Real-time imaging of histone H4 hyperacetylation in living cells.

abstract：:Wnts regulate important intracellular signaling events, and dysregulation of the Wnt pathway has been linked to human disease. Here, we uncover numerous Wnt canonical effectors in human platelets where Wnts, their receptors, and downstream signaling components have not been previously described. We demonstrate that th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steele BM,Harper MT,Macaulay IC,Morrell CN,Perez-Tamayo A,Foy M,Habas R,Poole AW,Fitzgerald DJ,Maguire PB

更新日期：2009-11-24 00:00:00

abstract：:KRAS interacts with the inner leaflet of the plasma membrane (PM) using a hybrid anchor that comprises a lysine-rich polybasic domain (PBD) and a C-terminal farnesyl chain. Electrostatic interactions have been envisaged as the primary determinant of interactions between KRAS and membranes. Here, we integrated molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou Y,Prakash PS,Liang H,Gorfe AA,Hancock JF

更新日期：2021-02-09 00:00:00

abstract：:Heparin is the most widely prescribed biopharmaceutical in production globally. Its potent anticoagulant activity and safety makes it the drug of choice for preventing deep vein thrombosis and pulmonary embolism. In 2008, adulterated material was introduced into the heparin supply chain, resulting in several hundred d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss RJ,Spahn PN,Toledo AG,Chiang AWT,Kellman BP,Li J,Benner C,Glass CK,Gordts PLSM,Lewis NE,Esko JD

更新日期：2020-04-28 00:00:00

abstract：:Tyrosine hydroxylase (EC 1.14.16.2), the enzyme catalyzing the rate-limiting step in catecholamine biosynthesis, was localized by electron microscopy within noradrenergic neurons of the nucleus locus coeruleus of the rat brain with a specific antibody to tyrosine hydroxylase labeled by the peroxidase-antiperoxidase im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pickel VM,Joh TH,Reis DJ

更新日期：1975-02-01 00:00:00

abstract：:The mechanism of contrast enhancement of tumors using magnetic resonance imaging was investigated in MCF7 human breast cancer implanted in nude mice. Dynamic contrast-enhanced images recorded at high spatial resolution were analyzed by an image analysis method based on a physiological model, which included the blood c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furman-Haran E,Margalit R,Grobgeld D,Degani H

更新日期：1996-06-25 00:00:00

abstract：:Let t --> gamma(t) be a homogeneous curve in R(n). For suitable f, define [unk](f)(x) = sup(h > 0)|(1/h) [unk](0) (h)f(x - gamma(t)) dt|. Then parallel[unk](f) parallel(p)

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stein EM

更新日期：1976-07-01 00:00:00

abstract：:When human genome was fractionated by thermal elution chromatography, repetitious DNA was found in every arbitrary temperature segment. When these repetitious DNA families were used for in situ hybridization, the following conclusions were evident: (a) Unlike the case of mouse, where essentially all centromeric hetero...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu TC,Arrighi FE,Saunders GF

更新日期：1972-06-01 00:00:00

abstract：:Chlorophyll d-producing cyanobacteria are a recently described group of phototrophic bacteria that is a major focus of photosynthesis research, previously known only from marine environments in symbiosis with eukaryotes. We have discovered a free-living member of this group from a eutrophic hypersaline lake. Phylogene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller SR,Augustine S,Olson TL,Blankenship RE,Selker J,Wood AM

更新日期：2005-01-18 00:00:00

abstract：:In the absence of any overt task performance, it has been shown that spontaneous, intrinsic brain activity is expressed as systemwide, resting-state networks in the adult brain. However, the route to adult patterns of resting-state activity through neuronal development in the human brain is currently unknown. Therefor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fransson P,Skiöld B,Horsch S,Nordell A,Blennow M,Lagercrantz H,Aden U

更新日期：2007-09-25 00:00:00

abstract：:Hydrogen/deuterium exchange followed by trapping of the labeled species in the aprotic solvent DMSO has been used to elucidate structure in both the burst-phase molten globule-folding intermediate of apomyoglobin and in an equilibrium intermediate that models the kinetic intermediate. Precise estimates can be made of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishimura C,Dyson HJ,Wright PE

