Abstract：
:Cleavage of vertebrate muscle tropomyosin by bacterial Omp T produces an amino-terminally truncated product (residues 7-284). The proteolysed protein, which is resolved from the parent by electrophoresis in the presence of sodium dodecylsulphate, can be generated from a variety of striated and smooth muscle tropomyosins, including ones from mammal, bird and fish. Edman-based sequencing and mass analysis confirm that the main site of chain hydrolysis is the peptide bond between Lys 6 and Lys 7. Loss of the hexapeptide, together with the blocking group, from tropomyosin weakens its affinity for troponin. Compared to wild type, the shortened forms of rabbit skeletal tropomyosin and Atlantic salmon fast skeletal tropomyosin, as well as the unacetylated (full-length) version of the latter, all display reduced affinity for both troponin and the amino-terminal fragment of troponin-T (residues 1-158), as judged by affinity chromatography. This is consistent with the view that the amino terminal region is required for full interaction with troponin-T. Truncated tropomyosin fails to bind to F-actin at micromolar concentration, as expected. Interestingly, binding is restored by troponin in the presence of either added Ca(2+) or EGTA. Digestion of muscle tropomyosin by Omp T, which can be carried out on quantitative amounts of protein, is concluded to yield a product that has useful biochemical applications.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Goonasekara CL,Gallivan LJ,Jackman DM,Heeley DHdoi
10.1007/s10974-007-9114-9subject
Has Abstractpub_date
2007-01-01 00:00:00pages
175-82issue
2-3eissn
0142-4319issn
1573-2657journal_volume
28pub_type
杂志文章abstract：:White muscle fibres from dogfish were used to compare the energetic costs of shortening by fully active muscle and by relaxing muscle. The muscle preparation was tetanized for 0.6 s and shortened either during stimulation or during relaxation. The distance shortened was 1 mm (about 15% L0, the muscle length optimum fo...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005455505056
更新日期：1998-10-01 00:00:00
abstract：:It is generally thought that the rapid relaxation of fast muscles is facilitated by the Ca(2+) binding protein parvalbumin (Parv). Indeed superfast swimbladder (SWB) muscle of toadfish contains the largest concentration of this protein ever observed (up to 1.5 mM). At 15 degrees C toadfish perform a 100 Hz call, 400 m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-009-9175-z
更新日期：2009-01-01 00:00:00
abstract：:Trabeculae from the right ventricle of rat were chemically 'skinned' with saponin (50 micrograms ml-1 for 30 min). Caffeine (10 mM) induced a transient contracture as a consequence of Ca(2+)-release from the sarcoplasmic reticulum and subsequent activation of the myofilaments. The amplitude of the caffeine contracture...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01874151
更新日期：1992-04-01 00:00:00
abstract：:Striated muscle fine structure began to be really understood following a comprehensive survey of the matter carried out by William Bowman in the late 1830s. The publications resulting from such a study, the first of which earned for the author a precocious election as Fellow of the Royal Society, are herewith examined...
