Abstract:
OBJECTIVE:To correlate apoptosis of cultured human granulosa-lutein cells (GL cells) with the outcome of IVF (embryo fragmentation and pregnancy rate) and to study the effect of insulin and insulin-like growth factor I (IGF-I) on apoptosis. DESIGN:In vitro assays. SETTING:University laboratory and private IVF center. PATIENT(S):Eighty-one women undergoing IVF. INTERVENTION(S):Purified human GL cells from pooled follicles were cultured for 48 hours in serum-free media with or without insulin and IGF-I. Cumulus cells and mural GL cells were evaluated separately. MAIN OUTCOME MEASURE(S):Detection of apoptosis by using caspACE FITC-VAD-FMK, a fluorescent in situ marker for activated caspases; embryo fragmentation; and pregnancy. RESULT(S):Age younger than 38 years and successful pregnancy were associated with less apoptosis (33.0% +/- 17.2% vs. 43.2% +/- 18.0% and 30.2% +/- 14.0% vs. 40.4% +/- 19.5%, respectively). There was a linear correlation between embryo fragmentation and GL cell apoptosis. Insulin-like growth factor I decreased apoptosis in a dose-dependent fashion. A statistically significant effect (17% decrease) was reached at a dose of 10 nM. Insulin (10 nM) caused a small (8%) decrease in apoptosis, but this effect did not reach statistical significance. Cumulus cells consistently had <3% apoptosis. CONCLUSION(S):[1] Apoptosis of cultured GL cells may be associated with IVF outcome and ovarian reserve and [2] IGF-I decreases apoptosis of cultured GL cells.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Bencomo E,Pérez R,Arteaga MF,Acosta E,Peña O,Lopez L,Avila J,Palumbo Adoi
10.1016/j.fertnstert.2005.08.014subject
Has Abstractpub_date
2006-02-01 00:00:00pages
474-80issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(05)03847-1journal_volume
85pub_type
杂志文章abstract::The negative correlation between the thickness of the lamina propria and Johnsen's score count in individual seminiferous tubules was observed and the tubules with thin lamina propria indicated almost complete spermatogenesis. It is suggested that the thin lamina propria may be one of the good indicators of complete s...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.10.019
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVE:To describe the option of ultrasonographic-guided percutaneous transabdominal puncture for ovum pickup in a rare case of Rokitansky syndrome with subcostal located ovaries and a right single kidney. DESIGN:Technique and instrumentation. SETTING:A university-based hospital IVF and Reproductive Medicine Unit....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.05.039
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:To assess the effects of age on the hormonal, metabolic, and ultrasonographic features of polycystic ovary syndrome (PCOS). DESIGN:Observational study. SETTING:University department of obstetrics and gynecology. PATIENT(S):Patients with PCOS (n = 1,212) and healthy women (n = 254). INTERVENTION(S):None. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.11.041
更新日期:2012-02-01 00:00:00
abstract::Menopausal hormone therapy (HT) has complex biologic effects but continues to have an important clinical role in the management of vasomotor and other menopausal symptoms. The rational use of menopausal HT requires balancing the potential benefits and risks of treatment. Findings from the Women's Health Initiative (WH...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2014.02.043
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVE:To investigate whether the embryotoxic effect of peritoneal fluid (PF) from infertile women with mild endometriosis on mouse oocytes and embryos is associated with changes in embryonic epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), and their receptors. DESIGN:Experimental animal study. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.11.011
更新日期:2010-05-15 00:00:00
abstract:OBJECTIVE:To present and determine the impact of the Mindfulness-Based Program for Infertility (MBPI). DESIGN:Controlled clinical trial. SETTING:University research unit. PATIENT(S):Fifty-five infertile women completed the MBPI, and 37 infertile women were assigned to a control group. INTERVENTION(S):The MBPI inclu...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.05.036
更新日期:2013-10-01 00:00:00
abstract:OBJECTIVE:To review a 12-year experience of the world's three largest preimplantation genetic diagnosis (PGD) centers. DESIGN:Multicenter analysis of the clinical outcome of PGD. SETTING:In vitro fertilization programs at the Reproductive Genetics Institute, Chicago, Illinois; Saint Barnabas Medical Center, West Oran...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2003.09.082
