Molecular characterization of myosin V from Drosophila melanogaster.

Abstract:

:Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement. In this paper we report the molecular characterization of the Drosophila myosin V, identified by reverse genetics. The gene encodes a 1792-residue, 207 kDa heavy chain polypeptide which possesses a typical head or motor domain of 771 residues, a region of six IQ motifs (139 residues) which serve as potential calmodulin/light chain binding sites at the head/tail junction, and a tail domain of 882 residues containing sequences of putative alpha-helical coiled-coils required for dimerization of the molecule and sequences of non-helical structure at the C-terminal end. Based on Southern blot analyses and chromosomal localization, evidence is presented for a single Drosophila myosin V gene. RNA analyses revealed a doublet of transcripts of about 6 kb, expressed throughout the lifetime of a fly but particularly abundant in the early stages of embryonic development (maternally contributed), in the ectodermic tissue of the hindgut starting at stage 16, and in the adult head. These results suggest that myosin V may be involved in processes required in a variety of cell types in Drosophila. We have also mapped the Drosophila myosin V locus to chromosome 2 at the position 43C-D, and we are currently searching for known mutations in this region. Finally, phylogenetic analysis of the head domain reveals that Drosophila myosin V is more closely related to mammalian myosin Va and Vb than to other invertebrate class-V myosins; nevertheless, it is not significantly more related to myosin Va than to myosin Vb. While vertebrates would need two different myosin V isoforms to accomplish specific functions, we speculate that Drosophila myosin V might provide the equivalent functions by itself.

authors

Bonafé N,Sellers JR

doi

10.1023/a:1005356511634

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

129-41

issue

2

eissn

0142-4319

issn

1573-2657

journal_volume

19

pub_type

杂志文章
  • Rapid decrease in active tension generated by C2C12 myotubes after termination of artificial exercise.

    abstract::We found that the active tension of C2C12 myotubes that had been subjected to artificial exercise for ~10 days decreased rapidly after termination of the artificial exercise. When differentiated C2C12 myotubes were subjected to continuous 1 Hz artificial exercise for ~10 days, the active tension increased to ~4× compa...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-010-9230-9

    authors: Fujita H,Hirano M,Shimizu K,Nagamori E

    更新日期:2010-12-01 00:00:00

  • Some binding properties of Omp T digested muscle tropomyosin.

    abstract::Cleavage of vertebrate muscle tropomyosin by bacterial Omp T produces an amino-terminally truncated product (residues 7-284). The proteolysed protein, which is resolved from the parent by electrophoresis in the presence of sodium dodecylsulphate, can be generated from a variety of striated and smooth muscle tropomyosi...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-007-9114-9

    authors: Goonasekara CL,Gallivan LJ,Jackman DM,Heeley DH

    更新日期:2007-01-01 00:00:00

  • Cold shock protein RBM3 attenuates atrophy and induces hypertrophy in skeletal muscle.

    abstract::RNA-binding motif protein 3 (RBM3), a stress-inducible RNA-binding protein that increases protein synthesis and confers cell protection in multiple cell types, has been identified as a possible regulator of skeletal muscle mass. Therefore, the primary aim of this study was to examine the impact of elevated RBM3 on ske...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-018-9496-x

    authors: Van Pelt DW,Confides AL,Judge AR,Vanderklish PW,Dupont-Versteegden EE

    更新日期:2018-04-01 00:00:00

  • Inactivation of Ca2+ transients in amphibian and mammalian muscle fibres.

    abstract::MagFluo-4 fluorescence (Ca2+) transients associated with action potentials were measured in intact muscle fibres, manually dissected from toads ( Leptodactylus insularis ) or enzymatically dissociated from mice. In toads, the decay phase of the Ca2+ transients is described by a single exponential with a time constant ...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-004-4071-z

    authors: Caputo C,Bolaños P,Gonzalez A

    更新日期:2004-01-01 00:00:00

  • Assembly of the cardiac I-band region of titin/connectin: expression of the cardiac-specific regions and their structural relation to the elastic segments.

    abstract::The giant molecule titin (also called connectin) provides an elastic connection in the I-band between the Z-disk and A-band of striated muscle. This region is assembled in a tissue-specific way by extensive differential splicing events. We have raised monoclonal antibodies against the two N2-line isoforms of titin and...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF00123361

    authors: Gautel M,Lehtonen E,Pietruschka F

    更新日期:1996-08-01 00:00:00

  • Intermediate filament proteins increase during chronic stimulation of skeletal muscle.

