Abstract:
:The effect of urocortin (Uro), a recently discovered neuropeptide with selectivity towards corticotropin-releasing hormone type 2 receptor, was tested on whole cell currents expressed by guinea-pig gastric antrum smooth muscle cells. Uro (1 pmol/l-1 nmol/l) caused a concentration-dependent increase of Ca2+-sensitive K currents (I(K)) up to 500% as compared to control currents and did not affect the kinetics and voltage-dependence of inward Ca2+ currents. The I(K)-increasing effect of Uro was fully antagonized by preliminary emptying of intracellular Ca2+ stores with ryanodine and cyclopiazonic acid, as well as by bath application of selective blockers of adenylyl cyclase and cAMP-dependent protein kinase (PKA), but not by inhibitors of guanylyl cyclase, cGMP-dependent protein kinase, and protein kinase C. Comparable I(K) increase was obtained by forskolin (activator of adenylyl cyclase), Sp-cAMPS (activator of PKA), or by intracellular application of the catalytic subunit of PKA. It was concluded that Uro binds to a selective receptor in antral smooth muscle cells where it stimulates I(K) via PKA-dependent increase of Ca2+ concentration near the plasma membrane due to enhanced release from intracellular calcium stores.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Petkova-Kirova PS,Gagov HS,Duridanova DBdoi
10.1023/a:1005653218639keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
639-45issue
7eissn
0142-4319issn
1573-2657journal_volume
21pub_type
杂志文章abstract::Calsequestrin type-1 (CASQ1), the main sarcoplasmic reticulum (SR) Ca(2+) binding protein, plays a dual role in skeletal fibers: a) it provides a large pool of rapidly-releasable Ca(2+) during excitation-contraction (EC) coupling; and b) it modulates the activity of ryanodine receptors (RYRs), the SR Ca(2+) release ch...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-011-9277-2
更新日期:2011-12-01 00:00:00
abstract::Invertebrate mini-titins are members of a class of myosin-binding proteins belonging to the immunoglobulin superfamily that may have structural and/or regulatory properties. We have isolated mini-titins from three molluscan sources: the striated and smooth adductor muscles of the scallop, and the smooth catch muscles ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00141557
更新日期:1993-12-01 00:00:00
abstract::Muscle development involves a complex sequence of time and spatially regulated cellular events leading to the formation of highly specialised syncytial muscle cells displaying a common feature, the capacity of contraction. Analyses of mechanisms controlling muscle development reveals that the main steps of muscle form...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-008-9133-1
更新日期:2007-01-01 00:00:00
abstract::The effect of changes in hydrostatic pressure (up to 10 MPa) on the maximally calcium-activated tension in glycerinated rabbit psoas fibres has been examined. The steady active tension was depressed by 0.8% per MPa pressure rise. This pressure sensitivity was enhanced by the pressure of millimolar phosphate and depres...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01739967
更新日期:1989-04-01 00:00:00
abstract::While the migration of capillary endothelial cells is believed central to the process of new blood vessel development in vivo, the biochemical basis for endothelial motility is unknown. Herein, we demonstrate that retina-derived growth factor (RDGF), a mitogen for endothelial cells (EC), stimulates the migration of mi...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00713928
更新日期:1984-12-01 00:00:00
abstract::Much has been written about the effects of aging on reproductive function, especially female fertility. Much less is known about how aging may affect the contractility of the smooth muscle within the uterus, the myometrium. The myometrium is active through a woman's entire life, not just during pregnancy. Here we will...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-012-9300-2
更新日期:2012-08-01 00:00:00
abstract::In Drosophila melanogaster two high molecular weight tropomyosin isoforms, historically named heavy troponins (TnH-33 and TnH-34), are encoded by the Tm1 tropomyosin gene. They are specifically expressed in the indirect flight muscles (IFM). Their N-termini are conventional and complete tropomyosin sequences, but thei...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-005-9044-3
更新日期:2006-01-01 00:00:00
abstract::CapZ is a widely distributed and highly conserved, heterodimeric protein, that nucleates actin polymerization and binds to the barbed ends of actin filaments, preventing the addition or loss of actin monomers. CapZ interaction with actin filaments was shown to be of high affinity and decreased in the presence of PIP2....
