当前位置： SCI文献检索 > INFLAMMATORY BOWEL DISEASES期刊下所有文献 > Increased Proliferation of the Ileal Epithelium as a Remote Effect of Ulcerative Colitis.

Increased Proliferation of the Ileal Epithelium as a Remote Effect of Ulcerative Colitis.

Abstract：

BACKGROUND:Aside from cases of backwash ileitis, the ileal mucosa of patients with ulcerative colitis (UC), an idiotypic inflammatory bowel disease, has received little attention despite the fact that colitis is known to trigger alterations in morphology and/or functions of the small intestine remotely. METHODS:The ileal mucosa was studied in patients with UC and in a spontaneous model of colitis (Il10/Nox1 mice) mimicking the histological and clinical features of UC and was also studied in acute and chronic murine models of chemically induced colitis. Proliferation and apoptosis were assessed using morphological and immunohistological methods and Western blot analysis. Peyer's patch immune cell subsets were analyzed. Cytokines levels were quantified using quantitative PCR and Luminex xMAP technology. Total RNA from isolated ileal crypts was used for whole genome transcriptome analysis. RESULTS:The most striking features were an increased ileal crypt length associated with an enhanced cell proliferation of the transit-amplifying cells along with activation of the Wnt/β-catenin and MAPkinase pathways. These changes did not result from intestinal inflammation as assessed by histology and/or pro-inflammatory cytokine expression levels. The increased proliferation rate was dependent on the duration but not on the severity of colitis and was observed in different mouse models of colitis, including the Il10/Nox1 model and 2,4,6-trinitrobenzenesulfonic acid-treated mice. Interestingly, the ileal mucosa of patients with UC also displayed longer crypts and enhanced cell proliferation compared with control patients. CONCLUSIONS:These data show that despite the absence of inflammation in the small intestine, alterations in the ileal mucosa homeostasis are present in UC.

journal_name

Inflamm Bowel Dis

journal_title

Inflammatory bowel diseases

authors

Sedghi S,Barreau F,Morilla I,Montcuquet N,Cazals-Hatem D,Pedruzzi E,Rannou E,Tréton X,Hugot JP,Ogier-Denis E,Daniel F

doi

10.1097/MIB.0000000000000871

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

2369-81

issue

eissn

1078-0998

issn

1536-4844

journal_volume

pub_type

杂志文章

在线工具

Increased Proliferation of the Ileal Epithelium as a Remote Effect of Ulcerative Colitis.