Uptake of alpha-aminoisobutyric acid and phosphate by membrane vesicles derived from growing and quiescent fibroblasts.


:Membrane vesicles derived principally from the plasma membrane and endoplasmic reticulum of mouse 3T3 cells transformed by Simian virus 40 take up alpha-aminoisobutyric acid (AIB) and phosphate (Pi). When NaCl is added simultaneously with AIB or Pi, uptake rises two- to three-times above the equilibrium to accumulate AIB or Pi over the control value, in the presence of a Na+ gradient, is almost lost in membrane vesicles derived from benzpyrene-transformed 3T3 cells (BP3T3) arrested in the G1 phase of the cell cycle by serum starvation. When added to the membranes with NaCl and the uptake substrate, a combination of fibroblast growth factor (FGF) and epidermal growth factor EGF restores the ability of the membranes to accumulate AIB and Pi over the control value.


J Cell Physiol


Nilsen-Hamilton M,Hamilton RT




Has Abstract


1976-12-01 00:00:00












  • S100B causes apoptosis in a myoblast cell line in a RAGE-independent manner.

    abstract::S100B, a Ca(2+)-modulated protein with both intracellular and extracellular regulatory roles, is most abundant in astrocytes, is expressed in various amounts in several non-nervous cells and is also found in normal serum. Astrocytes secrete S100B, and extracellular S100B exerts trophic and toxic effects on neurons dep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sorci G,Riuzzi F,Agneletti AL,Marchetti C,Donato R

    更新日期:2004-05-01 00:00:00

  • Precursor of mast cells fixed in the skin of mice.

    abstract::The presence and origin of mast-cell precursors fixed in the skin tissue of mice were investigated. Giant granules of beige (C57BL/6-bh'/bg', Chediak-Higashi syndrome) mice were used to distinguish different populations of mast cells. Pieces of the skin were grafted from the intact WBB6F1 (WB X C57BL/6)F1-+/+ mice int...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Matsuda H,Kitamura Y,Sonoda T,Imori T

    更新日期:1981-09-01 00:00:00

  • Autocrine and paracrine growth stimulation of cells derived from human skin.

    abstract::A sensitive serum-free culture system was used to demonstrate that cells derived from normal human skin release soluble mediators which can modulate keratinocyte, melanocyte, and fibroblast growth in vitro. In M199 supplemented with epidermal growth factor, hydrocortisone, insulin, transferrin, triidothyronine, bovine...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gilchrest BA,Karassik RL,Wilkins LM,Vrabel MA,Maciag T

    更新日期:1983-11-01 00:00:00

  • Contributions of 3D Cell Cultures for Cancer Research.

    abstract::Cancer cell lines have contributed immensely in understanding the complex physiology of cancers. They are excellent material for studies as they offer homogenous samples without individual variations and can be utilised with ease and flexibility. Also, the number of assays and end-points one can study is almost limitl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Ravi M,Ramesh A,Pattabhi A

    更新日期:2017-10-01 00:00:00

  • Human bronchial epithelial cells synthesize cholesterol sulfate during squamous differentiation in vitro.

    abstract::Epithelial cells of the airways can, under pathological conditions, undergo squamous metaplasia. The accumulation of cholesterol sulfate has recently been described as a new marker for squamous cell differentiation in rabbit tracheal epithelial cells. We now report that normal human bronchial epithelial cells in cultu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rearick JI,Hesterberg TW,Jetten AM

    更新日期:1987-12-01 00:00:00

  • Upregulation of SOX11 enhances tamoxifen resistance and promotes epithelial-to-mesenchymal transition via slug in MCF-7 breast cancer cells.

    abstract::Resistance to tamoxifen remains a prominent conundrum in the therapy of hormone-sensitive breast cancer. Also, the molecular underpinnings leading to tamoxifen resistance remain unclear. In the present study, we utilized the Gene Expression Omnibus database to identify that SOX11 might exert a pivotal function in conf...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xiao Y,Xie Q,Qin Q,Liang Y,Lin H,Zeng

    更新日期:2020-10-01 00:00:00

  • MFN2 silencing promotes neural differentiation of embryonic stem cells via the Akt signaling pathway.

