Abstract:
BACKGROUND:Mucosa-associated Escherichia coli are abundant in inflammatory bowel disease (IBD), but whether these bacteria gain intracellular access within the mucosa is uncertain. If E. coli does gain intracellular access, the contribution of bacterial pathogenicity to this requires further elucidation. This study aimed to quantify and characterize mucosa-associated and intracellular E. coli in patients with IBD and in healthy control subjects (HC). METHODS:Mucosal biopsies from 30 patients with Crohn's disease (CD), 15 with ulcerative colitis (UC), and 14 HC were cultured with or without gentamicin protection to recover intracellular or mucosa-associated E. coli, respectively. Overall, 40 strains (CD: n = 24, UC: n = 9, and HC: n = 7) were characterized by phylogenetic typing, adhesion and invasion assays, detection of virulence factors, antimicrobial resistance genes, and proteomic analysis. RESULTS:Mucosa-associated E. coli were more abundant in CD and UC than in HC (2750 versus 1350 versus 230 median colony-forming units per biopsy; P = 0.01). Intracellular E. coli were more prevalent in CD (90%) than in UC (47%) or HC mucosal biopsies (0%) (P < 0.001). Of 24 CD strains, 2 were adherent and invasive, but there were no unifying pathogenicity determinants that could distinguish most CD strains from UC or HC strains, or intracellular isolates from mucosa-associated isolates. CONCLUSIONS:Intracellular E. coli are more common in CD than in UC and not identified in HC. Most intracellular E. coli did not have characterizing pathogenic features, suggesting a significant role for defects in mucosal immunity or barrier dysfunction in their ability to gain intracellular access.
journal_name
Inflamm Bowel Disjournal_title
Inflammatory bowel diseasesauthors
Elliott TR,Hudspith BN,Wu G,Cooley M,Parkes G,Quiñones B,Randall L,Mandrell RE,Fagerquist CK,Brostoff J,Rayment NB,Boussioutas A,Petrovska L,Sanderson JDdoi
10.1097/MIB.0b013e3182a38a92subject
Has Abstractpub_date
2013-10-01 00:00:00pages
2326-38issue
11eissn
1078-0998issn
1536-4844journal_volume
19pub_type
杂志文章abstract::This review summarizes the current phenotypic classifications of inflammatory bowel disease (IBD) and outlines their implications for diagnosis, therapy, prognosis, clinical trial design, and genotype-phenotype correlations. ...
journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1002/ibd.20232
更新日期:2007-12-01 00:00:00
abstract:BACKGROUND:Fibrostenosis and stricture are well-recognized endpoints in Crohn's disease (CD). We hypothesized that stricturing CD is characterized by eosinophilia and epithelial IL-33. We proposed that eosinophil exposure to IL-33 would perpetuate inflammatory chronicity and subsequent fibrostenosis. METHODS:We perfor...
journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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pub_type: 杂志文章,多中心研究
doi:10.1097/MIB.0000000000000413
更新日期:2015-07-01 00:00:00
abstract::Biologic treatments have revolutionized the way we treat inflammatory bowel disease patients (IBD). Anti-tumor necrosis factor (anti-TNF) antibodies are superior to conventional therapies to achieve sustained remission without steroids and mucosal healing. The objective of IBD treatment has evolved from symptom allevi...
journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1097/01.mib.0000235830.94057.c6
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND:High doses of mesalazine usually result in an inconvenient dosage schedule and reduced compliance. The goal of this trial was to compare the effects of mesalazine 4 g daily given as prolonged-release granules in packets of 1 g with that of prolonged-release tablets of 0.5 g. METHODS:Two hundred twenty-seven...
journal_title:Inflammatory bowel diseases
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00054725-200108000-00009
更新日期:2001-08-01 00:00:00
abstract:BACKGROUND:The majority of studies concerning the clinical course and prognosis in ulcerative colitis (UC) are old, retrospective in design, or hospital based. We aimed to identify clinical course and prognosis in a prospective, population-based follow-up study MATERIALS AND METHODS:Patients diagnosed with inflammator...
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doi:10.1097/01.MIB.0000225339.91484.fc
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,多中心研究
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更新日期:2009-08-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
doi:10.1002/ibd.20239
更新日期:2007-12-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1097/MIB.0000000000000939
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
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更新日期:2014-08-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
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更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/ibd.20774
更新日期:2009-03-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1097/MIB.0000000000000322
更新日期:2015-04-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
doi:10.1097/00054725-200209000-00006
更新日期:2002-09-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1093/ibd/izz281
更新日期:2020-03-04 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
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更新日期:2008-07-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2013-07-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章,评审
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更新日期:2011-11-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:10.1097/01.mib.0000195390.11645.7d
更新日期:2006-01-01 00:00:00
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journal_title:Inflammatory bowel diseases
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-10-01 00:00:00
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pub_type: 杂志文章,meta分析
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更新日期:2018-04-23 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/ibd.20265
更新日期:2007-12-01 00:00:00