The apparent rate constant for the dissociation of force generating myosin crossbridges from actin decreases during Ca2+ activation of skinned muscle fibres.

abstract：:Rotary-shadowed filamin molecules appear as long, highly flexible rods curved into a variety of configurations. The particles observed were 2.7 nm wide but had contour lengths of either 98 nm or 193 nm. The longer particles are probably end-to-end dimers of the shorter but it is not clear how many polypeptide chains t...

journal_title：Journal of muscle research and cell motility

authors： Castellani L,Offer G,Elliott A,O'Brien EJ

更新日期：1981-06-01 00:00:00

abstract：:Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...

journal_title：Journal of muscle research and cell motility

authors： Goldfine SM,Einheber S,Fischman DA

更新日期：1991-04-01 00:00:00

abstract：:Myosin heavy chain kinase activity was identified in the high speed supernate of lysed Dictyostelium amoebae and was precipitated by 30-50% ammonium sulphate. In low ionic strength buffer, the activity bound tightly to a Cibacron Blue Sepharose column and eluted as a single peak with 1.0 M NaCl. Gel filtration chromat...

journal_title：Journal of muscle research and cell motility

authors： Kuczmarski ER

更新日期：1986-12-01 00:00:00

abstract：:The time course of shortening at zero load was studied by the slack test method during tetanic contractions in isolated, single, slow muscle fibres of the crayfish. In 28 of 32 shortenings (from 14 different fibres) a biphasic shortening was seen, which consisted of an initial high-velocity phase lasting 3.3-20.8 ms a...

journal_title：Journal of muscle research and cell motility

authors： Tameyasu T

更新日期：1992-12-01 00:00:00

abstract：:There is evidence to suggest that 8 nm calcium transport particles in the sarcoplasmic reticulum are involved in the regulation of twitch properties in adult muscles. We have studied ultrastructural characteristics of the sarcoplasmic reticulum in relation to previously defined physiological changes that take place in...

journal_title：Journal of muscle research and cell motility

authors： Gauthier GF,Hobbs AW

更新日期：1986-04-01 00:00:00

abstract：:Myosin has been purified from chicken pectoralis muscle at various stages of development, from 10 days' incubation to approximately 10 months after hatching. Embryonic myosin from the earliest stage showed a high level of ATPase activity, similar to that obtained for adult pectoralis myosin. Two-dimensional peptide ma...

journal_title：Journal of muscle research and cell motility

authors： Lowey S,Benfield PA,LeBlanc DD,Waller GS

更新日期：1983-12-01 00:00:00

abstract：:To explain observed differences in the activation dependence of force redevelopment kinetics between cardiac and skeletal muscle, two numerical models of contractile regulation by Ca2+ were investigated. Ca2+ binding and force production were each modelled as two-state processes with forward and reverse rate constants...

journal_title：Journal of muscle research and cell motility

authors： Hancock WO,Huntsman LL,Gordon AM

更新日期：1997-12-01 00:00:00

abstract：:Two procedures to inhibit Ca(2+) release designed to differentiate between local and common pool mechanisms for the Ca(2+) dependent, fast inactivation of Ca(2+) release in skeletal muscle of the frog were compared. Inhibition by voltage dependent inactivation of Ca(2+) release, without modification of the single chan...

journal_title：Journal of muscle research and cell motility

authors： Olivera JF,Pizarro G

更新日期：2010-08-01 00:00:00

abstract：:Protein kinase C (PKC) is known to play important roles in integrin mediated cell spreading. This study investigated the role of PKC during insulin mediated muscle cell spreading, which was independent of integrin alpha5. We found that PKC-alpha becomes active and localise to membrane during insulin mediated cell spre...

journal_title：Journal of muscle research and cell motility

authors： Goel HL,Dey CS

更新日期：2002-01-01 00:00:00

abstract：:This study investigated the effects of the protein kinase A (PKA) inhibitor, H-89, in mechanically-skinned muscle fibres and intact muscle fibres, in order to determine whether PKA phosphorylation is essential for normal excitation-contraction (E-C) coupling. In skinned EDL fibres of the rat, force responses to depola...