    abstract::Chronic low-frequency electrical stimulation of rabbit fast-twitch skeletal muscle induces increased levels of two intermediate filament proteins, desmin and vimentin, during the first 3 weeks of stimulation. These increases occur over the same timecourse as reported shifts in alpha-actinin expression and increased Z-...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF00130240

    authors: Baldi JC,Reiser PJ

    更新日期:1995-12-01 00:00:00

  • In vitro myoblast motility models: investigating migration dynamics for the study of skeletal muscle repair.

    abstract::Skeletal muscle repair requires the migration of myoblasts (activated satellite cells) both to the injury site and then within the wound to facilitate cellular alignment in preparation for differentiation, fusion and eventual healing. Along this journey, the cells encounter a range of soluble and extracellular matrix ...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章,评审

    doi:10.1007/s10974-013-9364-7

    authors: Goetsch KP,Myburgh KH,Niesler CU

    更新日期:2013-12-01 00:00:00

  • Insertion of alpha1S II-III loop and C terminal sequences into alpha1H fails to restore excitation-contraction coupling in dysgenic myotubes.

    abstract::The L-type Ca2+ channel in skeletal muscle (alpha1S) is essential for excitation-contraction (EC) coupling. Previous studies using chimeras composed of alpha1S together with alpha1C or alpha1M demonstrated the importance of the alpha1S II-III loop and of a smaller subdomain (residues 720-764; 'ECC') in skeletal EC cou...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1024830132118

    authors: Wilkens CM,Beam KG

    更新日期:2003-01-01 00:00:00

  • Nuclear localization of Myomesin-1: possible functions.

    abstract::Myomesin-I (also known as Skelemin) is a approximately 185 kDa protein, which is highly expressed in striated muscle. It contains the prototypic class-I (type-III fibronectin) and class-II (C2-immunoglobulin) motifs. Previous studies have shown the presence of Myomesin-I at the M-line of the sarcomere, where it is tho...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-008-9137-x

    authors: Reddy KB,Fox JE,Price MG,Kulkarni S,Gupta S,Das B,Smith DM

    更新日期:2008-01-01 00:00:00

  • Parvalbumins and muscle relaxation: a computer simulation study.

    abstract::The distribution of Ca2+ and Mg2+ among the 'regulatory' cation binding sites of troponin (T-sites) and the strong, Ca2+-Mg2+ binding sites of troponin and parvalbumins (P-sites) in the sarcoplasm of a muscle was calculated. At rest, 60% of the T-sites were metal free, while 92% of the P-sites were loaded with Mg2+. I...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF00712090

    authors: Gillis JM,Thomason D,Lefèvre J,Kretsinger RH

    更新日期:1982-12-01 00:00:00

  • Structure of filamin and the F-actin-heavy merofilamin complex.

    abstract::Rotary-shadowed filamin molecules appear as long, highly flexible rods curved into a variety of configurations. The particles observed were 2.7 nm wide but had contour lengths of either 98 nm or 193 nm. The longer particles are probably end-to-end dimers of the shorter but it is not clear how many polypeptide chains t...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF00711869

    authors: Castellani L,Offer G,Elliott A,O'Brien EJ

    更新日期:1981-06-01 00:00:00

  • Myosin isozymes in avian skeletal muscles. I. Sequential expression of myosin isozymes in developing chicken pectoralis muscles.

    abstract::Myosin has been purified from chicken pectoralis muscle at various stages of development, from 10 days' incubation to approximately 10 months after hatching. Embryonic myosin from the earliest stage showed a high level of ATPase activity, similar to that obtained for adult pectoralis myosin. Two-dimensional peptide ma...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF00712161

    authors: Lowey S,Benfield PA,LeBlanc DD,Waller GS

    更新日期:1983-12-01 00:00:00

  • Therapeutic clenbuterol treatment does not alter Ca2+ sensitivity of permeabilized fast muscle fibres from exercise trained or untrained horses.

    abstract::Clenbuterol is a beta2-adrenoceptor agonist primarily used for treating bronchospasm and alleviating the symptoms of chronic obstructive pulmonary disease (COPD) in the horse. In other species (rats, mice, sheep, and cattle), chronic high doses of clenbuterol (typically in the milligram per kilogram body weight range)...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1027377731137

    authors: Plant DR,Kearns CF,McKeever KH,Lynch GS

    更新日期:2003-01-01 00:00:00

  • The apparent rate constant for the dissociation of force generating myosin crossbridges from actin decreases during Ca2+ activation of skinned muscle fibres.

