Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors.

abstract：:We have generated a hybridoma cell line secreting a monoclonal antibody that specifically binds to the surfaces of oligodendrocytes and Schwann cells, the cells involved in myelin formation in the central and peripheral nervous systems, respectively. Binding studies using purified sphingolipids showed that this antibo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ranscht B,Clapshaw PA,Price J,Noble M,Seifert W

更新日期：1982-04-01 00:00:00

abstract：:Listeria monocytogenes is an intracellular parasite that can readily infect the macrophage-like cell line J774 and the kidney epithelial cell PtK2. After being ingested, the organism escapes from the phagolysosome into the host-cell cytoplasm. N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-phallacidin, a specific stain for act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dabiri GA,Sanger JM,Portnoy DA,Southwick FS

更新日期：1990-08-01 00:00:00

abstract：:Cyclin-dependent kinase 4/6 (CDK4/6) inhibitors are an established treatment in estrogen receptor-positive breast cancer and are currently in clinical development in melanoma, a tumor that exhibits high rates of CDK4 activation. We analyzed melanoma cells with acquired resistance to the CDK4/6 inhibitor palbociclib an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： AbuHammad S,Cullinane C,Martin C,Bacolas Z,Ward T,Chen H,Slater A,Ardley K,Kirby L,Chan KT,Brajanovski N,Smith LK,Rao AD,Lelliott EJ,Kleinschmidt M,Vergara IA,Papenfuss AT,Lau P,Ghosh P,Haupt S,Haupt Y,Sanij E,

更新日期：2019-09-03 00:00:00

abstract：:The 1,25-dihydroxyvitamin D3 [1,25-(OH)2vitamin D3] analog KH1060 exerts very potent effects on cell proliferation and cell differentiation via the vitamin D receptor (VDR). However, the activities of KH1060 are not associated with an increased affinity for the VDR. We now show that increased stabilization of the VDR-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van den Bemd GC,Pols HA,Birkenhäger JC,van Leeuwen JP

更新日期：1996-10-01 00:00:00

abstract：:Two-dimensional 1H NMR investigations were used to locate elements of regular secondary structure in the human complement protein C3a (the des-Arg77 derivative) in solution. The results were compared to a refined crystal structure based on the 3.2-A resolution structure of des-Arg77-C3a [Huber, R., Scholze, H., Paques...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nettesheim DG,Edalji RP,Mollison KW,Greer J,Zuiderweg ER

更新日期：1988-07-01 00:00:00

abstract：:Toll-like receptor 3 (TLR3) is a key effector of the innate immune system against viruses. Activation of TLR3 exerts an antitumoral effect through a mechanism of action still poorly understood. Here we show that TLR3 activation by polyinosinic:polycytidylic acid induces up-regulation of microRNA-29b, -29c, -148b, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galli R,Paone A,Fabbri M,Zanesi N,Calore F,Cascione L,Acunzo M,Stoppacciaro A,Tubaro A,Lovat F,Gasparini P,Fadda P,Alder H,Volinia S,Filippini A,Ziparo E,Riccioli A,Croce CM

更新日期：2013-06-11 00:00:00

abstract：:Less than 100 cis-regulatory DNAs have been characterized in the context of transgenic metazoan embryos. Here we investigate the feasibility of conducting a genome-wide search for tissue-specific enhancers in the ascidian Ciona intestinalis. A total of 138 random genomic DNA fragments with an average size of 1.7 kb we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harafuji N,Keys DN,Levine M

更新日期：2002-05-14 00:00:00

abstract：:Herpes simplex virus 1 (HSV-1) specifies in the infected cell a set of polypeptides (alpha) whose mRNAs are made in the absence of protein synthesis. The individual mRNAs specifying alpha polypeptides 0, 4, 22, and 27 were purified by hybridization to strand-separated or total HSV-1 DNA fragments cloned in pBR322 plas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mackem S,Roizman B

更新日期：1980-12-01 00:00:00

abstract：:A decade ago, it seemed rational that our rapidly increasing knowledge of the molecular identities of tumor antigens and a deeper understanding of basic immunology would point the way to an effective therapeutic cancer vaccine. Significant progress has been made, but we do not yet have a cancer vaccine that can reliab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis JJ

