Abstract:
BACKGROUND:Vascular endothelial growth factor (VEGF) is a therapeutic target in gastrointestinal cancer (GiC). However, its in vivo visualisation could not be achieved to date with endoscopic techniques. Confocal laser endomicroscopy (CLE) is a novel imaging technique for gastrointestinal endoscopy providing in vivo microscopy at subcellular resolution. The aim of the study was to evaluate CLE for in vivo molecular imaging of VEGF in GiC. METHODS:Molecular imaging of tumours in APCmin mice, in xenograft models and in surgical specimens of patients with colorectal cancer (CRC) was achieved after application of labelled antibodies. The tumour sites were scanned with the probe for the strongest specific fluorescent signal. From all tumour sites examined with CLE in vivo, targeted specimens were obtained for histology, immunohistochemistry (IHC) and fluorescence microscopy. RESULTS:A VEGF-specific signal was visualised in vivo in 13/15 APCmin mice and in 9/10 xenograft tumours. CLE enabled the cytoplasmatic distribution of VEGF to be displayed due to its subcellular resolution. In human tissue, a VEGF-specific signal was observed in 12/13 malignant specimens and in 10/11 samples from healthy mucosa from the patients (p<0.03). CLE findings correlated well with ex vivo microscopy. CONCLUSION:In vivo molecular imaging with specific targeting of VEGF is possible in murine tumours, human xenografts and tissue specimens using CLE. CLE with similar probes can be performed in human colonoscopy. Therefore-from a technical point of view-in vivo molecular imaging is transferable to stratification of patients with CRC during endoscopy even today. CLE could contribute to the identification of lesions at risk and potentially predict response to targeted treatment.
journal_name
Gutjournal_title
Gutauthors
Foersch S,Kiesslich R,Waldner MJ,Delaney P,Galle PR,Neurath MF,Goetz Mdoi
10.1136/gut.2009.202986subject
Has Abstractpub_date
2010-08-01 00:00:00pages
1046-55issue
8eissn
0017-5749issn
1468-3288pii
59/8/1046journal_volume
59pub_type
杂志文章相关文献
GUT文献大全abstract::The use of tripotassium dicitrato bismuthate (TDB) has been suggested recently for cimetidine resistant duodenal ulcers. This study compares the efficacy of TDB with two different cimetidine dosages in the treatment of duodenal ulcer patients who failed to respond to an eight week therapy with H2-blockers. Fifty two p...
journal_title:Gut
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/gut.28.7.907
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:Escherichia coli is part of the normal gastrointestinal microflora which exerts a barrier effect against enteropathogens. Several E coli strains develop a protective effect against other Enterobacteriaceae. AIMS:Two E coli strains, EM0, a human faecal strain, and JM105 K-12 were tested for their ability to ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.49.1.47
更新日期:2001-07-01 00:00:00
abstract::Clinical history, questionnaire data and response to antisecretory therapy are insufficient to make a conclusive diagnosis of GERD in isolation, but are of value in determining need for further investigation. Conclusive evidence for reflux on oesophageal testing include advanced grade erosive oesophagitis (LA grades C...
journal_title:Gut
pub_type: 杂志文章,评审
doi:10.1136/gutjnl-2017-314722
更新日期:2018-07-01 00:00:00
abstract::The metabolism of tauroursodeoxycholic acid orally administered and its effects on the bile acid pool of patients with asymptomatic/mildly symptomatic primary biliary cirrhosis is described. Patients were randomly assigned 500, 1000, or 1500 mg/day of tauroursodeoxycholate for six months. Biliary and serum bile acids ...
