Screening for bacterial pathogens in semen samples from infertile men with and without leukocytospermia.

Abstract:

:We aimed to study the correlation between leukocyte counts in semen and bacterial pathogens in seminal samples of infertile men, and to establish the minimum leukocyte count associated with significant bacteriospermia. A total of 116 patients who underwent evaluation of fertility were investigated using routine semen analysis according to the guidelines of the WHO and bacterial pathogens analysis by culture and in-house PCR assay. The overall prevalence of bacteriospermia in semen samples was 56.9% independent of the presence of leukocytes. The most common bacterial species detected were Chlamydia trachomatis (41.4%), Ureaplasma urealyticum (15.5%) and Mycoplasma hominis (10.3%). The receiver operating characteristic curve analysis demonstrated that the sensitivity/specificity for detecting bacteria at a cut off level of >or=1 x 10(6) leukocytes per ml (which is the WHO defined level for leukocytospermia) was 20.3%/81.5%. The highest sensitivity/specificity ratio was found in semen samples with a cut-off level of >or=0.275 x 10(6) leukocytes per ml, which is best shown with the odds ratio of 2.47. A significant correlation was found between bacteriospermia and leukocytospermia at the cut-off level of >or=0.275 x 10(6) leukocytes per ml of semen samples (P = 0.032). We proposed that this is a possible new cut-off level to predict the presence of bacteria in semen of infertile men.

journal_name

Andrologia

journal_title

Andrologia

authors

Gdoura R,Kchaou W,Znazen A,Chakroun N,Fourati M,Ammar-Keskes L,Hammami A

doi

10.1111/j.1439-0272.2008.00845.x

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

209-18

issue

4

eissn

0303-4569

issn

1439-0272

pii

AND845

journal_volume

40

pub_type

杂志文章
  • Adequacy of hyaluronan binding assay and a new fertility index derived from it for measuring of male fertility potential and the efficacy of supplement therapy.

    abstract::The aim of the study was to statistically prove that the HBA(®) test is an efficient practical method for andrologists to determine the fertility potential as well as to measure the efficiency of oral supplement therapy in case of male infertility. In the study, 175 patients were involved and it also included the foll...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12296

    authors: Szucs M,Osvath P,Laczko I,Jakab A

    更新日期:2015-06-01 00:00:00

  • Anti-androgenic, anti-oxidant and anti-apoptotic effects of the aqueous and methanol extracts of Pterorhachis zenkeri (Meliaceae): Evidence from in vivo and in vitro studies.

    abstract::The aim of this study was to evaluate the effects of Pterorhachis zenkeri (Meliaceae) on sex organ growth in immature male rats and, oxidative stress and apoptosis markers in CCL-97 (R2C) Leydig cells. For the in vivo studies, 70 immature male Wistar rats (n = 10/group) were treated for 2 or 4 weeks with: distilled wa...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13815

    authors: Bonsou Fozin GR,Deeh Defo PB,Wankeu-Nya M,Ngadjui E,Kamanyi A,Watcho P

    更新日期:2020-12-01 00:00:00

  • Relationships between personality traits, seminal parameters and hormones in male infertility.

    abstract::In this study we investigated the relationship between personality attitudes, psychopathological symptoms and biological parameters in male infertility. Eighty-four infertile men underwent a psychological and medical examination at our clinic. The psychological tests comprised the Symptom Checklist 90-R, the Toronto A...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:

    authors: Conrad R,Schilling G,Haidl G,Geiser F,Imbierowicz K,Liedtke R

    更新日期:2002-10-01 00:00:00

  • Molecular characterisation of a case of dicentric Y presented as nonobstructive azoospermia with testicular early maturation arrest.

    abstract::The dicentric Y chromosome is the most common cytogenetically visible structural abnormality of Y chromosome. The sites of break and fusion of dicentric Y are variable, but break and fusion at Yq12 (proximal to the pseudoautosomal region 2/PAR 2) is very rare. Dicentric Y chromosome is unstable during cell division an...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12886

    authors: Kumar P,Jain M,Kalsi AK,Halder A

    更新日期:2018-03-01 00:00:00

  • Antioxidant enzyme activity and MDA level in the rat testis following chronic administration of ghrelin.

    abstract::Ghrelin has recently been reported to exert beneficial effects on various oxidative stresses as a result of its antioxidant properties. Therefore, we designed this study to explore the probable antioxidative effects of this peptide in the testis. Twenty-eight male adult Wistar rats were divided into equal control and ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2009.00932.x

    authors: Kheradmand A,Alirezaei M,Asadian P,Rafiei Alavi E,Joorabi S

    更新日期:2009-12-01 00:00:00

  • Chromosome variability and germ cell development in the house mouse.

