Abstract:
:The total amount of humoral sperm antibody protein is determined following its isolation by affinity-chromatography on spermatozoa. Serum proteins are eluted by Sorensen phosphate buffer. Antibodies attached to spermatozoa suspended in Sephadex G 75 columns resist elution by Sorensen buffer. They can be quantitatively washed out by 8 ml Tris HCl-buffer pH 8,9 and be determined as proteins by the Folin-Ciocalteu method. :A procedure for the determination of sperm-antibodies in sera of sterile women by affinity-chromatography is presented. In this procedure intact spermatozoa serve as antigen carriers, serum proteins are eluted by Sorensen phosphate buffer, and antibodies attached to spermatozoa suspended in Sephadex G 75 columns resist elution by Sorensen buffer. The antibodies can be quantitatively washed out with 8 ml of Tris hydrochloric acid buffer pH 8,9 and be determined by the Folin-Ciocalteu method. The adsorption isotherm results from plotting the applied serum dilutions against eluted antibody reveal a linear course within a range of 200-500 mcg protein/ml serum sample.
journal_name
Andrologiajournal_title
Andrologiaauthors
Gradl T,Mettler L,Schirren Cdoi
10.1111/j.1439-0272.1975.tb01236.xkeywords:
subject
Has Abstractpub_date
1975-01-01 00:00:00pages
99-104issue
2eissn
0303-4569issn
1439-0272journal_volume
7pub_type
杂志文章相关文献
ANDROLOGIA文献大全abstract::This study investigated the effects of rutin against reproductive damage caused by toxic mercury in male rats. Thirty-five Sprague Dawley rats were used. Control group was injected with saline for 7 days. The rutin-100 group received 100 mg/kg/b.w. rutin for 7 days. Mercuric chloride (HgCl2 ) group received 1.23 mg/kg...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13524
更新日期:2020-04-01 00:00:00
abstract::Leydig cells and seminiferous tubules of human testicular tissue were successfully separated by means of an anti-substance P-antiserum. On incubation with (14C)-testosterone or (14C)-dihydrotestosterone (DHT) it could be shown that besides DHT and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) also 5 alpha-an...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Although histamine has been suggested to be involved in the control of male sexual function, including the induction of penile erection, its role in the human corpus cavernosum penis is still poorly understood. The aim of our study was to evaluate the course of histamine plasma levels through different stages of sexua...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2011.01222.x
更新日期:2012-05-01 00:00:00
abstract::Little is known about the biological and clinical significance of bilaterally enlarged testes (bilateral megalotestes or macroorchidism). We have investigated clinical, endocrine and spermatological characteristics of 135 andrological patients with a testicular volume of more than 25 ml bilaterally. 135 patients with ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1995.tb01094.x
更新日期:1995-07-01 00:00:00
abstract::The association of dietary nutrient patterns and sperm motility is not yet well elucidated, and previous studies have just focused on the isolated nutrients. This case-control study examined the association of nutrient patterns with asthenozoospermia among Iranian men. In total, 107 incident asthenozoospermic men and ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12624
更新日期:2017-04-01 00:00:00
abstract::One prerequisite for fertilization is the specific binding of spermatozoa to the zona pellucida. However, the factors and mechanisms involved in this gamete contact are not well understood. Gamete recognition and binding are species-specific and are controlled by oligosaccharides of the zona and their corresponding ca...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.2001.00412.x
更新日期:2001-09-01 00:00:00
abstract::Multiple number of techniques and devices have been described concerning circumcision method so far. One of them is thermocautery device, and it has been widely used. Although there is controversy that the penis may be damaged due to the heat generated during the use of the device, not enough histopathological studies...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13968
更新日期:2021-01-09 00:00:00
abstract::Our previous study found that Chinese chive could significantly (p < 0.01) raise testosterone and nitric oxide (NO) levels in mice serum. However, the specific functional components of this traditional remedy are still unknown. In order to isolate and identify the active constituents from Chinese chive for enhancing t...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13191
