Abstract:
:It has been reported that icariin and icariside II, two flavonoid glycosides coming from herba epimedii, which have a closely structural relationship, show some pharmacological effects such as preventing osteoporosis, cancer and depression. The content of natural icariside II is very low in herba epimedii, but it is the main component in vivo after the administration of herba epimedii. More icariside II can be obtained from icariin by enzymatic hydrolysis method than by traditional isolation method. This study focuses on finding a simple and feasible method to prepare icariside II from icariin by enzymatic hydrolysis, so as to meet the request for further pharmacologic actions study. Icariin was obtained successively with 90% ethanol extraction, isolation on macroporous resin and purification on silica gel chromatography. Enzymatic hydrolysis conditions were tested for the bioconversion of icariin into icariside II by orthogonal array design. The structures of isolated icariin and produced icariside II were identified by UV, IR, ESIMS, (1)H NMR, (13)C NMR, and DEPT spectroscope. Enzymatic hydrolysis experiment showed that icariin could be transformed into icariside II with the action of beta-glucosidase and the optimum reaction conditions were determined as follows: 50 degrees C, 0.2 M disodium hydrogen phosphate and citric acid buffer system (pH6.0), the ratio of icariin/enzyme is 1:1 and reaction time 5 h. By using this enzymatic condition, 95.5 mg icariside II (with the purity of 99.1%) was obtained eventually by transforming 200 mg icariin.
journal_name
Fitoterapiajournal_title
Fitoterapiaauthors
Xia Q,Xu D,Huang Z,Liu J,Wang X,Wang X,Liu Sdoi
10.1016/j.fitote.2009.12.006subject
Has Abstractpub_date
2010-07-01 00:00:00pages
437-42issue
5eissn
0367-326Xissn
1873-6971pii
S0367-326X(09)00299-8journal_volume
81pub_type
杂志文章相关文献
FITOTERAPIA文献大全abstract::Extracts of roots of Rumex nepalensis, Berberis aristata, Arnebia benthamii, bark of Taxus wallichiana, Juglans regia and petals of Jacquinia ruscifolia were tested for their antifungal activity against twelve different fungal pathogens. Ethanolic extracts of R. nepalensis and J. ruscifolia extracts showed a broad spe...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2008.06.004
更新日期:2008-12-01 00:00:00
abstract::Although the bioactive compounds in goji have been thoroughly identified and quantified, little information is available on the bitter compounds in the berries, and no systematic works on the substances responsible for their bitterness have been performed. Herein, the substances contributing to the bitterness of berri...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2020.104543
更新日期:2020-06-01 00:00:00
abstract::Aqueous extract of the stem bark of Mangifera indica and ethanolic extract of the roots of Pluchea ovalis has been studied on rat tracheal smooth muscle in vitro. The extract of M. indica at 1, 2 and 4 mg/ml and that of P. ovalis at 0.25, 0.5 and 1 mg/ml relaxed, dose-dependently, the rat tracheal smooth muscle strip ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/s0367-326x(02)00230-7
更新日期:2002-12-01 00:00:00
abstract::A new sulfur-containing iridoid glucoside, named paederoside B, was isolated from the stems of Paederia scandens. The structure of the new compound was elucidated as paederosidyl paederosidate by spectral evidence. ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2006.05.003
更新日期:2006-07-01 00:00:00
abstract::The effect of berberine hydrochloride (BBR) on inducible cyclooxygenase-2 (COX-2) in small intestinal mucosa and related mechanisms was investigated in a rat model of acute endotoxemia. The results showed that lipopolysaccharide (LPS) increased COX-2 expression, whereas SB202190 and BBR curtailed it. LPS increased pho...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2011.05.013
更新日期:2011-10-01 00:00:00
abstract::Reinvestigation of Onopordon ambiguum extract afforded a new eudesmane derivative 1, in addition to the known elemanoid derivative 2. The structures of both compounds were determined by spectroscopic techniques, including 1H-, 13C-NMR, DEPT, 1H-1H and 1H-13C COSY analysis. ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/s0367-326x(01)00366-5
更新日期:2002-02-01 00:00:00
abstract::Antimicrobial screening of Enantia polycarpa bark showed activity of the polar extracts against some microorganisms. ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/s0367-326x(99)00153-7
更新日期:2000-06-01 00:00:00
