Myosin and SERCA isoform expression in denervated slow-twitch muscle of euthyroid and hyperthyroid rabbits.

abstract：:We investigated the regulatory roles of MgADP and free Ca2+ in isometric tension development in skinned bovine cardiac muscle. We found that, in the relaxed state without free Ca2+, MgADP elicited a sigmoidal increase in active tension, as is the case in skeletal muscle (ADP-contraction). The critical MgADP concentrat...

journal_title：Journal of muscle research and cell motility

authors： Fukuda N,Fujita H,Fujita T,Ishiwata S

更新日期：1998-11-01 00:00:00

abstract：:Chronic low-frequency electrical stimulation of rabbit fast-twitch skeletal muscle induces increased levels of two intermediate filament proteins, desmin and vimentin, during the first 3 weeks of stimulation. These increases occur over the same timecourse as reported shifts in alpha-actinin expression and increased Z-...

journal_title：Journal of muscle research and cell motility

authors： Baldi JC,Reiser PJ

更新日期：1995-12-01 00:00:00

abstract：:The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was d...

journal_title：Journal of muscle research and cell motility

authors： Prakash YS,Cody MJ,Housmans PR,Hannon JD,Sieck GC

更新日期：1999-10-01 00:00:00

abstract：:Myosin heavy chain kinase activity was identified in the high speed supernate of lysed Dictyostelium amoebae and was precipitated by 30-50% ammonium sulphate. In low ionic strength buffer, the activity bound tightly to a Cibacron Blue Sepharose column and eluted as a single peak with 1.0 M NaCl. Gel filtration chromat...

journal_title：Journal of muscle research and cell motility

authors： Kuczmarski ER

更新日期：1986-12-01 00:00:00

abstract：:Reactive oxygen species (ROS) are generated in skeletal muscle both during the rest and contractile activity. Myogenic cells are equipped with antioxidant enzymes, like superoxide dismutase, catalase, glutathione peroxidase, γ-glutamylcysteine synthetase and heme oxygenase-1. These enzymes not only neutralise excessiv...

journal_title：Journal of muscle research and cell motility

authors： Kozakowska M,Pietraszek-Gremplewicz K,Jozkowicz A,Dulak J

更新日期：2015-12-01 00:00:00

abstract：:Effects of Mn2+ and Ca2+ on the mechanical properties of glycerinated myofibrillar bundles originating from slow S1 type muscle fibres of superficial flexor muscles of the lobster Nephrops norvegicus were investigated. Mn2+ (5-20 microM) activated the preparations in a dose-dependent manner. The sensitivity of myofibr...

journal_title：Journal of muscle research and cell motility

authors： Holmes JM,Hilber K,Galler S,Neil DM

更新日期：1998-06-01 00:00:00

abstract：:A transducer has been developed for measuring the minute forces generated during isometric contractions (1.0-10.0 microN) of single smooth muscle cells from the pig urinary bladder and the human uterus. In addition to its high sensitivity, resolution and stability (100 mV microN-1, and less than 0.1 microN and less th...

journal_title：Journal of muscle research and cell motility

authors： Glerum JJ,Van Mastrigt R

更新日期：1990-08-01 00:00:00

abstract：:We investigated the expression of neuronal nitric oxide synthase (nNOS) and dystrophin in the regenerating skeletal muscles of rats after cardiotoxin-induced myonecrosis by immunohistochemical studies and western blot analysis. In normal muscles, nNOS was moderately immunostained on type 2B fibers, but was faintly imm...

journal_title：Journal of muscle research and cell motility

authors： Hoshino S,Ohkoshi N,Ishii A,Shoji S

更新日期：2002-01-01 00:00:00

abstract：:Trabeculae from the right ventricle of rat were chemically 'skinned' with saponin (50 micrograms ml-1 for 30 min). Caffeine (10 mM) induced a transient contracture as a consequence of Ca(2+)-release from the sarcoplasmic reticulum and subsequent activation of the myofilaments. The amplitude of the caffeine contracture...

journal_title：Journal of muscle research and cell motility

authors： Steele DS,Miller DJ

更新日期：1992-04-01 00:00:00

abstract：:Two single mutations, R694N and E45Q, were introduced in the beta isoform of human cardiac myosin to remove permanent salt bridges E45:R694 and E98:R694 in the SH1-SH2 helix of the myosin head. Beta isoform-specific bridges E45:R694 and E98:R694 were discovered in the molecular dynamics simulations of the alpha and be...

