Abstract：
:Critical illness myopathy (CIM) is associated with severe muscle atrophy and fatigue in affected patients. Apoptotic signaling is involved in atrophy and is elevated in muscles from patients with CIM. In this study we investigated underlying mechanisms of apoptosis-related pathways in muscles with different fiber type composition in a rat model of CIM using denervation and glucocorticoid administration (denervation and steroid-induced myopathy, DSIM). Soleus and tibialis anterior (TA) muscles showed severe muscle atrophy (40-60% of control muscle weight) and significant apoptosis in interstitial as well as myofiber nuclei that was similar between the two muscles with DSIM. Caspase-3 and -8 activities, but not caspase-9 and -12, were elevated in TA and not in soleus muscle, while the caspase-independent proteins endonuclease G (EndoG) and apoptosis inducing factor (AIF) were not changed in abundance nor differentially localized in either muscle. Anti-apoptotic proteins HSP70, -27, and apoptosis repressor with a caspase recruitment domain (ARC) were elevated in soleus compared to TA muscle and ARC was significantly decreased with induction of DSIM in soleus. Results indicate that apoptosis is a significant process associated with DSIM in both soleus and TA muscles, and that apoptosis-associated processes are differentially regulated in muscles of different function and fiber type undergoing atrophy due to DSIM. We conclude that interventions combating apoptosis with CIM may need to be directed towards inhibiting caspase-dependent as well as -independent mechanisms to be able to affect muscles of all fiber types.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Barnes BT,Confides AL,Rich MM,Dupont-Versteegden EEdoi
10.1007/s10974-015-9410-8subject
Has Abstractpub_date
2015-06-01 00:00:00pages
243-53issue
3eissn
0142-4319issn
1573-2657journal_volume
36pub_type
杂志文章abstract：:Actin filaments are major components of the cytoskeleton in eukaryotic cells and are involved in vital cellular functions such as cell motility and muscle contraction. Tmod and TM are crucial constituents of the actin filament network, making their presence indispensable in living cells. Tropomyosin (TM) is an alpha-h...
journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
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journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
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journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
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journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-005-9012-y
更新日期：2005-01-01 00:00:00
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journal_title：Journal of muscle research and cell motility
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doi：10.1007/s10974-016-9441-9
更新日期：2016-04-01 00:00:00
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journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712310
更新日期：1985-02-01 00:00:00
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journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01753413
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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更新日期：2002-01-01 00:00:00
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journal_title：Journal of muscle research and cell motility
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journal_title：Journal of muscle research and cell motility
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