更新日期:2019-03-01 00:00:00
abstract::We aimed to study the correlation between leukocyte counts in semen and bacterial pathogens in seminal samples of infertile men, and to establish the minimum leukocyte count associated with significant bacteriospermia. A total of 116 patients who underwent evaluation of fertility were investigated using routine semen ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2008.00845.x
更新日期:2008-08-01 00:00:00
abstract::Methamphetamine has been reported to be an important drug in the field of reproductive toxicology. The aim of this study was to investigate the effects of methamphetamine administrations on sperm morphology, sperm concentration and apoptotic activity inside seminiferous tubule in male rats. Rats were administered a do...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2010.01071.x
更新日期:2011-08-01 00:00:00
abstract::The possible physiological role of testicular corticotropin-releasing factor (CRF) in the regulation of testicular functions was studied in neonatal rats. Two microlitres of anti-CRF-antiserum (dilution: 1:10 or 1:100) was injected intratesticularly to 5 d-old rats with two testes and to hemicastrates. Five days after...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1993.tb02711.x
更新日期:1993-07-01 00:00:00
abstract::By means of a recently developed apparatus ("Separon") motile spermatozoa were concentrated in a small volume of culture medium (0,3 ml). Sperm concentration, progressive motility and mean velocity of 60 ejaculates were determined by a computerized videomicrography system. After "Separon" sperm concentration was reduc...
journal_title:Andrologia
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::A number of studies indicated a clear decline in semen quality in the past 30-50 years and there is accumulating evidence that this decline might result from exposure to high levels of air pollution. To examine the impact of environment on male reproductive ability, we undertook for the first time a pilot study on sem...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.2008.00909.x
更新日期:2009-06-01 00:00:00
abstract::Multiple morphological abnormalities of the sperm flagella (MMAF) is a rare disease associated with male infertility. In our previous study, we identified a homozygous CFAP43 splice-site variant, c.3661-2delA, in a patient with MMAF. However, the mutational effect of this variant was unknown. Here, using a minigene as...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13575
更新日期:2020-07-01 00:00:00
abstract::Cryptorchidism is the most common congenital anomaly in male children. Its aetiology remains unknown in the majority of cases. Because HOXA11 plays a vital role in regulating testicular descent, genetic variants in HOXA11 genes may contribute to the risk of cryptorchidism. In this study, mutation analysis was performe...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12790
更新日期:2018-02-01 00:00:00
abstract::A cryoprotectant-free method was successfully used for rapid freezing of goat epididymal spermatozoa. Lowering sperm volume may increase the temperature exchange rate and improve the freezing output of spermatozoa. The aim of this study was to compare two different packaging types [0.25 ml French straws (FS) and 96-we...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12384
更新日期:2015-12-01 00:00:00
abstract::The acrosome reaction of human spermatozoa was induced by changes of temperature. Spermatozoa were collected from fertile donors and a patient group, and selected by the "swim-up" method. The spermatozoa were treated in two different ways: Protocol I: 24 hours at room temperature followed by additional incubation at 3...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1991.tb02537.x
更新日期:1991-05-01 00:00:00
abstract::This study aimed to evaluate the effect of N-acetyl cysteine on the male reproductive system and consensus and classification of data found from previous studies. It is undeniable that N-acetyl cysteine as a powerful antioxidant compound can medicate many diseases such as cardiovascular, kidney, liver and reproductive...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/and.13898
更新日期:2021-02-01 00:00:00
abstract::Testicular sperm extraction is a common surgical procedure to retrieve spermatozoa from men with azoospermia which may affect the tissue and cause hypogonadism (Human Reproduction Update, 24, 2018, 442). We aimed to evaluate the acute effect of micro-TESE on blood total testosterone (TT) and luteinising hormone (LH) l...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.13655
更新日期:2020-08-01 00:00:00
