Abstract:
:In Drosophila melanogaster two high molecular weight tropomyosin isoforms, historically named heavy troponins (TnH-33 and TnH-34), are encoded by the Tm1 tropomyosin gene. They are specifically expressed in the indirect flight muscles (IFM). Their N-termini are conventional and complete tropomyosin sequences, but their C-termini consist of different IFM-specific domains that are rich in proline, alanine, glycine and glutamate. The evidence indicates that in Diptera these IFM-specific isoforms are conserved and are not troponins, but heavy tropomyosins (TmH). We report here that they are post-translationally modified by several phosphorylations in their C-termini in mature flies, but not in recently emerged flies that are incapable of flight. From stoichiometric measurements of thin filament proteins and interactions of the TmH isoforms with the standard Drosophila IFM tropomyosin isoform (protein 129), we propose that the TmH N-termini are integrated into the thin filament structural unit as tropomyosin dimers. The phosphorylated C-termini remain unlocated and may be important in IFM stretch-activation. Comparison of the Tm1 and Tm2 gene sequences shows a complete conservation of gene organisation in other Drosophilidae, such as Drosophila pseudoobscura, while in Anopheles gambiae only one exon encodes a single C-terminal domain, though overall gene organization is maintained. Interestingly, in Apis mellifera (hymenopteran), while most of the Tm1 and Tm2 gene features are conserved, the gene lacks any C-terminal exons. Instead these sequences are found at the 3' end of the troponin I gene. In this insect order, as in Lethocerus (hemipteran), the original designation of troponin H (TnH) should be retained. We discuss whether the insertion of the IFM-specific pro-ala-gly-glu-rich domain into the tropomyosin or troponin I genes in different insect orders may be related to proposals that the IFM stretch activation mechanism has evolved independently several times in higher insects.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Mateos J,Herranz R,Domingo A,Sparrow J,Marco Rdoi
10.1007/s10974-005-9044-3subject
Has Abstractpub_date
2006-01-01 00:00:00pages
189-201issue
3-4eissn
0142-4319issn
1573-2657journal_volume
27pub_type
杂志文章abstract::We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in both ATPase and motility assays. We demonstrate that skeletal muscle myosin (S...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-012-9316-7
更新日期:2012-12-01 00:00:00
abstract::The sarcomere length (SL) dependence of the calcium sensitivity of force was measured in skinned single twitch and slow-tonic muscle fibres from frog and toad. Twitch and slow-tonic fibres were characterized by location, appearance, physiological response to calcium and by protein band patterns from sodium-dodecyl-sul...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00132181
更新日期:1993-02-01 00:00:00
abstract::Rotary-shadowed filamin molecules appear as long, highly flexible rods curved into a variety of configurations. The particles observed were 2.7 nm wide but had contour lengths of either 98 nm or 193 nm. The longer particles are probably end-to-end dimers of the shorter but it is not clear how many polypeptide chains t...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00711869
更新日期:1981-06-01 00:00:00
abstract::Heart disease is a leading cause of death in patients with Duchenne muscular dystrophy (DMD). Patients with DMD lack the protein dystrophin, which is widely expressed in striated muscle. In skeletal muscle, the loss of dystrophin results in dramatically decreased expression of the dystrophin associated glycoprotein co...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-013-9362-9
更新日期:2013-12-01 00:00:00
abstract::The effect of urocortin (Uro), a recently discovered neuropeptide with selectivity towards corticotropin-releasing hormone type 2 receptor, was tested on whole cell currents expressed by guinea-pig gastric antrum smooth muscle cells. Uro (1 pmol/l-1 nmol/l) caused a concentration-dependent increase of Ca2+-sensitive K...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005653218639
更新日期:2000-01-01 00:00:00
abstract::Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01774035
更新日期:1991-04-01 00:00:00
abstract::The phenotypic expression of cardiomyopathy is greatly influenced by extrinsic factors other than intrinsic genetic defects, such as environmental stress. Exercise is assumed to be an important extrinsic factor, since sudden death is sometimes seen during exercise in young patients with hypertrophic cardiomyopathy (HC...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/b:jure.0000021353.82449.9e
更新日期:2004-01-01 00:00:00
abstract::Troponin T (TnT) is one of the muscle regulatory proteins and is thought to be related to unique contractile properties in diverse muscles and also to myogenesis. The expression of TnT isoforms in the chicken wing muscles was investigated by two-dimensional gel electrophoresis and immunoblotting with an antiserum agai...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1016369300532
更新日期:2001-01-01 00:00:00
abstract::In frog semitendinosus muscle the descending limb of the length-tension curve is shifted rightward relative to that of tibialis anterior. Both the plateau right corner and the zero-force intercept are equally shifted. To investigate the reason for this shift, we compared filament lengths in the two muscles. Single fib...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00115451
更新日期:1993-04-01 00:00:00
abstract::We examined the tension responses induced by rapid temperature-jumps (T-jumps) applied at different times during twitch and tetanic contractions in small intact fibre bundles (5-10 fibres) isolated from a fast foot muscle (flexor hallucis brevis) of the rat. A rapid T-jump of 2-4 degrees C was induced by a 0.2 ms infr...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-005-4568-0
