Manipulation of the bluetongue virus tubules for immunogen delivery.


:A multidisplay vaccine delivery system has been developed that is nonreplicating and has a protein-based particulate structure. The structure is composed of helical tubules comprising multiple copies of a single nonstructural (NS) protein 1 of bluetongue virus. The helical assemblies present the C terminus of the protein on the surface of the tubules, thereby displaying appended residues in regular and repeating arrays. The NS1 protein has been manipulated to carry chosen immunogens at this C terminus, such that many thousands of copies of the foreign immunogen are displayed on the surface of the tubules. The display system can accommodate more than 500 amino acid residues in length without perturbing the basic tubular structure. Many immunogens have been displayed and tested for immunogenicity and have been shown to stimulate both humoral and cellular responses. NS1 tubules represent a safe vaccine-delivery system with great potential in the vaccine arena.


Future Microbiol


Future microbiology


Murphy A,Roy P




Has Abstract


2008-06-01 00:00:00












  • N-linked glycosylation in bacteria: an unexpected application.

    abstract::Traditionally, glycoproteins have been considered the exclusive property of eukaryotes and archaea, but it is now evident that glycoproteins are found in all domains of life. In recent years N-linked glycosylation among some epsilon-proteobacteria has emerged as a new and exciting research area and represents a useful...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Langdon RH,Cuccui J,Wren BW

    更新日期:2009-05-01 00:00:00

  • Better than a pound of cure: preventing the development of multidrug-resistant tuberculosis.

    abstract::Diagnostic and treatment delays contribute to increased death and disability among the 490,000 adults and children who develop multidrug-resistant (MDR) tuberculosis every year. Since the treatment of MDR tuberculosis is complex, costly and often toxic, tuberculosis control programs should prioritize strategies to pre...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Byrne AL,Fox GJ,Marais BJ

    更新日期:2018-04-01 00:00:00

  • Measles virus hemagglutinin epitopes are potential hotspots for crossreactions with immunodeficiency-related proteins.

    abstract:AIMS:Measles virus (MV) infection induces a protective immunity that is accompanied by a transient pathologic suppression of the immune system. This immunologic paradox remains unexplained in spite of the numerous hypotheses that have been advanced (i.e., cytokine production, soluble immunosuppressive factor, cell cycl...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Kanduc D

    更新日期:2015-01-01 00:00:00

  • Cif type III effector protein: a smart hijacker of the host cell cycle.

    abstract::During coevolution with their hosts, bacteria have developed functions that allow them to interfere with the mechanisms controlling the proliferation of eukaryotic cells. Cycle inhibiting factor (Cif) is one of these cyclomodulins, the family of bacterial effectors that interfere with the host cell cycle. Acquired ear...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Samba-Louaka A,Taieb F,Nougayrède JP,Oswald E

    更新日期:2009-09-01 00:00:00

  • Methods for culturing anaerobes from human specimen.

    abstract::Anaerobes represent the dominating population in the human gut microbiota and play a key role in gut homeostasis. In addition, several anaerobes are now considered as probiotics and they remain essential to several processes in the field of biotechnology. With the implementation of MALDI-TOF MS in routine laboratories...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Guilhot E,Khelaifia S,La Scola B,Raoult D,Dubourg G

    更新日期:2018-03-01 00:00:00

  • Potential impact of removing metronidazole from treatment armamentarium of mild acute Clostridioides difficile infection.

    abstract::Aim: Recent guidelines recommended removing metronidazole as a therapeutic option for Clostridioides difficile infections (CDI). However, superiority of vancomycin over metronidazole in mild CDI is not established and use of vancomycin might lead to emergence of vancomycin-resistant enterococci (VRE). Patients & metho...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Zilberman-Itskovich S,Youngster I,Lazarovitch T,Bondarenco M,Toledano L,Kachlon Y,Mengesha B,Strul N,Zaidenstein R,Marchaim D

    更新日期:2019-11-01 00:00:00

  • Preclinical efficacy of a virus-like particle-based vaccine against avian influenza H5N1.

