Mechanical properties of mammalian single smooth muscle cells. III. Passive properties of pig detrusor and human a terme uterus cells.

abstract：:Heat rate and tension were measured during the steady state of isovelocity shortening in frog sartorius muscles at two speeds (7.5 and 2.0 mm s-1) through two ranges of the length-tension curve: 1.11 to 1.01 L0 and 1.01 to 0.91 L0. Both heat rate and mechanical power were higher at short length than at long length. Th...

journal_title：Journal of muscle research and cell motility

authors： Gilbert SH

更新日期：1986-04-01 00:00:00

abstract：:Phosphorylation of skeletal myosin regulatory light chain (RLC) occurs in fatigue and may play a role in the inhibition of shortening velocities observed in vivo. Forces and shortening velocities were measured in permeabilized rabbit psoas fibers with either phosphorylated or dephosphorylated RLCs and in the presence ...

journal_title：Journal of muscle research and cell motility

authors： Stewart M,Franks-Skiba K,Cooke R

更新日期：2009-01-01 00:00:00

abstract：:We investigated the regulatory roles of MgADP and free Ca2+ in isometric tension development in skinned bovine cardiac muscle. We found that, in the relaxed state without free Ca2+, MgADP elicited a sigmoidal increase in active tension, as is the case in skeletal muscle (ADP-contraction). The critical MgADP concentrat...

journal_title：Journal of muscle research and cell motility

authors： Fukuda N,Fujita H,Fujita T,Ishiwata S

更新日期：1998-11-01 00:00:00

abstract：:Purified antibodies to the thick filament accessory proteins, C-protein, X-protein and H-protein, have been used to label fibres of three rabbit muscles, psoas (containing mainly fast white fibres), soleus (containing mainly slow red fibres) and plantaris (a muscle of mixed fibre type) and their location has been exam...

journal_title：Journal of muscle research and cell motility

authors： Bennett P,Craig R,Starr R,Offer G

更新日期：1986-12-01 00:00:00

abstract：:To date, there has been no report of rat TnC purification, despite the rat being an animal commonly used in physiological studies of mammalian muscle. In this study we isolated the fast and slow Troponin C isoforms from rat extensor digitorum longus (23 microg TnC/g wet weight) and soleus (17.6 microg TnC/g wet weight...

journal_title：Journal of muscle research and cell motility

authors： O'Connell B,Stephenson GM

更新日期：2003-01-01 00:00:00

abstract：:Several factors have an influence on the improvement of muscle activity and motor co-ordination of mammals during post-natal development. One of them is voltage sensitive L-type calcium channel function. In striated muscles of adult mammals these channels are located in T-tubule membranes thus linking the on-coming ac...

journal_title：Journal of muscle research and cell motility

authors： Mänttäri S,Pyörnilä A,Harjula R,Järvilehto M

更新日期：2001-01-01 00:00:00

abstract：:Cardiac muscle M-band structures in several mammals (guinea pig, rabbit, rat and cow) and also from three teleosts (plaice, carp and roach), have been studied using electron microscopy and image processing. Axial structure seen in negatively stained isolated myofibrils or negatively stained cryo-sections shows the pre...

journal_title：Journal of muscle research and cell motility

authors： Pask HT,Jones KL,Luther PK,Squire JM

更新日期：1994-12-01 00:00:00

abstract：:Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement. In this paper we report the molecular characterization of the Drosophila myosin V, identified by reverse genetics. The gene encodes a 1792-residue, 207 kDa heavy chain polypeptide which p...

journal_title：Journal of muscle research and cell motility

authors： Bonafé N,Sellers JR

更新日期：1998-02-01 00:00:00

abstract：:Creatine kinase (CK) isoenzyme compositions of different types of single muscle fibres dissected from soleus (SOL) and extensor digitorum longus (EDL) muscles from rats were examined. CK isoenzymes were separated into cytoplasmic (CK-MM, CK-MB, CK-BB) (muscle, brain and hybrid types, respectively) and mitochondrial (m...

journal_title：Journal of muscle research and cell motility

authors： Yamashita K,Yoshioka T

更新日期：1991-02-01 00:00:00

abstract：:This study investigated the effects of the protein kinase A (PKA) inhibitor, H-89, in mechanically-skinned muscle fibres and intact muscle fibres, in order to determine whether PKA phosphorylation is essential for normal excitation-contraction (E-C) coupling. In skinned EDL fibres of the rat, force responses to depola...