journal_title：Journal of muscle research and cell motility
pub_type： 传,历史文章,杂志文章
doi：10.1007/s10974-005-9048-z
更新日期：2006-01-01 00:00:00
abstract：:To date, there has been no report of rat TnC purification, despite the rat being an animal commonly used in physiological studies of mammalian muscle. In this study we isolated the fast and slow Troponin C isoforms from rat extensor digitorum longus (23 microg TnC/g wet weight) and soleus (17.6 microg TnC/g wet weight...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/b:jure.0000009896.78385.16
更新日期：2003-01-01 00:00:00
abstract：:Troponin T (TnT) is one of the muscle regulatory proteins and is thought to be related to unique contractile properties in diverse muscles and also to myogenesis. The expression of TnT isoforms in the chicken wing muscles was investigated by two-dimensional gel electrophoresis and immunoblotting with an antiserum agai...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1016369300532
更新日期：2001-01-01 00:00:00
abstract：:The developmental expression of nebulin was studied in embryonic chick skeletal muscle cells in vitro by means of immunofluorescence microscopy. Initially nebulin appeared homogeneously or in a punctate form in the cytoplasm, and then it was assembled into I-Z-I-like complexes containing actin and alpha-actinin but no...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738427
更新日期：1992-02-01 00:00:00
abstract：:Long-(SL > 6 microns) and short-sarcomere (SL < 4 microns) fibres were isolated from the claw muscle of the yabby (Cherax destructor) during limb regeneration and at different stages of the moult cycle. Long-sarcomere fibres were more susceptible to the changes resulting from the moult-induced atrophy compared with th...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00121136
更新日期：1995-06-01 00:00:00
abstract：:The myosin II motor from Dictyostelium discoideum has been engineered to contain single tryptophan residues at strategic locations to probe movements of switch 1 and switch 2. The tryptophan residue at W501 probes movement of the relay helix and indirectly reports on switch 2 movement. This probe suggests that there i...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-005-9004-y
更新日期：2005-01-01 00:00:00
abstract：:Isolated frog muscle fibres of four different lengths (L0, initial; L1, 15%; L2, 25%; and L3, 35% stretched vs. L0) were stimulated continuously (3 min) by suprathreshold rectangular pulses at three different frequencies (f1 = 5 Hz, f2 = 6.7 Hz and f3 = 10 Hz). Using a pair of electrodes mounted at a fixed distance, t...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:We have investigated high-affinity ryanodine-binding sites in membrane preparations from representative fast-twitch and slow-twitch muscles of the rabbit and rat, as well as from human mixed muscle. Our results, obtained in high-ionic strength binding buffer, demonstrate extensive similarities in binding affinity for ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00130421
更新日期：1994-04-01 00:00:00
abstract：:Critical illness myopathy (CIM) is associated with severe muscle atrophy and fatigue in affected patients. Apoptotic signaling is involved in atrophy and is elevated in muscles from patients with CIM. In this study we investigated underlying mechanisms of apoptosis-related pathways in muscles with different fiber type...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-015-9410-8
更新日期：2015-06-01 00:00:00
abstract：:Twitchin, also called mini-titin, is structurally related to the giant elastic protein connectin/titin, and has been found in not only striated but also smooth muscles of bivalves. Many bivalve smooth muscles such as byssus retractor muscles and the opaque part of adductor muscles are known as catch muscles that can m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-005-9030-9
更新日期：2005-01-01 00:00:00
abstract：:Reactive oxygen species (ROS) are generated in skeletal muscle both during the rest and contractile activity. Myogenic cells are equipped with antioxidant enzymes, like superoxide dismutase, catalase, glutathione peroxidase, γ-glutamylcysteine synthetase and heme oxygenase-1. These enzymes not only neutralise excessiv...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-015-9438-9
更新日期：2015-12-01 00:00:00
abstract：:In frog semitendinosus muscle the descending limb of the length-tension curve is shifted rightward relative to that of tibialis anterior. Both the plateau right corner and the zero-force intercept are equally shifted. To investigate the reason for this shift, we compared filament lengths in the two muscles. Single fib...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00115451
更新日期：1993-04-01 00:00:00
abstract：:Cells isolated from pig urinary bladders and pregnant full term human uteruses were attached longitudinally between a microforce transducer and a length displacement apparatus. Cells were stretched by applying a series of ramp-like length changes of 0.2 s duration and 10.0 microns amplitude at intervals of 15 min. Pas...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01739765
更新日期：1990-10-01 00:00:00
abstract：:The properties of spin-labelled myosin, prepared from rabbit skeletal and scallop adductor muscle, on forming a long-lived complex with ADP and vanadate (M.ADP.Vi), have been investigated. In the case of an iodoacetamide-based label attached to rabbit myosin or subfragment 1, M.ADP.Vi formation is characterized by a m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00713154
