Abstract：
:When skinned skeletal muscle fibres with rest sarcomere length (L = 2.5 microns) are compressed by the addition of various concentrations ([PVP]) of polyvinylpyrrolidine, the relation between the 1,0 spacing (d) of thick filament lattice and [PVP] has been known to break at d of around 35 nm, resulting in a steeper slope of the relationship at d greater than 35 nm. To clarify the cause of this, X-ray diffraction and crosslinking experiments were carried out. The d versus [PVP] relationship of stretched fibres (L = 3.5 microns) breaks at a d of around 29 nm. The difference between these characteristic d values, 35-29 = 6 nm, is close to the diameter of thin filaments (8 nm). The crosslinking efficiency of formaldehyde between myosin heads and thin filament surface, measured by radial stiffness increase, was found to begin to markedly increase when the relaxed fibre with rest L was compressed to a d of nearly 35 nm. In addition to these results, the d versus [PVP] relationship obtained in rigor and in high [Mg2+] (30 mM) relaxing solutions, and the crosslinking efficiency seen in high [Mg2+] solutions supported our previous hypothesis that in normal relaxing solution (containing 1 mM Mg2+) the probability of myosin heads coming into contact with the thin filament surface abruptly increases at d near 35 nm in fibres with rest L.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Umazume Y,Higuchi H,Takemori Sdoi
10.1007/BF01738331subject
Has Abstractpub_date
1991-10-01 00:00:00pages
466-71issue
5eissn
0142-4319issn
1573-2657journal_volume
12pub_type
杂志文章abstract：:The kinetics of smooth muscle are thought to be partially determined by the level of the expression of the 7 amino acid insert, SMB, in the myosin heavy chain, as SMB is generally expressed at higher levels in faster smooth muscle. In this study, we determined the role of this insert on shortening velocity and force r...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/b:jure.0000035879.87045.4b
更新日期：2004-01-01 00:00:00
abstract：:Cytokinesis is the mechanical process that allows the simplest unit of life, the cell, to divide, propagating itself. To divide, the cell converts chemical energy into mechanical energy to produce force. This process is thought to be active, due in large part to the mechanochemistry of the myosin-II ATPase. The cell's...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1023/a:1024419510314
更新日期：2002-01-01 00:00:00
abstract：:Cleavage of vertebrate muscle tropomyosin by bacterial Omp T produces an amino-terminally truncated product (residues 7-284). The proteolysed protein, which is resolved from the parent by electrophoresis in the presence of sodium dodecylsulphate, can be generated from a variety of striated and smooth muscle tropomyosi...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-007-9114-9
更新日期：2007-01-01 00:00:00
abstract：:The hypothesis that calmodulin (CaM) may act as a positive modulator of junctional SR Ca2+-release channel/ ryanodine receptor (RyRl) rests largerly on the demonstrated capacity of CaM to interact structurally and functionally with RyRl at pCa > 8 (Tripathy et al., 1995). The goal of the present [3H]-ryanodine binding...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005635330773
更新日期：2000-01-01 00:00:00
abstract：:Myomesin-I (also known as Skelemin) is a approximately 185 kDa protein, which is highly expressed in striated muscle. It contains the prototypic class-I (type-III fibronectin) and class-II (C2-immunoglobulin) motifs. Previous studies have shown the presence of Myomesin-I at the M-line of the sarcomere, where it is tho...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-008-9137-x
更新日期：2008-01-01 00:00:00
abstract：:High speed isovelocity shortening using a servo-controlled lever was performed on isolated whole frog sartorius muscles at long lengths to ensure substantial passive tension. The tension records of unstimulated control experiments were subtracted from the tension records of fully-tetanized experiments on the same musc...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00123833
更新日期：1994-02-01 00:00:00
