当前位置： SCI文献检索 > NEUROSCIENCE LETTERS期刊下所有文献 > Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model.

Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model.

Abstract：

:Hypoxic preconditioning (HP) is a novel strategy to make stem cells resistant to the ischemic environment they encounter after transplantation into injured tissue; this strategy improves survival of both the transplanted cells and the host cells at the injury site. Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced the expression of marker genes in DsRed-engineered neural stem cells (NSCs-DsRed). Similar to untreated AT-MSCs, HP-AT-MSCs had normal morphology and were positive for the cell surface markers CD90, CD105, and CD29, but not CD31. In three in vitro ischemic-mimicking injury models, HP-AT-MSCs significantly increased both the viability of NSCs-DsRed and the expression of DsRed and clearly reduced the number of annexin-V-positive apoptotic NSCs-DsRed and the expression of the apoptotic factor Bax. Consistent with the in vitro assay, co-transplantation of NSCs-DsRed with HP-AT-MSCs significantly improved the survival of the NSCs-DsRed, resulting in an increased expression of the DsRed reporter gene at the transplantation site in a rat spinal cord injury (SCI) model. These findings suggest that the co-transplantation of HP-AT-MSCs with engineered NSCs can improve both the cell survival and the gene expression of the engineered NSCs, indicating that this novel strategy can be used to augment the therapeutic efficacy of combined stem cell and gene therapies for SCI.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Oh JS,Ha Y,An SS,Khan M,Pennant WA,Kim HJ,Yoon DH,Lee M,Kim KN

doi

10.1016/j.neulet.2010.02.008

subject

Has Abstract

pub_date

2010-03-26 00:00:00

pages

215-9

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(10)00154-0

journal_volume

472

pub_type

杂志文章

相关文献

NEUROSCIENCE LETTERS文献大全
  • Relative enrichment of the lighter 59 kDa form of glutamic acid decarboxylase in nerve endings: an immunoblotting study in pituitary neurointermediate lobe.

    abstract：:Previous studies established that glutamate decarboxylase (GAD) is present in the brain of higher vertebrates in two forms composed of the homodimeric association of two subunits 59 and 63 kDa, respectively, that were found to be structurally related. We have performed quantitative comparative immunoblotting of whole ...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(91)90626-5

    authors： Henry S,Tappaz M

    更新日期：1991-10-14 00:00:00

  • Monoamine oxidase activity in chick cerebral microvessels.

    abstract：:In chick brain microvessels, monoamine oxidase activity using catecholamines (dopamine and noradrenaline) and indoleamines (serotonin and tryptamine) as substrates was higher than that measured in synaptosomes and free mitochondria. In contrast, phenylalkyamines such as tyramine and 2-phenylethylamine were more deamin...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(86)90075-3

    authors： Durand M

    更新日期：1986-12-12 00:00:00

  • Increased content of immunoreactive Leu-enkephalin and alteration of delta-opioid receptor in hearts of spontaneously hypertensive rats.

    abstract：:The levels of immunoreactive Leu-enkephalin (ir-Leu-Enk) were measured in acid extract of hearts and lungs of adult normotensive Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats. Heart contents in ir-Leu-Enk were significantly higher in SHR rats (2.67 pmol/g) than in WKY rats (1.5 pmol/g). However, no sign...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(88)90280-7

    authors： Dumont M,Lemaire S

    更新日期：1988-11-22 00:00:00

  • Rapid interaction of tetraethylammonium with delayed K+ channel in mammalian brain neuron.

    abstract：:In order to study the rapidity of interaction between tetraethylammonium (TEA) and the delayed K+ channel of the mammalian brain neuron, TEA was applied to isolated rat hippocampal pyramidal neurons in a time-controlled sequence with a voltage step using a concentration clamp technique. TEA at a concentration of 30 mM...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(91)90601-o

    authors： Oyama Y,Harata N,Akaike N

    更新日期：1991-10-14 00:00:00

  • Growth factor-estradiol interaction on DNA labeling and cytoskeletal protein expression in cultured rat astrocytes.

    abstract：:Growth factors are major signaling agents regulating neuron-glia dialogue. Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), insulin growth factor-I (IGF-I) and insulin (INS) induce neuronal and astroglial cell proliferation and differentiation. This is true also for estrogens that influence astroc...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2004.01.024

    authors： Avola R,Mignini F,Mazzone V,Fisichella A,Zaccheo D,Tomassoni D

    更新日期：2004-04-01 00:00:00

  • Motor awareness and dissociable levels of action representation.