    abstract::Structural heterozygosities of the karyotype have detrimental effects on the meiotic process, resulting very often in impairment of fertility in the carriers. Both male and female germ cell development are affected by chromosomal variability although spermatogenesis seems particularly prone to be affected, probably be...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1992.tb02601.x

    authors: Redi CA,Garagna S

    更新日期:1992-01-01 00:00:00

  • Sperm DNA methylation of H19 imprinted gene and male infertility.

    abstract::Infertility affects up to 15% of reproductive-aged couples worldwide, with male factor being detected in 40%-50% of the cases. Proper sperm production is associated with the establishment of appropriate epigenetic marks in developing germ cells. Several studies have demonstrated the association between abnormal sperma...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12766

    authors: Nasri F,Gharesi-Fard B,Namavar Jahromi B,Farazi-Fard MA,Banaei M,Davari M,Ebrahimi S,Anvar Z

    更新日期:2017-12-01 00:00:00

  • Black soot exposure induced hypothalamic and testicular oxidative stress and apoptosis in male rats.

    abstract::Air pollution constitutes the largest cause of environmental risks today. At present, no scientific publication linking environmental black soot and derangement in the hypothalamus and testis of rats exists. This study investigated the effect of black soot exposure on hypothalamic and testicular functions of male rats...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13866

    authors: Onyeso G,Bohr EL,Nkpaa KW,Amadi PN,Ugwu AC

    更新日期:2020-12-01 00:00:00

  • Microcinematographic analysis of the motility of human spermatozoa incubated with caffeine.

    abstract::The effects of incubation with caffeine at final concentration of 0.5 and 5.0 mM for 30 min at ambient temperature were examined in three normal semen samples and three from asthenozoospermic men. A 50 frames/s microcinematographic analysis of the sperm motility was performed and two characteristic parameters of the t...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1982.tb02295.x

    authors: Serres C,Feneux D,David G

    更新日期:1982-09-01 00:00:00

  • Non-Robertsonian translocation t (2;11) is associated with infertility in an oligospermic man.

    abstract::Infertility is a major health problem which affects approximately 22% of married couples in reproductive age. Chromosomal defects are the most common genetic abnormalities in infertile men, with an incidence of cytogenetic abnormalities ranging from 2.1% to 15.5%. We describe here the clinical and cytogenetic studies ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12120

    authors: Ananthapur V,Avvari S,Veena K,Sujatha M,Jyothy A

    更新日期:2014-05-01 00:00:00

  • Disordered APC/C-mediated cell cycle progression and IGF1/PI3K/AKT signalling are the potential basis of Sertoli cell-only syndrome.

    abstract::The cause of Sertoli cell-only syndrome (SCOS), a condition in which only Sertoli cells line the seminiferous tubules in the testis, is unknown. Three microarray data sets were downloaded from public databases and were used to compare SCOS and control group. A total of 291 genes differentially expressed (Log2 |FC| ≥ 1...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13288

    authors: Zhang H,Zhou D,Zhu F,Chen F,Zhu Y,Yu R,Fan L

    更新日期:2019-08-01 00:00:00

  • Sperm motile efficiency (SME) of fresh spermatozoa in a hypotonic medium correlates with their cryosurvival in fertile men and infertility patients (predicting test).

    abstract::The sperm motile efficiency (SME) of fresh spermatozoa in a hypotonic medium was investigated concerning the prediction of post thaw motility and the differentiation between fertile men and infertility patients. A total of 95 semen samples were collected for this study. Twenty of these samples were obtained from semen...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1989.tb02425.x

    authors: Glander HJ,Bock H,Winiecki P

    更新日期:1989-07-01 00:00:00

  • Enzymatic properties of adult human Sertoli cells in vitro.

    abstract::The adult human Sertoli cells produced lactate, estradiol-17beta, transferrin and inhibin; germ cells modulate synthesis of these compounds. In order to study the functional features of human Sertoli cells in vitro, the aim of this study was to measure the lactic dehydrogenase (LDH), gamma-glutamyl transpeptidase (GGT...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1996.tb02762.x

    authors: Carreau S,Drosdowsky MA,Foucault P

    更新日期:1996-03-01 00:00:00

  • Grape seed extract attenuates dexamethasone-induced testicular and thyroid dysfunction in male albino rats.