abstract：:The effect of changes in hydrostatic pressure (up to 10 MPa) on the maximally calcium-activated tension in glycerinated rabbit psoas fibres has been examined. The steady active tension was depressed by 0.8% per MPa pressure rise. This pressure sensitivity was enhanced by the pressure of millimolar phosphate and depres...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01739967
更新日期：1989-04-01 00:00:00
abstract：:Heart disease is a leading cause of death in patients with Duchenne muscular dystrophy (DMD). Patients with DMD lack the protein dystrophin, which is widely expressed in striated muscle. In skeletal muscle, the loss of dystrophin results in dramatically decreased expression of the dystrophin associated glycoprotein co...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-013-9362-9
更新日期：2013-12-01 00:00:00
abstract：:Two procedures to inhibit Ca(2+) release designed to differentiate between local and common pool mechanisms for the Ca(2+) dependent, fast inactivation of Ca(2+) release in skeletal muscle of the frog were compared. Inhibition by voltage dependent inactivation of Ca(2+) release, without modification of the single chan...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-010-9212-y
更新日期：2010-08-01 00:00:00
abstract：:Several mutations in the beta-myosin heavy chain gene cause hypertrophic cardiomyopathy. This study investigates (1) the in vitro velocities of translocation of fluorescently-labelled actin by beta-myosin purified from soleus muscle of 30 hypertrophic cardiomyopathy patients with seven distinct beta-myosin heavy chain...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018613907574
更新日期：1997-06-01 00:00:00
abstract：:Melatonin (N-acetyl-5-methoxy-tryptamine) is an effective antioxidant and free radical scavenger, that has important biological effects in multiple cell types and species. Melatonin research in muscle has recently gained attention, mainly focused on its role in cells or tissue repair and regeneration after injury, due...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-020-09578-3
更新日期：2020-09-01 00:00:00
abstract：:Skeletal muscle tissue is mechanosensitive, as it is able to sense mechanical impacts and to translate these into biochemical signals making the tissue adapt. Among its mechanosensitive nature, skeletal muscle tissue is the largest metabolic organ of the human body. Disturbances in skeletal muscle mechanosensing and m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-017-9489-1
更新日期：2017-08-01 00:00:00
abstract：:The effects of 2,3-butanedione monoxime (BDM) on contraction characteristics were studied at 5 degrees C in single intact fibres isolated from the tibialis anterior muscle of the frog. The force-velocity relation was determined using the controlled-velocity method in either whole fibres or short fibre segments in whic...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01737994
更新日期：1992-10-01 00:00:00
abstract：:Clenbuterol is a beta2-adrenoceptor agonist primarily used for treating bronchospasm and alleviating the symptoms of chronic obstructive pulmonary disease (COPD) in the horse. In other species (rats, mice, sheep, and cattle), chronic high doses of clenbuterol (typically in the milligram per kilogram body weight range)...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1027377731137
更新日期：2003-01-01 00:00:00
abstract：:Striated muscle fine structure began to be really understood following a comprehensive survey of the matter carried out by William Bowman in the late 1830s. The publications resulting from such a study, the first of which earned for the author a precocious election as Fellow of the Royal Society, are herewith examined...
journal_title：Journal of muscle research and cell motility
pub_type： 传,历史文章,杂志文章
doi：10.1007/s10974-005-9048-z
更新日期：2006-01-01 00:00:00
abstract：:Dr. John Gergely passed away on July 26, 2013 after a long and distinguished career. His publications spanned 67 years. He founded the Department of Muscle Research in the Retina Foundation (which later became the Boston Biomedical Research Institute) and served as director for 34 years. Dr. Gergely served on the edit...
