FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction.

abstract：:Biotin carboxylase [biotin-carboxyl-carrier-protein:carbon-dioxide ligase (ADP-forming), EC 6.3.4.14] is the enzyme mediating the first step of the acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] reaction. We screened an Escherichia coli DNA library and a DNA fragment carrying the b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo H,Shiratsuchi K,Yoshimoto T,Masuda T,Kitazono A,Tsuru D,Anai M,Sekiguchi M,Tanabe T

更新日期：1991-11-01 00:00:00

abstract：:An artificial increase of cyclic diguanylate (c-di-GMP) levels in Sinorhizobium meliloti 8530, a bacterium that does not carry known cellulose synthesis genes, leads to overproduction of a substance that binds the dyes Congo red and calcofluor. Sugar composition and methylation analyses and NMR studies identified this...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pérez-Mendoza D,Rodríguez-Carvajal MÁ,Romero-Jiménez L,Farias Gde A,Lloret J,Gallegos MT,Sanjuán J

更新日期：2015-02-17 00:00:00

abstract：:Estrogen signaling occurs through at least two distinct molecular pathways: (i) direct binding of liganded estrogen receptors (ERs) to estrogen-responsive DNA elements (EREs) (the "ER/ERE pathway") and (ii) indirect recruitment of liganded ERs to activating protein-1 (AP-1)-responsive DNA elements via heterodimers of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung E,Acevedo ML,Cole PA,Kraus WL

更新日期：2005-01-18 00:00:00

abstract：:Effects of temperature on biological processes are complex. Diffusion is less affected than the diverse enzymatic reactions that have distinct individual temperature profiles. Hence thermal fluctuations pose a formidable challenge to ectothermic organisms in which body temperature is largely dictated by the ambient te...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Radermacher PT,Myachina F,Bosshardt F,Pandey R,Mariappa D,Müller HA,Lehner CF

更新日期：2014-04-15 00:00:00

abstract：:Efficient 3'-end processing of cell cycle-regulated mammalian histone premessenger RNAs (pre-mRNAs) requires an upstream stem-loop and a histone downstream element (HDE) that base pairs with the U7 small ribonucleoprotein. Insertions between these elements have two effects: the site of cleavage moves in concert with t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scharl EC,Steitz JA

更新日期：1996-12-10 00:00:00

abstract：:Three genome-wide RNA interference screens were performed in Drosophila S2 cells to dissect the contribution of host processes to Listeria monocytogenes entry, vacuolar escape, and intracellular growth. Among the 116 genes identified, several host pathways previously unrecognized as playing a role in listerial pathoge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng LW,Viala JP,Stuurman N,Wiedemann U,Vale RD,Portnoy DA

更新日期：2005-09-20 00:00:00

abstract：:Two major problems have to be solved in studies of genes near breakpoints of chromosome abnormalities and in large-scale genomic mapping projects: (i) the identification of genes within the large amount on nontranscribed DNA and (ii) the determination of the tissues in which the identified genes are transcribed. In si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boehm T,Gonzalez-Sarmiento R,Kennedy M,Rabbitts TH

更新日期：1991-05-01 00:00:00

abstract：:Heparanase is an endoglycosidase that cleaves heparan sulfate side chains of proteoglycans, resulting in disassembly of the extracellular matrix underlying endothelial and epithelial cells and associating with enhanced cell invasion and metastasis. Heparanase expression is induced in carcinomas and sarcomas, often ass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weissmann M,Arvatz G,Horowitz N,Feld S,Naroditsky I,Zhang Y,Ng M,Hammond E,Nevo E,Vlodavsky I,Ilan N

更新日期：2016-01-19 00:00:00

abstract：:Reestablishment of vascular homeostasis following ex vivo preservation is a critical determinant of successful organ transplantation. Because the nitric oxide (NO) pathway modulates pulmonary vascular tone and leukocyte/endothelial interactions, we hypothesized that reactive oxygen intermediates would lead to decrease...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pinsky DJ,Naka Y,Chowdhury NC,Liao H,Oz MC,Michler RE,Kubaszewski E,Malinski T,Stern DM