journal_title：Journal of muscle research and cell motility

authors： Blazev R,Hussain M,Bakker AJ,Head SI,Lamb GD

更新日期：2001-01-01 00:00:00

abstract：:Mechanically skinned fibre preparations from the extensor digitorum longus muscle of the rat were used to test whether a rise in myoplasmic [NH+4] in the range 2-10 mM interferes with the mechanism of excitation-contraction coupling in fast-twitch mammalian muscle. Under our conditions (pH 7.10, Mg2+ 1 mM, temperature...

journal_title：Journal of muscle research and cell motility

authors： Stephenson GM,Stephenson DG

更新日期：1996-12-01 00:00:00

abstract：:Actin filaments are major components of the cytoskeleton in eukaryotic cells and are involved in vital cellular functions such as cell motility and muscle contraction. Tmod and TM are crucial constituents of the actin filament network, making their presence indispensable in living cells. Tropomyosin (TM) is an alpha-h...

journal_title：Journal of muscle research and cell motility

authors： Colpan M,Moroz NA,Kostyukova AS

更新日期：2013-08-01 00:00:00

abstract：:Full muscle relaxation happens when [Ca2+] falls below the threshold for force activation. Several experimental models, from whole muscle organs and intact muscle down to skinned fibers, have been used to explore the cascade of kinetic events leading to mechanical relaxation. The use of single myofibrils together with...

journal_title：Journal of muscle research and cell motility

authors： Vitale G,Ferrantini C,Piroddi N,Scellini B,Pioner JM,Colombini B,Tesi C,Poggesi C

更新日期：2019-11-19 00:00:00

abstract：:In this study the spontaneous activities of prescapular lymph nodes, which were isolated from calves, goats and sheep and mesenteric lymph nodes of guinea-pigs were recorded and analysed in the frequency domain. Stretch-evoked contractions of the mesenteric lymph nodes were also recorded and their frequency characteri...

journal_title：Journal of muscle research and cell motility

authors： Tümer A,Oztürk-Demir N,Basar-Eroğlu C,Noyan A

更新日期：1983-02-01 00:00:00

abstract：:Skeletal muscle has a remarkable ability to respond to different physical stresses. Loading muscle through exercise, either anaerobic or aerobic, can lead to increases in muscle size and function while, conversely, the absence of muscle loading stimulates rapid decreases in size and function. A principal mediator of t...

journal_title：Journal of muscle research and cell motility

authors： Graham ZA,Gallagher PM,Cardozo CP

更新日期：2015-10-01 00:00:00

abstract：:We have investigated high-affinity ryanodine-binding sites in membrane preparations from representative fast-twitch and slow-twitch muscles of the rabbit and rat, as well as from human mixed muscle. Our results, obtained in high-ionic strength binding buffer, demonstrate extensive similarities in binding affinity for ...

journal_title：Journal of muscle research and cell motility

authors： Damiani E,Margreth A

更新日期：1994-04-01 00:00:00

abstract：:Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement. In this paper we report the molecular characterization of the Drosophila myosin V, identified by reverse genetics. The gene encodes a 1792-residue, 207 kDa heavy chain polypeptide which p...

journal_title：Journal of muscle research and cell motility

authors： Bonafé N,Sellers JR

更新日期：1998-02-01 00:00:00

abstract：:Myosin binding protein C (MYBPC) is a crucial component of the sarcomere and an important regulator of muscle function. While mutations in different myosin binding protein C (MYBPC) genes are well known causes of various human diseases, such as hypertrophic (HCM) and dilated (DCM) forms of cardiomyopathy as well as sk...

journal_title：Journal of muscle research and cell motility

authors： Knöll R

更新日期：2012-05-01 00:00:00

abstract：:The myosin light chains of slow muscles from different species have been examined two-dimensional gel electrophoresis. While the myosin light chain 2S of mammalian soleus muscles (rabbit, rat and guinea-pig) could not be distinguished from that of avian anterior latissimus dorsi (chicken and turkey), the 1S light chai...

journal_title：Journal of muscle research and cell motility

authors： Carraro U,dalla Libera L,Catani C

更新日期：1981-09-01 00:00:00

abstract：:We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in both ATPase and motility assays. We demonstrate that skeletal muscle myosin (S...

journal_title：Journal of muscle research and cell motility

authors： Yengo CM,Takagi Y,Sellers JR

更新日期：2012-12-01 00:00:00

abstract：:Quantitative (muscle fibre number and cross-sectional areas) and qualitative (myosin isoforms and metabolic enzyme activities) characteristics of two muscles, M. pectoralis major and M. anterior latissimus dorsi, were compared among male chickens of two lines during growth from hatching to adulthood. The lines were de...

