Rapid structural alterations of the active zone lead to sustained changes in neurotransmitter release.

abstract：:Seasonal variations in the properties of rabbit-liver fructose 1,6-bisphosphatase have now been linked to corresponding changes in the levels of proteolytic activity in the liver extracts. Incubation of native fructose 1,6-bisphosphatase with purified liver lysosomes causes a 3-fold increase in catalytic activity at p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pontremoli S,Melloni E,Balestrero F,Franzi AT,De Flora A,Horecker BL

更新日期：1973-02-01 00:00:00

abstract：:Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)-PCR describes a new paradigm for primer design that renders assays as efficient as symmetric...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanchez JA,Pierce KE,Rice JE,Wangh LJ

更新日期：2004-02-17 00:00:00

abstract：:Two 1,4-benzoquinone derivatives, found in the venom of the scorpion Diplocentrus melici following exposure to air, have been isolated, characterized, synthesized, and assessed for antimicrobial activities. Initially a white, viscous liquid, the extracted venom colors within minutes under ambient conditions. From this...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carcamo-Noriega EN,Sathyamoorthi S,Banerjee S,Gnanamani E,Mendoza-Trujillo M,Mata-Espinosa D,Hernández-Pando R,Veytia-Bucheli JI,Possani LD,Zare RN

更新日期：2019-06-25 00:00:00

abstract：:Optogenetic methods have been highly effective for suppressing neural activity and modulating behavior in rodents, but effects have been much smaller in primates, which have much larger brains. Here, we present a suite of technologies to use optogenetics effectively in primates and apply these tools to a classic quest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acker L,Pino EN,Boyden ES,Desimone R

更新日期：2016-11-15 00:00:00

abstract：:The proliferation of non-neoplastic T lymphocytes is regulated, in part, by the coordinated expression of genes encoding T-cell growth factor (interleukin 2, IL2), IL2 receptors (IL2R), and transferrin receptors (TFR). In addition to growth factors and their receptors, protooncogenes may regulate lymphocyte proliferat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reed JC,Alpers JD,Nowell PC,Hoover RG

更新日期：1986-06-01 00:00:00

abstract：:We have sequenced the mutant beta-actin gene of a tumorigenic human fibroblast cell line (HuT-14T) and found that it carries three mutations that alter the amino acids at positions 36, 83, and 244 as well as a 22-base-pair "insertion" sequence, in the 5' intron, not present in a wild-type gene. The less tumorigenic ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin CS,Ng SY,Gunning P,Kedes L,Leavitt J

更新日期：1985-10-01 00:00:00

abstract：:The rapid worldwide spread of severe acute respiratory syndrome demonstrated the potential threat an infectious disease poses in a closely interconnected and interdependent world. Here we introduce a probabilistic model that describes the worldwide spread of infectious diseases and demonstrate that a forecast of the g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hufnagel L,Brockmann D,Geisel T

更新日期：2004-10-19 00:00:00

abstract：:Pentamidine, diminazene aceturate (Berenil), isometamidium chloride (Samorin), and ethidium bromide, which are important antitrypanosomal drugs, promote linearization of Trypanosoma equiperdum minicircle DNA (the principal component of kinetoplast DNA, the mitochondrial DNA in these parasites). This effect occurs at t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shapiro TA,Englund PT

更新日期：1990-02-01 00:00:00

abstract：:Mice lacking the beta1-subunit (gene, Kcnmb1; protein, BK-beta1) of the large Ca-activated K channel (BK) are hypertensive. This phenotype is thought to result from diminished BK currents in vascular smooth muscle where BK-beta1 is an ancillary subunit. However, the beta1-subunit is also expressed in the renal connect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grimm PR,Irsik DL,Settles DC,Holtzclaw JD,Sansom SC

更新日期：2009-07-14 00:00:00

abstract：:Although rigorous measures of similarity for sequence and structure are now well established, the problem of defining functional relationships has been particularly daunting. Here, we present several manifold embedding techniques to compute distances between Gene Ontology (GO) functional annotations and consequently e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lerman G,Shakhnovich BE

