Abstract:
:Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.
journal_name
Fitoterapiajournal_title
Fitoterapiaauthors
Morinaga O,Uto T,Sakamoto S,Tanaka H,Shoyama Ydoi
10.1016/j.fitote.2008.09.004subject
Has Abstractpub_date
2009-01-01 00:00:00pages
28-31issue
1eissn
0367-326Xissn
1873-6971pii
S0367-326X(08)00192-5journal_volume
80pub_type
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