journal_title：Journal of muscle research and cell motility

authors： Gargey A,Iragavarapu SB,Grdzelishvili AV,Nesmelov YE

更新日期：2020-09-14 00:00:00

abstract：:In this study the spontaneous activities of prescapular lymph nodes, which were isolated from calves, goats and sheep and mesenteric lymph nodes of guinea-pigs were recorded and analysed in the frequency domain. Stretch-evoked contractions of the mesenteric lymph nodes were also recorded and their frequency characteri...

journal_title：Journal of muscle research and cell motility

authors： Tümer A,Oztürk-Demir N,Basar-Eroğlu C,Noyan A

更新日期：1983-02-01 00:00:00

abstract：:The remodelling of connective tissue components is a fundamental requirement for a number of pivotal processes in cell biology. These may include myoblast migration and fusion during development and regeneration. In other systems, similar biological processes are facilitated by secretion of the matrix metalloproteinas...

journal_title：Journal of muscle research and cell motility

authors： Lewis MP,Tippett HL,Sinanan AC,Morgan MJ,Hunt NP

更新日期：2000-04-01 00:00:00

abstract：:There is evidence to suggest that 8 nm calcium transport particles in the sarcoplasmic reticulum are involved in the regulation of twitch properties in adult muscles. We have studied ultrastructural characteristics of the sarcoplasmic reticulum in relation to previously defined physiological changes that take place in...

journal_title：Journal of muscle research and cell motility

authors： Gauthier GF,Hobbs AW

更新日期：1986-04-01 00:00:00

abstract：:Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...

journal_title：Journal of muscle research and cell motility

authors： Goldfine SM,Einheber S,Fischman DA

更新日期：1991-04-01 00:00:00

abstract：:We examined the tension responses induced by rapid temperature-jumps (T-jumps) applied at different times during twitch and tetanic contractions in small intact fibre bundles (5-10 fibres) isolated from a fast foot muscle (flexor hallucis brevis) of the rat. A rapid T-jump of 2-4 degrees C was induced by a 0.2 ms infr...

journal_title：Journal of muscle research and cell motility

authors： Coupland ME,Pinniger GJ,Ranatunga KW

更新日期：2005-01-01 00:00:00

abstract：:Calcium overload is a fundamental pathogenic event associated with chronic muscle degeneration in muscular dystrophies. The possibility that L-type voltage-dependent calcium channels were involved in the etiology of chicken muscular dystrophy was investigated by studying the dihydropyridine receptors in transverse tub...

journal_title：Journal of muscle research and cell motility

authors： Moro G,Saborido A,Delgado J,Molano F,Megias A

更新日期：1995-10-01 00:00:00

abstract：:Previous studies have shown that lengthening contractions, in contrast to isometric contractions, readily result in sustained malfunctioning of the exercised muscles. The present study was performed to investigate whether an exercise period with many (240) lengthening contractions (LC) results in alterations in muscle...

journal_title：Journal of muscle research and cell motility

authors： Hesselink MK,Kuipers H,Keizer HA,Drost MR,van der Vusse GJ

更新日期：1998-05-01 00:00:00

abstract：:The characteristics of the titin filament in the vicinity of the Z-line were investigated using immunoelectron microscopy. We used monoclonal titin antibodies T-11 and T-12 on single fibres of frog skeletal muscle, and on Z-line-extracted fibres. It is well established that the I-band region of titin is elastic. We fi...

journal_title：Journal of muscle research and cell motility

authors： Trombitás K,Pollack GH

更新日期：1993-08-01 00:00:00

abstract：:Calsequestrin type-1 (CASQ1), the main sarcoplasmic reticulum (SR) Ca(2+) binding protein, plays a dual role in skeletal fibers: a) it provides a large pool of rapidly-releasable Ca(2+) during excitation-contraction (EC) coupling; and b) it modulates the activity of ryanodine receptors (RYRs), the SR Ca(2+) release ch...

journal_title：Journal of muscle research and cell motility

authors： Protasi F,Paolini C,Canato M,Reggiani C,Quarta M

更新日期：2011-12-01 00:00:00

abstract：:Long-(SL > 6 microns) and short-sarcomere (SL < 4 microns) fibres were isolated from the claw muscle of the yabby (Cherax destructor) during limb regeneration and at different stages of the moult cycle. Long-sarcomere fibres were more susceptible to the changes resulting from the moult-induced atrophy compared with th...