    abstract::Evaluation of: Kang S-M, Yoo D-G, Lipatov AS et al.: Induction of long-term protective immune responses by influenza H5N1 virus-like particles. PLoS ONE 4, e4667 (2009). This article describes a long-term protective effect of a vaccine candidate against avian influenza H5N1 that is based on A/Viet Nam1203/04 proteins ...

    journal_title:Future microbiology

    pub_type: 评论,杂志文章


    authors: Arnon R,Ben-Yedidia T

    更新日期:2009-06-01 00:00:00

  • Probiotics Clostridium butyricum and Bacillus subtilis ameliorate intestinal tumorigenesis.

    abstract:AIMS:To investigate the antitumor effects of probiotics Clostridium butyricum and Bacillus subtilis on colorectal cancer (CRC) progression. MATERIALS & METHODS:The effects of C. butyricum and B. subtilis on CRC cells were studied. Male C57BL/6 mice with 1,2-dimethylhydrazine dihydrochloride (DMH)-induced CRC were inte...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Chen ZF,Ai LY,Wang JL,Ren LL,Yu YN,Xu J,Chen HY,Yu J,Li M,Qin WX,Ma X,Shen N,Chen YX,Hong J,Fang JY

    更新日期:2015-01-01 00:00:00

  • The role of bacteriophages in periodontal health and disease.

    abstract::The human periodontium health is commonly compromised by chronic inflammatory conditions and has become a major public health concern. Dental plaque, the precursor of periodontal disease, is a complex biofilm consisting mainly of bacteria, but also archaea, protozoa, fungi and viruses. Viruses that specifically infect...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Pinto G,Silva MD,Peddey M,Sillankorva S,Azeredo J

    更新日期:2016-10-01 00:00:00

  • How Cryptococcus interacts with the blood-brain barrier.

    abstract::Cryptococcus demonstrates predilection for invasion of the brain, but the mechanism by which Cryptococcus crosses the blood-brain barrier (BBB) to cause brain invasion is largely unknown. In order for Cryptococcus to cross the BBB, there must be a way to either cross human brain microvascular endothelial cells, which ...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Tseng HK,Huang TY,Wu AY,Chen HH,Liu CP,Jong A

    更新日期:2015-01-01 00:00:00

  • Antibiotic-associated diarrhea: epidemiology, trends and treatment.

    abstract::A common complication of antibiotic use is the development of gastrointestinal disease. This complication ranges from mild diarrhea to pseudomembranous colitis. Outbreaks of antibiotic-associated diarrhea (AAD) may also occur in healthcare settings, usually caused by Clostridium difficile. AAD typically occurs in 5-35...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: McFarland LV

    更新日期:2008-10-01 00:00:00

  • Use of the plaque assay for testing the antibiotic susceptibility of intracellular bacteria.

    abstract::The plaque assay was first described for titration of bacterial inoculums and clonal isolation, and was later adapted for testing antibiotics susceptibility and to study virulence factors and motility of bacteria. Over time, the sensitivity and reproducibility of the technique has been improved. Usually, the number of...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Edouard S,Raoult D

    更新日期:2013-10-01 00:00:00

  • Endothelial cells and CMV dissemination.

    abstract::Using the murine CMV animal model and the well-established model of Cre-lox-P-mediated green-fluorescence tagging of endothelial cell (EC)-derived mouse CMV to quantify the role of infected ECs in transplantation-associated CMV dissemination (in mice expressing Cre recombinase under the control of either the Tie2 or t...

    journal_title:Future microbiology

    pub_type: 评论,杂志文章


    authors: Gerna G

    更新日期:2012-04-01 00:00:00

  • Update on the epidemiological typing methods for Acinetobacter baumannii.

    abstract::The outstanding ability of Acinetobacter baumannii to cause outbreaks and acquire multidrug resistance motivated the development of a plethora of typing techniques, which can help infection preventionists and hospital epidemiologists to more efficiently implement intervention controls. Nowadays, the world is witnessin...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Rafei R,Osman M,Dabboussi F,Hamze M

    更新日期:2019-08-01 00:00:00

  • Letermovir for prophylaxis of cytomegalovirus manifestations in adult allogeneic hematopoietic stem cell transplant recipients.

