Identification of B-cell epitopes of Bet v 1 involved in cross-reactivity with food allergens.


BACKGROUND:The pollen-food syndrome (PFS) is an association of food allergies to fruits, nuts, and vegetables in patients with pollen allergy. Mal d 1, the major apple allergen, is one of the most commonly associated food allergens for birch pollen-allergic patients suffering from PFS. Although the reactions are due to cross-reactive IgE antibodies originally raised against pollen Bet v 1, not every Bet v 1-allergic patient develops clinical reactions towards apple. AIM OF THE STUDY:We speculate that distinct IgE epitopes are responsible for the clinical manifestation of PFS. To test this hypothesis we grafted five Mal d 1 stretches onto Bet v 1. The grafted regions were 7- or 8-amino acids long encompassing amino acids residues previously shown to be crucial for IgE recognition of Bet v 1. METHODS:A Bet v 1-Mal d 1 chimeric protein designated BMC was expressed in Escherichia coli and purified to homogeneity. IgE reactivity of BMC was tested with patients' sera originating from (i) Bet v 1-allergic patients displaying no clinical symptoms upon ingestion of apples; and (ii) Bet v 1-allergic patients displaying allergic symptoms upon ingestion of apples and other Bet v 1-related foods. RESULTS AND CONCLUSION:Compared to birch pollen-allergic individuals, patients suffering from PFS showed significantly higher IgE reactivity with BMC (chimeric protein). The results suggest that the Mal d 1 regions grafted onto the Bet v 1 sequence comprise important IgE epitopes recognized by Bet v 1-allergic patients suffering from allergy to apples.






Klinglmayr E,Hauser M,Zimmermann F,Dissertori O,Lackner P,Wopfner N,Ferreira F,Wallner M




Has Abstract


2009-04-01 00:00:00
















  • Epidermal Langerhans cells in patients with primary Sjögren's syndrome.

    abstract::IgG-binding to the surface of Langerhans cells (LC) is a feature of primary Sjögren's syndrome and might be associated with qualitative and quantitative defects in LC. Fifteen patients with primary Sjögren's syndrome and 15 healthy controls had a punch biopsy performed from clinically unaffected skin. LC were enumerat...


    pub_type: 杂志文章


    authors: Oxholm P,Oxholm A,Manthorpe R

    更新日期:1986-08-01 00:00:00

  • Latex allergy in Saudi children with spina bifida.

    abstract:BACKGROUND:Children with spina bifida (SB) are exposed to latex soon after birth during bladder catheterization, rectal disimpaction, and multiple surgical procedures. IgE-mediated latex-allergic reactions have been reported recently in these children. Our study was designed to assess the prevalence of allergic reactio...


    pub_type: 杂志文章


    authors: Kattan H,Harfi HA,Tipirneni P

    更新日期:1999-01-01 00:00:00

  • Milk whey-specific immune complexes in allergic and non-allergic subjects.

    abstract::We developed an antigen- and isotype-specific ELISA for the rapid detection of native serum immune complexes (IC). It is a sandwich assay, in which a solid phase antigen-specific capture antibody selectively binds the antigen-specific IC via the antigen. The isotype of the bound IC is then identified using an enzyme-l...


    pub_type: 杂志文章


    authors: Bell SJ,Potter PC

    更新日期:1988-10-01 00:00:00

  • Long-term efficacy of preseasonal grass pollen immunotherapy in children.

    abstract:BACKGROUND:In a previous controlled study we demonstrated that preseasonal grass pollen immunotherapy for three years was effective in children. In the current study we examined the same group of patients to see if there is still a benefit six years after discontinuation of treatment. METHODS:Thirteen of 14 patients w...


    pub_type: 杂志文章


    authors: Eng PA,Reinhold M,Gnehm HP

    更新日期:2002-04-01 00:00:00

  • Dual cutaneous reactions to anti-human IgE; no change in plasma level of the split product complement C3d or effect of heparin.

    abstract::The possible dependence of the dual cutaneous reaction (DCR) on activation of the complement and coagulation systems was further elucidated. Heterologous whole anti-human IgE was injected intradermally (i.d.) on the volar aspect of the forearms of 17 drug-free healthy volunteers and venous samples for the selected lab...


    pub_type: 杂志文章


    authors: Grönneberg R

    更新日期:1985-11-01 00:00:00

  • Risk assessment for food allergy--the industry viewpoint.