更新日期：2005-03-29 00:00:00

abstract：:Intrinsic horizontal axon collaterals in the striate cortex of adult cats specifically link columns having the same preferred orientation; consequently, retrograde tracer injections result in intrinsic labeling that is sharply clustered. We have previously shown that the normal development of this circuitry involves t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Callaway EM,Katz LC

更新日期：1991-02-01 00:00:00

abstract：:Variation in the masticatory behavior of hunter-gatherer and agricultural populations is hypothesized to be one of the major forces affecting the form of the human mandible. However, this has yet to be analyzed at a global level. Here, the relationship between global mandibular shape variation and subsistence economy ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： von Cramon-Taubadel N

更新日期：2011-12-06 00:00:00

abstract：:Although some important advances in the modeling of sorption and hygrothermal deformations of nanoporous materials such as hydrated cement paste, shale, coal, and some other rocks and soils have already been made, a comprehensive nanoporomechanics theory remains elusive. Here we strive to formulate it based on Gibb's ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen H,Rahimi-Aghdam S,Bažant ZP

更新日期：2020-02-18 00:00:00

abstract：:Most small multicopy antibiotic-resistance plasmids of Staphylococcus aureus contain a major axis of hyphenated dyad symmetry (palA) that is required for normal replication and stability, although located outside of the minimal replicon. Rearrangements affecting palA cause plasmid instability, a marked reduction in co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gruss AD,Ross HF,Novick RP

更新日期：1987-04-01 00:00:00

abstract：:Reproducible quantification of large biological cohorts is critical for clinical/pharmaceutical proteomics yet remains challenging because most prevalent methods suffer from drastically declined commonly quantified proteins and substantially deteriorated quantitative quality as cohort size expands. MS2-based data-inde...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen X,Shen S,Li J,Hu Q,Nie L,Tu C,Wang X,Poulsen DJ,Orsburn BC,Wang J,Qu J

更新日期：2018-05-22 00:00:00

abstract：:Corticotropin (ACTH) and melanotropin (MSH) peptides (melanocortins) are produced not only in the pituitary but also in the brain, with highest concentrations in the arcuate nucleus of the hypothalamus and the commisural nucleus of the solitary tract. We have identified a receptor for MSH and ACTH peptides that is spe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roselli-Rehfuss L,Mountjoy KG,Robbins LS,Mortrud MT,Low MJ,Tatro JB,Entwistle ML,Simerly RB,Cone RD

更新日期：1993-10-01 00:00:00

abstract：:X-ray diffraction studies have produced a low resolution image and also located the iron atoms of a monomeric hemerythrin from muscles of a sipunculan worm. These results reveal the course of the polypeptide chain and some details of the active center. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hendrickson WA,Klippenstein GL,Ward KB

更新日期：1975-06-01 00:00:00

abstract：:The basic theory of a newly discovered physical technique, steady-state electrophoresis, is described followed by analyses of several representative macro-ion systems with the technique. This method measures apparent moments of molar diminished charge (defined below) of solvated macro-ions from an analysis of a concen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Godfrey JE

更新日期：1989-06-01 00:00:00

abstract：:Environmental stress often leads to an increased production of reactive oxygen species that are involved in plastid-to-nucleus retrograde signaling. Soon after the release of singlet oxygen ((1)O(2)) in chloroplasts of the flu mutant of Arabidopsis, reprogramming of nuclear gene expression reveals a rapid transfer of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simková K,Moreau F,Pawlak P,Vriet C,Baruah A,Alexandre C,Hennig L,Apel K,Laloi C

更新日期：2012-10-02 00:00:00

abstract：:Thymic epithelial cells (TECs), the major component of the thymic microenvironment, can be modulated by pituitary hormones. We have shown previously that prolactin (PRL) can influence the endocrine activity of TECs and stimulate TEC proliferation as well as cytokeratin expression, suggesting the existence of PRL recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dardenne M,Kelly PA,Bach JF,Savino W