abstract::The irritant potential of total methanolic extract and five triterpenoids newly isolated from the leaves of Ficus carica investigated by open mouse ear assay. Total methanolic extract, calotropenyl acetate, methyl maslinate and lupeol acetate showed potent and persistent irritant effects. ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/s0367-326x(02)00127-2
更新日期:2002-08-01 00:00:00
abstract::There is growing evidence that motherwort (Leonurus japonicus Houtt.), and Chinese patent medicines derived from motherwort, alleviate postpartum uterine subinvolution, as well as the effects on myocardial and cerebral ischemic injuries. We hypothesized that these beneficial effects of motherwort may be related to ang...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2018.05.002
更新日期:2018-07-01 00:00:00
abstract::NAtural Deep Eutectic Solvents (NADES) are chemically simple but physiologically important plant constituents that exhibit unique solubilizing properties of other metabolites, including bioactive constituents. The high polarity of NADES introduces a challenge in the ability of conventional solid-support based chromato...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2016.04.019
更新日期:2016-07-01 00:00:00
abstract::Wedelia (Sphagneticola trilobata, Syn. Wedelia trilobata), a notoriously invasive agricultural weed, can inhibit the growth and development of crops in the field. Eight new eudesmanolides (1-8), along with twelve known congeners (9-20), were isolated and identified from the flowers of Wedelia. Their chemical structure...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2019.104452
更新日期:2020-04-01 00:00:00
abstract::Crude CH2Cl2 extract from leaves of Piper laevicarpu (Piperaceae) displayed antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi (Y strain) and antimicrobial potential against Cryptococcus gattii (strain-type WM 178). Bioactivity-guided fractionation of crude extract afforded one new natural bio...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2016.04.005
更新日期:2016-06-01 00:00:00
abstract::A new steroidal alkaloid, (-)-veranigrine (1) was isolated from the roots and rhizomes of Veratrum nigrum L. By means of spectroscopic methods its structure was established as (20S, 25S)-iminocholesta-5,22(N)-diene-1beta,3beta-diol. ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2008.09.003
更新日期:2009-01-01 00:00:00
abstract::Seven new 2-(2-phenylethyl)chromone derivatives (1-7) including a chlorinated one (4), together with eight known ones (8-15), were isolated from the EtOAc extract of artificial agarwood originating from Aquilaria sinensis (Lour.) Gilg. All structures including the absolute configurations were unambiguously elucidated ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2016.01.011
更新日期:2016-04-01 00:00:00
abstract::The tea seed triterpene saponin (TS) from Camellia sinensis was found to exhibit better antitumor activity in vivo in S180 implanted ICR mice and QR inducing activity for hepa lclc7 cells respectively compared with the total tea seed saponin (TTS), hydrolysate of the TTS and tea seed flavonoid glycosides (TF). By bioa...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2012.12.022
更新日期:2013-01-01 00:00:00
abstract::Two new benzofuran derivatives, 2-(4'-hydroxybenzyl)-5,6-methylenedioxy-benzofuran (1) and 2-(4'-hydroxybenzoyl)-5,6-methylenedioxy-benzofuran (2), along with 8 known compounds were isolated from 60% EtOH extract of the fibrous roots of Liriope spicata var. prolifera. Their structures were elucidated on the basis of e...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2010.09.002
更新日期:2011-03-01 00:00:00
abstract::Phytochemical investigation on the stem bark of Toona sinensis was carried out by various chromatographic techniques resulting in the isolation and elucidation of two novel tirucallane triterpenoids, named (20S)-3-oxo-tirucalla-25-nor-7-en-24-oic acid (1) and (20S)-5α,8α-epidioxy-3-oxo-24-nor-6.9(11)-dien-23-oic acid ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2016.05.009
更新日期:2016-07-01 00:00:00
abstract::Five new megastigmane glycosides, urenalobasides A-E (1-5), together with 11 known ones (6-16) were isolated from Urena lobata. Their structures were determined by extensive spectroscopic and spectrometric data (1D and 2D NMR, IR, and HRESIMS) and calculated electronic circular dichroism method. Compounds 1 and 2 are ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2018.02.017
更新日期:2018-06-01 00:00:00
abstract::Zuojinwan, which consists of Rhizoma coptidis-Evodia rutaecarpa powder (6:1,g/g), is a traditional Chinese medicine (TCM) prescription, clinically used for the treatment of gastro-intestinal disorders. This study compared the pharmacokinetics of dehydroevodiamine and coptisine, the main active ingredients in Zuojinwan...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2011.07.012
