Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies.

Abstract:

:Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.

journal_name

Fitoterapia

journal_title

Fitoterapia

authors

Morinaga O,Uto T,Sakamoto S,Tanaka H,Shoyama Y

doi

10.1016/j.fitote.2008.09.004

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

28-31

issue

1

eissn

0367-326X

issn

1873-6971

pii

S0367-326X(08)00192-5

journal_volume

80

pub_type

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