更新日期：2007-07-03 00:00:00

abstract：:We have used restriction endonuclease mapping of cell DNA to investigate the structure of the beta-globin gene in beta-thalassemias. Among 17 individuals with beta +- and beta 0-thalassemia, we observed three patients of Indian origin with beta 0-thalassemia whose DNA revealed a consistent mapping abnormality. In one ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orkin SH,Old JM,Weatherall DJ,Nathan DG

更新日期：1979-05-01 00:00:00

abstract：:Transcriptional network evolution is central to the development of complex biological systems. Networks can evolve through variation of master regulators and/or by changes in regulation of genes within networks. To gain insight into meaningful evolutionary differences in large networks, it is essential to address the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jegga AG,Inga A,Menendez D,Aronow BJ,Resnick MA

更新日期：2008-01-22 00:00:00

abstract：:In humans, spontaneous movements are often preceded by early brain signals. One such signal is the readiness potential (RP) that gradually arises within the last second preceding a movement. An important question is whether people are able to cancel movements after the elicitation of such RPs, and if so until which po...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schultze-Kraft M,Birman D,Rusconi M,Allefeld C,Görgen K,Dähne S,Blankertz B,Haynes JD

更新日期：2016-01-26 00:00:00

abstract：:Mast cells and nerve growth factor (NGF) have both been reported to be involved in neuroimmune interactions and tissue inflammation. In many peripheral tissues, mast cells interact with the innervating fibers. Changes in the behaviors of both of these elements occur after tissue injury/inflammation. As such conditions...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leon A,Buriani A,Dal Toso R,Fabris M,Romanello S,Aloe L,Levi-Montalcini R

更新日期：1994-04-26 00:00:00

abstract：:Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were fur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson CW,Lewis JB,Atkins JF,Gesteland RF

更新日期：1974-07-01 00:00:00

abstract：:Chronic inflammation results in increased nitrogen monoxide (.NO) formation and the accumulation of nitrite (NO(2-)). Neutrophils stimulated by various inflammatory mediators release myeloperoxidase to produce the cytotoxic agent hypochlorous acid (HOCl). Exposure of chondrocytic SW1353 cells to HOCl resulted in a con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whiteman M,Hooper DC,Scott GS,Koprowski H,Halliwell B

更新日期：2002-09-17 00:00:00

abstract：:125I-Labeled L-fucose-albumin complex and rat preputial beta-glucuronidase are rapidly cleared from plasma after intravenous infusion. L-Fucose-albumin retards the plasma clearance of beta-glucuronidase whereas D-fucose-albumin is inactive. In vitro, 125I-labeled L-fucose-albumin is taken up into rat or rabbit alveola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shepherd VL,Lee YC,Schlesinger PH,Stahl PD

更新日期：1981-02-01 00:00:00

abstract：:Citizenship endows legal protections and is associated with economic and social gains for immigrants and their communities. In the United States, however, naturalization rates are relatively low. Yet we lack reliable knowledge as to what constrains immigrants from applying. Drawing on data from a public/private natura...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hainmueller J,Lawrence D,Gest J,Hotard M,Koslowski R,Laitin DD

更新日期：2018-01-30 00:00:00

abstract：:Although microtubules (MTs) frequently form highly dynamic networks, subsets of MTs become stabilized in response to environmental cues and function as specialized tracks for vesicle and macromolecular trafficking. MT stabilization is controlled by specialized plus-end tracking proteins (+TIPs) whose accumulation at t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Naghavi MH,Gundersen GG,Walsh D

更新日期：2013-11-05 00:00:00

abstract：:Human workers ensure the functioning of governments around the world. The efficacy of human workers, in turn, is linked to the climatic conditions they face. Here we show that the same weather that amplifies human health hazards also reduces street-level government workers' oversight of these hazards. To do so, we emp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Obradovich N,Tingley D,Rahwan I

