Determination of sperm-antibodies in sera of sterile women by affinity-chromatography (affinity-chromatography of sperm-antibodies).

Abstract:

:The total amount of humoral sperm antibody protein is determined following its isolation by affinity-chromatography on spermatozoa. Serum proteins are eluted by Sorensen phosphate buffer. Antibodies attached to spermatozoa suspended in Sephadex G 75 columns resist elution by Sorensen buffer. They can be quantitatively washed out by 8 ml Tris HCl-buffer pH 8,9 and be determined as proteins by the Folin-Ciocalteu method. :A procedure for the determination of sperm-antibodies in sera of sterile women by affinity-chromatography is presented. In this procedure intact spermatozoa serve as antigen carriers, serum proteins are eluted by Sorensen phosphate buffer, and antibodies attached to spermatozoa suspended in Sephadex G 75 columns resist elution by Sorensen buffer. The antibodies can be quantitatively washed out with 8 ml of Tris hydrochloric acid buffer pH 8,9 and be determined by the Folin-Ciocalteu method. The adsorption isotherm results from plotting the applied serum dilutions against eluted antibody reveal a linear course within a range of 200-500 mcg protein/ml serum sample.

journal_name

Andrologia

journal_title

Andrologia

authors

Gradl T,Mettler L,Schirren C

doi

10.1111/j.1439-0272.1975.tb01236.x

keywords:

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

99-104

issue

2

eissn

0303-4569

issn

1439-0272

journal_volume

7

pub_type

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