更新日期：1994-12-06 00:00:00

abstract：:In the absence of any overt task performance, it has been shown that spontaneous, intrinsic brain activity is expressed as systemwide, resting-state networks in the adult brain. However, the route to adult patterns of resting-state activity through neuronal development in the human brain is currently unknown. Therefor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fransson P,Skiöld B,Horsch S,Nordell A,Blennow M,Lagercrantz H,Aden U

更新日期：2007-09-25 00:00:00

abstract：:Calmodulin (CaM) is a second messenger protein that has evolved to bind tightly to a variety of targets and, as such, exhibits low binding specificity. We redesigned CaM by using a computational protein design algorithm to improve its binding specificity for one of its targets, smooth muscle myosin light chain kinase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shifman JM,Mayo SL

更新日期：2003-11-11 00:00:00

abstract：:We introduce Pathifier, an algorithm that infers pathway deregulation scores for each tumor sample on the basis of expression data. This score is determined, in a context-specific manner, for every particular dataset and type of cancer that is being investigated. The algorithm transforms gene-level information into pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drier Y,Sheffer M,Domany E

更新日期：2013-04-16 00:00:00

abstract：:The homogeneous exchange process whereby IgG molecules adsorbed onto latex particles are replaced by IgG molecules from the bulk solution was studied by means of 125I radiolabeling. The exchange mechanism was investigated on surfaces saturated with either labeled or unlabeled proteins in the presence of a solution of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ball V,Huetz P,Elaissari A,Cazenave JP,Voegel JC,Schaaf P

更新日期：1994-07-19 00:00:00

abstract：:The B cell developmental pathway represents a leading system for the analysis of regulatory circuits that orchestrate cell fate specification and commitment. Considerable progress has been achieved within the past decade in the identification and genetic analysis of various regulatory components. These components incl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh H,Medina KL,Pongubala JM

更新日期：2005-04-05 00:00:00

abstract：:The functional role of residue Tyr-19 of Chromatium vinosum HiPIP has been evaluated by site-directed mutagenesis experiments. The stability of the [Fe4S4] cluster prosthetic center is sensitive to side-chain replacements. Polar residues result in significant instability, while nonpolar residues (especially with aroma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agarwal A,Li D,Cowan JA

更新日期：1995-10-10 00:00:00

abstract：:In suspension cultures of mouse spleen cells, the addition of small amounts (1-100 ng) of a heterologous antigen to which the donor mice had been primed significantly increased the number of anti-sheep erythrocyte plaques appearing after stimulation with sheep erythrocytes. A similar addition to identical cultures fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rubin AS,Coons AH

更新日期：1971-08-01 00:00:00

abstract：:Ribonuclease H (EC 3.1.4.34) has been used to remove selectively much of the 3'-terminal poly(adenylic acid) [poly(A)] from poliovirus RNA by treating the RNA with the enzyme in the presence of poly(dT). Over 80% of the poly(A) could be removed and the residuum was found as oligo(A) stretches attached to many or all o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spector DH,Baltimore D

更新日期：1974-08-01 00:00:00

abstract：:Trace greenhouse gases are a fundamentally important component of Earth's global climate system sensitive to global change. However, their concentration in the pre-Pleistocene atmosphere during past warm greenhouse climates is highly uncertain because we lack suitable geochemical or biological proxies. This long-stand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beerling DJ,Fox A,Stevenson DS,Valdes PJ

更新日期：2011-06-14 00:00:00

abstract：:In the presence but not in the absence of pokeweed mitogen (PWM), monoclonal antibodies against HLA-DR antigens 147 and 164 helped highly purified B lymphocytes to proliferate and mature to Ig-secreting cells. In contrast, neither anti-DR antibody 231 nor the UCHT1 monoclonal anti-human T cell antibody (both of the sa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palacios R,Martinez-Maza O,Guy K

更新日期：1983-06-01 00:00:00

abstract：:Phospholamban (PLN) is an effective inhibitor of the sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA). Here, we examined PLN stability and degradation in primary cultured mouse neonatal cardiomyocytes (CMNCs) and mouse hearts using immunoblotting, molecular imaging, and [(35)S]methionine pulse-chase experiments, tog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teng AC,Miyake T,Yokoe S,Zhang L,Rezende LM Jr,Sharma P,MacLennan DH,Liu PP,Gramolini AO