更新日期：2018-08-28 00:00:00

abstract：:Recent analyses of high-throughput protein interaction data coupled with large-scale investigations of evolutionary properties of interaction networks have left some unanswered questions. To what extent do protein interactions act as constraints during evolution of the protein sequence? How does the type of interactio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mintseris J,Weng Z

更新日期：2005-08-02 00:00:00

abstract：:Endosymbiotic acquisition of bacteria by a protist, with subsequent evolution of the bacteria into mitochondria and plastids, had a transformative impact on eukaryotic biology. Reconstructing events that created a stable association between endosymbiont and host during the process of organellogenesis--including establ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nowack EC,Grossman AR

更新日期：2012-04-03 00:00:00

abstract：:The effect of metabolic depletion on the maturation-associated loss of membrane functions has been studied by using sheep reticulocytes incubated in vitro at 37 degrees C for periods up to 41 hr. ATP was either maintained with glucose, adenosine plus inosine, or depleted with 2-deoxyglucose plus arsenate. Two membrane...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weigensberg AM,Blostein R

更新日期：1983-08-01 00:00:00

abstract：:The allocation of carbon and nitrogen resources to the synthesis of plant proteins, carbohydrates, and lipids is complex and under the control of many genes; much remains to be understood about this process. QQS (Qua-Quine Starch; At3g30720), an orphan gene unique to Arabidopsis thaliana, regulates metabolic processes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Zheng W,Zhu Y,Ye H,Tang B,Arendsee ZW,Jones D,Li R,Ortiz D,Zhao X,Du C,Nettleton D,Scott MP,Salas-Fernandez MG,Yin Y,Wurtele ES

更新日期：2015-11-24 00:00:00

abstract：:Trace greenhouse gases are a fundamentally important component of Earth's global climate system sensitive to global change. However, their concentration in the pre-Pleistocene atmosphere during past warm greenhouse climates is highly uncertain because we lack suitable geochemical or biological proxies. This long-stand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beerling DJ,Fox A,Stevenson DS,Valdes PJ

更新日期：2011-06-14 00:00:00

abstract：:We describe in this study punchless, a nonpathogenic mutant from the rice blast fungus M. grisea, obtained by plasmid-mediated insertional mutagenesis. As do most fungal plant pathogens, M. grisea differentiates an infection structure specialized for host penetration called the appressorium. We show that punchless dif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clergeot PH,Gourgues M,Cots J,Laurans F,Latorse MP,Pepin R,Tharreau D,Notteghem JL,Lebrun MH

更新日期：2001-06-05 00:00:00

abstract：:While 9-O-acetylation of sialic acids has been reported in some mammalian tissues, the distribution of this modification on specific cell types and molecules is largely unknown. The influenza C virus hemagglutinin-esterase is a membrane-bound glycoprotein that binds specifically to 9-O-acetylated sialic acids (hemaggl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein A,Krishna M,Varki NM,Varki A

更新日期：1994-08-02 00:00:00

abstract：:In this note the Weierstrass integral J is taken as L, the Riemann integral of length. Here M(n), n > 1, is a compact, connected manifold of class C(infinity) with a positive definite Riemann structure. Presentations (phi, U(phi)) in M(n) and geodesics (with the aid of the Euler-Riemann equations) are defined in Morse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morse M

更新日期：1977-06-01 00:00:00

abstract：:We have postulated that the toxic neuropathies associated with neurofilament-filled axonal swellings have a common pathogenesis, the covalent crosslinking of neurofilaments during anterograde transport. The newly described gamma-diketone, 3,4-dimethyl-2,5-hexanedione (DMHD), is a more potent analogue of the toxic meta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graham DG,Szakál-Quin G,Priest JW,Anthony DC

更新日期：1984-08-01 00:00:00

abstract：:Evidence indicates that the modulatory effects of the adrenergic stress hormone epinephrine as well as several other neuromodulatory systems on memory storage are mediated by activation of beta-adrenergic mechanisms in the amygdala. In view of our recent findings indicating that the amygdala is involved in mediating t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quirarte GL,Roozendaal B,McGaugh JL

更新日期：1997-12-09 00:00:00