更新日期:2004-08-01 00:00:00
abstract:OBJECTIVE:To analyze the effectiveness of autologous endometrial coculture by the cycle day of the endometrial biopsy. DESIGN:Retrospective study. SETTING:University-based IVF center. PATIENT(S):Two hundred eight patients with multiple IVF failures. INTERVENTION(S):Embryos were split and randomly allocated to growt...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(02)03134-5
更新日期:2002-06-01 00:00:00
abstract:OBJECTIVE:To describe the prevalence of antiphospholipid antibodies to both anionic and zwitterionic phospholipids in women with early recurrent pregnancy losses (RPLs). DESIGN:Retrospective data analysis. SETTING:Tokai University Hospital, Kanagawa, Japan. PATIENT(S):One hundred thirty-nine patients with unexplaine...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(99)00119-3
更新日期:1999-06-01 00:00:00
abstract:OBJECTIVE:To describe a case of early pregnancy failure in a patient who was not an optimal candidate for suction aspiration because of her body habitus and history of a myocardial infarction that was treated medically with misoprostol. DESIGN:Case report. SETTING:Academic tertiary-care hospital. PATIENT:A 43-year-o...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.11.010
更新日期:2007-07-01 00:00:00
abstract:OBJECTIVE:To investigate the usefulness of dual-parameter flow cytometry of testicular fine-needle aspirates for the quantification of spermatogenesis. Immunofluorescence staining for vimentin was introduced to discriminate vimentin-negative germ cells from vimentin-positive stromal cells. The results of flow cytometry...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56656-4
更新日期:1994-04-01 00:00:00
abstract:OBJECTIVE:To investigate the possibility of genetic contribution of CYP1A1, CYP19, GSTM1, and GSTT1 polymorphisms to endometriosis. DESIGN:Genetic polymorphism analysis. SETTING:Case-control study. PATIENT(S):A group of 275 women with sporadic endometriosis was compared with a group of 346 fertile, endometriosis-fre...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04817-3
更新日期:2003-03-01 00:00:00
abstract::The distribution of genotype frequencies, allele frequencies, and haplotype frequencies of variable number of tandem repeats (VNTR) in interleukin (IL) 1RN and IL-1beta (-511C > T, exon-5C.>T) polymorphism demonstrated a positive association with the occurrence of polycystic ovary syndrome (PCOS). The carriage of alle...
journal_title:Fertility and sterility
pub_type: 信件
doi:10.1016/j.fertnstert.2009.09.042
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To correlate the diagnosis of endometriosis in lesions excised at laparoscopy with pathologic diagnosis. DESIGN:Prospective study. SETTING:U.S. government research hospital. PATIENT(S):Women with chronic pelvic pain thought to be due to endometriosis. INTERVENTION(S):Excision of lesions suspicious for end...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03337-x
更新日期:2002-10-01 00:00:00
abstract::Twenty-four women who had undergone total salpingectomy due to ectopic pregnancy and who subsequently underwent a laparotomy for a second ectopic pregnancy in their opposite tube were treated by the author. Treatment consisted of linear salpingostomy (n = 20) and partial salpingectomy (n = 4). The intrauterine pregnan...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60025-0
更新日期:1988-07-01 00:00:00
abstract:OBJECTIVE:To analyze information on assisted reproductive technology (ART) performed worldwide and trends in outcomes over successive years. DESIGN:Cross-sectional survey on access, effectiveness, and safety of ART procedures performed in 53 countries during 2005. SETTING:A total of 2,973 clinics from national and re...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.10.005
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVE:To identify if there are certain cutoff levels for P and or the P/E(2) ratio on the day of hCG that would be defined as detrimental for occurrence of pregnancy in women with normal ovarian reserve undergoing cleavage-stage embryo transfer (ET). Secondarily, to determine if these same cutoffs might have the sa...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2010.12.065
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:To demonstrate a safe and effective approach to the treatment of obstructing midline prostate utricle cyst with the use of a holmium laser. DESIGN:Video presentation. SETTING:University hospital. PATIENT(S):A 33-year-old man presented with chronic pelvic pain, pain with ejaculation, and infertility. Semen ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.08.051
更新日期:2018-12-01 00:00:00