    abstract::This study scrutinised the ameliorative properties of grape seed extract (GSE) in dexamethasone (DEX)-induced testicular and thyroid dysfunction associated with oxidative stress in male albino rats. Thirty-two healthy adult male albino rats were divided into four groups of eight animals each: normal, DEX control, DEX ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13002

    authors: Hasona NA

    更新日期:2018-06-01 00:00:00

  • Seminal parameters of chronic male genital inflammation are associated with disturbed sperm DNA integrity.

    abstract::Definition of chronic male genital tract inflammation and its impact on male infertility is still a matter of debate. In particular, DNA integrity has been reported to be disturbed in subfertile men. Thus, the aim of this study was to investigate an association of DNA integrity to altered standard semen parameters as ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12408

    authors: Haidl F,Haidl G,Oltermann I,Allam JP

    更新日期:2015-05-01 00:00:00

  • Comparative cytomorphologic aspects of the male germ cells, especially of the "Gonia".

    abstract::During early development of the human testis the male germ cell first appears as primordial germ cell or gonocyte and enters the prospermatogonial stage after the twelfth week of gestation. Prospermatogonia lack glycogen particles and are connected by intercellular bridges. During the same period in the ovary the fema...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1976.tb02120.x

    authors: Wartenberg H

    更新日期:1976-01-01 00:00:00

  • The synthetic androgen mibolerone induces transient suppression of the transformed phenotype in an androgen responsive human prostatic carcinoma cell line.

    abstract::The synthetic androgen mibolerone elicits a set of distinct changes in the behaviour of an androgen responsive human prostatic carcinoma cell line (LNCaP). Inhibition of cell proliferation, induction of morphological change and of a prostate specific mRNA, and inhibition of colony formation in soft agar are induced by...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1990.tb02071.x

    authors: Schulz P,Wolf D,Arbusow V,Bojar H,Klobeck HG,Fittler F

    更新日期:1990-01-01 00:00:00

  • Prevalence of low serum cobalamin in infertile couples.

    abstract::A high prevalence of low levels of cobalamin had been found in a survey of multi-ethnic normal individuals in Israel. The purpose of this study was to investigate the incidence of cobalamin deficiency among Israeli couples suffering from infertility. All couples seen at the in vitro fertilization clinic at an urban ho...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2008.00895.x

    authors: Pront R,Margalioth EJ,Green R,Eldar-Geva T,Maimoni Z,Zimran A,Elstein D

    更新日期:2009-02-01 00:00:00

  • Significance of CFTR gene mutations in patients with congenital aplasia of vas deferens with special regard to renal aplasia.

    abstract::Between 1994 and 2010, a total of 123 patients with obstructive azoospermia due to aplasia of vas deferens (CAVD) were surgically treated. In 110 patients, the condition was bilateral (CBAVD), 13 men had unilateral aplasia (CUAVD), and 10 patients additionally had aplasia of one kidney. All patients underwent CFTR gen...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2012.01281.x

    authors: Schwarzer JU,Schwarz M

    更新日期:2012-10-01 00:00:00

  • Effect of flutamide and two novel synthetic steroids on GABA, glutamine and some oxidative stress markers in rat brain and prostate.

    abstract::Flutamide is a steroid used to treat androgen-dependent disorders and as antiepileptic, but it induces a number of non-desirable side effects. This work was aimed at assaying the effect of flutamide and two novel synthetic steroids on the levels of GABA, glutamine and oxidative stress markers. Male Wistar rats (weight...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2010.01051.x

    authors: Calderón Guzmán D,Bratoeff E,Ramírez López E,Hernández García E,Pierdant Rioja F,Osnaya Brizuela N,Trujillo Jiménez F,Barragán Mejía G,Juárez Olguín H,Santamaría Del Ángel D

    更新日期:2011-08-01 00:00:00

  • The ameliorative effects of methylene blue on testicular damage induced by cisplatin in rats.

    abstract::Cisplatin, a common chemotherapeutic drug, can induce testicular toxicity. Methylene blue, a potent antioxidant, can inhibit the generation of free radicals. This research aimed to study the protective effect of methylene blue against the cisplatin-induced toxicity of the reproductive system in rats. 35 male Wistar ra...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13850

    authors: Gholami Jourabi F,Yari S,Amiri P,Heidarianpour A,Hashemi H

    更新日期:2021-02-01 00:00:00

  • The medical treatment of male infertility.

