Abstract:
:Actin filaments are major components of the cytoskeleton in eukaryotic cells and are involved in vital cellular functions such as cell motility and muscle contraction. Tmod and TM are crucial constituents of the actin filament network, making their presence indispensable in living cells. Tropomyosin (TM) is an alpha-helical, coiled coil protein that covers the grooves of actin filaments and stabilizes them. Actin filament length is optimized by tropomodulin (Tmod), which caps the slow growing (pointed end) of thin filaments to inhibit polymerization or depolymerization. Tmod consists of two structurally distinct regions: the N-terminal and the C-terminal domains. The N-terminal domain contains two TM-binding sites and one TM-dependent actin-binding site, whereas the C-terminal domain contains a TM-independent actin-binding site. Tmod binds to two TM molecules and at least one actin molecule during capping. The interaction of Tmod with TM is a key regulatory factor for actin filament organization. The binding efficacy of Tmod to TM is isoform-dependent. The affinities of Tmod/TM binding influence the proper localization and capping efficiency of Tmod at the pointed end of actin filaments in cells. Here we describe how a small difference in the sequence of the TM-binding sites of Tmod may result in dramatic change in localization of Tmod in muscle cells or morphology of non-muscle cells. We also suggest most promising directions to study and elucidate the role of Tmod-TM interaction in formation and maintenance of sarcomeric and cytoskeletal structure.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Colpan M,Moroz NA,Kostyukova ASdoi
10.1007/s10974-013-9349-6subject
Has Abstractpub_date
2013-08-01 00:00:00pages
247-60issue
3-4eissn
0142-4319issn
1573-2657journal_volume
34pub_type
杂志文章,评审abstract::Dihydropyridine receptors (DHPRs), ryanodine receptors (RyRs), and triadin are major components of triads of mature skeletal muscle and play crucial roles in Ca2+ release in excitation-contraction (E-C) coupling. We investigated the expression and localization of these proteins as well as intracellular Ca2+ transients...
journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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更新日期:2015-06-01 00:00:00
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
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更新日期:1982-03-01 00:00:00
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
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更新日期:1982-12-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-019-09540-y
更新日期:2020-03-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
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更新日期:1983-12-01 00:00:00
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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