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Protein-loaded PLGA microparticles engineered by flow focusing: physicochemical characterization and protein detection by reversed-phase HPLC.

Abstract：

:In the present study, a novel synthesis technique based on the flow focusing (FF) technology is investigated for the preparation of green fluorescent protein (GFP)-loaded poly(D,L-lactide-co-glycolide) (PLGA) microparticles. To our knowledge, this novel technique has never been applied to the formulation of proteins in polymeric systems. A simple, specific and rapid reversed-phase HPLC (RP-HPLC) method was validated for the determination of GFP in PLGA microparticles with the best chromatographic peak resolution, reduced run time and low cost of analysis. In order to achieve the finest GFP-loaded polymeric particles, experimental parameters mainly associated to the FF device were studied (liquid flow rate and pressure of the focusing air). Very high GFP encapsulation values (>90%) were obtained by this technique, and the electrokinetic characterization of these systems suggested that this protein was incorporated into the polymeric matrix. This study is intended to offer information on which to base the development of high molecular weight protein-loaded polymeric delivery systems prepared by FF.

journal_name

Int J Pharm

journal_title

International journal of pharmaceutics

authors

Holgado MA,Cózar-Bernal MJ,Salas S,Arias JL,Alvarez-Fuentes J,Fernández-Arévalo M

doi

10.1016/j.ijpharm.2009.07.017

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

147-54

issue

1-2

eissn

0378-5173

issn

1873-3476

pii

S0378-5173(09)00491-8

journal_volume

380

pub_type

杂志文章

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