Abstract：
:Postmitotic neurons were generated from the human NT2 teratocarcinoma cell line in a novel cell aggregate differentiation procedure. The NT2 model neurons express punctate immunoreactivity for synapsin and for cell markers related to GABAergic and glutamatergic neurotransmission. Using the outside-out patch-clamp configuration, we characterized the kinetics of currents elicited by a rapid application of the amino acid neurotransmitters. Moreover, we detected spontaneous postsynaptic currents in glia free cell cultures that may result from the firing activity of glutamatergic and GABAergic NT2 neurons. These cultured spontaneously active networks may be a useful tool to analyze factors that modulate the formation and efficacy of synapses between human neurons.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Podrygajlo G,Song Y,Schlesinger F,Krampfl K,Bicker Gdoi
10.1016/j.neulet.2009.10.092subject
Has Abstractpub_date
2010-01-14 00:00:00pages
207-10issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(09)01449-9journal_volume
468pub_type
杂志文章abstract：:Sphingosine-1-phosphate (S1P) has been demonstrated to be an important regulator of cell death and survival. Although it has been suggested that the sphingolipid may act as a neuroprotector in the cell apoptosis induced by traumatic brain injury, the mechanisms involved in this action are unknown. In this study, the r...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.12.070
更新日期：2010-02-12 00:00:00
abstract：:The somatosensory system is capable of functional reorganization following peripheral denervation or training. Studies on human amputees with phantom limb pain provided evidence that these reorganizational changes are modulated through nociceptive input. In the present study we used magnetoencephalographic recordings ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01752-3
更新日期：2001-02-09 00:00:00
abstract：:Cultures were produced from retinas taken from human foetuses. Specific cells in culture have the capacity to take up exogenous serotonin, which was then visualized by immunofluorescence using an antisera to serotonin. The known serotonergic uptake blocker, chlorimipramine, inhibited the uptake of the amine while benz...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90025-4
更新日期：1985-04-09 00:00:00
abstract：:Gangliosides are major components of cell membranes and are particularly enriched in the mammalian brain where they represent the major lipid constituents of the neuronal cell surface. In the central nervous system, gangliosides have a close connection to many neurophysiological functions related to neurogenesis, prol...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.05.020
更新日期：2008-07-11 00:00:00
abstract：:Nerve guide implants approved for human application in the peripheral nervous system generally fail to bridge lesion gaps longer than 2 cm and cannot match the clinical performance of autologous nerve transplants. Since current synthetic implants are simply hollow tubes, we aim to recreate the native microarchitecture...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.08.028
更新日期：2010-10-29 00:00:00
abstract：:MicroRNA-146a (miRNA-146a) is an inducible, 22 nucleotide, small RNA over-expressed in Alzheimer's disease (AD) brain. Up-regulated miRNA-146a targets several inflammation-related and membrane-associated messenger RNAs (mRNAs), including those encoding complement factor-H (CFH) and the interleukin-1 receptor associate...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.05.044
更新日期：2011-07-20 00:00:00
abstract：:The ability of increases in extracellular potassium ([K+]o) and/or decreases in extracellular calcium ([Ca2+]o) to induce epileptiform activity in hippocampal slices was studied by systematically varying [K+]o and [Ca2+]o. Slices prepared from kindled rats, both 1 week and 1 month after the last kindled seizure, showe...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90478-8
更新日期：1988-06-17 00:00:00
abstract：:This study investigated the effects of ketamine on fluoxetine-induced antidepressant behavior using the forced swimming test (FST) in mice. In order to understand the possible role of N-methyl-d-aspartate (NMDA) neurotransmission in the antidepressant effect of fluoxetine, different groups of mice (n=10) were administ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.01.015
更新日期：2014-04-30 00:00:00
abstract：:The comorbidity between migraine and depression not only provides a major treatment challenge, but also represents a heavy burden on society. However, the relationship between depression and migraine and their molecular biological mechanisms remain unclear. This study investigated the effects of depression elicited by...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.04.038
更新日期：2013-09-13 00:00:00
abstract：:Secretion of alpha-melanophore-stimulating hormone (alpha-MSH) from the neuroendocrine melanotrope cells in the intermediate lobe of the pituitary gland of the clawed frog Xenopus laevis is regulated by various inhibitory, stimulatory and autocrine factors. The neuropeptide sauvagine stimulates alpha-MSH secretion by ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.11.084
更新日期：2005-03-29 00:00:00
abstract：:The present study examined effects of high altitude exposure, which leads to development of hypoxia, on epiplexus cells associated with the choroid plexus in adult rats. At 2-5 h following exposure, epiplexus cells expressing inducible nitric oxide synthase (iNOS) were commonly observed. Increased expression of comple...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01070-3
更新日期：2000-05-19 00:00:00
abstract：:Neuronal cell cycle activation has been implicated in neurodegenerative diseases such as Alzheimer's disease, while the initiating mechanism of cell cycle activation remains to be determined. Interestingly, our previous studies have shown that cell cycle activation by c-Myc (Myc) leads to neuronal cell death which sug...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.10.004
更新日期：2011-11-14 00:00:00
abstract：:Effects of anxiety on perceptual-motor organisation of rhythmical forearm movements were examined using an interrupted time series design with staggered baselines. Participants were exposed to repeated baseline sessions interrupted with two anxiety-inducing sessions. Results showed that under moderate levels of anxiet...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.04.036
更新日期：2005-08-12 00:00:00
abstract：:The MgATP-dependent priming step of exocytosis has been suggested to be regulated negatively by GTP-binding protein G0 in permeabilized adrenal chromaffin cells. We have reported that synaptosomal-associated protein of 25 kDa (SNAP-25) and vesicle-associated membrane protein 2 (VAMP-2) form a complex in chromaffin cel...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00608-3
