Abstract:
AIM:To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS:The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS:Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4+/-3.12%) compared to the control group (3.12+/-0.13%, P < 0.01). CONCLUSION:Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Zhu JS,Wang L,Cheng GQ,Li Q,Zhu ZM,Zhu Ldoi
10.3748/wjg.v11.i47.7536keywords:
subject
Has Abstractpub_date
2005-12-21 00:00:00pages
7536-40issue
47eissn
1007-9327issn
2219-2840journal_volume
11pub_type
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