    abstract：:The present study evaluated the performance of a tracking task during which no, a small (subliminal: 20°) or a large (conscious: 60°) rotational perturbation was implemented. The instantaneous as well as carry-over effects of the perturbations were assessed. The subjective reports revealed that the subjects did not di...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2011.02.077

    authors： Serrien DJ,Spapé MM

    更新日期：2011-04-25 00:00:00

  • Increases in antidromic latency of neurohypophyseal neurons during sustained activation.

    abstract：:Antidromic action potentials were recorded from rat paraventricular nucleus (PVN) and supraoptic nucleus neurons following electrical stimulation of the pituitary stalk. Increases in stimulation frequency to 10 or 20 Hz caused progressive increases in antidromic latency and, often, failure of the antidromic potential ...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(83)90437-8

    authors： Pittman QJ

    更新日期：1983-06-30 00:00:00

  • Diagnostic value of NT-proCNP compared to NSE and S100B in cerebrospinal fluid and plasma of patients with sepsis-associated encephalopathy.

    abstract：:Sepsis-associated encephalopathy (SAE) has significant impact on the neurocognitive outcome of sepsis survivors. This study was conducted to analyze the amino-terminal propeptide of the C-type natriuretic peptide (NT-proCNP) as a biomarker for SAE in comparison to neuron-specific enolase (NSE) and S100B protein. Cereb...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2018.11.014

    authors： Ehler J,Saller T,Wittstock M,Rommer PS,Chappell D,Zwissler B,Grossmann A,Richter G,Reuter DA,Nöldge-Schomburg G,Sauer M

    更新日期：2019-01-23 00:00:00

  • An intermediate conductance calcium-activated potassium channel in rat visceral sensory afferent neurons.

    abstract：:Whole-cell and single channel recordings were used to characterize an intermediate conductance calcium-activated potassium (KCa) channel in sensory neurons of the nodose ganglion. From a -80 mV holding potential, the total outward current in these neurons was increased when extracellular calcium was raised from 0.02 t...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(94)91056-1

    authors： Hay M,Kunze DL

    更新日期：1994-02-14 00:00:00

  • Electrophysiological development of embryonic hippocampal neurons from the rat grown on synthetic thin films.

    abstract：:We have studied the electrophysiological properties of hippocampal neurons grown on surfaces of organic thin films formed on glass or silicon substrates and on microelectronic device surfaces in culture. Hippocampal neurons were dissociated from embryonic rats and plated on substrates chemically modified with laminin ...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(97)13372-9

    authors： Offenhäusser A,Sprössler C,Matsuzawa M,Knoll W

    更新日期：1997-02-14 00:00:00

  • Activation of caspase-3 pathway by expression of sGalphai2 protein in BHK cells.

    abstract：:Treatment with dopamine and other dopamine D2 receptor agonists has been shown to induce cell death through activation of caspase-3 pathway. However, initial step that leads to the activation of caspase-3 in D2 receptor-mediated apoptotic pathway remains unclear. Recently, it was shown that a spliced variant of Galpha...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2008.04.078

    authors： López-Aranda MF,Navarro-Lobato I,López-Téllez JF,Blanco E,Masmudi-Martín M,Khan ZU

    更新日期：2008-07-04 00:00:00

  • Different roles of group I and group II metabotropic glutamate receptors on phencyclidine-induced dopamine release in the rat prefrontal cortex.

    abstract：:The dopamine system in the limbic-prefrontal cortex has been assumed to play an important role in the cognitive dysfunction of schizophrenia and phencyclidine (PCP)-induced psychosis. In the present study, the role of metabotropic glutamate (mGlu) receptor subtypes on PCP-induced cortical dopamine release was investig...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(02)01261-2

    authors： Maeda J,Suhara T,Okauchi T,Semba J

    更新日期：2003-01-23 00:00:00

  • Presence of the pineal hormone melatonin in rat cochlea: its variations with lighting conditions.

    abstract：:Within the normal inner ear, there are elements that belong to the immune system. Different inner ear disorders can be explained by autoimmune mechanisms, affecting both humoral and cellular immunity. Melatonin, the principal hormone of the pineal gland, modulates the immune system and extensively participates in the ...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(97)00844-6

    authors： Lopez-Gonzalez MA,Guerrero JM,Delgado F

    更新日期：1997-11-28 00:00:00

  • Mismatch negativity (MMN) to speech sounds is modulated systematically by manual grip execution.

    abstract：:Manual actions and speech are connected: for example, grip execution can influence simultaneous vocalizations and vice versa. Our previous studies show that the consonant [k] is associated with the power grip and the consonant [t] with the precision grip. Here we studied whether the interaction between speech sounds a...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2017.05.024

    authors： Tiainen M,Tiippana K,Paavilainen P,Vainio M,Vainio L

    更新日期：2017-06-09 00:00:00

  • A radioimmunohistochemical method for autoradiographic visualization of cell antigens using 125I-staphylococcal protein A.

