Abstract：
:In order to develop a mucosal delivery system based on biocompatible polymers, a new methodology for production of protein-loaded microparticles is developed. Chitosan anionic precipitation/coacervation is accomplished by the addition of sodium deoxycholate (DCA). These microparticles were prepared under mild conditions, where bovine serum albumin (BSA) and DCA were simply dipped into a chitosan solution under stirring. Platelet-like and/or spherical microparticles, having high protein loading efficiency and relatively low protein external exposure, are obtained. To achieve a better compaction of the microparticle matrix, block copolymers and other non-ionic surfactants are added to the formulation. BCA analysis and fluorescence quenching were used to assess the degree of protein exposure. BSA release profiles for chitosan-DCA formulations in PBS pH 7.4 and HCl 0.1 N revealed, in most cases, an initial burst release, but more than 55% of the BSA remains protected inside the microparticles. It is also observed that in acidic environment (HCl 0.1 N) the protein is better shielded from the environment. Some of the formulations show good properties for mucosal protein delivery, and one of those here developed is now being tested in vivo, for mucosal administration of an adenovirus vaccine.
journal_name
Int J Pharmjournal_title
International journal of pharmaceuticsauthors
Lameiro MH,Lopes A,Martins LO,Alves PM,Melo Edoi
10.1016/j.ijpharm.2006.01.006keywords:
subject
Has Abstractpub_date
2006-04-07 00:00:00pages
119-30issue
1-2eissn
0378-5173issn
1873-3476pii
S0378-5173(06)00034-2journal_volume
312pub_type
杂志文章abstract：:Nanoparticles comprising copolymers of aminoethylcarbamoyl-beta-cyclodextrin (AEC-beta-CD) and ethylene glycol diglycidyl ether (EGDGE) are prepared by an interfacial polyaddition reaction in a miniemulsion system. Polymers are formed in a W/O emulsion containing 0.25-10.0% (w/w) water and 5.0% (w/w) surfactant (MO-3S...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2006-03-27 00:00:00
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2010-09-15 00:00:00
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abstract：:Poly(ethylene glycol)-conjugated polyethylenimine (PEG-PEI) is a widely studied cationic polymer used to develop non-viral vectors for siRNA therapy of genetic disorders including cancer. Cell lines stably expressing luciferase reporter protein typically evaluate the transfection efficacy of siRNA/PEG-PEI complexes, h...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
pub_type： 杂志文章,评审
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journal_title：International journal of pharmaceutics
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journal_title：International journal of pharmaceutics
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更新日期：2008-04-16 00:00:00
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pub_type： 杂志文章
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journal_title：International journal of pharmaceutics
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更新日期：2016-04-30 00:00:00
abstract：:Antibiotics are well-known disruptive elements of the intestinal microbiota and antibiotic-associated diarrhea appeared as the most common complication related with post-antibiotic dysbiosis. Lactobacillus rhamnosus GG (LGG) strain is very effective in preventing antibiotic-associated diarrhea in children and adults. ...
journal_title：International journal of pharmaceutics
pub_type： 杂志文章
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更新日期：2020-06-30 00:00:00