abstract::In order to elucidate the ultrastructural characteristics of the precursor cell of Leydig cells and its subsequent differentiation, testicular biopsies from two patients with hypogonadotropic hypogonadism, obtained prior to and after treatment with HCG and HMG, were studied in comparison with those of prepubertal, pub...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1979.tb02187.x
更新日期:1979-05-01 00:00:00
abstract::Tubular hyalinization was studied both light and electron microscopically in 22 cases. 8 of them were found in undescended testes and 5 cases represented possible intermediary forms with only partial hyalinization of the tubules. In these testes spermatogenic arrest, hypospermatogenesis, only Sertoli cells and areas w...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1986.tb01746.x
更新日期:1986-01-01 00:00:00
abstract::Some cases of testicular trauma cause infertility especially when the injured testes are not removed. However, only a few long-term follow-up studies investigating endocrinological and semen parameters in patients who had testicular trauma have been conducted. Herein, we report an interesting case of a patient who spo...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12089
更新日期:2014-05-01 00:00:00
abstract::Previous studies have confirmed that spermatogenesis in homozygous Immp2l mutant male mice was normal at the age of 6 months, but was significantly abnormal at the age of 13 months. Meanwhile, oxidative stress is reported to be involved in spermatogenic impairment in old mutant mice. However, it is unclear whether ant...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12848
更新日期:2018-03-01 00:00:00
abstract::Over the past decades, there is an increasing number of association studies of telomere length (TL) and the risk and recurrence of prostate cancer (PCa), but the results are inconsistent. Hence, we identify the relevant studies published in English on or before 10 January 2019 conducting a literature review in the ele...
journal_title:Andrologia
pub_type: 杂志文章,meta分析
doi:10.1111/and.13304
更新日期:2019-08-01 00:00:00
abstract::To assess the protective effects of Eugenia jambolana extract (EJE) or N-acetyl cysteine (NAC) on testis, cisplatin (CIS, 5 mg kg(-1) bw, single dose) was administered either alone or along with EJE (25 mg kg(-1) bw, alternate day) or NAC (150 mg kg(-1) bw, Day 1 and 4) for 7 days. Significant alterations in serum LH,...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12247
更新日期:2015-03-01 00:00:00
abstract::The aim of our pilot study was to check whether the differences in IgA secretory component (SC) sialylation are associated with leucocytospermia. In normozoospermic and leucocytospermic seminal plasmas, 78-kDa and 63-kDa SC immunoreactive bands were observed. The SC sialylation was analysed by lectin blotting, using s...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/and.12213
更新日期:2014-12-01 00:00:00
abstract::In fertile men, the majority of epididymal spermatozoa acquire the potential to fertilize (assessed with sperm function assays) on passage into the corpus and cauda regions of the epididymis. Although secretions of the epididymal epithelium are clearly important for sperm maturation and survival, their role in this pr...
journal_title:Andrologia
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0272.1998.tb01165.x
更新日期:1998-08-01 00:00:00
abstract::Searching for further characteristics of sperm in the diagnosis of male infertility it was examined if ejaculates with "normo-, oligo-, astheno-, terato-" and "azoospermia" showed typical modifications in their triglyceride- and phospholipid contents. The examination was conveyed in spermatozoa and seminal plasma. In ...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1984.tb00233.x
更新日期:1984-01-01 00:00:00
abstract::There is growing concern that abnormalities in male reproductive health are becoming more frequent. The most fundamental change has been the striking decline in sperm counts and semen quality. The effect of maternal exposure of rats to the oestrogenic environmental substance p-nonylphenol (p-NP) was determined in this...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1046/j.1439-0272.1999.00246.x
更新日期:1999-03-01 00:00:00
abstract::The hypothalamic-pituitary-testicular axis and the regulation of prolactin secretion were investigated in eleven male renal transplant recipients. Mean serum levels of testosterone and estrone were normal, whereas those of androstenedione and estradiol were low. Mean basal luteinizing hormone (LH) levels were slightly...
journal_title:Andrologia
pub_type: 杂志文章
doi:10.1111/j.1439-0272.1988.tb00670.x
更新日期:1988-03-01 00:00:00