更新日期：1997-09-05 00:00:00
abstract：:The ability to extract invariant relationships from physically varying stimulation is critical for example to categorical perception of complex auditory information such as speech and music. Human subjects were presented with tone pairs randomly varying over a wide frequency range, there being no physically constant t...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00237-2
更新日期：1999-04-23 00:00:00
abstract：:Sciatic axotomy in 2-day-old rats (P2) causes lumbar motoneuron loss, which could be associated with nitric oxide (NO) production. NO may be produced by three isoforms of synthase (NOS): neuronal (nNOS), endothelial (eNOS) and inducible (iNOS). We investigated NOS expression and NO synthesis in the lumbar enlargement ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.08.035
更新日期：2006-10-23 00:00:00
abstract：:The high-affinity of [3H]gamma-aminobutyric acid (GABA) to GABAA receptors and [3H]baclofen to GABAB receptors were studied in the cerebellum of pyridoxine-deficient rats and compared to pyridoxine-supplemented controls. There was a significant increase in the maximal binding (Bmax) of both GABAA and GABAB receptors w...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(84)90056-9
更新日期：1984-08-10 00:00:00
abstract：:Vesicular acetylcholine transporter (VAChT) function is essential for organismal survival, mediating the packaging of acetylcholine (ACh) for exocytotic release. However, its expression pattern in the Drosophila brain has not been fully elucidated. To investigate the localization of VAChT, we developed an antibody aga...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2017.02.012
更新日期：2017-03-16 00:00:00
abstract：:Several studies have suggested that the activity of nitric oxide synthase (NOS) may be involved in the regulation of food intake in the genetically obese Zucker rats. In the present study, we investigated the expression of NOS in various hypothalamic regions of obese and lean Zucker rats using nicotinamide adenine din...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01428-2
更新日期：2000-09-29 00:00:00
abstract：:Extracellular choline-dependency of acetylcholine (ACh) synthesis was examined in rat hippocampal slices. In the presence of hemicholinium-3 (HC-3, 5 microM), extracellular choline-dependent ACh synthesis appeared to consist of two different components. The first component (saturated at up to 50 microM choline) was de...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90039-6
更新日期：1989-10-23 00:00:00
abstract：:Rim is a multi-domain, active zone protein that regulates exocytosis and is implicated in vesicle priming and presynaptic plasticity. We recently demonstrated that synaptic defects associated with loss of Caenorhabditis elegans Rim (termed UNC-10) are accompanied by a reduction in docked vesicles adjacent to the presy...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.08.026
更新日期：2008-10-24 00:00:00
abstract：:Autosomal-dominant familial amyotrophic lateral sclerosis (FALS) is associated with mutation in the gene that encodes Cu/Zn superoxide dismutase (SOD1). We identified a novel missense mutation of SOD-1 (Cys6Gly) in exon 1 in a Japanese woman and her family. The illness showed rapid progression similarly to the FALS wi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00803-4
更新日期：1999-12-03 00:00:00
abstract：:Early intervention following combat deployment has the potential to prevent posttraumatic stress disorder (PTSD), but there is a need for greater understanding of the factors that contribute to PTSD symptom progression. This study investigated: (1) fear-potentiated startle during a fear extinction, (2) white matter mi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2016.02.041
更新日期：2016-04-08 00:00:00
abstract：:The frog horizontal monocular optokinetic nystagmus (OKN) is asymmetrical, the temporal-nasal (T-N) stimulation being the sole stimulation efficient to evoke the reflex, the nasal-temporal (N-T) component being almost absent. Coil recordings showed that, in adult animals, prolonged monocular visual deprivation by unil...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90401-r
更新日期：1992-03-30 00:00:00
abstract：:Reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, play essential roles in physiological plasticity and are also involved in the pathogenesis of persistent pain. Roles of peripheral and spinal ROS in pain have been well established, but much less is known about ROS in the amygdala, a brain region...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2016.03.015
更新日期：2016-04-21 00:00:00
abstract：:The hippocampus is a brain structure specifically vulnerable to short periods of transient cerebral ischemia, and which displays delayed neuronal necrosis. Protein ubiquitination is a posttranslational modification of proteins and an important factor in heat shock response and a regulator of ATP-dependent protein degr...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90389-3
更新日期：1989-01-30 00:00:00
abstract：:We observed the changes of body temperature and the cytokine expressions in the hypothalamus of rats to investigate the effect and mechanism of antipyretic action of acupuncture. Lipopolysaccharide (LPS, i.p., 2.5 mg/kg) was injected into rats and manual acupuncture was performed on Shaofu (HT8), Zutonggu (BL66) or Xi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(01)02538-1
更新日期：2002-02-08 00:00:00
abstract：:Auditory steady-state responses (ASSRs) are increasingly used in research of neuropsychiatric disorders and for brain-computer interface applications. However, results on attentional modulation of ASSRs are inconclusive. The evaluation of large-scale effects of task-related modulation on ASSRs might give better estima...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2018.03.033
更新日期：2018-05-01 00:00:00
abstract：:It has been shown that orexin neuropeptides contribute to morphine-induced physical dependence. The locus coeruleus (LC), which receives a dense extra-hypothalamic orexinergic projection, is a key brain region implicated in the expression of somatic signs of morphine withdrawal syndrome. The aim of the present study i...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.07.050
更新日期：2010-10-04 00:00:00
abstract：:Activin A levels are elevated in the cerebrospinal fluid (CSF) of patients with meningitis and in the sera of patients with sepsis. The source(s) of the elevated concentrations of activin A in CSF and serum have not yet been discovered. Here we demonstrate that primary mouse microglial cells and peritoneal macrophages...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.11.065
更新日期：2007-02-21 00:00:00