    abstract::Mitofusin 2 (MFN2) is a regulatory protein participating in mitochondria dynamics, cell proliferation, death, differentiation, and so on. This study aims at revealing the functional role of MFN2 in the pluripotency maintenance and primitive differetiation of embryonic stem cell (ESCs). A dox inducible silencing and ro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yi S,Cui C,Huang X,Yin X,Li Y,Wen J,Luan Q

    更新日期:2020-02-01 00:00:00

  • Resistin effects on pancreatic cancer progression and chemoresistance are mediated through its receptors CAP1 and TLR4.

    abstract::Resistin, secreted by macrophages in tumor microenvironment, has never been investigated in pancreatic cancer models, despite a vibrant tumor microenvironment around pancreatic tumors. We evaluated serum resistin levels in healthy individuals versus pancreatic cancer patients representing different tumor grades. In vi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang M,Yan L,Wang GJ,Jin R

    更新日期:2019-06-01 00:00:00

  • Leukemia stem cell immunophenotyping tool for diagnostic, prognosis, and therapeutics.

    abstract::The existence of cancer stem cells is debatable in numerous solid tumors, yet in leukemia, there is compelling evidence of this cell population. Leukemic stem cells (LSCs) are altered cells in which accumulating genetic and/or epigenetic alterations occur, resulting in the transition between the normal, preleukemic, a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Mastelaro de Rezende M,Ferreira AT,Paredes-Gamero EJ

    更新日期:2020-06-01 00:00:00

  • The glioma cell-derived neurite promoting activity protein is functionally and immunologically related to human protease nexin-I.

    abstract::Protease nexin-I (PN-I, Mr approximately 43,000) is representative of a newly described class of cell-secreted protease inhibitors. PN-I has been purified to apparent homogeneity, partially sequenced, and monospecific antibodies have been raised against it. PN-I is a potent inhibitor of urokinase, thrombin, plasmin, a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Knauer DJ,Orlando RA,Rosenblatt D

    更新日期:1987-08-01 00:00:00

  • Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells.

    abstract::The expression profile and functions of the brain-enriched Rab22B/Rab31 small GTPase had remained uncharacterized. Using specific antibodies against Rab22B, we found the protein to be exceptionally enriched in nestin and RC2-positive radial glia of the embryonic mouse brain. In the adult brain, Rab22B is rather specif...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ng EL,Ng JJ,Liang F,Tang BL

    更新日期:2009-12-01 00:00:00

  • Dominant and recessive mitogen-nonproliferative variants of 3T3 cells.

    abstract::We have previously isolated 3T3 cell variants unable to respond to specific mitogens. In this report we analyze the dominant and/or recessive nature of these variants. Two independently isolated EGF nonproliferative variants are unable to bind EGF. Hybrids between 3T3R5 cells (thymidine kinase deficient, ouabain-resis...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Terwilliger E,Herschman HR

    更新日期:1985-06-01 00:00:00

  • Functional role of Runx3 in the regulation of aggrecan expression during cartilage development.

    abstract::Runx2 and Runx3 are known to be expressed in the growth plate during endochondral bone formation. Here we addressed the functional role of Runx3 as distinct from Runx2 by using two models of postnatal bone repair: fracture healing that proceeds by an endochondral process and marrow ablation that proceeds by only an in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wigner NA,Soung do Y,Einhorn TA,Drissi H,Gerstenfeld LC

    更新日期:2013-11-01 00:00:00

  • Core Binding Factor β Plays a Critical Role During Chondrocyte Differentiation.

    abstract::Core binding factor β (Cbfβ) is a partner protein of Runx family transcription factors with minimally characterized function in cartilage. Here we address the role of Cbfβ in cartilage by generating chondrocyte-specific Cbfβ-deficient mice (Cbfb(Δch/Δch) ) from Cbfb-floxed mice crossed with mice expressing Cre from th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Park NR,Lim KE,Han MS,Che X,Park CY,Kim JE,Taniuchi I,Bae SC,Choi JY

    更新日期:2016-01-01 00:00:00

  • Relationship between c-Kit expression and proliferation in acute myeloblastic leukemia cell lines.

