Identification of B-cell epitopes of Bet v 1 involved in cross-reactivity with food allergens.

Abstract:

BACKGROUND:The pollen-food syndrome (PFS) is an association of food allergies to fruits, nuts, and vegetables in patients with pollen allergy. Mal d 1, the major apple allergen, is one of the most commonly associated food allergens for birch pollen-allergic patients suffering from PFS. Although the reactions are due to cross-reactive IgE antibodies originally raised against pollen Bet v 1, not every Bet v 1-allergic patient develops clinical reactions towards apple. AIM OF THE STUDY:We speculate that distinct IgE epitopes are responsible for the clinical manifestation of PFS. To test this hypothesis we grafted five Mal d 1 stretches onto Bet v 1. The grafted regions were 7- or 8-amino acids long encompassing amino acids residues previously shown to be crucial for IgE recognition of Bet v 1. METHODS:A Bet v 1-Mal d 1 chimeric protein designated BMC was expressed in Escherichia coli and purified to homogeneity. IgE reactivity of BMC was tested with patients' sera originating from (i) Bet v 1-allergic patients displaying no clinical symptoms upon ingestion of apples; and (ii) Bet v 1-allergic patients displaying allergic symptoms upon ingestion of apples and other Bet v 1-related foods. RESULTS AND CONCLUSION:Compared to birch pollen-allergic individuals, patients suffering from PFS showed significantly higher IgE reactivity with BMC (chimeric protein). The results suggest that the Mal d 1 regions grafted onto the Bet v 1 sequence comprise important IgE epitopes recognized by Bet v 1-allergic patients suffering from allergy to apples.

journal_name

Allergy

journal_title

Allergy

authors

Klinglmayr E,Hauser M,Zimmermann F,Dissertori O,Lackner P,Wopfner N,Ferreira F,Wallner M

doi

10.1111/j.1398-9995.2008.01844.x

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

647-51

issue

4

eissn

0105-4538

issn

1398-9995

pii

ALL1844

journal_volume

64

pub_type

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