    abstract::Cytomegalovirus (CMV) manifestations remain important complications after allogeneic hematopoietic stem cell transplantation (HSCT), even in the current era. Unfortunately, available anti-CMV agents, mainly viral polymerase inhibitors, have a substantial risk of myelosuppression and nephrotoxicity. Letermovir, a novel...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Hecke SV,Calcoen B,Lagrou K,Maertens J

    更新日期:2019-02-01 00:00:00

  • Comparison of antibiotic susceptibility of old and current Serratia.

    abstract:AIMS:We explored changes in antibiotic susceptibility of Serratia marcescens in the last 50 years by comparing isolates collected between 1945 and 1950, and current isolates. MATERIALS & METHODS:Isolates were divided into three groups: environmental, clinical and 'old'. Susceptibility was determined by microdilution. ...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Fusté E,Galisteo GJ,Jover L,Vinuesa T,Villa TG,Viñas M

    更新日期:2012-06-01 00:00:00

  • MAPK cell-cycle regulation in Saccharomyces cerevisiae and Candida albicans.

    abstract::The cell cycle is the sequential set of events that living cells undergo in order to duplicate. This process must be tightly regulated as alterations may lead to diseases such as cancer. The molecular events that control the cell cycle are directional and involve regulatory molecules such as cyclins and cyclin-depende...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Correia I,Alonso-Monge R,Pla J

    更新日期:2010-07-01 00:00:00

  • New perspectives in the management of Pseudomonas aeruginosa infections.

    abstract::Infections with Pseudomonas aeruginosa are a major health problem, especially for immune-compromised and cystic fibrosis patients, owing to the particular drug resistance of the microorganism. The aim of this review is to provide recent insights into strategies under investigation for prevention and therapy of these i...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Savoia D

    更新日期:2014-01-01 00:00:00

  • First fungemia case due to environmental yeast Wickerhamomyces myanmarensis: detection by multiplex qPCR and antifungal susceptibility.

    abstract:AIM:Presenting the first clinical case of Wickerhamomyces myanmarensis. PATIENTS & METHODS:Yeast cells were isolated from blood and central venous catheter of a 5.5-year-old male subject. API 20C AUX, MALDI-TOF MS, ITS and LSU rDNA sequencing, and our qPCR assay were used for identification and the MIC values were det...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Arastehfar A,Bakhtiari M,Daneshnia F,Fang W,Sadati SK,Al-Hatmi AM,Groenewald M,Sharifi-Mehr H,Liao W,Pan W,Zomorodian K,Hagen F,Boekhout T

    更新日期:2019-03-01 00:00:00

  • Potential for adaptation overrides cost of resistance.

    abstract:AIM:To investigate the cost of antibiotic resistance versus the potential for resistant clones to adapt in maintaining polymorphism for resistance. MATERIALS & METHODS:Experimental evolution of Escherichia coli carrying different resistance alleles was performed under an environment devoid of antibiotics and evolution...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Moura de Sousa J,Sousa A,Bourgard C,Gordo I

    更新日期:2015-01-01 00:00:00

  • Group A Streptococcus encounters with host macrophages.

    abstract::Group A Streptococcus (GAS) is a leading human bacterial pathogen with diverse clinical manifestations. Macrophages constitute a critical first line of host defense against GAS infection, using numerous surface and intracellular receptors such as Toll-like receptors and inflammasomes for pathogen recognition and activ...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Valderrama JA,Nizet V

    更新日期:2018-01-01 00:00:00

  • Uncivil engineers: Chlamydia, Salmonella and Shigella alter cytoskeleton architecture to invade epithelial cells.

    abstract::The obligate intracellular bacterial pathogen Chlamydia trachomatis is a major cause of blindness and sexually transmitted diseases. Like the enteric pathogens Salmonella and Shigella, Chlamydia injects effector proteins into epithelial cells to initiate extensive remodeling of the actin cytoskeleton at the bacterial ...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Dunn JD,Valdivia RH

