Abstract:
:Spiders have evolved a complex system of silk producing glands. Each of the glands produces silk with strength and elasticity tailored to its biological purpose. Sequence analysis of the major ampullate silk reveals four highly conserved concatenated blocks of amino acids: (GA) n , A n , GPGXX, and GGX. While the GPGXX motif, which has been hypothesized to be responsible for the extensibility of the fiber, displays natural variation in its precise sequence arrangement and content, correlating these differences with particular fiber properties has been difficult. Three genetic constructs based on the Argiope aurantia sequence were engineered to progressively increase the number of GPGXX repeats in a head-to-tail assembly prior to interruption by another motif. Circular dichroism and Fourier transform infrared spectroscopy of synthetic spider silk spin dopes show secondary structures that correspond to an increase in the repeat number of GPGXX regions and an increase in the extensibility of synthetically spun recombinant fibers.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Brooks AE,Stricker SM,Joshi SB,Kamerzell TJ,Middaugh CR,Lewis RVdoi
10.1021/bm701124psubject
Has Abstractpub_date
2008-06-01 00:00:00pages
1506-10issue
6eissn
1525-7797issn
1526-4602journal_volume
9pub_type
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