journal_title：Journal of muscle research and cell motility

authors： Blazev R,Hussain M,Bakker AJ,Head SI,Lamb GD

更新日期：2001-01-01 00:00:00

abstract：:The cellular function of the giant protein titin in striated muscle is a major focus of scientific attention. Particularly, its role in passive mechanics has been extensively investigated. In strong contrast, the structural details of this filament are very poorly understood. To date, only a handful of atomic models f...

journal_title：Journal of muscle research and cell motility

authors： Marino M,Svergun DI,Kreplak L,Konarev PV,Maco B,Labeit D,Mayans O

更新日期：2005-01-01 00:00:00

abstract：:Much has been written about the effects of aging on reproductive function, especially female fertility. Much less is known about how aging may affect the contractility of the smooth muscle within the uterus, the myometrium. The myometrium is active through a woman's entire life, not just during pregnancy. Here we will...

journal_title：Journal of muscle research and cell motility

authors： Arrowsmith S,Robinson H,Noble K,Wray S

更新日期：2012-08-01 00:00:00

abstract：:Heart disease is a leading cause of death in patients with Duchenne muscular dystrophy (DMD). Patients with DMD lack the protein dystrophin, which is widely expressed in striated muscle. In skeletal muscle, the loss of dystrophin results in dramatically decreased expression of the dystrophin associated glycoprotein co...

journal_title：Journal of muscle research and cell motility

authors： Sharpe KM,Premsukh MD,Townsend D

更新日期：2013-12-01 00:00:00

abstract：:Results were obtained from contracting frog muscles by collecting high quality time-resolved, two-dimensional, X-ray diffraction patterns at the British Synchrotron Radiation Source (SERC, Daresbury, Laboratory). The structural transitions associated with isometric tension generation were recorded under conditions in ...

journal_title：Journal of muscle research and cell motility

authors： Bordas J,Diakun GP,Diaz FG,Harries JE,Lewis RA,Lowy J,Mant GR,Martin-Fernandez ML,Towns-Andrews E

更新日期：1993-06-01 00:00:00

abstract：:The characteristics of the titin filament in the vicinity of the Z-line were investigated using immunoelectron microscopy. We used monoclonal titin antibodies T-11 and T-12 on single fibres of frog skeletal muscle, and on Z-line-extracted fibres. It is well established that the I-band region of titin is elastic. We fi...

journal_title：Journal of muscle research and cell motility

authors： Trombitás K,Pollack GH

更新日期：1993-08-01 00:00:00

abstract：:Rigor complexes between actin and myosin have been shown to cause increased binding of Ca2+ to troponin C. A similar effect of force-generating crossbridges has been suggested as an explanation for the coupling between load and activation which has been observed in skeletal and cardiac muscle. The goal of this study w...

journal_title：Journal of muscle research and cell motility

authors： Fuchs F

更新日期：1985-08-01 00:00:00

abstract：:Gaps in our understanding of muscle mechanics demonstrate that the current model is incomplete. Increasingly, it appears that a role for titin in active muscle contraction might help to fill these gaps. While such a role for titin is increasingly accepted, the underlying molecular mechanisms remain unclear. The goals ...

journal_title：Journal of muscle research and cell motility

authors： Nishikawa K,Dutta S,DuVall M,Nelson B,Gage MJ,Monroy JA

更新日期：2020-03-01 00:00:00

abstract：:In this study alterations are characterized which occur, in myocardial force development morphological appearance and protein composition, during the development of cardiac hypertrophy and heart failure in monocrotaline (MCT) treated rats. The transition from cardiac hypertrophy to heart failure was studied by compari...

journal_title：Journal of muscle research and cell motility

authors： Korstjens IJ,Rouws CH,van der Laarse WJ,Van der Zee L,Stienen GJ

更新日期：2002-01-01 00:00:00

abstract：:The L-type Ca2+ channel in skeletal muscle (alpha1S) is essential for excitation-contraction (EC) coupling. Previous studies using chimeras composed of alpha1S together with alpha1C or alpha1M demonstrated the importance of the alpha1S II-III loop and of a smaller subdomain (residues 720-764; 'ECC') in skeletal EC cou...

journal_title：Journal of muscle research and cell motility

authors： Wilkens CM,Beam KG

更新日期：2003-01-01 00:00:00

abstract：:We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in both ATPase and motility assays. We demonstrate that skeletal muscle myosin (S...