    abstract::A safe food supply is a legitimate expectation of consumers, which the food industry must meet. Allergic reactions to food are recognised as a significant public health problem. Protecting allergic consumers against such reactions, while minimising the impact of food allergy on their quality of life, poses a challenge...


    pub_type: 杂志文章


    authors: Crevel RW

    更新日期:2001-01-01 00:00:00

  • Four-year stability of Der p I in house dust under simulated domestic conditions in vitro.

    abstract::For almost 4 years, the stability of Der p I was assessed by RAST inhibition in house-dust samples incubated under simulated domestic conditions (5 or 25 degrees C and 75% relative humidity). Der p I concentrations were determined before heating dust samples at 60 degrees C to kill the mites, and at 0, 6, 14, 21, and ...


    pub_type: 杂志文章


    authors: Kort HS,Kniest FM

    更新日期:1994-02-01 00:00:00

  • Staphylococcus aureus-derived extracellular vesicles induce neutrophilic pulmonary inflammation via both Th1 and Th17 cell responses.

    abstract:BACKGROUND:Recent evidence indicates that Staphylococcus aureus, one of the most important human pathogens, secretes vesicles into the extracellular milieu. OBJECTIVE:To evaluate whether inhalation of S. aureus-derived extracellular vesicles (EV) is causally related to the pathogenesis of inflammatory pulmonary diseas...


    pub_type: 杂志文章


    authors: Kim MR,Hong SW,Choi EB,Lee WH,Kim YS,Jeon SG,Jang MH,Gho YS,Kim YK

    更新日期:2012-10-01 00:00:00

  • Artificial neural network identifies nonsteroidal anti-inflammatory drugs exacerbated respiratory disease (N-ERD) cohort.

    abstract:BACKGROUND:To date, there has been no reliable in vitro test to either diagnose or differentiate nonsteroidal anti-inflammatory drug (NSAID)-exacerbated respiratory disease (N-ERD). The aim of the present study was to develop and validate an artificial neural network (ANN) for the prediction of N-ERD in patients with a...


    pub_type: 杂志文章


    authors: Tyrak KE,Pajdzik K,Konduracka E,Ćmiel A,Jakieła B,Celejewska-Wójcik N,Trąd G,Kot A,Urbańska A,Zabiegło E,Kacorzyk R,Kupryś-Lipińska I,Oleś K,Kuna P,Sanak M,Mastalerz L

    更新日期:2020-07-01 00:00:00

  • Cat, dog, and house-dust-mite allergen levels of house dust in Finnish apartments.

    abstract::The presence of indoor allergens in Finnish homes was studied for the first time. Dust samples (n = 30) were collected by vacuuming a 1 m2 area from a living-room carpet in 30 apartments divided into three groups: homes with cats (n = 10), homes with dogs (n = 10), and homes without pets (n = 10). The levels of major ...


    pub_type: 杂志文章


    authors: Raunio P,Pasanen AL,Reiman M,Virtanen T

    更新日期:1998-02-01 00:00:00

  • Sinus CT scans and mediator release in nasal secretions after nasal challenge with cypress pollens.

    abstract:BACKGROUND:Involvement of paranasal sinuses has been suggested in allergic rhinitis but not clearly demonstrated. AIMS:To investigate the relationship between intermittent allergic rhinitis and computerized tomography (CT). METHODS:Twenty patients with intermittent rhinitis and sensitized to cypress pollens underwent...


    pub_type: 杂志文章


    authors: Piette V,Bousquet C,Kvedariene V,Dhivert-Donnadieu H,Crampette L,Senac JP,Bousquet J,Demoly P

    更新日期:2004-08-01 00:00:00

  • Comparison of the effect of the beta1-blocking drugs atenolol and metoprolol on bronchial asthma.

    abstract::Twenty patients with severe or medium severe asthma were given atenolol (Atenol ICI-Pharma) and/or metoprolol (Seloken Hässle) for tachycardia, hyperkinetic tremor, arterial hypertension or symptoms of angina pectoris. These cardioselective beta-blocking drugs caused only a very slight decrease in PF values. There was...


    pub_type: 杂志文章


    authors: Carlson C,Järvinen KA

    更新日期:1978-06-01 00:00:00

  • The role of hen's egg-specific IgE, IgG and IgG4 in the diagnostic procedure of hen's egg allergy.