更新日期:2011-12-01 00:00:00
abstract::The effects of aqueous extracts of Celastrus paniculatus (CP) seeds were shown to have antioxidant properties in rats. In the study reported here, we have investigated the free radical scavenging capacity of three aqueous extracts (WSEs) obtained from CP seeds: a room temperature extract (WF); a hot water extract (HF)...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/s0367-326x(03)00190-4
更新日期:2003-12-01 00:00:00
abstract::Zuo-Jin-Wan (ZJW) has been used to treat hepatocellular carcinoma in Asia. This study was to determine whether ZJW and its components blocked activator protein 1 (AP-1) and nuclear factor-κB (NF-κB) activities as well as tumor promotion in hepatoblastoma HepG2 cells. ZJW and its components, Coptis chinensis and Evodia...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2011.02.009
更新日期:2011-06-01 00:00:00
abstract::A series of novel myricetin derivatives containing benzimidazole skeleton were constructed. The structure of compound 4g was further corroborated via X-ray single crystal diffractometer. The antimicrobial bioassays showed that all compounds exhibited potent inhibitory activities against Xanthomonas axonopodis pv. Citr...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2020.104804
更新日期:2020-12-11 00:00:00
abstract::Imperatorin (Imp) as a hypotensive active ingredient, its hypotensive effect was evaluated in the SHRs, its calcium antagonism and affinity to L-type calcium channel was also confirmed. The results showed that the blood pressure was decreased in the SHRs treated with Imp, the aortic ring was relaxed with Imp, L-type c...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2011.05.016
更新日期:2011-10-01 00:00:00
abstract::A simple, sensitive and accurate liquid chromatographic method with photodiode-array detection was developed for determination of dehydroevodiamine with detection wavelength at 368 nm and column temperature at 30 degrees C. The separation was carried out on an Agilent Zorbax SB-C(18) column (250 mm x 4.6 mm, 5 microm)...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2009.05.017
更新日期:2009-10-01 00:00:00
abstract::Black carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) is a valuable source of carbohydrates, minerals and vitamins and contains also high amounts of anthocyanins giving the characteristic deep-purple color. These latter compounds are known as natural dyes used in the food and pharmaceutical industry that ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2017.10.006
更新日期:2018-01-01 00:00:00
abstract::The objective of this study was to evaluate potential hepatoprotective capabilities of quercetin in relation to its modulation of the HO-1 and NOS-2 activities in an experimental model of fulminant liver failure. Liver insult was induced by in vivo administration of D-galactosamine (d-GalN, 400 mg/kg, i.p.) and lipopo...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2013.03.016
更新日期:2013-06-01 00:00:00
abstract::The antifungal activity of fresh, aqueous Brassica oleracea var. botrytis juice against Candida albicans and other pathogenic fungi was investigated. The juice was found to be effective both in inhibiting the growth of blastoconidia and reducing the appearance of C. albicans germ tubes. Furthermore, the juice inhibite...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/s0367-326x(03)00108-4
更新日期:2003-07-01 00:00:00
abstract::Two triglycerides, both bearing polyunsaturated fatty acid residues, were isolated from the hexane extract of Urena lobata. One of them is characterized by the presence of three different polyunsaturated fatty acids. Their structures were studied with spectral methods. ...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2006.03.010
更新日期:2006-06-01 00:00:00
abstract::A new C(18)-phytosphingosine ceramide containing non-hydroxy fatty acid, armillaramide (1), has been isolated together with ergosterol peroxide from the fruiting bodies of the basidiomycete Armillaria mellea. Its structure was established as (2S,3S,4R)-2-hexadecanoylamino-octadecane-1,3,4-triol by spectroscopic and ch...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/s0367-326x(01)00319-7
更新日期:2001-12-01 00:00:00
abstract::Two novel 2-arylbenzofuran dimers, shandougenines A (1) and B (2), and two new polyisoprenylated flavanones 3 (shandougenine C) and 4 (shandougenine D) were isolated from the 95% EtOH extract of Sophora tonkinensis, together with 18 known compounds. Their structures were determined on the basis of spectral data interp...
journal_title:Fitoterapia
pub_type: 杂志文章
doi:10.1016/j.fitote.2014.08.019
更新日期:2014-12-01 00:00:00