journal_title：Journal of muscle research and cell motility

authors： Yengo CM,Takagi Y,Sellers JR

更新日期：2012-12-01 00:00:00

abstract：:In isolated myosin the reaction sequence of essential thiol groups with N-ethylmaleimide was studied using the following five approaches: kinetics of the modification reaction, effects of modification on enzyme properties, affinity chromatography of isolated subfragment-1 stemming from modified myosin, isolation of cy...

journal_title：Journal of muscle research and cell motility

authors： Kunz PA,Loth K,Watterson JG,Schaub MC

更新日期：1980-03-01 00:00:00

abstract：:Protein kinase C (PKC) is known to play important roles in integrin mediated cell spreading. This study investigated the role of PKC during insulin mediated muscle cell spreading, which was independent of integrin alpha5. We found that PKC-alpha becomes active and localise to membrane during insulin mediated cell spre...

journal_title：Journal of muscle research and cell motility

authors： Goel HL,Dey CS

更新日期：2002-01-01 00:00:00

abstract：:Full muscle relaxation happens when [Ca2+] falls below the threshold for force activation. Several experimental models, from whole muscle organs and intact muscle down to skinned fibers, have been used to explore the cascade of kinetic events leading to mechanical relaxation. The use of single myofibrils together with...

journal_title：Journal of muscle research and cell motility

authors： Vitale G,Ferrantini C,Piroddi N,Scellini B,Pioner JM,Colombini B,Tesi C,Poggesi C

更新日期：2019-11-19 00:00:00

abstract：:When skinned skeletal muscle fibres with rest sarcomere length (L = 2.5 microns) are compressed by the addition of various concentrations ([PVP]) of polyvinylpyrrolidine, the relation between the 1,0 spacing (d) of thick filament lattice and [PVP] has been known to break at d of around 35 nm, resulting in a steeper sl...

journal_title：Journal of muscle research and cell motility

authors： Umazume Y,Higuchi H,Takemori S

更新日期：1991-10-01 00:00:00

abstract：:Skeletal muscle has a remarkable ability to respond to different physical stresses. Loading muscle through exercise, either anaerobic or aerobic, can lead to increases in muscle size and function while, conversely, the absence of muscle loading stimulates rapid decreases in size and function. A principal mediator of t...

journal_title：Journal of muscle research and cell motility

authors： Graham ZA,Gallagher PM,Cardozo CP

更新日期：2015-10-01 00:00:00

abstract：:Scallop S1 has a region sensitive to tryptic hydrolysis not found thus far in S1s of other species, located 65K from the N-terminus as determined by SDS/polyacrylamide-gel electrophoresis. In the presence of actin the S1 heavy chain is preferentially cleaved at this site. The high-salt EDTA and calcium ATPase activiti...

journal_title：Journal of muscle research and cell motility

authors： Szentkiralyi EM

更新日期：1987-08-01 00:00:00

abstract：:Secophalloidin (SPH) is known to cause in cardiac myofibrils force without Ca(2+) (half-maximal effect approximately 2 mM) followed by irreversible loss of Ca(2+)-activated force. At maximal Ca(2+) activation, SPH increases force (half-maximal effect < 0.1 mM). We found that SPH at low concentration (0.5 mM) did not c...

journal_title：Journal of muscle research and cell motility

authors： Bukatina AE,Sieck GC,Campbell KB,Belohlavek M

更新日期：2009-01-01 00:00:00

abstract：:The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was d...

journal_title：Journal of muscle research and cell motility

authors： Prakash YS,Cody MJ,Housmans PR,Hannon JD,Sieck GC

更新日期：1999-10-01 00:00:00

abstract：:A popular part of many athletes pre-game regime is to stretch. We examined whether a pre-injury stretching protocol could prevent acute contraction-induced injury. The in situ extensor digitorum longus (EDL) muscle of an anesthetized mouse (80 mg/kg intra-peritoneal) was used. Damage to the muscle from eccentric contr...

journal_title：Journal of muscle research and cell motility

authors： Black JD,Stevens ED

更新日期：2001-01-01 00:00:00

abstract：:Five monoclonal antibodies that react with different regions of myosin light chain 1 from human ventricular myocardial muscle were used to obtain information on interactions between the light chain 1 and heavy chains and generally on the tertiary structure of the light chain 1 within the myosin head. We performed West...

journal_title：Journal of muscle research and cell motility

authors： Cornillon B,Cathiard AM,Eldin P,Anoal M,Cardinaud R,Liautard JP,Le Cunff M,Mornet D,Pons F,Leger J

更新日期：1992-06-01 00:00:00