Abstract：
:Isolated frog muscle fibres of four different lengths (L0, initial; L1, 15%; L2, 25%; and L3, 35% stretched vs. L0) were stimulated continuously (3 min) by suprathreshold rectangular pulses at three different frequencies (f1 = 5 Hz, f2 = 6.7 Hz and f3 = 10 Hz). Using a pair of electrodes mounted at a fixed distance, the action potential at two different sites was recorded extracellularly and the propagation velocity (PV) of excitation was calculated. Throughout the trials two kinds of activity (continuous and intermittent) were observed. Two types of muscle fibres, slow (SMFs) and fast (FMFs), were distinguished depending on the rate of decrease in the PV during the period of continuous fibre activity. The duration of this period decreased with the increase of L and f. The continuous activity was followed by intermittent activity. The variety of alternations of rest periods (i.e. failure of potentials and periods of activity) characterized the pattern of SMF and FMF activity evoked by the protocol used. A coefficient of activity (k), i.e. the ratio between the number of action potentials and the number of stimulus pulses delivered for a determined time interval, was used to estimate the specificity of this pattern. A decrease in k was observed with an increase of L and f, and was differently expressed for the two fibre types. The stretch of SMFs contributed more to the shortening of the period of continuous activity and the decrease in k than did the increase of stimulation frequency. For FMFs the effect of the two factors (L and f) was identical. It is suggested that the disturbances of sarcolemmal and t-system membrane excitability during repetitive stimulation and stretch are fibre-type dependent and underlie the pattern of muscle fibre activity.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Radicheva N,Mileva K,Vydevska Msubject
Has Abstractpub_date
1998-01-01 00:00:00pages
87-94issue
1eissn
0142-4319issn
1573-2657journal_volume
19pub_type
杂志文章abstract：:Skeletal muscle has a remarkable ability to respond to different physical stresses. Loading muscle through exercise, either anaerobic or aerobic, can lead to increases in muscle size and function while, conversely, the absence of muscle loading stimulates rapid decreases in size and function. A principal mediator of t...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-015-9415-3
更新日期：2015-10-01 00:00:00
abstract：:Skeletal muscle fibers exhibit plasticity of their physiological and biochemical properties in response to the firing pattern from the innervating motor neuron. In particular, the gene expression pattern generally characteristic of a slow twitch fiber can be induced in a fast twitch fiber by chronic slow fiber type el...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-006-9080-7
更新日期：2006-01-01 00:00:00
abstract：:The present study describes transitions in myosin heavy chain expression in the extraocular muscles of rats between the ages of E17 and adult. The unique phenotype of the extraocular muscle is reflected in its fibre type composition, which is comprised by six distinct profiles, each defined by location (orbital versus...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00240928
更新日期：1996-06-01 00:00:00
abstract：:The sarcomere of striated muscle is a very efficient machine transforming chemical energy into movement. However, a wrong distribution of the generated forces may lead to self-destruction of the engine itself. A well-known example for this is eccentric contraction (elongation of the sarcomere in the activated state), ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1023/a:1026094924677
更新日期：2003-01-01 00:00:00
abstract：:Creatine kinase (CK) isoenzyme compositions of different types of single muscle fibres dissected from soleus (SOL) and extensor digitorum longus (EDL) muscles from rats were examined. CK isoenzymes were separated into cytoplasmic (CK-MM, CK-MB, CK-BB) (muscle, brain and hybrid types, respectively) and mitochondrial (m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01781172
更新日期：1991-02-01 00:00:00
abstract：:2,3-butanedione 2-monoxime (BDM, 3-30 mM) decreased twitch force of intact ventricular trabeculae isolated from 19-day embryonic chick hearts in a dose-dependent manner. The responses to BDM were rapid and reversible. In an attempt to determine the cellular basis for the inhibitory effect of BDM, experiments were carr...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00125305
更新日期：1995-02-01 00:00:00
abstract：:Skeletal muscle myofibers constantly undergo degeneration and regeneration. Histopathological features of 6 skeletal muscles (cranial tibial [CT], gastrocnemius, quadriceps femoris, triceps brachii [TB], lumbar longissimus muscles, and costal part of the diaphragm [CPD]) were compared using C57BL/10ScSn-Dmd mdx (mdx) ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-016-9452-6