    abstract:BACKGROUND:Hen's egg (HE) allergy is a common disease in childhood. HE-specific serum IgE has been correlated with the outcome of oral food challenge tests, and diagnostic decision points have been described as helpful but still not sufficient to reduce the requirement for oral food challenges. The aim of the study was...


    pub_type: 杂志文章


    authors: Ahrens B,Lopes de Oliveira LC,Schulz G,Borres MP,Niggemann B,Wahn U,Beyer K

    更新日期:2010-12-01 00:00:00

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    abstract:BACKGROUND:Dissecting complex diseases in underlying distinct traits and studying these for their genetic basis might enhance the power as well as the specificity, of detection of disease genes. These phenotypes are known as intermediate phenotypes. OBJECTIVE:We were interested in the atopic basis of asthma, and used ...


    pub_type: 杂志文章


    authors: Kurz T,Altmueller J,Strauch K,Rüschendorf F,Heinzmann A,Moffatt MF,Cookson WO,Inacio F,Nürnberg P,Stassen HH,Deichmann KA

    更新日期:2005-02-01 00:00:00

  • Protein phosphatase 5 mediates corticosteroid insensitivity in airway smooth muscle in patients with severe asthma.

    abstract:BACKGROUND:The mechanisms driving glucocorticoid (GC) insensitivity in patients with severe asthma are still unknown. Recent evidence suggests the existence of GC-insensitive pathways in airway smooth muscle (ASM) caused by a defect in GC receptor (GRα) function. We examined whether other mechanisms could potentially e...


    pub_type: 杂志文章


    authors: Chachi L,Abbasian M,Gavrila A,Alzahrani A,Tliba O,Bradding P,Wardlaw AJ,Brightling C,Amrani Y

    更新日期:2017-01-01 00:00:00

  • Polymorphisms at the glutathione S-transferase, GSTP1 locus: a novel mechanism for susceptibility and development of atopic airway inflammation.

    abstract::A common feature of environmental irritants is their ability to cause local inflammation which could alter airway function. The principal targets of such injury are the epithelial cells lining the airway passages and the lower respiratory gas-exchange areas. While host atopy is a recognized risk factor for airway infl...


    pub_type: 杂志文章,评审


    authors: Spiteri MA,Bianco A,Strange RC,Fryer AA

    更新日期:2000-01-01 00:00:00

  • Anti-IgG antibodies and antinuclear antibodies in allergic patients.

    abstract::With an indirect immunofluorescence technique 77% of 96 patients with type I allergy and 40% of 20 patients with intrinsic bronchial asthma showed positive reactions for IgG anti-IgG antibodies in serum. They were present partly in an aggregated state not directly detectable before treatment with dithiothreitol. The a...


    pub_type: 杂志文章


    authors: Egeskjold EM,Permin H,Nielsen I,Sørensen HJ,Osterballe O,Kallerup HE

    更新日期:1981-11-01 00:00:00

  • Treatment of hay fever with sodium cromoglycate, hyposensitization, or a combination.

    abstract::In 77 consecutive patients with grass pollen rhinitis as the only allergic disease, treatment with sodium cromoglycate (SCG) nasal spray, hyposensitization with aluminium hydroxide adsorbed extract of grass pollen as perennial treatment, and a combination of the two was compared. Specific serum-IgE, IgG and IgG4 were ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Andersen NH,Jeppesen F,Schiøler T,Osterballe O

    更新日期:1987-07-01 00:00:00

  • ILC2 frequency and activity are inhibited by glucocorticoid treatment via STAT pathway in patients with asthma.

    abstract:BACKGROUND:Group 2 innate lymphoid cells (ILC2s) were closely associated with asthma. However, there were no perspective studies about the effects of glucocorticoid on ILC2s in asthma patients. Our objective was to perform a perspective study and evaluate the ILC2 activity after glucocorticoid therapy in asthma patient...


    pub_type: 杂志文章


    authors: Yu QN,Guo YB,Li X,Li CL,Tan WP,Fan XL,Qin ZL,Chen D,Wen WP,Zheng SG,Fu QL

    更新日期:2018-09-01 00:00:00

  • n-Nonanoyl-CCL14 (NNY-CCL14), a novel inhibitor of allergic airway inflammation is a partial agonist of human CCR2.

