Abstract:
:The MgATP-dependent priming step of exocytosis has been suggested to be regulated negatively by GTP-binding protein G0 in permeabilized adrenal chromaffin cells. We have reported that synaptosomal-associated protein of 25 kDa (SNAP-25) and vesicle-associated membrane protein 2 (VAMP-2) form a complex in chromaffin cells, and the complex dissociates during MgATP-dependent priming. In this study, we examined whether G0 controls such dissociation of the SNAP-25/VAMP-2 complex in the regulation of priming. In digitonin-permeabilized cells, MgATP-gamma-S which can be a phosphate donor for protein phosphorylation failed to cause priming and dissociation of the SNAP-25/VAMP-2 complex. Mastoparan, which directly activates G0, selectively inhibited priming and blocked dissociation of the SNAP-25/VAMP-2 complex. These results suggest that ATP hydrolysis and dissociation of the SNAP-25/VAMP-2 complex are responsible for priming. These results also suggest that dissociation of the complex is one of the sequential steps for priming controlled by G0.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Misonou H,Ohara-Imaizumi M,Kumakura Kdoi
10.1016/s0304-3940(97)00608-3subject
Has Abstractpub_date
1997-09-05 00:00:00pages
182-4issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(97)00608-3journal_volume
232pub_type
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