Abstract:
:Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients. Furthermore, iPS cells can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells. hASCs can be safely and readily isolated from adult humans in large quantities without extended time for expansion, are easy to maintain in culture, and therefore represent an ideal autologous source of cells for generating individual-specific iPS cells.
journal_name
Proc Natl Acad Sci U S Aauthors
Sun N,Panetta NJ,Gupta DM,Wilson KD,Lee A,Jia F,Hu S,Cherry AM,Robbins RC,Longaker MT,Wu JCdoi
10.1073/pnas.0908450106subject
Has Abstractpub_date
2009-09-15 00:00:00pages
15720-5issue
37eissn
0027-8424issn
1091-6490pii
0908450106journal_volume
106pub_type
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