更新日期：2004-10-05 00:00:00

abstract：:Understanding how institutional changes within academia may affect the overall potential of science requires a better quantitative representation of how careers evolve over time. Because knowledge spillovers, cumulative advantage, competition, and collaboration are distinctive features of the academic profession, both...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petersen AM,Riccaboni M,Stanley HE,Pammolli F

更新日期：2012-04-03 00:00:00

abstract：:Diabetes is now the most common cause of progressive kidney failure leading to dialysis or transplantation. The central pathological feature of diabetic nephropathy is accumulation of extracellular matrix within the glomeruli. The factors in the diabetic milieu responsible for extracellular matrix accumulation are not...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto T,Nakamura T,Noble NA,Ruoslahti E,Border WA

更新日期：1993-03-01 00:00:00

abstract：:Bacterial intracellular symbiosis (endosymbiosis) is widespread in nature and impacts many biological processes. In holometabolous symbiotic insects, metamorphosis entails a complete and abrupt internal reorganization that creates a constraint for endosymbiont transmission from larvae to adults. To assess how endosymb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maire J,Parisot N,Galvao Ferrarini M,Vallier A,Gillet B,Hughes S,Balmand S,Vincent-Monégat C,Zaidman-Rémy A,Heddi A

更新日期：2020-08-11 00:00:00

abstract：:The restoration of planar cell polarity is an essential but poorly understood step toward physiological recovery during sensory-organ regeneration. Investigating this issue in the lateral line of the zebrafish, we found that hair cells regenerate in pairs along a single axis established by the restricted localization ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： López-Schier H,Hudspeth AJ

更新日期：2006-12-05 00:00:00

abstract：:Of two closely related species of geese, one, the greylag goose, lives in the Indian plains all year round, while the other, the bar-headed goose, lives at the Tibetan lakes and migrates across the Himalayas to winter in India. Another species, the Andean goose, lives in the High Andes all year round. Possession of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jessen TH,Weber RE,Fermi G,Tame J,Braunitzer G

更新日期：1991-08-01 00:00:00

abstract：:Negative cooperativity has been observed in CTP synthetase, an allosteric enzyme which contains a regulatory site. Thus, the same enzyme exhibits negative cooperativity for GTP (an effector) and glutamine (a substrate) and positive cooperativity for ATP and UTP (both substrates). In the process of the delineation of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levitzki A,Koshland DE Jr

更新日期：1969-04-01 00:00:00

abstract：:Malignant B lymphocytes from several patients with chronic lymphocytic leukemia (CLL) were examined for reactivity with murine monoclonal antibody 17.109. This antibody, prepared against the rheumatoid factor (RF) paraprotein Sie, recognizes a crossreactive idiotype on 48% of human IgM RF paraproteins, but does not re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kipps TJ,Fong S,Tomhave E,Chen PP,Goldfien RD,Carson DA

更新日期：1987-05-01 00:00:00

abstract：:Tissue- and cell-specific deletion of the Aqp4 gene is required to differentiate between the numerous pools of aquaporin-4 (AQP4) water channels. A glial-conditional Aqp4 knockout mouse line was generated to resolve whether astroglial AQP4 controls water exchange across the blood-brain interface. The conditional knock...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haj-Yasein NN,Vindedal GF,Eilert-Olsen M,Gundersen GA,Skare Ø,Laake P,Klungland A,Thorén AE,Burkhardt JM,Ottersen OP,Nagelhus EA

更新日期：2011-10-25 00:00:00

abstract：:Evolutionary thinking can be applied to both cultural microevolution and macroevolution. However, much of the current literature focuses on cultural microevolution. In this article, we argue that the growing availability of large cross-cultural datasets facilitates the use of computational methods derived from evoluti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gray RD,Watts J

更新日期：2017-07-25 00:00:00

abstract：:The cellular actions of nerve growth factor (NGF) and epidermal growth factor (EGF) may be mediated by changes in protein phosphorylation. The tyrosine phosphorylation of two predominant proteins of molecular mass 40 and 42 kDa is seen in PC-12 cells treated with NGF or EGF, correlating with activation of a previously...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyasaka T,Sternberg DW,Miyasaka J,Sherline P,Saltiel AR

更新日期：1991-04-01 00:00:00

abstract：:Inactivating mutations of the pro-opiomelanocortin (POMC) gene in both mice and humans leads to hyperphagia and obesity. To further examine the mechanisms whereby POMC-deficiency leads to disordered energy homeostasis, we have generated mice lacking all POMC-derived peptides. Consistent with a previously reported mode...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Challis BG,Coll AP,Yeo GS,Pinnock SB,Dickson SL,Thresher RR,Dixon J,Zahn D,Rochford JJ,White A,Oliver RL,Millington G,Aparicio SA,Colledge WH,Russ AP,Carlton MB,O'Rahilly S