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005489319058
更新日期:1999-02-01 00:00:00
abstract::The trimeric protein AMP-activated protein kinase (AMPK) is an important sensor of energetic status and cellular stress, and mutations in genes encoding two of the regulatory γ subunits cause inherited disorders of either cardiac or skeletal muscle. AMPKγ2 mutations cause hypertrophic cardiomyopathy with glycogen depo...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-013-9359-4
更新日期:2013-12-01 00:00:00
abstract::temporaria (0.9-2.5degrees C) were stimulated to produce a 1 s isometric tetanus at regular intervals until constant mechanical responses were attained. Various degrees of force depression ("fatigue") were produced by decreasing the contraction interval from 30 or 15 min (control to 120, 60, 30 and 15s, respectively. ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00713270
更新日期:1981-09-01 00:00:00
abstract::The five possible analogues of ATP and the three possible analogues of ADP which contain single non-bridging sulphur atoms instead of oxygen in the polyphosphate structure have been used as probes of the interaction of nucleotides with myosin and actomyosin. Evidence is presented for the requirement of an alpha, beta,...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00711928
更新日期:1980-03-01 00:00:00
abstract::The phenotypic expression of cardiomyopathy is greatly influenced by extrinsic factors other than intrinsic genetic defects, such as environmental stress. Exercise is assumed to be an important extrinsic factor, since sudden death is sometimes seen during exercise in young patients with hypertrophic cardiomyopathy (HC...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/b:jure.0000021353.82449.9e
更新日期:2004-01-01 00:00:00
abstract::The L-type Ca2+ channel in skeletal muscle (alpha1S) is essential for excitation-contraction (EC) coupling. Previous studies using chimeras composed of alpha1S together with alpha1C or alpha1M demonstrated the importance of the alpha1S II-III loop and of a smaller subdomain (residues 720-764; 'ECC') in skeletal EC cou...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1024830132118
更新日期:2003-01-01 00:00:00
abstract::Secophalloidin (SPH) is known to cause in cardiac myofibrils force without Ca(2+) (half-maximal effect approximately 2 mM) followed by irreversible loss of Ca(2+)-activated force. At maximal Ca(2+) activation, SPH increases force (half-maximal effect < 0.1 mM). We found that SPH at low concentration (0.5 mM) did not c...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-009-9188-7
更新日期:2009-01-01 00:00:00
abstract::The sarcomere of striated muscle is a very efficient machine transforming chemical energy into movement. However, a wrong distribution of the generated forces may lead to self-destruction of the engine itself. A well-known example for this is eccentric contraction (elongation of the sarcomere in the activated state), ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1023/a:1026094924677
更新日期:2003-01-01 00:00:00
abstract::Filamin binds to F-actin and influences the myosin-actin interaction. At relatively low concentrations, filamin activates actomyosin Mg2+-ATPase, whereas higher concentrations of filamin exert an inhibitory effect. Activation of ATPase activity occurs under conditions where a loose meshwork of actin filaments is prese...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00712309
更新日期:1985-02-01 00:00:00
abstract::Dr. John Gergely passed away on July 26, 2013 after a long and distinguished career. His publications spanned 67 years. He founded the Department of Muscle Research in the Retina Foundation (which later became the Boston Biomedical Research Institute) and served as director for 34 years. Dr. Gergely served on the edit...