    abstract::The effect of Ca2+ activation on the apparent rate constant governing the dissociation of force generating myosin crossbridges was studied in skinned rabbit adductor magnus fibres (fast-twitch) at 21 +/- 1 degree C. Simultaneous measurements of Ca2(+)-activated isometric force and ATPase activity were conducted in par...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF01781174

    authors: Kerrick WG,Potter JD,Hoar PE

    更新日期:1991-02-01 00:00:00

  • Fibre types in Limulus telson muscles: morphology and histochemistry.

    abstract::Using a variety of techniques, we have demonstrated the presence of at least two fibre types in Limulus median telson levator muscle. By light and electron microscopy, large (2,156 microns 2 mean cross-sectional area) fibres have A-bands of 4.1 microns, one-half I bands of 2.15 microns and Z lines less than or equal t...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF01739856

    authors: Levine RJ,Davidheiser S,Kelly AM,Kensler RW,Leferovich J,Davies RE

    更新日期:1989-02-01 00:00:00

  • Swimming exercise in infancy has beneficial effect on the hearts in cardiomyopathic Syrian hamsters.

    abstract::The phenotypic expression of cardiomyopathy is greatly influenced by extrinsic factors other than intrinsic genetic defects, such as environmental stress. Exercise is assumed to be an important extrinsic factor, since sudden death is sometimes seen during exercise in young patients with hypertrophic cardiomyopathy (HC...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/b:jure.0000021353.82449.9e

    authors: Tatsuguchi M,Hiratsuka E,Machida S,Nishikawa T,Imamura S,Shimizu S,Nishimura M,Komuro I,Furutani Y,Furutani M,Nagao H,Komatsu K,Kasanuki H,Matsuoka R

    更新日期:2004-01-01 00:00:00

  • Selection for rapid growth increases the number and the size of muscle fibres without changing their typing in chickens.

    abstract::Quantitative (muscle fibre number and cross-sectional areas) and qualitative (myosin isoforms and metabolic enzyme activities) characteristics of two muscles, M. pectoralis major and M. anterior latissimus dorsi, were compared among male chickens of two lines during growth from hatching to adulthood. The lines were de...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF00122527

    authors: Remignon H,Gardahaut MF,Marche G,Ricard FH

    更新日期:1995-04-01 00:00:00

  • Comparison of sarcoplasmic reticulum capabilities in toadfish (Opsanus tau) sonic muscle and rat fast twitch muscle.

    abstract::The sonic muscle of the oyster toadfish, Opsanus tau, can produce unfused contractions at 300 Hz. Electron microscopy shows a great abundance of the Sarcoplasmic reticulum (SR) in this muscle, but no functional characterization of the capabilities of the SR has been reported. We measured the oxalate-supported Ca2+ upt...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1005333215172

    authors: Feher JJ,Waybright TD,Fine ML

    更新日期:1998-08-01 00:00:00

  • Stretchin-klp, a novel Drosophila indirect flight muscle protein, has both myosin dependent and independent isoforms.

    abstract::Stretchin-klp is a newly described protein in Drosophila indirect flight muscles (IFM) that migrates on SDS gels as two distinct components of approximately 225 and 231 kD. Although the larger isoform is IFM specific, the smaller stretchin-klp isoform is expressed not only in IFM, but also in wild-type tissues of the ...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-005-9012-y

    authors: Patel SR,Saide JD

    更新日期:2005-01-01 00:00:00

  • The effects of ramp stretches on active contractions in intact mammalian fast and slow muscle fibres.

    abstract::The effects of a ramp stretch (amplitude <6% muscle fibre length (L0), speed < 13L0 s(-1)) on twitch tension and twitch tension re-development were examined in intact mammalian (rat) fast and slow muscle fibre bundles. The experiments were done in vitro at 20 degrees C and at an initial sarcomere length of 2.68 microm...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1010556623905

    authors: Mutungi G,Ranatunga KW

    更新日期:2001-01-01 00:00:00

  • Shortening properties of two biochemically defined muscle fibre types of the Norway lobster Nephrops norvegicus L.

    abstract::Mechanical properties of myofibrillar bundles from single chemically skinned fibres from the superficial abdominal flexor muscle of the Norway lobster Nephrops norvegicus were measured, and the protein content of these fibres was analysed by SDS-PAGE. Two slow fibre phenotypes (S1, S2) were distinguished on the basis ...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1005481725344

    authors: Holmes JM,Hilber K,Galler S,Neil DM

    更新日期:1999-04-01 00:00:00

  • Gelatinase-B (matrix metalloproteinase-9; MMP-9) secretion is involved in the migratory phase of human and murine muscle cell cultures.