    abstract：:125I-Staphylococcal protein A was used to visualize immunoreactive cell antigen in rat brain and pituitary by autoradiography. Autoradiograms of rat brain sections generated with 125I-protein A were clear and showed low background signals. We were able to visualize neural structures containing tyrosine hydroxylase or ...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(88)90368-0

    authors： Correa FM,Guilhaume SS,Saavedra JM

    更新日期：1988-06-29 00:00:00

  • Sodium homeostasis in rat hippocampal slices during oxygen and glucose deprivation: role of voltage-sensitive sodium channels.

    abstract：:The breakdown in brain ionic homeostasis during energy deprivation (anoxic depolarization [AD]) is intimately linked to neuronal injury. We studied the role of one particular route of Na+ influx, voltage-sensitive Na+ channels, in the AD induced by O2 and/or glucose deprivation. We recorded extracellular Na+ concentra...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(99)00728-4

    authors： Fung ML,Croning MD,Haddad GG

    更新日期：1999-11-05 00:00:00

  • The peripheral role of group I metabotropic glutamate receptors on nociceptive behaviors in rats with knee joint inflammation.

    abstract：:We examined whether the mGluR1 and mGluR5 were involved in development and maintenance of behavioral signs of non-evoked pain and secondary mechanical hyperalgesia induced by knee joint inflammation. Selective mGluR1 antagonist, (RS)-1-aminoindan-1,5-dicarboxylic acid (AIDA: 50, 100, 200 microM/25 microl, n=10 per gro...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2007.01.063

    authors： Lee KS,Kim J,Yoon YW,Lee MG,Hong SK,Han HC

    更新日期：2007-04-12 00:00:00

  • Neuron-like cells on the apical surface of the developing rat olfactory epithelium.

    abstract：:Using scanning electron microscopy (SEM), we encountered a new phenomenon in developing olfactory structures. A few cells with single slender processes that sprout from their cell bodies were found lying on the surface of the developing olfactory epithelium in 15 and 16 day old rat embryos. These slender processes res...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(97)00884-7

    authors： Menco BP,Jackson JE

    更新日期：1997-12-19 00:00:00

  • The effects of combining chondroitinase ABC and NEP1-40 on the corticospinal axon growth in organotypic co-cultures.

    abstract：:The area surrounding the injured spinal cord is a non-permissive milieu for axonal growth due to the inhibitory factors, especially chondroitin sulfate proteoglycan (CSPG) and Nogo. Recent studies have reported that chondroitinase ABC (ChABC) or Nogo-66(1-40) antagonist peptide (NEP1-40) promote axonal growth after sp...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2010.03.049

    authors： Nakamae T,Tanaka N,Nakanishi K,Kamei N,Sasaki H,Hamasaki T,Yamada K,Yamamoto R,Izumi B,Ochi M

    更新日期：2010-05-26 00:00:00

  • NADPH-diaphorase reactivity is reduced in the molecular layer but increased in the granular layer of primate cerebellum after prolonged anaesthesia.

    abstract：:To test if nitric oxide (NO) is involved in modulation of neuronal activity after global changes of brain function, nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) reactivity in the cerebellar cortex was compared in monkeys maintained under anaesthesia for 24-48 h with others only anaesthetised for pe...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(97)00497-7

    authors： Yan XX,Garey LJ

    更新日期：1997-07-18 00:00:00

  • Electrophysiological evidence of collateral projections of parabrachio-thalamic relay neurons.

    abstract：:We studied axonal branching of 14 parabrachial nucleus neurons, activated antidromically from 2 of the 3 stimulation sites, i.e. the ipsi- and contralateral thalamic taste area (TTAs) and the ipsilateral central nucleus of the amygdala (CA). Making use of antidromic latencies, collision times and refractory periods at...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(87)90222-9

    authors： Hayama T,Ogawa H

    更新日期：1987-12-16 00:00:00

  • Dynamic characteristics and adaptability of reflex eye movements of Fyn-kinase-deficient mice.

    abstract：:Fyn-kinase is expressed widely in the entire brain, including the cerebellum. Fyn-kinase-deficient mice are known to exhibit hypersensitivity to ethanol. To evaluate the cerebellar functions of Fyn-kinase, we examined the dynamic characteristics of the horizontal optokinetic response (HOKR) and vestibulo-ocular reflex...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(00)00779-5

    authors： Kitazawa H,Katoh A,Yagi T,Nagao S

    更新日期：2000-02-25 00:00:00

  • Autoradiographic study of the cellular localization of [3H]glibenclamide binding sites in the rat hippocampus.