更新日期：1984-02-01 00:00:00
abstract：:The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was d...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005585807179
更新日期：1999-10-01 00:00:00
abstract：:Skeletal muscle repair requires the migration of myoblasts (activated satellite cells) both to the injury site and then within the wound to facilitate cellular alignment in preparation for differentiation, fusion and eventual healing. Along this journey, the cells encounter a range of soluble and extracellular matrix ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-013-9364-7
更新日期：2013-12-01 00:00:00
abstract：:Myosin has been purified from chicken pectoralis muscle at various stages of development, from 10 days' incubation to approximately 10 months after hatching. Embryonic myosin from the earliest stage showed a high level of ATPase activity, similar to that obtained for adult pectoralis myosin. Two-dimensional peptide ma...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712161
更新日期：1983-12-01 00:00:00
abstract：:Phosphorylation of skeletal myosin regulatory light chain (RLC) occurs in fatigue and may play a role in the inhibition of shortening velocities observed in vivo. Forces and shortening velocities were measured in permeabilized rabbit psoas fibers with either phosphorylated or dephosphorylated RLCs and in the presence ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-008-9162-9
更新日期：2009-01-01 00:00:00
abstract：:The change in orientation of myosin crossbridges in contracting muscle during sudden length changes was examined by fluorescence polarization. This study used a fluorescent ATP analogue, 1,N6-etheno-2-aza-ATP(epsilon-2-aza-ATP) as a probe. Its fluorescence is considerably enhanced upon binding with myosin and is depen...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712310
更新日期：1985-02-01 00:00:00
abstract：:Isolated fibres from various muscles were skinned mechanically in oil. From a Ca2+-loaded micropipette, local applications of Ca2+ were made. These produced a limited contraction which relaxed spontaneously. The time-course of sarcomere shortening and re-lengthening was recorded by microcinephotography. Application of...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018603032590
更新日期：1997-08-01 00:00:00
abstract：:We determined the effects of epigallocatechin-3-gallate (EGCG) and epicatechin (EC), on pump turnover and Ca2+ transport by the cardiac form of the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA). Fluorescence spectroscopy was used to directly measure SERCA ATPase activity and to measure Ca2+ uptake into cardia...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-011-9256-7
更新日期：2011-09-01 00:00:00
abstract：:Invertebrate mini-titins are members of a class of myosin-binding proteins belonging to the immunoglobulin superfamily that may have structural and/or regulatory properties. We have isolated mini-titins from three molluscan sources: the striated and smooth adductor muscles of the scallop, and the smooth catch muscles ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00141557
更新日期：1993-12-01 00:00:00
abstract：:Tropomyosin-kappa (TPM1-κ) is a newly discovered tropomyosin (TM) isoform that is exclusively expressed in the human heart and generated by an alternative splicing of the α-TM gene. We reported that TPM1-κ expression was increased in the hearts of patients with chronic dilated cardiomyopathy (DCM). To increase our und...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-010-9237-2
更新日期：2011-03-01 00:00:00
abstract：:Five monoclonal antibodies that react with different regions of myosin light chain 1 from human ventricular myocardial muscle were used to obtain information on interactions between the light chain 1 and heavy chains and generally on the tertiary structure of the light chain 1 within the myosin head. We performed West...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01766461
更新日期：1992-06-01 00:00:00
abstract：:A confocal microscope was used to investigate the reversible vacuolation of frog skeletal muscle fibres produced by the efflux and entry of glycerol (80-100 mM). The formation, development and disappearance of vacuoles was observed in the fibres by staining simultaneously with two fluorescent membrane probes, RH414 an...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00114505
更新日期：1995-08-01 00:00:00
abstract：:A balanced redox status is necessary to optimize force production in contractile apparatus, where free radicals generated by skeletal muscle are involved in some basic physiological processes like excitation-contraction coupling. Protein glutathionylation has a key role in redox regulation of proteins and signal trans...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-008-9145-x
更新日期：2008-01-01 00:00:00
abstract：:A set of five differential equations has been found which gives a satisfactory account of the isotonic and isometric properties of striated muscle. Four of these differential equations give an equally satisfactory account of the results of length-drive experiments with sinusoidal variation of length. In this case, the...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/BF00713256
更新日期：1984-10-01 00:00:00