    abstract::The remodelling of connective tissue components is a fundamental requirement for a number of pivotal processes in cell biology. These may include myoblast migration and fusion during development and regeneration. In other systems, similar biological processes are facilitated by secretion of the matrix metalloproteinas...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1005670507906

    authors: Lewis MP,Tippett HL,Sinanan AC,Morgan MJ,Hunt NP

    更新日期:2000-04-01 00:00:00

  • Activation of skinned muscle fibres from the Norway lobster Nephrops norvegicus L. by manganese ions.

    abstract::Effects of Mn2+ and Ca2+ on the mechanical properties of glycerinated myofibrillar bundles originating from slow S1 type muscle fibres of superficial flexor muscles of the lobster Nephrops norvegicus were investigated. Mn2+ (5-20 microM) activated the preparations in a dose-dependent manner. The sensitivity of myofibr...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1005312610629

    authors: Holmes JM,Hilber K,Galler S,Neil DM

    更新日期:1998-06-01 00:00:00

  • Angle of active site of myosin heads in contracting muscle during sudden length changes.

    abstract::The change in orientation of myosin crossbridges in contracting muscle during sudden length changes was examined by fluorescence polarization. This study used a fluorescent ATP analogue, 1,N6-etheno-2-aza-ATP(epsilon-2-aza-ATP) as a probe. Its fluorescence is considerably enhanced upon binding with myosin and is depen...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF00712310

    authors: Yanagida T

    更新日期:1985-02-01 00:00:00

  • What do we know about what happens to myometrial function as women age?

    abstract::Much has been written about the effects of aging on reproductive function, especially female fertility. Much less is known about how aging may affect the contractility of the smooth muscle within the uterus, the myometrium. The myometrium is active through a woman's entire life, not just during pregnancy. Here we will...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-012-9300-2

    authors: Arrowsmith S,Robinson H,Noble K,Wray S

    更新日期:2012-08-01 00:00:00

  • Electrostatic interactions in the SH1-SH2 helix of human cardiac myosin modulate the time of strong actomyosin binding.

    abstract::Two single mutations, R694N and E45Q, were introduced in the beta isoform of human cardiac myosin to remove permanent salt bridges E45:R694 and E98:R694 in the SH1-SH2 helix of the myosin head. Beta isoform-specific bridges E45:R694 and E98:R694 were discovered in the molecular dynamics simulations of the alpha and be...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/s10974-020-09588-1

    authors: Gargey A,Iragavarapu SB,Grdzelishvili AV,Nesmelov YE

    更新日期:2020-09-14 00:00:00

  • M-band: a safeguard for sarcomere stability?

    abstract::The sarcomere of striated muscle is a very efficient machine transforming chemical energy into movement. However, a wrong distribution of the generated forces may lead to self-destruction of the engine itself. A well-known example for this is eccentric contraction (elongation of the sarcomere in the activated state), ...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章,评审

    doi:10.1023/a:1026094924677

    authors: Agarkova I,Ehler E,Lange S,Schoenauer R,Perriard JC

    更新日期:2003-01-01 00:00:00

  • Expression of a novel combination of fast and slow troponin T isoforms in rabbit extraocular muscles.

    abstract::The properties of extraocular muscles (EOMs) are quite different from those of the trunk and limb. Here we show that there is a novel pattern of troponin T (TnT) expression in EOMs which most likely contributes to the fine control of ocular movement and may reflect their innervation by cranial motoneurons. Three regio...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1007/BF01773894

    authors: Briggs MM,Jacoby J,Davidowitz J,Schachat FH

    更新日期:1988-06-01 00:00:00

  • Comparison of cross-bridge cycling kinetics in neonatal vs. adult rat ventricular muscle.

    abstract::The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was d...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1005585807179

    authors: Prakash YS,Cody MJ,Housmans PR,Hannon JD,Sieck GC

    更新日期:1999-10-01 00:00:00

  • Effects of long-term phasic electrical stimulation on denervated soleus muscle: guinea-pig contrasted with rat.

    abstract::Guinea-pig soleus muscles were denervated and electrically stimulated for periods of 43 to 66 days. Stimuli were in 1 s bursts of 40 Hz pulses, repeated every 5 min. Other guinea-pigs were denervated for 82 days without stimulation and, in a third group, the soleus muscle was necrotized and allowed to regenerate witho...

    journal_title:Journal of muscle research and cell motility

    pub_type: 杂志文章

    doi:10.1023/a:1018619503456

    authors: Lewis DM,al-Amood WS,Schmalbruch H

    更新日期:1997-10-01 00:00:00