journal_title:Gut
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/gut.38.3.439
更新日期:1996-03-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate whether patients with increased or decreased gastric motility can be differentiated from healthy volunteers by means of real time magnetic resonance imaging (MRI). PATIENTS AND METHODS:Ten healthy volunteers, 10 patients with gastroparesis, and 10 patients with function...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2003.038588
更新日期:2004-09-01 00:00:00
abstract:BACKGROUND:In hereditary pancreatitis mutations of exons 2 (N21I) and 3 (R117H) of the cationic trypsinogen gene have been described. AIMS:To investigate whether the same mutations can also be found in patients with chronic alcoholic pancreatitis. METHODS:Leucocyte DNA was prepared from 23 patients with chronic alcoh...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.44.3.413
更新日期:1999-03-01 00:00:00
abstract:BACKGROUND:Cyclooxygenase 2 (COX-2) and matrix metalloproteinases (MMPs) have been implicated in tissue injury and fibrogenesis in animal models but little is known regarding their role in hepatitis C virus (HCV) related liver disease in humans. AIMS:To characterise the intrahepatic expression pattern of COX-2 and MMP...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2003.038364
更新日期:2004-11-01 00:00:00
abstract::Three pH electrodes in clinical use were examined--(1) antimony electrode with remote reference electrode (Synectics 0011), (2) glass electrode with remote reference electrode (Microelectrodes Inc. MI 506) and (3) combined glass electrode with integral reference electrode (Radiometer GK2801C). In vitro studies showed ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.28.8.935
更新日期:1987-08-01 00:00:00
abstract::Macrophage major histocompatibility complex (MHC) class II antigen expression is associated with defective antigen presentation to T lymphocytes in animals and is predictive of patient outcome after major trauma or sepsis. In this study, class II antigen (HLA-DR and DQ) expression on peripheral blood monocytes was inv...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.35.4.511
更新日期:1994-04-01 00:00:00
abstract:OBJECTIVE:To examine the relationship between Mediterranean diet and risk of later-onset Crohn's disease (CD) or ulcerative colitis (UC). DESIGN:We conducted a prospective cohort study of 83 147 participants (age range: 45-79 years) enrolled in the Cohort of Swedish Men and Swedish Mammography Cohort. A validated food...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2019-319505
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Lamina propria (LPLs) and intraepithelial (IELs) lymphocytes are markedly increased in coeliac mucosa, and are thought to play a crucial role in the generation of villous atrophy in coeliac disease (CD). However, the mechanisms by which they mediate the killing of enterocytes in this condition are still poor...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.49.3.380
更新日期:2001-09-01 00:00:00
abstract::During the clinical investigation of patients with gastrointestinal disease by exhaled breath hydrogen measurement, the occurrence of inexplicable variations in recorded hydrogen values led to a search for extra intestinal factors which were capable of adversely influencing breath hydrogen concentration and impairing ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.12.1349
更新日期:1985-12-01 00:00:00
abstract:BACKGROUND:Previous experimental studies have suggested many possible anti-cancer mechanisms for green tea, but epidemiological evidence for the effect of green tea consumption on gastric cancer risk is conflicting. OBJECTIVE:To examine the association between green tea consumption and gastric cancer. METHODS:We anal...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2008.166710
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:Endoscopic diagnosis of short segments of Barrett's epithelium (SSBE)' is difficult and its meaning in terms of the presence of specialised columnar epithelium (SCE) has not been prospectively evaluated. AIMS:To evaluate the prevalence of SCE in patients with an endoscopic diagnosis of SSBE and in individua...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.42.5.659
更新日期:1998-05-01 00:00:00
abstract::Large numbers of cells are shed from small intestinal mucosa but they are so rapidly destroyed that there is little direct information about their morphological types. Material obtained by washing the human and rat small intestine was immediately processed and examined by light and electron microscopy. Small intestina...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.11.3.217
更新日期:1970-03-01 00:00:00
abstract:OBJECTIVE:Serrated polyps (SPs) are an important cause of postcolonoscopy colorectal cancers (PCCRCs), which is likely the result of suboptimal SP detection during colonoscopy. We assessed the long-term effect of a simple educational intervention focusing on optimising SP detection. DESIGN:An educational intervention,...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2019-319804
更新日期:2020-12-01 00:00:00
abstract::This paper compares the mortality of 525 men admitted to U.S. Army hospitals in 1944 with ulcerative colitis compared with 518 controls matched for age, race, and rank. The excess mortality in the ulcerative colitis group as compared with the control group was due to approximately equal proportions of deaths from ulce...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.4.2.183
