Abstract：
:This study investigated the effects of the protein kinase A (PKA) inhibitor, H-89, in mechanically-skinned muscle fibres and intact muscle fibres, in order to determine whether PKA phosphorylation is essential for normal excitation-contraction (E-C) coupling. In skinned EDL fibres of the rat, force responses to depolarization (by ion substitution) were inhibited only slightly by 10 microM H-89, a concentration more than sufficient to fully inhibit PKA. Staurosporine (1 microM), a potent non-specific kinase inhibitor, also had little if any effect on depolarization-induced responses. At 1-2 microM, H-89 significantly slowed the repriming rate in rat skinned fibres, most likely due to it deleteriously affecting the T-system potential. With 100 microM H-89, the force response to depolarization by ion substitution was completely abolished. This inhibitory effect was reversed by washout of H-89 and was not due to block of the Ca2+ release channel in the sarcoplasmic reticulum (SR). In intact single fibres of the flexor digitorum longus (FDB) muscle of the mouse, 1-3 microM H-89 had no noticeable effect on action-potential-mediated Ca2+ transients. Higher concentrations (4-10 microM) caused Ca2+ transient failure in fibres stimulated at 20 Hz in a manner indicative of action-potential failure. At 10-100 microM, H-89 also inhibited net Ca2+ uptake by the SR and affected the Ca2+-sensitivity of the contractile apparatus in rat skinned fibres. All such effects were proportionately greater in toad muscle fibres. These results do not support the hypothesis that phosphorylation is essential for the Ca2+ release channel to open in response to voltage-sensor activation in skeletal muscle fibres.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Blazev R,Hussain M,Bakker AJ,Head SI,Lamb GDdoi
10.1023/a:1012289526618keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
277-86issue
3eissn
0142-4319issn
1573-2657journal_volume
22pub_type
杂志文章abstract：:Myosin has been purified from chicken pectoralis muscle at various stages of development, from 10 days' incubation to approximately 10 months after hatching. Embryonic myosin from the earliest stage showed a high level of ATPase activity, similar to that obtained for adult pectoralis myosin. Two-dimensional peptide ma...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712161
更新日期：1983-12-01 00:00:00
abstract：:Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01774035
更新日期：1991-04-01 00:00:00
abstract：:Creatine kinase (CK) isoenzyme compositions of different types of single muscle fibres dissected from soleus (SOL) and extensor digitorum longus (EDL) muscles from rats were examined. CK isoenzymes were separated into cytoplasmic (CK-MM, CK-MB, CK-BB) (muscle, brain and hybrid types, respectively) and mitochondrial (m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01781172
更新日期：1991-02-01 00:00:00
abstract：:Five monoclonal antibodies that react with different regions of myosin light chain 1 from human ventricular myocardial muscle were used to obtain information on interactions between the light chain 1 and heavy chains and generally on the tertiary structure of the light chain 1 within the myosin head. We performed West...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01766461
更新日期：1992-06-01 00:00:00
abstract：:temporaria (0.9-2.5degrees C) were stimulated to produce a 1 s isometric tetanus at regular intervals until constant mechanical responses were attained. Various degrees of force depression ("fatigue") were produced by decreasing the contraction interval from 30 or 15 min (control to 120, 60, 30 and 15s, respectively. ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00713270
更新日期：1981-09-01 00:00:00
abstract：:The fine structure of leech body wall muscles, has been analysed under great length variations. All the measurable parameters of the fibre (distance between thick filaments, sarcomere and A band width, percentage of actin crowns around myosin filaments, fibre radius) at different elongations were tested with equations...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711915
更新日期：1985-10-01 00:00:00
abstract：:MagFluo-4 fluorescence (Ca2+) transients associated with action potentials were measured in intact muscle fibres, manually dissected from toads ( Leptodactylus insularis ) or enzymatically dissociated from mice. In toads, the decay phase of the Ca2+ transients is described by a single exponential with a time constant ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-004-4071-z
更新日期：2004-01-01 00:00:00