    abstract::We have proposed that the transmembrane receptor encoded by the c-Kit protooncogene and its ligand play an important role in regulating the proliferation of blasts cells in acute myeloblastic leukemia (AML). To test this hypothesis, immunobeads were used to separate blasts from three Kit-expression positive cell lines...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tohda S,Yang GS,Ashman LK,McCulloch EA,Minden MD

    更新日期:1993-02-01 00:00:00

  • Glycochenodeoxycholate induces cell survival and chemoresistance via phosphorylation of STAT3 at Ser727 site in HCC.

    abstract::Glycochenodeoxycholate (GCDA) is closely associated with carcinogenesis and chemoresistance of hepatocellular carcinoma (HCC). Signal transducer and activator of transcription 3 (STAT3), a transcription factor, is involved in various human tumors. Whether GCDA induces chemoresistance through STAT3 and the mechanism of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang J,Zhou M,Jin X,Li B,Wang C,Zhang Q,Liao M,Hu X,Yang M

    更新日期:2020-03-01 00:00:00

  • Renal effects of general anesthesia from old to recent studies.

    abstract::Various types of anesthesia are being utilized to maintain physiologically secured surgical conditions. Nearly all categories of general anesthesia are characterized by various perioperative and postoperative complications. These shortcomings are important aspects that need to be considered by the anesthesiologist and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Alizadeh R,Fard ZA

    更新日期:2019-08-01 00:00:00

  • Ferric-salicylaldehyde isonicotinoyl hydrazone, a synthetic iron chelate, alleviates defective iron utilization by reticulocytes of the Belgrade rat.

    abstract::The Belgrade rat has a hypochromic, microcytic anemia inherited as an autosomal recessive mutation. Although transferrin binds normally to reticulocytes and internalizes normally, iron accumulation into cells and heme is much slower than normal. We have investigated the role of the transferrin cycle in this mutant by ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Garrick LM,Gniecko K,Hoke JE,al-Nakeeb A,Ponka P,Garrick MD

    更新日期:1991-03-01 00:00:00

  • The role of autophagy in bone homeostasis.

    abstract::Autophagy is an evolutionarily conserved intracellular process and is considered one of the main catabolism pathways. In the process of autophagy, cells are digested nonselectively or selectively to recover nutrients and energy, so it is regarded as an antiaging process. In addition to the essential role of autophagy ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Guo YF,Su T,Yang M,Li CJ,Guo Q,Xiao Y,Huang Y,Liu Y,Luo XH

    更新日期:2021-01-16 00:00:00

  • Biosensors for real-time monitoring of physiological processes in the musculoskeletal system: A systematic review.

    abstract::Biosensors are composed of (bio)receptors, transducers, and detection systems and are able to convert the biological stimulus into a measurable signal. This systematic review evaluates the current state of the art of innovation and research in this field, identifying the biosensors that in vitro monitor the musculoske...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Veronesi F,Tschon M,Visani A,Fini M

    更新日期:2019-12-01 00:00:00

  • Galactosemia: the good, the bad, and the unknown.

    abstract::Alpha-D-galactose is metabolized in species ranging from E. coli to mammals predominantly via a series of sequential reactions collectively known as the Leloir pathway. Deficiency of any one of these enzymes in humans results in a form of the inherited metabolic disorder, galactosemia, although the symptoms and severi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Fridovich-Keil JL

    更新日期:2006-12-01 00:00:00

  • Factors influencing calcium-induced terminal differentiation in cultured mouse epidermal cells.

    abstract::Mouse epidermal cells can be grown as a proliferating monolayer in medium containing 0.02-0.1 mM calcium. Terminal differentiation of these cells with formation of cornified cells and cell death is induced by elevating calcium in the medium to greater than 0.1 mM. A variety of agents were studied as potential modifier...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hennings H,Holbrook KA,Yuspa SH

    更新日期:1983-09-01 00:00:00

  • Low oxygen tension increases mRNA levels of alpha 1 (I) procollagen in human dermal fibroblasts.