    abstract：:[3H]Glibenclamide, a potent ATP-sensitive K+ channel blocker, is a specific ligand for the identification of the sulfonylureas receptors which are closely related to ATP-sensitive K+ channels. In order to determine the pre- or postsynaptic localization of these receptors in the rat hippocampus, we studied the effects ...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(91)90884-v

    authors： Tremblay E,Zini S,Ben-Ari Y

    更新日期：1991-06-10 00:00:00

  • Brain derived neurotrophic factor does not act on adult human cerebral endothelial cells.

    abstract：:Brain derived neurotrophic factor (BDNF), known to promote survival of neurons, has recently been involved in the regulation of endothelial cells and was shown to possess immunoregulatory properties. We therefore asked whether BDNF has immunoregulating effects in human cerebral endothelial cells (HCEC). Adult HCEC exp...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(02)00778-4

    authors： Ruprecht K,Stadelmann C,Hummel V,Klein O,Brück W,Rieckmann P

    更新日期：2002-09-20 00:00:00

  • Chemokine inhibition in rat stab wound brain injury using antisense oligodeoxynucleotides.

    abstract：:Traumatic injury to the central nervous system (CNS) results in the breakdown of the blood-brain barrier and recruitment of hematogenous cells at the site of injury. The role of chemokines in this process has been well recognized and they have been regarded as promising targets for development of anti-inflammatory the...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/s0304-3940(98)00268-7

    authors： Ghirnikar RS,Lee YL,Li JD,Eng LF

    更新日期：1998-05-08 00:00:00

  • Detection of 5-HT3R-A, a 5-HT3 receptor subunit, in submucosal and myenteric ganglia of rat small intestine using in situ hybridization.

    abstract：:Physiological and radioligand binding studies have demonstrated the existence of 5-HT3 receptors in the enteric nervous system. In order to determine if the cloned 5-HT3 receptor subunit, 5-HT3R-A, was expressed in the enteric nervous system of rats, we have performed in situ hybridization with 33P-labeled cRNA antise...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(94)11170-n

    authors： Johnson DS,Heinemann SF

    更新日期：1995-01-16 00:00:00

  • Birds that feed-on-the-wing have few isthmo-optic neurons.

    abstract：:The isthmo-optic system is less developed in birds feeding-on-the-wing, than in pecking avians. This was suggested previously. By intraocular horseradish peroxidase applications, we studied the central origin of this retino-petal system in thrush, haw finch, swift and swallow. Our data support the assumption on a corr...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(94)90207-0

    authors： Feyerabend B,Malz CR,Meyer DL

    更新日期：1994-11-21 00:00:00

  • Evidence for a role of calcineurin in the development of retinocollicular fine topography.

    abstract：:The fine-tuning of topographically organized projections in sensory systems is strongly influenced by electrical activity and use-dependent modifications in synaptic strength. Since calcineurin (CaN), a Ca(2+)-calmodulin dependent serine/threonine phosphatase has been associated with activity-dependent modifications i...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2010.09.071

    authors： Trindade P,Antonioli-Santos R,Teixeira AC,Lanzillotta-Mattos B,Melibeu AC,Campello-Costa P,Linden R,Serfaty CA

    更新日期：2011-01-03 00:00:00

  • Inhibition of GABA and benzodiazepine receptor binding by penicillins.

    abstract：:Penicillins are thought to be GABA receptor antagonists. In order to determine the affinities of various penicillin derivatives for the GABA receptor, their potencies as inhibitors of specific [3H]GABA binding to rat brain membranes were investigated. All investigated penicillins inhibit specific [3H]GABA binding, wit...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/0304-3940(80)90302-x

    authors： Antoniadis A,Müller WE,Wollert U

    更新日期：1980-07-01 00:00:00

  • Word-induced postural changes reflect a tight interaction between motor and lexico-semantic representations.

    abstract：:A tight coupling between lexico-semantic access and motor control has been established on the basis of neuropsychological, neurophysiological, and behavioral evidence. For example, sensory and motor cortices have been shown to be active when subjects listen to words denoting bodily actions. Kinematic analyses of subje...

    journal_title：Neuroscience letters

    pub_type： 杂志文章

    doi：10.1016/j.neulet.2013.09.021

    authors： Shiller DM,Bourguignon N,Frak V,Nazir T,Cadoret G,Robert M,Lemay M

    更新日期：2013-10-25 00:00:00