    abstract:BACKGROUND:Modulation of leukocyte recruitment through blocking of chemokine receptors has been proposed as an attractive therapeutic strategy. We have previously demonstrated that n-Nonanoyl-CC chemokine ligand 14 (NNY-CCL14), a modified analog of the naturally occurring chemokine CCL14(9-74) internalizes and desensit...


    pub_type: 杂志文章


    authors: Gupta S,Erdmann G,Schulz-Maronde S,Escher SE,Richter R,Forssmann WG,Elsner J,Forssmann U

    更新日期:2008-10-01 00:00:00

  • Sensitization to local dust-mite fauna in Singapore.

    abstract:BACKGROUND:Recent studies showed the presence of a unique dust-mite fauna in the indoor environment of Singapore. Immediate hypersensitivity to these dust mites, along with other known indoor allergens, may play a role in the pathogenesis of allergic respiratory diseases. This study evaluated the sensitization rates of...


    pub_type: 杂志文章


    authors: Chew FT,Lim SH,Goh DY,Lee BW

    更新日期:1999-11-01 00:00:00

  • Decreased intercellular adhesion molecule-1 (CD54) and L-selectin (CD62L) expression on peripheral blood natural killer cells in asthmatic children with acute exacerbation.

    abstract:BACKGROUND:The capacity of inflammatory cells to adhere involves an array of adhesion molecules, and is critical to the inflammatory responses seen in childhood asthma. We aimed to determine the changes of intercellular adhesion molecule-1 (ICAM-1) and L-selectin expressed on peripheral blood (PB) T lymphocytes and nat...


    pub_type: 杂志文章


    authors: Lin SJ,Chang LY,Yan DC,Huang YJ,Lin TJ,Lin TY

    更新日期:2003-01-01 00:00:00

  • Identification of allergen components of the opportunistic yeast Pityrosporum orbiculare by monoclonal antibodies.

    abstract::The yeast Pityrosporum orbiculare (P. orbiculare) is a member of the normal human cutaneous flora, but it is also associated with several clinical manifestations of the skin. We have previously observed IgE-binding components in P. orbiculare extracts, using sera from patients with atopic dermatitis. In the present st...


    pub_type: 杂志文章


    authors: Zargari A,Härfast B,Johansson S,Johansson SG,Scheynius A

    更新日期:1994-01-01 00:00:00

  • MACVIA-ARIA Sentinel NetworK for allergic rhinitis (MASK-rhinitis): the new generation guideline implementation.

    abstract::Several unmet needs have been identified in allergic rhinitis: identification of the time of onset of the pollen season, optimal control of rhinitis and comorbidities, patient stratification, multidisciplinary team for integrated care pathways, innovation in clinical trials and, above all, patient empowerment. MASK-rh...


    pub_type: 杂志文章,评审


    authors: Bousquet J,Schunemann HJ,Fonseca J,Samolinski B,Bachert C,Canonica GW,Casale T,Cruz AA,Demoly P,Hellings P,Valiulis A,Wickman M,Zuberbier T,Bosnic-Anticevitch S,Bedbrook A,Bergmann KC,Caimmi D,Dahl R,Fokkens WJ,Gris

    更新日期:2015-11-01 00:00:00

  • Is the hygiene hypothesis still a viable explanation for the increased prevalence of asthma?

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    pub_type: 杂志文章,评审


    authors: Platts-Mills TA,Erwin E,Heymann P,Woodfolk J

    更新日期:2005-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Westin U,Lundberg E,Wihl JA,Ohlsson K

    更新日期:1999-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Lebel B,Messaad D,Kvedariene V,Rongier M,Bousquet J,Demoly P

    更新日期:2001-07-01 00:00:00

  • Nasal and exhaled nitric oxide in response to occupational latex exposure.

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    pub_type: 杂志文章


    authors: Tan K,Bruce C,Birkhead A,Thomas PS

    更新日期:2001-07-01 00:00:00

  • House-dust mites and associated environmental conditions in Danish homes.

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    pub_type: 杂志文章


    authors: Harving H,Korsgaard J,Dahl R

    更新日期:1993-02-01 00:00:00

  • Arizona cypress (Cupressus arizonica) pollen allergens. Identification of cross-reactive periodate-resistant and -sensitive epitopes with monoclonal antibodies.

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    pub_type: 杂志文章


    authors: Barletta B,Tinghino R,Corinti S,Afferni C,Iacovacci P,Mari A,Pini C,Di Felice G

    更新日期:1998-06-01 00:00:00