更新日期：2004-03-30 00:00:00

abstract：:FUS3 is functionally redundant with KSS1, a homologous yeast protein kinase, for a step(s) in signal transduction between the beta subunit of the guanine nucleotide binding protein (G protein), STE4, and the mating type-specific transcriptional activator, STE12. Either FUS3 or KSS1 can execute this function; when neit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elion EA,Brill JA,Fink GR

更新日期：1991-11-01 00:00:00

abstract：:The redox potentials of electron transfer proteins vary over a wide range, even when the type of redox center is the same. Rees [Proc. Natl. Acad. Sci. USA (1985) 82, 3082-3085] proposed that this variation of redox potential partly reflects the different net charges of the proteins, and he presented a linear correlat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore GR,Pettigrew GW,Rogers NK

更新日期：1986-07-01 00:00:00

abstract：:Society makes substantial investments in biomedical research, searching for ways to better human health. The product of this research is principally information published in scientific journals. Continued investment in science relies on society's confidence in the accuracy, honesty, and utility of research results. A ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowen A,Casadevall A

更新日期：2015-09-08 00:00:00

abstract：:Colonization of land by plants was a major transition on Earth, but the developmental and genetic innovations required for this transition remain unknown. Physiological studies and the fossil record strongly suggest that the ability of the first land plants to form symbiotic associations with beneficial fungi was one ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Delaux PM,Radhakrishnan GV,Jayaraman D,Cheema J,Malbreil M,Volkening JD,Sekimoto H,Nishiyama T,Melkonian M,Pokorny L,Rothfels CJ,Sederoff HW,Stevenson DW,Surek B,Zhang Y,Sussman MR,Dunand C,Morris RJ,Roux C,Wong GK

更新日期：2015-10-27 00:00:00

abstract：:Recombinant adenoviruses containing the canine factor IX (FIX) cDNA were directly introduced in the hind leg muscle of mice. We show that (i) in nude mice, high expression (1-5 micrograms/ml in plasma) of FIX protein can be detected for > 300 days; (ii) in contrast, expression of FIX protein was transient (7-10 days) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai Y,Schwarz EM,Gu D,Zhang WW,Sarvetnick N,Verma IM

更新日期：1995-02-28 00:00:00

abstract：:Many ion channels exhibit a slow stochastic switching between distinct modes of gating activity. This feature of channel behavior has pronounced implications for the dynamics of ionic currents and the signaling pathways that they regulate. A canonical example is the inositol 1,4,5-trisphosphate receptor (IP3R) channel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bicknell BA,Goodhill GJ

更新日期：2016-09-06 00:00:00

abstract：:This work argues that, in a polarized environment, scientists can minimize the likelihood that the audience's biased processing will lead to rejection of their message if they not only eschew advocacy but also, convey that they are sharers of knowledge faithful to science's way of knowing and respectful of the audienc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamieson KH,Hardy BW

更新日期：2014-09-16 00:00:00

abstract：:The nonlinearity of the Navier-Stokes equations makes predicting the flow of fluid around rapidly moving small bodies highly resistant to all approaches save careful experiments or brute force computation. Here, we show how a linearization of the Navier-Stokes equations captures the drag-determining features of the fl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roper M,Brenner MP

更新日期：2009-03-03 00:00:00

abstract：:Cytokines such as interleukin 4 (IL-4) play a key role in the regulation of immune responses, but little is known about how their multiple activities are regulated in vivo. In this report, we demonstrated that an IL-4-binding protein (IL-4BP) is constitutively present in the biological fluids of mice (serum, ascites, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandez-Botran R,Vitetta ES

更新日期：1990-06-01 00:00:00

abstract：:We have established a recombinant inbred strain of mouse named spontaneous crescentic glomerulonephritis-forming mouse/Kinjoh or SCG/Kj. Mice of this strain spontaneously develop rapidly progressive glomerulonephritis. This strain of mice was derived from (BXSB/Mp x MRL/Mp-lpr/lpr)F1 hybrid mice by brother x sister ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kinjoh K,Kyogoku M,Good RA

更新日期：1993-04-15 00:00:00