Abstract:
:Serotonin (5-HT) plays a critical role in modulating synaptic plasticity in the marine mollusc Aplysia and in the mammalian nervous system. In Aplysia sensory neurons, 5-HT can activate several signal cascades, including PKA and PKC, presumably via distinct types of G protein-coupled receptors. However, the molecular identities of these receptors have not yet been identified. We here report the cloning and functional characterization of a 5-HT receptor that is positively coupled to adenylyl cyclase in Aplysia neurons. The cloned receptor, 5-HT(apAC1), stimulates the production of cAMP in HEK293T cells and in Xenopus oocytes. Moreover, the knockdown of 5-HT(apAC1) expression by RNA interference blocked 5-HT-induced cAMP production in Aplysia sensory neurons and blocked synaptic facilitation in nondepressed or partially depressed sensory-to-motor neuron synapses. These data implicate 5-HT(apAC1) as a major modulator of learning related synaptic facilitation in the direct sensory to motor neuron pathway of the gill withdrawal reflex.
journal_name
Proc Natl Acad Sci U S Aauthors
Lee YS,Choi SL,Lee SH,Kim H,Park H,Lee N,Lee SH,Chae YS,Jang DJ,Kandel ER,Kaang BKdoi
10.1073/pnas.0907502106subject
Has Abstractpub_date
2009-08-25 00:00:00pages
14634-9issue
34eissn
0027-8424issn
1091-6490pii
0907502106journal_volume
106pub_type
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