更新日期:1963-06-01 00:00:00
abstract::Infection with the delta agent can only occur in the context of coexistent hepatitis B virus infection. We describe a patient in whom the clinical features of acute delta hepatitis developed when seroconversion from hepatitis B surface antigen to antibody had already occurred and diagnosis of recent acute hepatitis B ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.2.212
更新日期:1985-02-01 00:00:00
abstract::One-hundred-and-one normal neonates were examined manometrically in the first day of life to assess anorectal function. In order to overcome some of the difficulties in subjective interpretation of results, the stimuli used and the response pattern seen in the anal canal were measured. In the unstimulated state, all s...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.18.12.1036
更新日期:1977-12-01 00:00:00
abstract::Ultrastructural examination of biopsies showing Helicobacter pylori associated chronic gastritis reveals close attachment between gastric surface epithelial cells and the organism. The finding of 'adhesion pedestals', which represents a cellular response to the presence of the organism, is analogous to the response of...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.2.134
更新日期:1990-02-01 00:00:00
abstract:BACKGROUND:Several inflammatory disorders of the intestine are characterised by enhanced expression of tumour necrosis factor alpha (TNF-alpha). Monocytes and macrophages have been suggested as a major cellular source of TNF-alpha in human gut, whereas mast cells, although known to be capable of producing TNF-alpha, ha...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.44.5.643
更新日期:1999-05-01 00:00:00
abstract::Perhexiline maleate is an antianginal agent which depends on hepatic oxidation for its elimination. Its use may be complicated by the development of peripheral neuropathy and liver damage. The majority of patients with perhexiline neuropathy have an impaired ability to effect metabolic drug oxidation which is genetica...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.25.10.1057
更新日期:1984-10-01 00:00:00
abstract::Seven patients with hypoalbuminaemia and extensive jejunoileal Crohn's disease were treated with an elemental diet for 28 to 56 days. Over this time there was an increase in total plasma proteins from mean of 49.1 to 59.0 g/l and a mean rise in serum albumin from 20.7 to 30.0 g/l (P less than 0.05). In addition, there...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.22.5.383
更新日期:1981-05-01 00:00:00
abstract:BACKGROUND AND AIMS:Independent component analysis (ICA) of the electroencephalogram (EEG) overcomes many of the classical problems in EEG analysis. We used ICA to determine the brain responses to painful stimulation of the oesophagus. METHODS:Twelve subjects with a median age of 41 years were included. With a nasal e...
journal_title:Gut
pub_type: 临床试验,杂志文章
doi:10.1136/gut.2005.068460
更新日期:2006-05-01 00:00:00
abstract::The colonic biopsy specimens of 22 patients with colitis and positive stool cultures for Campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate Campylobacter colitis from chronic inflammatory bowel disease. In addition we tried to identify Campylobacter incl...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.9.945
更新日期:1985-09-01 00:00:00
abstract::The host's humoral immune response to Helicobacter pylori has been used in the diagnosis of active infection with these organisms. Several commercial tests are available but there are few and unconfirmed reports of their efficacy. This study aimed to assess and compare the efficacy of the following H pylori serologica...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.34.4.461
更新日期:1993-04-01 00:00:00
abstract::Studies in the rat jejunum in vivo have shown that 5-hydroxytryptamine (5-HT) causes secretion of fluid and luminal release of prostaglandin (PG) E2. These effects can be blocked by indomethacin and ketanserin, which suggests that PGE2 may be an important intermediate in the transduction mechanism leading to 5-HT indu...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.29.10.1337
更新日期:1988-10-01 00:00:00
abstract:BACKGROUND:Insulin resistance is a frequent feature of chronic hepatitis C. Whether insulin resistance could be the cause or consequence of steatosis and fibrosis is unknown. The ability of HCV genotype 3 to promote steatosis by itself provides an unique opportunity to answer this question. AIMS:The aim of the present...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2004.050302
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND:Recent reports have described a modulating influence of nitric oxide (NO) on intestinal mucosal permeability and have implicated a role for mast cells in this NO mediated process. AIMS:To assess further the contribution of mast cells to the mucosal permeability changes elicited by the NO synthase (NOS) inhi...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.41.5.636
更新日期:1997-11-01 00:00:00
abstract:BACKGROUND:Patients with Crohn's disease suffer from intestinal bile acid malabsorption. Intestinal bile acid absorption is mediated by the apical sodium dependent bile acid transporter ASBT/IBAT (SLC10A2). In rats, ASBT is induced by glucocorticoids. AIMS:To study whether human ASBT is activated by glucocorticoids an...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.53.1.78
更新日期:2004-01-01 00:00:00