Direct evidence that radiation induced micronuclei of early embryos require a mitosis for expression.

Abstract:

:The naturally synchronous development of early mouse embryos was exploited to address the question, whether micronuclei require a mitosis for expression or whether they can be expressed in the same cell cycle, in which exposure to X-rays or caffeine took place. Experiments with 2-cell and with 4-cell embryos showed that micronuclei are expressed only if a mitosis is completed. There was no indication, even after doses up to 20 Gy, that micronuclei can be expressed before the mitosis was reached, which followed exposure. Furthermore, no nuclear fragmentation pointing to apoptosis could be detected in the cycle, in which cells were exposed. The same results were obtained when caffeine (5 mM) was used as micronucleus inducing agent.

journal_name

Radiat Environ Biophys

authors

Müller WU,Schlusen I,Streffer C

doi

10.1007/BF01219345

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

117-22

issue

2

eissn

0301-634X

issn

1432-2099

journal_volume

30

pub_type

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