    abstract::Dermal fibroblasts exposed to low oxygen tension show upregulated synthesis of transforming growth factor-beta 1 (TGF-beta 1), an established stimulatory peptide in the formation of extracellular matrix proteins. In this report, procollagen synthesis was measured in cultures of confluent adult human dermal fibroblasts...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Falanga V,Martin TA,Takagi H,Kirsner RS,Helfman T,Pardes J,Ochoa MS

    更新日期:1993-11-01 00:00:00

  • Heparan sulfates of mouse cells. Analysis of parent and transformed 3T3 cell lines.

    abstract::Heparan sulfate from the surface of a variety of mouse cells at different cell densities was examined by ion-exchange chromatography. The results of this analysis show that: (1) The heparan sulfate from new isolates of Swiss 3T3 cells transformed by SV40 virus (a DNA tumor virus) elutes from DEAE-cellulose at a lower ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Underhill CB,Keller JM

    更新日期:1977-01-01 00:00:00

  • Rapid in vitro formation of smooth endoplasmic reticulum aggregates within peptide-producing islet cells.

    abstract::We report here that heptanol (3.5 mM) induces in vitro a rapid formation of smooth endoplasmic reticulum aggregates (SERA) within isolated islets of Langerhans. SERA appeared after only 15 min of exposure to the alkanol and increased in number during the first 30 min of incubation. At that time, SERA represented 2% an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Knodel S,Meda P,Orci L

    更新日期:1987-10-01 00:00:00

  • Calcium uptake by isolated rat intestinal cells.

    abstract::Intestinal cells were isolated by a combination of mechanical and enzymatic means, and their calcium uptake was assayed by a rapid filtration procedure. Calcium uptake was a time- and concentration-dependent process that was markedly elevated at 25 and 37 degrees C, as compared to 0 degree C. Cells isolated from rat d...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bronner F,Pansu D,Bosshard A,Lipton JH

    更新日期:1983-09-01 00:00:00

  • Intracellular localization of ClC chloride channels and their ability to form hetero-oligomers.

    abstract::ClC chloride channels (ClCs) can be classified into two groups in terms of their cellular localizations: ClCs present in the plasma membranes and those residing in intracellular organelles. Members of the latter group, including ClC-3, ClC-4, ClC-5, ClC-6, and ClC-7, are often co-expressed in a variety of cell types i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Suzuki T,Rai T,Hayama A,Sohara E,Suda S,Itoh T,Sasaki S,Uchida S

    更新日期:2006-03-01 00:00:00

  • Sulfated malto-oligosaccharides bind to basic FGF, inhibit endothelial cell proliferation, and disrupt endothelial cell tube formation.

    abstract::The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Oligosaccharides based on heparin structures, as small as six sugar residues, have been demonstrated to bind to bFGF and block its activity, whi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Foxall C,Wei Z,Schaefer ME,Casabonne M,Fugedi P,Peto C,Castellot JJ Jr,Brandley BK

    更新日期:1996-09-01 00:00:00

  • TGF beta alters growth and differentiation related gene expression in proliferating osteoblasts in vitro, preventing development of the mature bone phenotype.

    abstract::This study examines the mechanism by which TGF-beta 1, an important mediator of cell growth and differentiation, blocks the differentiation of normal rat diploid fetal osteoblasts in vitro. We have established that the inability for pre-osteoblasts to differentiate is associated with changes in the expression of cell ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Breen EC,Ignotz RA,McCabe L,Stein JL,Stein GS,Lian JB

    更新日期:1994-08-01 00:00:00

  • 1,7-Bis(4-hydroxyphenyl)-1,4-heptadien-3-one induces lung cancer cell apoptosis via the PI3K/Akt and ERK1/2 pathways.

    abstract::1,7-Bis(4-hydroxyphenyl)-1,4-heptadien-3-one (EB30) is a diarylheptanoid-like compound isolated from Viscum coloratum. This curcumin analog exhibits significant cytotoxic activity against HeLa, SGC-7901, and MCF-7 cells. However, little is known about the anticancer effects and mechanisms of EB30 in human lung cancer....

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fan J,Wu M,Wang J,Ren D,Zhao J,Yang G

    更新日期:2019-05-01 00:00:00