journal_title：Journal of muscle research and cell motility

authors： West JM,Humphris DC,Stephenson DG

更新日期：1995-06-01 00:00:00

abstract：:The sarcomere of striated muscle is a very efficient machine transforming chemical energy into movement. However, a wrong distribution of the generated forces may lead to self-destruction of the engine itself. A well-known example for this is eccentric contraction (elongation of the sarcomere in the activated state), ...

journal_title：Journal of muscle research and cell motility

authors： Agarkova I,Ehler E,Lange S,Schoenauer R,Perriard JC

更新日期：2003-01-01 00:00:00

abstract：:The in vivo state of assembly of myosin in vertebrate smooth muscle is controversial. In vitro studies on purified smooth muscle myosin show that it is monomeric (10S) under relaxing conditions and filamentous under contraction conditions. Electron microscopic and antibody labelling studies of intact smooth muscles, o...

journal_title：Journal of muscle research and cell motility

authors： Xu JQ,Gillis JM,Craig R

更新日期：1997-06-01 00:00:00

abstract：:In Drosophila melanogaster two high molecular weight tropomyosin isoforms, historically named heavy troponins (TnH-33 and TnH-34), are encoded by the Tm1 tropomyosin gene. They are specifically expressed in the indirect flight muscles (IFM). Their N-termini are conventional and complete tropomyosin sequences, but thei...

journal_title：Journal of muscle research and cell motility

authors： Mateos J,Herranz R,Domingo A,Sparrow J,Marco R

更新日期：2006-01-01 00:00:00

abstract：:Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement. In this paper we report the molecular characterization of the Drosophila myosin V, identified by reverse genetics. The gene encodes a 1792-residue, 207 kDa heavy chain polypeptide which p...

journal_title：Journal of muscle research and cell motility

authors： Bonafé N,Sellers JR

更新日期：1998-02-01 00:00:00

abstract：:The five possible analogues of ATP and the three possible analogues of ADP which contain single non-bridging sulphur atoms instead of oxygen in the polyphosphate structure have been used as probes of the interaction of nucleotides with myosin and actomyosin. Evidence is presented for the requirement of an alpha, beta,...

journal_title：Journal of muscle research and cell motility

authors： Goody RS,Hofmann W

更新日期：1980-03-01 00:00:00

abstract：:To explain observed differences in the activation dependence of force redevelopment kinetics between cardiac and skeletal muscle, two numerical models of contractile regulation by Ca2+ were investigated. Ca2+ binding and force production were each modelled as two-state processes with forward and reverse rate constants...

journal_title：Journal of muscle research and cell motility

authors： Hancock WO,Huntsman LL,Gordon AM

更新日期：1997-12-01 00:00:00

abstract：:The effect of urocortin (Uro), a recently discovered neuropeptide with selectivity towards corticotropin-releasing hormone type 2 receptor, was tested on whole cell currents expressed by guinea-pig gastric antrum smooth muscle cells. Uro (1 pmol/l-1 nmol/l) caused a concentration-dependent increase of Ca2+-sensitive K...

journal_title：Journal of muscle research and cell motility

authors： Petkova-Kirova PS,Gagov HS,Duridanova DB

更新日期：2000-01-01 00:00:00

abstract：:The distribution of Ca2+ and Mg2+ among the 'regulatory' cation binding sites of troponin (T-sites) and the strong, Ca2+-Mg2+ binding sites of troponin and parvalbumins (P-sites) in the sarcoplasm of a muscle was calculated. At rest, 60% of the T-sites were metal free, while 92% of the P-sites were loaded with Mg2+. I...

journal_title：Journal of muscle research and cell motility

authors： Gillis JM,Thomason D,Lefèvre J,Kretsinger RH

更新日期：1982-12-01 00:00:00

abstract：:Because complex structural differences in adult extraocular muscles may have physiological and pathophysiological significance, the three-dimensional pattern of myosin heavy chain (MHC) isoform expression within the orbital and global layers of the muscle bellies compared with the distal tendon ends was quantitatively...

journal_title：Journal of muscle research and cell motility

authors： McLoon LK,Rios L,Wirtschafter JD

更新日期：1999-11-01 00:00:00

abstract：:The details of the global and local structure and function of titin, a giant filamentous intrasarcomeric protein are largely undiscovered. Here we discuss a combination of bulk-solution and novel single-molecule techniques that may lend unique insights into titin's molecular dynamic, structural and mechanical characte...

journal_title：Journal of muscle research and cell motility

authors： Kellermayer MS,Grama L

更新日期：2002-01-01 00:00:00