abstract：:The distribution of Ca2+ and Mg2+ among the 'regulatory' cation binding sites of troponin (T-sites) and the strong, Ca2+-Mg2+ binding sites of troponin and parvalbumins (P-sites) in the sarcoplasm of a muscle was calculated. At rest, 60% of the T-sites were metal free, while 92% of the P-sites were loaded with Mg2+. I...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712090
更新日期：1982-12-01 00:00:00
abstract：:Trabeculae from the right ventricle of rat were chemically 'skinned' with saponin (50 micrograms ml-1 for 30 min). Caffeine (10 mM) induced a transient contracture as a consequence of Ca(2+)-release from the sarcoplasmic reticulum and subsequent activation of the myofilaments. The amplitude of the caffeine contracture...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01874151
更新日期：1992-04-01 00:00:00
abstract：:Melatonin (N-acetyl-5-methoxy-tryptamine) is an effective antioxidant and free radical scavenger, that has important biological effects in multiple cell types and species. Melatonin research in muscle has recently gained attention, mainly focused on its role in cells or tissue repair and regeneration after injury, due...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-020-09578-3
更新日期：2020-09-01 00:00:00
abstract：:When skinned skeletal muscle fibres with rest sarcomere length (L = 2.5 microns) are compressed by the addition of various concentrations ([PVP]) of polyvinylpyrrolidine, the relation between the 1,0 spacing (d) of thick filament lattice and [PVP] has been known to break at d of around 35 nm, resulting in a steeper sl...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738331
更新日期：1991-10-01 00:00:00
abstract：:Small but statistically significant shifts (hysteresis) between the up (increasing tension direction) and down (decreasing tension) pCa/tension relation for several muscle types of the rabbit and rat were found. The largest shift is, at most, 0.093 p units or 60% of that reported for the barnacle muscle. The direction...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00713061
更新日期：1985-04-01 00:00:00
abstract：:The mRNA levels of the adult and the neonatal sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPases (SERCA1a and SERCA1b, respectively) and those of the muscle regulatory factors (MRFs: myoD, myf-5, myogenin, MRF4) have been assessed by RT PCR in rat soleus muscles immobilized for 3 days in an extended position (passive s...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005541522599
更新日期：1999-05-01 00:00:00
abstract：:White muscle fibres from dogfish were used to compare the energetic costs of shortening by fully active muscle and by relaxing muscle. The muscle preparation was tetanized for 0.6 s and shortened either during stimulation or during relaxation. The distance shortened was 1 mm (about 15% L0, the muscle length optimum fo...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005455505056
更新日期：1998-10-01 00:00:00
abstract：:2,3-butanedione 2-monoxime (BDM, 3-30 mM) decreased twitch force of intact ventricular trabeculae isolated from 19-day embryonic chick hearts in a dose-dependent manner. The responses to BDM were rapid and reversible. In an attempt to determine the cellular basis for the inhibitory effect of BDM, experiments were carr...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00125305
更新日期：1995-02-01 00:00:00
abstract：:The ATP-ADP exchange reaction and its related partial reactions of fragmented sarcoplasmic reticulum from bullfrog skeletal muscle (frog FSR) were investigated and compared with those of rabbit FSR in order to understand the characteristics of calcium-activated ATPase (Ca2+-ATPase) of frog FSR. MgATP and magnesium-fre...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711879
更新日期：1982-03-01 00:00:00
abstract：:The change in orientation of myosin crossbridges in contracting muscle during sudden length changes was examined by fluorescence polarization. This study used a fluorescent ATP analogue, 1,N6-etheno-2-aza-ATP(epsilon-2-aza-ATP) as a probe. Its fluorescence is considerably enhanced upon binding with myosin and is depen...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712310
更新日期：1985-02-01 00:00:00
abstract：:The pattern of expression of different types of myosin heavy chains and the development of different generations of muscle cells during foetal life were studied in four bovine muscles with widely varying characteristics, the Masseter, Longissimus thoracis, Cutaneus trunci and Diaphragma. Different complementary techni...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00122120
更新日期：1994-08-01 00:00:00
abstract：:Dr. John Gergely passed away on July 26, 2013 after a long and distinguished career. His publications spanned 67 years. He founded the Department of Muscle Research in the Retina Foundation (which later became the Boston Biomedical Research Institute) and served as director for 34 years. Dr. Gergely served on the edit...