    abstract::Mesterolone, HCG and PMS, clomiphene and various other drugs were administered to 236 subfertile patients with disturbed spermatogenesis. Most of the patients received mesterolone. The effects of the various therapeutic regimes are discussed. Improvement of the sperm count and spermatozoal motility was more frequent a...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1983.tb00205.x

    authors: Ibrahim A,Hayat ZG

    更新日期:1983-01-01 00:00:00

  • Determination of sperm-antibodies in sera of sterile women by affinity-chromatography (affinity-chromatography of sperm-antibodies).

    abstract::The total amount of humoral sperm antibody protein is determined following its isolation by affinity-chromatography on spermatozoa. Serum proteins are eluted by Sorensen phosphate buffer. Antibodies attached to spermatozoa suspended in Sephadex G 75 columns resist elution by Sorensen buffer. They can be quantitatively...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1975.tb01236.x

    authors: Gradl T,Mettler L,Schirren C

    更新日期:1975-01-01 00:00:00

  • Effect of nonylphenol on spermatogenesis: A systematic review.

    abstract::Nonylphenol (NP) is known as an environmental pollutant that has adverse effects on the spermatogenesis process. In this review, we focus on (1999-2020) studies on the effect of this pollutant on the sperm parameters and the male reproductive system. Spermatogenesis is a process in which male spermatogonia (primary ge...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13748

    authors: Malmir M,Faraji T,Ghafarizadeh AA,Khodabandelo H

    更新日期:2020-07-14 00:00:00

  • Leptin exists in tubuli seminiferi and in seminal plasma.

    abstract::Leptin is a 167-amino acid protein that stimulates gonadotrophin-releasing hormone secretion and exerts indirect effects on the gonads via neuropeptide Y, NPY. Recent research has suggested that leptin may also have an effect on testosterone secretion. To investigate the role of leptin in reproduction, leptin in testi...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1046/j.1439-0272.2002.00501.x

    authors: Glander HJ,Lammert A,Paasch U,Glasow A,Kratzsch J

    更新日期:2002-09-01 00:00:00

  • Leydig cell function and morphology in the rat testis after exposure to heat.

    abstract::Leydig cell function, as judged from testosterone concentrations in plasma and in testicular tissue after LH stimulation, was tested in adult rat testes 4 days after brief exposure of the scrotum to 33 degrees and 43 degrees C, respectively. There was no significant difference in basal plasma testosterone concentratio...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1980.tb00568.x

    authors: Damber JE,Bergh A,Janson PO

    更新日期:1980-01-01 00:00:00

  • The effect of kallikrein on spermatozoal ATP and other semen parameters.

    abstract::Kallikrein promotes the release of kinins, which have been shown in in vitro studies to improve motility, viability and metabolism in human spermatozoa. The effects of kallikrein therapy on 25 males attending an infertility clinic was assessed, regarding endogenous spermatozoal ATP content, as well as other semen para...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1987.tb02345.x

    authors: Berzin M,Bilgeri YR,Zanzinger A,Wing J,Kalk WJ,Mendelsohn D

    更新日期:1987-06-01 00:00:00

  • Reproductive immunology: 4. The effect of seminal plasma from immunologic infertile men on in vitro lymphocyte cultures.

    abstract::The seminal plasma of 31 patients were examined for the presence of sperm antibodies and for inhibitory properties on the transformation rates of donor lymphocytes cultured in vitro. Twenty five (80%) of the men revealed apart from fertile semen, no sperm antibody activities while six (20%) patients showed high sperma...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1982.tb03105.x

    authors: Franken DR,Slabber CF

    更新日期:1982-03-01 00:00:00

  • Experience with the Jonas penile prosthesis in the treatment of impotence.

    abstract::The penile prosthesis has become established as a mode of therapy for organic and chronic psychogenic impotence. The Jonas type of penile prosthesis has been implanted into 10 patients and has achieved both functional success and excellent acceptance by the partner. Mechanical and pathological complications have a ver...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1984.tb00277.x

    authors: Reisner GS,Lording DW,Rose N

    更新日期:1984-05-01 00:00:00

  • The relationship between ejaculated ram sperm mitochondrial protein synthesis and motility.

    abstract::D-chloramphenicol, at concentrations of 20 and 40 micrograms/ml, inhibited over 80% of the newly synthesized mitochondrial proteins expressed by incorporation of 3H-amino acid mixture. At concentrations of 20 and 40 micrograms ml-1, D-chloramphenicol enhanced the collective motility of washed ram spermatozoa. The coll...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1994.tb00815.x

    authors: Twaina-Bechor E,Bartoov B

    更新日期:1994-11-01 00:00:00