abstract::Recent observations claimed that growth hormone (GH) administration increased the sensitivity of the ovary to gonadotropin stimulation. These findings prompted us to assess whether ovarian response to human menopausal gonadotropin (hMG) is correlated to GH reserve. Before hMG administration, 25 patients were tested fo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53336-6
更新日期:1990-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the immunoactivity of human follicular fluids (FFs) of ovaries stimulated with exogenous gonadotropins. PARTICIPANTS:Follicular fluids and peripheral blood mononuclear cells (PBMCs) were obtained at the time of ovum pick-up from 15 women (25 to 34 years of age) undergoing gonadotropin stimulation...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56886-1
更新日期:1994-08-01 00:00:00
abstract::This Committee Opinion reviews the unresolved issues and experimental nature associated with the procedure and its limited success rates. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2008.08.033
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:To describe the use of Essure microinsert placement in patients with hydrosalpinges before IVF. DESIGN:Retrospective case-series. SETTING:An advanced endoscopic practice within a university-affiliated teaching hospital. PATIENT(S):Five women with unilateral or bilateral hydrosalpinges on transvaginal ultra...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.01.050
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVE:To investigate the direct actions of active 1,25-dihydroxy vitamin D3 (VD3) upon primate follicular development at specific stages of folliculogenesis. DESIGN:Secondary preantral follicles were isolated from rhesus monkeys ovaries, encapsulated in alginate, and cultured for 40 days. Follicles were randomly a...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.08.037
更新日期:2016-12-01 00:00:00
abstract:STUDY OBJECTIVES:To assess a single (donor) sample by the use of five computer-assisted semen analysis (CASA) systems. SETTING:British Andrology Society advanced course on computer-assisted semen analysis techniques, September 1992. PARTICIPANTS:Clinical, technical and scientific personnel with mixed experience of CA...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract:OBJECTIVE:To evaluate binding characteristics of a specific zona pellucida (ZP) protein 3 (ZP3) antiserum to human oocytes in order to determine its usefulness as a clinical marker for human ZP integrity and function and its correlation with IVF outcome. DESIGN:Prospectively designed, blinded, internally controlled st...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)58041-8
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:To obtain proof-of-concept that locally produced anti-inflammatory steroids suppress ovulation-associated extracellular matrix proteases in human ovarian surface epithelial (OSE) cells. DESIGN:Primary OSE cell cultures treated with interleukin-1alpha (IL-1alpha) (500 pg/mL) as proxy for inflammation, with/wi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.06.040
更新日期:2009-08-01 00:00:00
abstract::Suppressor activity in in vitro fertilization (IVF) culture medium correlates with successful implantation. High performance liquid chromatography fractionation revealed peak(s) of inhibitory activity in the 1,000- to 5,000-Da molecular weight range. Inhibitory activity was dependent on the presence of fetal bovine se...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract:OBJECTIVE:To determine whether antisperm autoantibody production after prepubertal vas injury is influenced by immediate repair of the vas compared to delay of the reanastomosis until sexual maturity. DESIGN:Animal study comparing early repair, late repair, and sham-operated groups. SETTING:Research laboratory in a m...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(99)00501-4
更新日期:2000-02-01 00:00:00
abstract:OBJECTIVE:To evaluate if apoptosis occurs in human germ cells between 19 and 33 gestational weeks (GW). DESIGN:Human fetal ovaries were obtained from aborted fetuses aged 19-33 GW. SETTING:Rabin Medical Center, a major tertiary care and referral center. PATIENT(S):Twenty-seven women undergoing pregnancy termination....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03218-1
更新日期:2002-08-01 00:00:00
abstract:OBJECTIVE:The study was performed to evaluate the correlation between sperm motility index, a novel parameter of semen quality, and routine semen analysis parameters by microscopic evaluation. DESIGN:Sperm motility index was measured by an electro-optical device, the Sperm Quality Analyzer (United Medical Systems Inc....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54427-6
更新日期:1991-07-01 00:00:00