Abstract:
:A balanced redox status is necessary to optimize force production in contractile apparatus, where free radicals generated by skeletal muscle are involved in some basic physiological processes like excitation-contraction coupling. Protein glutathionylation has a key role in redox regulation of proteins and signal transduction. Here we show that myosin is sensitive to in vitro glutathionylation and MALDI-TOF analysis identified three potential sites of glutathione binding, two of them locating on the myosin head. Glutathionylation of myosin has an important impact on the protein structure, as documented by the lower fluorescence quantum yield of glutathionylated myosin and its increased susceptibility to the proteolytic cleavage. Myosin function is also sensitive to glutathionylation, which modulates its ATPase activity depending on GSSG redox balance. Thus, like the phosphorylation/dephosphorylation cycle, glutathionylation may represent a mechanism by which glutathione modulates sarcomere functions depending on the tissue redox state, and myosin may constitute a muscle redox-sensor.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Passarelli C,Petrini S,Pastore A,Bonetto V,Sale P,Gaeta LM,Tozzi G,Bertini E,Canepari M,Rossi R,Piemonte Fdoi
10.1007/s10974-008-9145-xsubject
Has Abstractpub_date
2008-01-01 00:00:00pages
119-26issue
2-5eissn
0142-4319issn
1573-2657journal_volume
29pub_type
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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