Abstract:
BACKGROUND AIMS:Cytokine-induced killer (CIK) cells have shown cytolytic activity against several tumor cells in vitro and in animal tumor models. Furthermore, CIK cells activated by dendritic cell (DC) stimulation show increased anti-tumor activity. This study aimed to evaluate the clinical efficacy of DC-activated CIK cell treatment following regular chemotherapy and the effects of this therapy on immune responses in patients with non-small cell lung cancer (NSCLC) after surgery. METHODS:A paired study, with 42 patients in each group with stage I-IIIa NSCLC after surgery, was performed. Patients received chemotherapy alone (CT) or chemotherapy and DC-activated CIK cell treatment (immuno-CT). Disease-free survival (DFS) and overall survival were evaluated. CIK cell cytotoxicity against tumor cells was detected using a lactate dehydrogenase-based method. Serum cytokine levels in the immuno-CT group were detected using cytokine antibody arrays. RESULTS:The cytotoxicity of CIK cells was significantly enhanced by DC activation. The 2-year overall survival rate in the immuno-CT group was significantly improved compared with the CT group (94.7 +/- 3.6% versus 78.8 +/- 7.0%, P < 0.05). The 2-year DFS of these two groups showed no significant difference. DC-activated CIK cell treatment increased production of cytokines that have known anti-tumor effects, including IFN-gamma, MIG, TNF-alpha and TNF-beta, in patients who had no progression, but they were not found in patients who developed recurrence/metastasis. CONCLUSIONS:This study suggests that the role of DC-activated CIK cells in improvement of chemotherapy for malignant tumor treatment is associated with up-regulation of the production of cytokines involved in the anti-tumor effect.
journal_name
Cytotherapyjournal_title
Cytotherapyauthors
Li H,Wang C,Yu J,Cao S,Wei F,Zhang W,Han Y,Ren XBdoi
10.3109/14653240903121252subject
Has Abstractpub_date
2009-01-01 00:00:00pages
1076-83issue
8eissn
1465-3249issn
1477-2566pii
S1465-3249(09)70355-Xjournal_volume
11pub_type
临床试验,杂志文章相关文献
CYTOTHERAPY文献大全abstract:BACKGROUND:The aim of this study was to investigate preservation of anti-leukemic activity and protection from opportunistic infections after transplantation of allogeneic + cells in patients with hematologic malignancies and bad prognosis. Methods Thirty-three patients [median age 42 years, range 23-55 years, diagnosi...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240410005375
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Hematopoietic progenitor stem cells (HPSC) are a specialized transfusion product used for transplantation. Microbial contamination may occur during harvest or subsequent manipulation of these cells. The same difficulties in ensuring a safe, sterile, final product are faced in the preparation of other cell-th...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240310003044
更新日期:2003-01-01 00:00:00
abstract::Cellular & Gene Therapies (CGTs) are complex products, which have been key foci of the International Society for Cell & Gene Therapy (ISCT). For this ISCT North American Legal & Regulatory Affairs Committee review publication, CGTs include but are not limited to somatic cell-based therapies, pluripotent cell-derived c...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2019.04.002
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND AIMS:Umbilical cord blood (UCB) is a rich source of stem cells, the characterization and isolation of which requires specific stem cell markers and reliable and reproducible protocols. METHODS:We assessed CD133 isolation in 39 UCB samples, using a commercial immunomagnetic cell-sorting protocol, and, becaus...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653249.2012.663487
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND AIMS:Enumeration of viable CD34(+) cells provides critical information for the bone marrow (BM) transplant physician. The single-platform ISHAGE protocol is the most reliable method currently available to quantitate accurately this important subset of cells. Previous studies have shown that 5 CD34(+) cells/m...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240902923161
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Mesenchymal stromal cells (MSC) are pluripotent progenitor cells that can be found in human bone marrow (BM). These cells have low immunogenicity and could suppress alloreactive T-cell responses. In the current study, MSC were tested for their capacity to carry and deliver the erythropoietin (EPO) gene in vi...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240701816996
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:The isolation of lymphocytes - and removal of platelets (PLTs) and red blood cells (RBCs) - from an initial blood sample prior to culture is a key enabling step for effective manufacture of cellular therapies. Unfortunately, currently available methods suffer from various drawbacks, including low cell recove...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2018.12.004
更新日期:2019-02-01 00:00:00
abstract::Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous cultur...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2012.11.011
更新日期:2013-03-01 00:00:00
abstract::Chimeric antigen receptor (CAR) T-cell therapy is an investigational immunocellular therapy that reprograms a patient's cytotoxic T cells to engage and eliminate malignant cells. CAR T-cell therapies targeting the CD19 antigen have demonstrated high efficacy in clinical trials for patients with B-cell malignancies and...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2017.06.001
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND AIMS:The immunomodulatory and anti-inflammatory effects of mesenchymal stromal cells (MSC) could prove to be a potential therapeutic approach for prolongation of survival of cell xenotransplantation. Adipose (Ad) MSC from genetically modified pigs could be an abundant source of pig donor-specific MSC. METHO...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653249.2011.651529
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:Transplantation of mesenchymal stem cells (MSC) in rodent models has proved to be an effective therapeutic approach for spinal cord injury (SCI). However, further studies in primate models are still needed before clinical application of MSC to patients. METHODS:MSC were isolated from rhesus monkey BM and in...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240600760808
更新日期:2006-01-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) are a type of adult stem cell that has been exploited for the treatment of a variety of diseases, including cancer. In particular, MSCs have been studied extensively for their ability to treat glioblastoma (GBM), the most common and deadly form of brain cancer in adults. MSCs are attra...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2017.02.002