journal_title:Journal of muscle research and cell motility
pub_type: 传,历史文章,杂志文章
doi:10.1007/s10974-013-9370-9
更新日期:2013-12-01 00:00:00
abstract::When skinned skeletal muscle fibres with rest sarcomere length (L = 2.5 microns) are compressed by the addition of various concentrations ([PVP]) of polyvinylpyrrolidine, the relation between the 1,0 spacing (d) of thick filament lattice and [PVP] has been known to break at d of around 35 nm, resulting in a steeper sl...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738331
更新日期:1991-10-01 00:00:00
abstract::We have determined the amino acid sequences of the essential light chains (ELC) and regulatory light chains (RLC) of myosin from two species of clam, Mercenaria mercenaria and Macrocallista nimbosa, using protein chemistry methods. The N-termini of all four proteins were blocked, and sequencing was carried out on vari...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738587
更新日期:1991-08-01 00:00:00
abstract::Skeletal muscle hypertrophy is a result of increased load, such as functional and stretch-overload. Activation of satellite cells and proliferation, differentiation and fusion are required for hypertrophy of overloaded skeletal muscles. On the contrary, a dramatic loss of skeletal muscle mass determines atrophy settin...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-010-9204-y
更新日期:2009-12-01 00:00:00
abstract::Tropomyosins (Tm) in humans are expressed from four distinct genes and by alternate splicing >40 different Tm polypeptide chains can be made. The functional Tm unit is a dimer of two parallel polypeptide chains and these can be assembled from identical (homodimer) or different (heterodimer) polypeptide chains provided...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-013-9353-x
更新日期:2013-08-01 00:00:00
abstract::Single twitch muscle fibres have been isolated from various parts of the iliofibularis muscle of Xenopus laevis. After measurements of their isotonic contractile properties, myosin extraction was performed on individual fibres and the extract analysed by various forms of gel electrophoresis. In agreement with previous...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01574594
更新日期:1987-06-01 00:00:00
abstract::High speed isovelocity shortening using a servo-controlled lever was performed on isolated whole frog sartorius muscles at long lengths to ensure substantial passive tension. The tension records of unstimulated control experiments were subtracted from the tension records of fully-tetanized experiments on the same musc...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00123833
更新日期:1994-02-01 00:00:00
abstract::Reactive oxygen species (ROS) are generated in skeletal muscle both during the rest and contractile activity. Myogenic cells are equipped with antioxidant enzymes, like superoxide dismutase, catalase, glutathione peroxidase, γ-glutamylcysteine synthetase and heme oxygenase-1. These enzymes not only neutralise excessiv...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-015-9438-9
更新日期:2015-12-01 00:00:00
abstract::Skeletal muscle repair requires the migration of myoblasts (activated satellite cells) both to the injury site and then within the wound to facilitate cellular alignment in preparation for differentiation, fusion and eventual healing. Along this journey, the cells encounter a range of soluble and extracellular matrix ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-013-9364-7
更新日期:2013-12-01 00:00:00
abstract::A popular part of many athletes pre-game regime is to stretch. We examined whether a pre-injury stretching protocol could prevent acute contraction-induced injury. The in situ extensor digitorum longus (EDL) muscle of an anesthetized mouse (80 mg/kg intra-peritoneal) was used. Damage to the muscle from eccentric contr...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1013188001776
更新日期:2001-01-01 00:00:00
abstract::In mammalian ventricles, two myosin heavy chain (MHC) isoforms have been identified. Small animals express α-MHC, whereas large animals express β-MHC, which contribute to a large difference in the heart rate. Sprague-Dawley rats possessing ~99% α-MHC were treated with propylthiouracil to result in 100% β-MHC. Papillar...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-016-9456-2
更新日期:2016-12-01 00:00:00
abstract::It is generally thought that the rapid relaxation of fast muscles is facilitated by the Ca(2+) binding protein parvalbumin (Parv). Indeed superfast swimbladder (SWB) muscle of toadfish contains the largest concentration of this protein ever observed (up to 1.5 mM). At 15 degrees C toadfish perform a 100 Hz call, 400 m...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-009-9175-z
更新日期:2009-01-01 00:00:00
abstract::Changes in the isoform patterns of myosin heavy chains (MHC) and Ca2+-ATPase (SERCA) were studied in long-term (72 days) denervated slow-twitch soleus muscles of euthyroid and hyperthyroid rabbits. MHC isoforms were separated electrophoretically. SERCA isoforms were assessed by electrophoretic separation of the trypsi...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1014543507149
更新日期:2001-01-01 00:00:00
abstract::In this study the spontaneous activities of prescapular lymph nodes, which were isolated from calves, goats and sheep and mesenteric lymph nodes of guinea-pigs were recorded and analysed in the frequency domain. Stretch-evoked contractions of the mesenteric lymph nodes were also recorded and their frequency characteri...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00711961
更新日期:1983-02-01 00:00:00