Abstract:
BACKGROUND:Evidence supports the role of adherent invasive Escherichia coli (AIEC) in the pathogenesis of inflammatory bowel disease (IBD). However, little is known about the phylogenetic structure and origin of this group of bacteria. Multi-locus sequence typing (MLST), and fimH sequence analysis were performed to elucidate the phylogenetic relationships between E. coli strains isolated from IBD tissue. METHODS:Thirty-six E. coli isolated from IBD patients and healthy individuals were used. MLST analysis of the adk, fumC, gyrB, icd, mdh, purA, and recA housekeeping genes was performed. The fimH gene was also sequenced and phylogenetically analyzed. Biochemical profiling of strains were performed using the API 20 E system. RESULTS:MLST analysis distinguished 9 new alleles and 11 new sequence types, nearly all of which belonged to IBD isolates. E. coli isolated from IBD patients were more likely to be grouped into separate clonal clusters by eBURST analysis of allelic profiles (P = 0.02). Sequencing of fimH placed putative AIEC strains into the same cluster with the uro-pathogenic E. coli CFT073 and the avian-pathogenic E. coli O1:K1:H7. CONCLUSIONS:MLST analysis suggested that E. coli isolated from IBD patients did not evolve from a unique ancestral background. Together with the fimH sequence we conclude that AIEC represent a group of bacteria that have been able to take advantage of an "IBD microenvironment" and likely shares common genes with extraintestinal pathogens like uro-pathogenic CFT073 and avian-pathogenic O1:K1:H7 E. coli. Future research should focus on genes that are unique to AIEC.
journal_name
Inflamm Bowel Disjournal_title
Inflammatory bowel diseasesauthors
Sepehri S,Kotlowski R,Bernstein CN,Krause DOdoi
10.1002/ibd.20966subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1737-45issue
11eissn
1078-0998issn
1536-4844journal_volume
15pub_type
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journal_title:Inflammatory bowel diseases
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,随机对照试验
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journal_title:Inflammatory bowel diseases
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doi:10.1093/ibd/izaa183
更新日期:2020-09-18 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,多中心研究
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abstract:BACKGROUND:The incidence of inflammatory bowel disease (IBD) has increased over the past several decades with a corresponding increase in the number of patients on combination immunosuppressive therapy including corticosteroids, anti-metabolites and biologic agents. The exact incidence of pneumocystis jiroveci pneumoni...
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更新日期:2013-07-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1002/ibd.21241
更新日期:2010-08-01 00:00:00
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journal_title:Inflammatory bowel diseases
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journal_title:Inflammatory bowel diseases
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journal_title:Inflammatory bowel diseases
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1002/ibd.21113
更新日期:2010-04-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
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更新日期:2018-02-15 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1002/ibd.20042
更新日期:2007-04-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1093/ibd/izy182
更新日期:2018-08-16 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1097/MIB.0000000000000871
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:The incidence of very early onset inflammatory bowel disease (VEOIBD) is increasing, yet the phenotype and natural history of VEOIBD are not well described. METHODS:We performed a retrospective cohort study of patients diagnosed with VEOIBD (6 years of age and younger) between 2008 and 2013 at 25 North Amer...
journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1093/ibd/izaa080
更新日期:2020-05-09 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1093/ibd/izz282
更新日期:2020-10-23 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1002/ibd.20774
更新日期:2009-03-01 00:00:00
abstract::Diagnostics of inflammatory bowel diseases (IBDs) currently relies on a combination of biological and morphological tests. The current method of diagnostic remains a critical challenge for physicians in part due to their invasiveness and also for their limitations in term of diagnosis, prognosis, disease activity and ...
journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
doi:10.1002/ibd.20797
更新日期:2009-06-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,随机对照试验
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更新日期:2011-09-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1097/MIB.0000000000000699
更新日期:2016-04-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1002/ibd.20750
更新日期:2009-02-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1002/ibd.21152
更新日期:2010-06-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,多中心研究
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更新日期:2017-12-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,收录出版
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更新日期:2010-05-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 临床试验,杂志文章
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更新日期:2017-08-01 00:00:00