCLUSION:These results suggest that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC.

journal_name

World J Gastroenterol

authors

Akutsu N,Yamamoto H,Sasaki S,Taniguchi H,Arimura Y,Imai K,Shinomura Y

doi

10.3748/wjg.v16.i28.3521

subject

Has Abstract

pub_date

2010-07-28 00:00:00

pages

3521-8

issue

28

eissn

1007-9327

issn

2219-2840

journal_volume

16

pub_type

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