journal_title：Journal of muscle research and cell motility
pub_type： 传,历史文章,杂志文章
doi：10.1007/s10974-013-9370-9
更新日期：2013-12-01 00:00:00
abstract：:Nebulin is an integral protein of skeletal muscle thin filaments and probably acts as a ruler for the thin filament length. Cardiac muscles of higher vertebrates have been shown earlier to lack nebulin. Instead in human and chicken cardiac muscle the much smaller protein nebulette replaces nebulin. Since nebulette is ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1020909902462
更新日期：2002-01-01 00:00:00
abstract：:Two of the most characteristic features of striated muscle are (i) its ability to contract and generate tension when activated and (ii) its ability to return to its original length and form after contraction or stretching ceases. These two properties are to a large extent the primary manifestations of separate sets of...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/BF00123477
更新日期：1994-06-01 00:00:00
abstract：:The aim of the present study was to examine the temperature dependence of active force in lengthening and shortening muscle. Experiments were done, in vitro, on bundles of intact fibres (fibre length L(0) ~2 mm; sarcomere length ~2.5 μm) isolated from a rat fast muscle (flexor hallucis brevis) and a ramp length change...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-012-9307-8
更新日期：2012-10-01 00:00:00
abstract：:Using a variety of techniques, we have demonstrated the presence of at least two fibre types in Limulus median telson levator muscle. By light and electron microscopy, large (2,156 microns 2 mean cross-sectional area) fibres have A-bands of 4.1 microns, one-half I bands of 2.15 microns and Z lines less than or equal t...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01739856
更新日期：1989-02-01 00:00:00
abstract：:Cells isolated from pig urinary bladders and pregnant full term human uteruses were attached longitudinally between a microforce transducer and a length displacement apparatus. Cells were stretched by applying a series of ramp-like length changes of 0.2 s duration and 10.0 microns amplitude at intervals of 15 min. Pas...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01739765
更新日期：1990-10-01 00:00:00
abstract：:Isolated frog muscle fibres of four different lengths (L0, initial; L1, 15%; L2, 25%; and L3, 35% stretched vs. L0) were stimulated continuously (3 min) by suprathreshold rectangular pulses at three different frequencies (f1 = 5 Hz, f2 = 6.7 Hz and f3 = 10 Hz). Using a pair of electrodes mounted at a fixed distance, t...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The force-velocity (T-V) relation and the force-extension (T1) relation from single fibres isolated from the muscle tibialis anterior of the frog were determined at present times during the rise of tension and the plateau of an isometric tetanus. During the rise of an isometric tetanus the value of V0 (the velocity of...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00712029
更新日期：1983-04-01 00:00:00
abstract：:The myosin II motor from Dictyostelium discoideum has been engineered to contain single tryptophan residues at strategic locations to probe movements of switch 1 and switch 2. The tryptophan residue at W501 probes movement of the relay helix and indirectly reports on switch 2 movement. This probe suggests that there i...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-005-9004-y
更新日期：2005-01-01 00:00:00
abstract：:Cytokinesis is the mechanical process that allows the simplest unit of life, the cell, to divide, propagating itself. To divide, the cell converts chemical energy into mechanical energy to produce force. This process is thought to be active, due in large part to the mechanochemistry of the myosin-II ATPase. The cell's...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1023/a:1024419510314
更新日期：2002-01-01 00:00:00
abstract：:Long-(SL > 6 microns) and short-sarcomere (SL < 4 microns) fibres were isolated from the claw muscle of the yabby (Cherax destructor) during limb regeneration and at different stages of the moult cycle. Long-sarcomere fibres were more susceptible to the changes resulting from the moult-induced atrophy compared with th...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00121136
更新日期：1995-06-01 00:00:00
abstract：:Skeletal muscle tissue is mechanosensitive, as it is able to sense mechanical impacts and to translate these into biochemical signals making the tissue adapt. Among its mechanosensitive nature, skeletal muscle tissue is the largest metabolic organ of the human body. Disturbances in skeletal muscle mechanosensing and m...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-017-9489-1
更新日期：2017-08-01 00:00:00