更新日期：2015-06-09 00:00:00

abstract：:We isolated mutant strains of the facultative anaerobe Salmonella typhimurium that grow either aerobically or anaerobically. Strict anaerobic mutants contained a defective DNA topoisomerase I gene (topI), while strict aerobic mutants contained a defective DNA gyrase subunit A gene (gyrA, also nalA). Topoisomerase I ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto N,Droffner ML

更新日期：1985-04-01 00:00:00

abstract：:Rat liver glutamine synthetase (s(20,w) 15.0 S; MW about 352,000) resembles ovine brain glutamine synthetase in that it (a) has 8 subunits, (b) acts on both L- and D-glutamate and certain glutamate analogs (e.g., beta-glutamate, cis-cycloglutamate, and alpha-methyl-L-glutamate), and (c) is irreversibly inhibited by L-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tate SS,Meister A

更新日期：1971-04-01 00:00:00

abstract：:The MYC family of cellular oncogenes includes c-Myc, N-myc, and L-myc, which encode transcriptional regulators involved in the control of cell proliferation and death. Accordingly, these genes become aberrantly activated and expressed in specific types of cancers. For example, c-Myc translocations occur frequently in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gostissa M,Ranganath S,Bianco JM,Alt FW

更新日期：2009-02-17 00:00:00

abstract：:In considering the structure of the 30S subunit of the Escherichia coli ribosome, we have assumed that: (i) all or almost all the proteins within the 30S particle are compact and globular, as recently shown for the isolated proteins S4, S7, S8, S15, and S16 in solution [Serdyuk, I.N., Zaccai, G. & Spirin, A.S. (1978) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spirin AS,Serdyuk IN,Shpungin JL,Vasiliev VD

更新日期：1979-10-01 00:00:00

abstract：:Figure-ground discrimination refers to the perception of an object, the figure, against a nondescript background. Neural mechanisms of figure-ground detection have been associated with feedback interactions between higher centers and primary visual cortex and have been held to index the effect of global analysis on lo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones HE,Andolina IM,Shipp SD,Adams DL,Cudeiro J,Salt TE,Sillito AM

更新日期：2015-06-02 00:00:00

abstract：:In eukaryotic cells, RAD52 protein plays a central role in genetic recombination and DNA repair by (i) promoting the annealing of complementary single-stranded DNA and (ii) stimulation of the RAD51 recombinase. The single-strand annealing domain resides in the N-terminal region of the protein and is highly conserved, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singleton MR,Wentzell LM,Liu Y,West SC,Wigley DB

更新日期：2002-10-15 00:00:00

abstract：:Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun N,Panetta NJ,Gupta DM,Wilson KD,Lee A,Jia F,Hu S,Cherry AM,Robbins RC,Longaker MT,Wu JC

更新日期：2009-09-15 00:00:00

abstract：:Recent work with prion diseases and synucleinopathies indicates that accurate diagnostic methods for protein-folding diseases can be based on the ultrasensitive, amplified measurement of pathological aggregates in biospecimens. A better understanding of the physicochemical factors that control the seeded polymerizatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Metrick MA 2nd,do Carmo Ferreira N,Saijo E,Hughson AG,Kraus A,Orrú C,Miller MW,Zanusso G,Ghetti B,Vendruscolo M,Caughey B

更新日期：2019-11-12 00:00:00

abstract：:As sensors, wireless communication devices, personal health monitoring systems, and autonomous microelectromechanical systems (MEMS) become distributed and smaller, there is an increasing demand for miniaturized integrated power sources. Although thin-film batteries are well-suited for on-chip integration, their energ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ning H,Pikul JH,Zhang R,Li X,Xu S,Wang J,Rogers JA,King WP,Braun PV

更新日期：2015-05-26 00:00:00

abstract：:Lithium-air batteries have captured worldwide attention due to their highest energy density among the chemical batteries. To provide continuous oxygen channels, here, we synthesized hierarchical mesoporous perovskite La(0.5)Sr(0.5)CoO(2.91) (LSCO) nanowires. We tested the intrinsic oxygen reduction reaction (ORR) and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Y,Xu L,Mai L,Han C,An Q,Xu X,Liu X,Zhang Q

更新日期：2012-11-27 00:00:00