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been applied to patients in cell therapy for various diseases. Recently, we introduced a novel MSC separation filter device which could yield approximately 2.5-fold more MSCs from bone marrow in a closed system compared with the conventional open density gradient ce...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.10.013
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND AIMS:Cytotherapy is a promising option for neurodegenerative disease treatment. Because of the fatal prognosis and imperative need for effective treatment, amyotrophic lateral sclerosis (ALS) patients request this therapy before its effectiveness has been verified. The increase in clinics offering cytotherap...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653241003774037
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) is an alternative graft source for hematopoietic stem cell transplantation and has been shown to give results comparable to transplantation with other stem cell sources. Donor lymphocyte infusion (DLI) is an effective treatment for relapsed malignancies after hematopoietic stem cel...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.08.001
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND AIMS:Umbilical cord blood transplantation (UCBT) is increasingly used to treat acute leukemias. UCB units are thawed and infused in their entirety at transplant, precluding later use as immunotherapy to prevent or treat leukemia relapse. METHODS:We developed a device that selectively thaws only 1 mL of the ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2015.07.020
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND AIMS:Adoptive immunotherapy with the use of chimeric antigen receptor (CAR)-engineered T cells specific for CD19 has shown promising results for the treatment of B-cell lymphomas and leukemia. This therapy involves the transduction of autologous T cells with a viral vector and the subsequent cell expansion. ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.06.003
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND AIMS:Periodontal tissue regeneration with the use of mesenchymal stromal cells (MSCs) has been regarded as a future cell-based therapy. However, low survival rates and the potential tumorigenicity of implanted MSCs could undermine the efficacy of cell-based therapy. The use of conditioned media from MSCs (MS...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.11.009
更新日期:2015-04-01 00:00:00
abstract::There is rapidly increasing interest in the use of magnetic resonance imaging (MRI) to track cell migration in vivo. Iron oxide MR contrast agents can be detected at micromolar concentrations of iron, and offer sufficient sensitivity for T2*-weighted imaging. Cellular MRI shows potential for assessing aspects of cardi...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240802165699
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND AIMS:CD1d-restricted invariant natural killer (iNK) T cells are rare regulatory T cells that may contribute to the immune-regulation in allogeneic stem cell transplantation (ASCT). Here, we sought to develop an effective strategy to expand human iNK T cells for use in cell therapy to prevent graft-versus-hos...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2018.05.007
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND AIMS:Adipose tissue is a rich and very convenient source of cells for regenerative medicine therapeutic approaches. However, a characterization of the population of adipose-derived stromal and stem cells (ASCs) with the greatest therapeutic potential remains unclear. Under the authority of International Fede...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.02.006
更新日期:2013-06-01 00:00:00
abstract::For more than a decade, Ab conjugated to a radionuclide emitting particulate radiation has been used in the management of leukemia in an effort to deliver targeted doses of radiation to BM, spleen and other sites of disease, while sparing normal organs. This radioimmunotherapy (RIT) approach has been employed to achie...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240701679881
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND AIMS:The immunomodulatory properties of mesenchymal stromal cells (MSCs), together with their tissue regenerative potential, make them interesting candidates for clinical application. METHODS:In the current study, we analyzed the in vitro immunomodulatory effects of MSCs derived from bone marrow (BM-MSCs) a...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2016.07.006
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND AIMS:Endoscopy is the gold standard for the diagnosis and follow-up of patients with Crohn disease (CD). However, a less invasive approach is now being sought for the management of these patients. The objective of this study was to examine whether (18)F-fluorodeoxyglucose (FDG)-positron emission tomography (...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章
doi:10.1016/j.jcyt.2015.10.010
更新日期:2016-02-01 00:00:00
abstract::In the current article we summarize the 15-year experience of the Spanish Cell Therapy Network (TerCel), a successful collaborative public initiative funded by the Spanish government for the support of nationwide translational research in this important area. Thirty-two research groups organized in three programs devo...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2019.11.001
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Identification of HLA class I-restricted CMV epitopes, and the subsequent synthesis of HLA class I-peptide tetrameric complexes, have provided investigators with an important tool for visualising and quantifying the precise in vivo CTL response to CMV reactivation following stem cell transplantation. In conj...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/146532402317251518
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:The efficiency of chimeric antigen receptor (CAR) T-cell-based therapies depends on a sufficient expansion of CAR T cells in vivo and can be weakened by intra-tumoral suppression of CAR T cell functions, leading to a failure of therapy. For example, certain B-cell malignancies such as chronic lymphocytic leu...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2020.08.005
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND AIMS:Acute liver failure (ALF), a life-threatening disease characterized by the sudden loss of hepatic function, can occur after an accidental or intentional acetaminophen overdose. METHODS:With the use of an ALF mouse model, we examined both the preventive and therapeutic potential of intravenously adminis...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.05.018
更新日期:2014-09-01 00:00:00
abstract:BACKGROUND:The possible functional role of basic fibroblast growth factor (bFGF) in regulating the mitotic and metabolic activity of primary human articular chondrocytes was investigated. METHODS:[EF1]Chondrocytes were enzymatically isolated from femoral head cartilage, and were cultured in vitro in monolayer. bFGF-de...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240601182846
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240701656053
更新日期:2007-01-01 00:00:00