更新日期：1991-11-01 00:00:00

abstract：:We establish size limitations for assembling structures of controlled size and shape out of colloidal particles with short-ranged interactions. Through simulations we show that structures with highly variable shapes made out of dozens of particles can form with high yield, as long as each particle in the structure bin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeravcic Z,Manoharan VN,Brenner MP

更新日期：2014-11-11 00:00:00

abstract：:Protein-DNA interactions were studied in vivo at the region containing a human DNA replication origin, located at the 3' end of the lamin B2 gene and partially overlapping the promoter of another gene, located downstream. DNase I treatment of nuclei isolated from both exponentially growing and nonproliferating HL-60 c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dimitrova DS,Giacca M,Demarchi F,Biamonti G,Riva S,Falaschi A

更新日期：1996-02-20 00:00:00

abstract：:Adenosine deaminases that act on RNA (ADARs) constitute a family of RNA-editing enzymes that convert adenosine to inosine within double-stranded regions of RNA. We previously developed a method to identify inosine-containing RNAs and used it to identify five ADAR substrates in Caenorhabditis elegans. Here we use the s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morse DP,Aruscavage PJ,Bass BL

更新日期：2002-06-11 00:00:00

abstract：:The nuclear LIM domain protein LMO2, a T cell oncoprotein, is essential for embryonic erythropoiesis. LIM-only proteins are presumed to act primarily through protein-protein interactions. We, and others, have identified a widely expressed protein, Ldb1, whose C-terminal 76-residues are sufficient to mediate interactio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Visvader JE,Mao X,Fujiwara Y,Hahm K,Orkin SH

更新日期：1997-12-09 00:00:00

abstract：:Nitric oxide (NO) mediates several biological actions, including relaxation of blood vessels, cytotoxicity of activated macrophages, and formation of cGMP by activation of glutamate receptors in cerebellar slices. Nitric oxide synthase (EC 1.14.23.-) immunoreactivity is colocalized with nicotinamide adenine di-nucleot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dawson VL,Dawson TM,London ED,Bredt DS,Snyder SH

更新日期：1991-07-15 00:00:00

abstract：:Translocator protein (TSPO) is a key member of the mitochondrial cholesterol transport complex in steroidogenic tissues. To assess the function of TSPO, we generated two lines of Cre-mediated Tspo conditional knockout (cKO) mice. First, gonadal somatic cell-targeting Amhr2-Cre mice were crossed with Tspo-floxed mice t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan J,Campioli E,Midzak A,Culty M,Papadopoulos V

更新日期：2015-06-09 00:00:00

abstract：:The role of chemotaxis in the virulence of gastrointestinal pathogens is ill defined. Counterintuitively, nonchemotactic mutants of the polarly flagellated pathogen Vibrio cholerae greatly out-compete the wild-type strain during infection of the small intestine. We show that the out-competition phenotype is dependent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler SM,Camilli A

更新日期：2004-04-06 00:00:00

abstract：:An automatic protein design procedure is used to select amino acid sequences that optimize the folding free energy function for a given protein. The only information used in designing the sequences is a set of known backbone structures for each protein, a rotamer library, and a well established classical empirical for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaramillo A,Wernisch L,Héry S,Wodak SJ

更新日期：2002-10-15 00:00:00

abstract：:Eukaryotes engage in a multitude of beneficial and deleterious interactions with bacteria. Hamiltonella defensa, an endosymbiont of aphids and other sap-feeding insects, protects its aphid host from attack by parasitoid wasps. Thus H. defensa is only conditionally beneficial to hosts, unlike ancient nutritional symbio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Degnan PH,Yu Y,Sisneros N,Wing RA,Moran NA

更新日期：2009-06-02 00:00:00

abstract：:In feeding, aphids inject saliva into plant tissues, gaining access to phloem sap and eliciting (and sometimes overcoming) plant responses. We are examining the involvement, in this aphid-plant interaction, of individual aphid proteins and enzymes, as identified in a salivary gland cDNA library. Here, we focus on a sa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mutti NS,Louis J,Pappan LK,Pappan K,Begum K,Chen MS,Park Y,Dittmer N,Marshall J,Reese JC,Reeck GR

更新日期：2008-07-22 00:00:00