更新日期：2016-10-01 00:00:00
abstract：:We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in both ATPase and motility assays. We demonstrate that skeletal muscle myosin (S...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-012-9316-7
更新日期：2012-12-01 00:00:00
abstract：:The details of the global and local structure and function of titin, a giant filamentous intrasarcomeric protein are largely undiscovered. Here we discuss a combination of bulk-solution and novel single-molecule techniques that may lend unique insights into titin's molecular dynamic, structural and mechanical characte...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1023/a:1023414624092
更新日期：2002-01-01 00:00:00
abstract：:The kinetics of smooth muscle are thought to be partially determined by the level of the expression of the 7 amino acid insert, SMB, in the myosin heavy chain, as SMB is generally expressed at higher levels in faster smooth muscle. In this study, we determined the role of this insert on shortening velocity and force r...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/b:jure.0000035879.87045.4b
更新日期：2004-01-01 00:00:00
abstract：:Guinea-pig soleus muscles were denervated and electrically stimulated for periods of 43 to 66 days. Stimuli were in 1 s bursts of 40 Hz pulses, repeated every 5 min. Other guinea-pigs were denervated for 82 days without stimulation and, in a third group, the soleus muscle was necrotized and allowed to regenerate witho...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018619503456
更新日期：1997-10-01 00:00:00
abstract：:Under muscle disuse conditions decrease of expression of MyHC of slow type, and sometimes of type IIa, as well as upregulation of expression of IIb and IId/x isoforms were observed. Through dephosphorylation and entry of NFAT molecules to the nucleus calcineurin/NFATc1 signaling pathway promotes upregulation of the sl...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-015-9428-y
更新日期：2016-04-01 00:00:00
abstract：:The width (D) and the 1,0 lattice spacing (d1,0) at various ionic compositions of mechanically skinned single fibres (from semitendinosus muscle of Rana catesbeiana) were measured at various concentrations of polyvinyl pyrrolidone (PVP K-30, Mn = 40 000) from 0 to 6% at 20 degrees C. In a standard relaxing solution (4...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01753558
更新日期：1986-06-01 00:00:00
abstract：:Ryanodine receptors (RyRs) are huge homotetrameric Ca2+ release channels localized to the sarcoplasmic reticulum. RyRs are responsible for the release of Ca2+ from the SR during excitation-contraction coupling in striated muscle cells. Recent revolutionary advancements in cryo-electron microscopy have provided a numbe...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-020-09575-6
更新日期：2020-02-10 00:00:00
abstract：:We determined the effects of epigallocatechin-3-gallate (EGCG) and epicatechin (EC), on pump turnover and Ca2+ transport by the cardiac form of the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA). Fluorescence spectroscopy was used to directly measure SERCA ATPase activity and to measure Ca2+ uptake into cardia...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-011-9256-7
更新日期：2011-09-01 00:00:00
abstract：:When skinned skeletal muscle fibres with rest sarcomere length (L = 2.5 microns) are compressed by the addition of various concentrations ([PVP]) of polyvinylpyrrolidine, the relation between the 1,0 spacing (d) of thick filament lattice and [PVP] has been known to break at d of around 35 nm, resulting in a steeper sl...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738331
更新日期：1991-10-01 00:00:00
abstract：:Because complex structural differences in adult extraocular muscles may have physiological and pathophysiological significance, the three-dimensional pattern of myosin heavy chain (MHC) isoform expression within the orbital and global layers of the muscle bellies compared with the distal tendon ends was quantitatively...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005656312518
更新日期：1999-11-01 00:00:00
abstract：:In isolated myosin the reaction sequence of essential thiol groups with N-ethylmaleimide was studied using the following five approaches: kinetics of the modification reaction, effects of modification on enzyme properties, affinity chromatography of isolated subfragment-1 stemming from modified myosin, isolation of cy...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711923
更新日期：1980-03-01 00:00:00
abstract：:Several factors have an influence on the improvement of muscle activity and motor co-ordination of mammals during post-natal development. One of them is voltage sensitive L-type calcium channel function. In striated muscles of adult mammals these channels are located in T-tubule membranes thus linking the on-coming ac...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1010305421661