journal_title：Journal of muscle research and cell motility
pub_type： 传,历史文章,杂志文章
doi：10.1007/s10974-013-9370-9
更新日期：2013-12-01 00:00:00
abstract：:A high resolution, time efficient method is described for determining the complex modulus of activated striated muscle. In this method the applied oscillation of muscle length takes the form of pseudo-random binary noise, PRBN. As a time-domain signal, PRBN is an extension of the double step approach to include n step...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01753650
更新日期：1986-08-01 00:00:00
abstract：:The effect of urocortin (Uro), a recently discovered neuropeptide with selectivity towards corticotropin-releasing hormone type 2 receptor, was tested on whole cell currents expressed by guinea-pig gastric antrum smooth muscle cells. Uro (1 pmol/l-1 nmol/l) caused a concentration-dependent increase of Ca2+-sensitive K...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005653218639
更新日期：2000-01-01 00:00:00
abstract：:The phenotypic expression of cardiomyopathy is greatly influenced by extrinsic factors other than intrinsic genetic defects, such as environmental stress. Exercise is assumed to be an important extrinsic factor, since sudden death is sometimes seen during exercise in young patients with hypertrophic cardiomyopathy (HC...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/b:jure.0000021353.82449.9e
更新日期：2004-01-01 00:00:00
abstract：:To study the development of muscle-specific features during myogenesis, we analysed the ultrastructure and voltage-dependent currents of frog embryonic skeletal myocytes maintained in culture for 10 days. The cells were maintained under culture conditions that prevented cell division, fusion and cell contacts with neu...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005506216009
更新日期：1999-08-01 00:00:00
abstract：:Stretchin-klp is a newly described protein in Drosophila indirect flight muscles (IFM) that migrates on SDS gels as two distinct components of approximately 225 and 231 kD. Although the larger isoform is IFM specific, the smaller stretchin-klp isoform is expressed not only in IFM, but also in wild-type tissues of the ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-005-9012-y
更新日期：2005-01-01 00:00:00
abstract：:Full muscle relaxation happens when [Ca2+] falls below the threshold for force activation. Several experimental models, from whole muscle organs and intact muscle down to skinned fibers, have been used to explore the cascade of kinetic events leading to mechanical relaxation. The use of single myofibrils together with...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-019-09566-2
更新日期：2019-11-19 00:00:00
abstract：:Quantitative (muscle fibre number and cross-sectional areas) and qualitative (myosin isoforms and metabolic enzyme activities) characteristics of two muscles, M. pectoralis major and M. anterior latissimus dorsi, were compared among male chickens of two lines during growth from hatching to adulthood. The lines were de...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00122527
更新日期：1995-04-01 00:00:00
abstract：:A set of five differential equations has been found which gives a satisfactory account of the isotonic and isometric properties of striated muscle. Four of these differential equations give an equally satisfactory account of the results of length-drive experiments with sinusoidal variation of length. In this case, the...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/BF00713256
更新日期：1984-10-01 00:00:00
abstract：:The cellular function of the giant protein titin in striated muscle is a major focus of scientific attention. Particularly, its role in passive mechanics has been extensively investigated. In strong contrast, the structural details of this filament are very poorly understood. To date, only a handful of atomic models f...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-005-9017-6
更新日期：2005-01-01 00:00:00
abstract：:The trimeric protein AMP-activated protein kinase (AMPK) is an important sensor of energetic status and cellular stress, and mutations in genes encoding two of the regulatory γ subunits cause inherited disorders of either cardiac or skeletal muscle. AMPKγ2 mutations cause hypertrophic cardiomyopathy with glycogen depo...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-013-9359-4
更新日期：2013-12-01 00:00:00
abstract：:Guinea-pig soleus muscles were denervated and electrically stimulated for periods of 43 to 66 days. Stimuli were in 1 s bursts of 40 Hz pulses, repeated every 5 min. Other guinea-pigs were denervated for 82 days without stimulation and, in a third group, the soleus muscle was necrotized and allowed to regenerate witho...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018619503456
更新日期：1997-10-01 00:00:00
abstract：:Calsequestrin type-1 (CASQ1), the main sarcoplasmic reticulum (SR) Ca(2+) binding protein, plays a dual role in skeletal fibers: a) it provides a large pool of rapidly-releasable Ca(2+) during excitation-contraction (EC) coupling; and b) it modulates the activity of ryanodine receptors (RYRs), the SR Ca(2+) release ch...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-011-9277-2
更新日期：2011-12-01 00:00:00
abstract：:The organization of the principal cytoskeletal components (actin, tubulin and 10 nm filament protein) have been compared by immunofluorescence microscopy in two populations of chick heart fibroblasts, previously shown to be adapted respectively for rapid, directed migration or adhesion and growth. We find that neither...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711922
更新日期：1980-03-01 00:00:00
abstract：:The term 'microtubule dynamics' is often used to describe assembly/disassembly characteristics of this important cytoskeletal polymer. The ability to image microtubules in live Dictyostelium cells has revealed additional dynamic components, acting on the individual assembled tubules. At least two separate forces are i...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1023/a:1024446821701
更新日期：2002-01-01 00:00:00