    更新日期:2010-08-01 00:00:00

  • SAMHD1: a novel antiviral factor in intrinsic immunity.

    abstract::Some intracellular/membranous factors exert intrinsic immunity against viral pathogens. Most recently, SAMHD1 has been shown to be one of these factors. SAMHD1 is a nucleus-localized protein, and mutations in the gene are associated with Aicardi-Goutières syndrome. As a triphosphohydrolase, it depletes the intracellul...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Chen Z,Zhang L,Ying S

    更新日期:2012-09-01 00:00:00

  • Fusarium oxysporum is an onychomycosis etiopathogenic agent.

    abstract:AIM:To evaluate and characterize the etiopathogenesis of the fusarial onychomycosis in an ex vivo study through fragments of sterile human nail, without the addition of any nutritional source. MATERIALS & METHODS:The infection and invasion of Fusarium oxysporum in the nail were evaluated by scanning electron microscop...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Veiga FF,de Castro-Hoshino LV,Sato F,Bombassaro A,Vicente VA,Mendes V,Baesso ML,Negri M,Svidzinski TI

    更新日期:2018-12-01 00:00:00

  • New insights into Prevotella diversity and medical microbiology.

    abstract::In light of recent studies based on cultivation-independent methods, it appears that the diversity of Prevotella in human microbiota is greater than was previously assumed from cultivation-based studies, and that the implication of these bacteria in several human diseases was unrecognized. While some Prevotella taxa w...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Alauzet C,Marchandin H,Lozniewski A

    更新日期:2010-11-01 00:00:00

  • Interview with Professor Mark Wilcox.

    abstract::Mark Wilcox speaks to Georgia Patey, Commissioning Editor: Professor Mark Wilcox is a Consultant Microbiologist and Head of Microbiology at the Leeds Teaching Hospitals (Leeds, UK), the Professor of Medical Microbiology at the University of Leeds (Leeds, UK), and is the Lead on Clostridium difficile and the Head of th...

    journal_title:Future microbiology

    pub_type: 面试


    authors: Wilcox M

    更新日期:2016-08-01 00:00:00

  • Hepatitis E in developed countries: current status and future perspectives.

    abstract::Hepatitis E virus (HEV) was for many years thought to be found almost exclusively in developing countries, where it is a major health issue. Recent studies have shown that HEV causes acute and chronic infection in developed countries. In these geographical settings, HEV is primarily a porcine zoonosis caused by genoty...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Dalton HR,Kamar N,Izopet J

    更新日期:2014-01-01 00:00:00

  • The ferric uptake regulator of Helicobacter pylori: a critical player in the battle for iron and colonization of the stomach.

    abstract::Helicobacter pylori is arguably one of the most successful pathogens; it colonizes the stomachs of more than half of the human population. Colonization and persistence in such an inhospitable niche requires the presence of exquisite adaptive mechanisms. One of the proteins that contributes significantly to the remarka...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Pich OQ,Merrell DS

    更新日期:2013-06-01 00:00:00

  • Organism-wide studies into pathogenicity and morphogenesis in Talaromyces marneffei.

    abstract::Organism-wide approaches examining the genetic mechanisms controlling growth and proliferation have proven to be a powerful tool in the study of pathogenic fungi. For many fungal pathogens techniques to study transcription and protein expression are particularly useful, and offer insights into infection processes by t...

    journal_title:Future microbiology

    pub_type: 杂志文章,评审


    authors: Weerasinghe H,Payne M,Beard S,Andrianopoulos A

    更新日期:2016-01-01 00:00:00

  • Clinical metagenomics for the management of hospital- and healthcare-acquired pneumonia.

    abstract::The increasing burden of multidrug-resistant bacteria affects the management of several infections. In order to prescribe adequate antibiotics, clinicians facing severe infections such as hospital-acquired pneumonia (HAP) need to promptly identify the pathogens and know their antibiotic susceptibility profiles (AST), ...

    journal_title:Future microbiology

    pub_type: 杂志文章


    authors: Ruppé E,Baud D,Schicklin S,Guigon G,Schrenzel J

    更新日期:2016-01-01 00:00:00