Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa.

Abstract:

OBJECTIVE:To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. DESIGN:Prospective study. SETTING:Private infertility institute and university-based research laboratory. PATIENT(S):Eighty consenting normozoospermic patients consulting for infertility. INTERVENTION(S):Spermatozoa from donor semen samples were thawed at different temperatures. MAIN OUTCOME MEASURE(S):Sperm motility, viability, adenosine-5'-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. RESULT(S):Thawing at 40 degrees C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20 degrees C and 37 degrees C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40 degrees C compared with thawing at temperatures between 20 degrees C and 37 degrees C. CONCLUSION(S):Sperm thawing at 40 degrees C could be safely used to improve motility recovery after sperm cryopreservation.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Calamera JC,Buffone MG,Doncel GF,Brugo-Olmedo S,de Vincentiis S,Calamera MM,Storey BT,Alvarez JG

doi

10.1016/j.fertnstert.2008.10.021

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

789-94

issue

3

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(08)04283-0

journal_volume

93

pub_type

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