更新日期：2001-01-01 00:00:00
abstract：:Myomesin-I (also known as Skelemin) is a approximately 185 kDa protein, which is highly expressed in striated muscle. It contains the prototypic class-I (type-III fibronectin) and class-II (C2-immunoglobulin) motifs. Previous studies have shown the presence of Myomesin-I at the M-line of the sarcomere, where it is tho...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-008-9137-x
更新日期：2008-01-01 00:00:00
abstract：:The effect of Ca2+ activation on the apparent rate constant governing the dissociation of force generating myosin crossbridges was studied in skinned rabbit adductor magnus fibres (fast-twitch) at 21 +/- 1 degree C. Simultaneous measurements of Ca2(+)-activated isometric force and ATPase activity were conducted in par...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01781174
更新日期：1991-02-01 00:00:00
abstract：:Full muscle relaxation happens when [Ca2+] falls below the threshold for force activation. Several experimental models, from whole muscle organs and intact muscle down to skinned fibers, have been used to explore the cascade of kinetic events leading to mechanical relaxation. The use of single myofibrils together with...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-019-09566-2
更新日期：2019-11-19 00:00:00
abstract：:Using a variety of techniques, we have demonstrated the presence of at least two fibre types in Limulus median telson levator muscle. By light and electron microscopy, large (2,156 microns 2 mean cross-sectional area) fibres have A-bands of 4.1 microns, one-half I bands of 2.15 microns and Z lines less than or equal t...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01739856
更新日期：1989-02-01 00:00:00
abstract：:Isolated fibres from various muscles were skinned mechanically in oil. From a Ca2+-loaded micropipette, local applications of Ca2+ were made. These produced a limited contraction which relaxed spontaneously. The time-course of sarcomere shortening and re-lengthening was recorded by microcinephotography. Application of...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018603032590
更新日期：1997-08-01 00:00:00
abstract：:A popular part of many athletes pre-game regime is to stretch. We examined whether a pre-injury stretching protocol could prevent acute contraction-induced injury. The in situ extensor digitorum longus (EDL) muscle of an anesthetized mouse (80 mg/kg intra-peritoneal) was used. Damage to the muscle from eccentric contr...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1013188001776
更新日期：2001-01-01 00:00:00
abstract：:Chronic low-frequency electrical stimulation of rabbit fast-twitch skeletal muscle induces increased levels of two intermediate filament proteins, desmin and vimentin, during the first 3 weeks of stimulation. These increases occur over the same timecourse as reported shifts in alpha-actinin expression and increased Z-...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00130240
更新日期：1995-12-01 00:00:00
abstract：:In frog semitendinosus muscle the descending limb of the length-tension curve is shifted rightward relative to that of tibialis anterior. Both the plateau right corner and the zero-force intercept are equally shifted. To investigate the reason for this shift, we compared filament lengths in the two muscles. Single fib...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00115451
更新日期：1993-04-01 00:00:00
abstract：:We investigated the expression of myosin subunits (myosin heavy chains) as well as light chains and the in vivo phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the adult male European hamster (Cricetus cricetus L.). Two myosin heavy chain isoenzymes could be detected under native a...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738429
更新日期：1992-02-01 00:00:00
abstract：:Effects of Mn2+ and Ca2+ on the mechanical properties of glycerinated myofibrillar bundles originating from slow S1 type muscle fibres of superficial flexor muscles of the lobster Nephrops norvegicus were investigated. Mn2+ (5-20 microM) activated the preparations in a dose-dependent manner. The sensitivity of myofibr...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005312610629
更新日期：1998-06-01 00:00:00
abstract：:White muscle fibres from dogfish were used to compare the energetic costs of shortening by fully active muscle and by relaxing muscle. The muscle preparation was tetanized for 0.6 s and shortened either during stimulation or during relaxation. The distance shortened was 1 mm (about 15% L0, the muscle length optimum fo...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005455505056
更新日期：1998-10-01 00:00:00