journal_title：Journal of muscle research and cell motility

authors： Remignon H,Gardahaut MF,Marche G,Ricard FH

更新日期：1995-04-01 00:00:00

abstract：:The giant molecule titin (also called connectin) provides an elastic connection in the I-band between the Z-disk and A-band of striated muscle. This region is assembled in a tissue-specific way by extensive differential splicing events. We have raised monoclonal antibodies against the two N2-line isoforms of titin and...

journal_title：Journal of muscle research and cell motility

authors： Gautel M,Lehtonen E,Pietruschka F

更新日期：1996-08-01 00:00:00

abstract：:Single twitch muscle fibres have been isolated from various parts of the iliofibularis muscle of Xenopus laevis. After measurements of their isotonic contractile properties, myosin extraction was performed on individual fibres and the extract analysed by various forms of gel electrophoresis. In agreement with previous...

journal_title：Journal of muscle research and cell motility

authors： Lännergren J

更新日期：1987-06-01 00:00:00

abstract：:In frog semitendinosus muscle the descending limb of the length-tension curve is shifted rightward relative to that of tibialis anterior. Both the plateau right corner and the zero-force intercept are equally shifted. To investigate the reason for this shift, we compared filament lengths in the two muscles. Single fib...

journal_title：Journal of muscle research and cell motility

authors： Trombitás K,Frey L,Pollack GH

更新日期：1993-04-01 00:00:00

abstract：:In isolated myosin the reaction sequence of essential thiol groups with N-ethylmaleimide was studied using the following five approaches: kinetics of the modification reaction, effects of modification on enzyme properties, affinity chromatography of isolated subfragment-1 stemming from modified myosin, isolation of cy...

journal_title：Journal of muscle research and cell motility

authors： Kunz PA,Loth K,Watterson JG,Schaub MC

更新日期：1980-03-01 00:00:00

abstract：:Monoclonal antibodies (mAb's) have been raised against proteins in preparations of Z-discs isolated from honeybee fibrillar flight muscle. These antibodies have identified four Z-disc antigens on immunoblots of honeybee fibrillar proteins. Antibody alpha binds to the 90-100 kD protein, alpha-actinin; mAb P interacts w...

journal_title：Journal of muscle research and cell motility

authors： Saide JD,Chin-Bow S,Hogan-Sheldon J,Busquets-Turner L

更新日期：1990-04-01 00:00:00

abstract：:Melatonin (N-acetyl-5-methoxy-tryptamine) is an effective antioxidant and free radical scavenger, that has important biological effects in multiple cell types and species. Melatonin research in muscle has recently gained attention, mainly focused on its role in cells or tissue repair and regeneration after injury, due...

journal_title：Journal of muscle research and cell motility

authors： Chen B,You W,Shan T

更新日期：2020-09-01 00:00:00

abstract：:While the migration of capillary endothelial cells is believed central to the process of new blood vessel development in vivo, the biochemical basis for endothelial motility is unknown. Herein, we demonstrate that retina-derived growth factor (RDGF), a mitogen for endothelial cells (EC), stimulates the migration of mi...

journal_title：Journal of muscle research and cell motility

authors： Herman IM,D'Amore PA

更新日期：1984-12-01 00:00:00

abstract：:Chronic low-frequency electrical stimulation of rabbit fast-twitch skeletal muscle induces increased levels of two intermediate filament proteins, desmin and vimentin, during the first 3 weeks of stimulation. These increases occur over the same timecourse as reported shifts in alpha-actinin expression and increased Z-...

journal_title：Journal of muscle research and cell motility

authors： Baldi JC,Reiser PJ

更新日期：1995-12-01 00:00:00

abstract：:Reactive oxygen species (ROS) are generated in skeletal muscle both during the rest and contractile activity. Myogenic cells are equipped with antioxidant enzymes, like superoxide dismutase, catalase, glutathione peroxidase, γ-glutamylcysteine synthetase and heme oxygenase-1. These enzymes not only neutralise excessiv...

journal_title：Journal of muscle research and cell motility

authors： Kozakowska M,Pietraszek-Gremplewicz K,Jozkowicz A,Dulak J

更新日期：2015-12-01 00:00:00