更新日期:2005-01-01 00:00:00
abstract::In mammalian ventricles, two myosin heavy chain (MHC) isoforms have been identified. Small animals express α-MHC, whereas large animals express β-MHC, which contribute to a large difference in the heart rate. Sprague-Dawley rats possessing ~99% α-MHC were treated with propylthiouracil to result in 100% β-MHC. Papillar...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-016-9456-2
更新日期:2016-12-01 00:00:00
abstract::Myosin binding protein C (MYBPC) is a crucial component of the sarcomere and an important regulator of muscle function. While mutations in different myosin binding protein C (MYBPC) genes are well known causes of various human diseases, such as hypertrophic (HCM) and dilated (DCM) forms of cardiomyopathy as well as sk...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-011-9281-6
更新日期:2012-05-01 00:00:00
abstract::Nebulin is an integral protein of skeletal muscle thin filaments and probably acts as a ruler for the thin filament length. Cardiac muscles of higher vertebrates have been shown earlier to lack nebulin. Instead in human and chicken cardiac muscle the much smaller protein nebulette replaces nebulin. Since nebulette is ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1020909902462
更新日期:2002-01-01 00:00:00
abstract::The remodelling of connective tissue components is a fundamental requirement for a number of pivotal processes in cell biology. These may include myoblast migration and fusion during development and regeneration. In other systems, similar biological processes are facilitated by secretion of the matrix metalloproteinas...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005670507906
更新日期:2000-04-01 00:00:00
abstract::Rigor complexes between actin and myosin have been shown to cause increased binding of Ca2+ to troponin C. A similar effect of force-generating crossbridges has been suggested as an explanation for the coupling between load and activation which has been observed in skeletal and cardiac muscle. The goal of this study w...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00712584
更新日期:1985-08-01 00:00:00
abstract::The loss of muscle force and power during fatigue from intense contractile activity is associated with, and likely caused by, elevated levels of phosphate ([Formula: see text]) and hydrogen ions (decreased pH). To understand how these deficits in muscle performance occur at the molecular level, we used direct measurem...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-018-9499-7
更新日期:2018-08-01 00:00:00
abstract::We have determined the amino acid sequences of the essential light chains (ELC) and regulatory light chains (RLC) of myosin from two species of clam, Mercenaria mercenaria and Macrocallista nimbosa, using protein chemistry methods. The N-termini of all four proteins were blocked, and sequencing was carried out on vari...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738587
更新日期:1991-08-01 00:00:00
abstract::Heat rate and tension were measured during the steady state of isovelocity shortening in frog sartorius muscles at two speeds (7.5 and 2.0 mm s-1) through two ranges of the length-tension curve: 1.11 to 1.01 L0 and 1.01 to 0.91 L0. Both heat rate and mechanical power were higher at short length than at long length. Th...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01753412
更新日期:1986-04-01 00:00:00
abstract::It has been proposed that in striated muscle inositol 1,4,5-trisphosphate (InsP3) may serve as a chemical transmitter linking membrane depolarization to Ca(2+)-release from the sarcoplasmic reticulum. Key to that hypothesis of excitation-concentration (EC) coupling was the observation that skinned muscle fibres contra...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738039
更新日期:1992-08-01 00:00:00
abstract::The width (D) and the 1,0 lattice spacing (d1,0) at various ionic compositions of mechanically skinned single fibres (from semitendinosus muscle of Rana catesbeiana) were measured at various concentrations of polyvinyl pyrrolidone (PVP K-30, Mn = 40 000) from 0 to 6% at 20 degrees C. In a standard relaxing solution (4...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01753558
更新日期:1986-06-01 00:00:00
abstract::Skeletal muscle has a remarkable ability to respond to different physical stresses. Loading muscle through exercise, either anaerobic or aerobic, can lead to increases in muscle size and function while, conversely, the absence of muscle loading stimulates rapid decreases in size and function. A principal mediator of t...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-015-9415-3
更新日期:2015-10-01 00:00:00
abstract::A high resolution, time efficient method is described for determining the complex modulus of activated striated muscle. In this method the applied oscillation of muscle length takes the form of pseudo-random binary noise, PRBN. As a time-domain signal, PRBN is an extension of the double step approach to include n step...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01753650
更新日期:1986-08-01 00:00:00
abstract::Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscl...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-016-9441-9
更新日期:2016-04-01 00:00:00
abstract::While the migration of capillary endothelial cells is believed central to the process of new blood vessel development in vivo, the biochemical basis for endothelial motility is unknown. Herein, we demonstrate that retina-derived growth factor (RDGF), a mitogen for endothelial cells (EC), stimulates the migration of mi...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00713928
更新日期:1984-12-01 00:00:00
abstract::We describe the modification of reactive actin sulfhydryls by S-nitrosoglutathione. Kinetics of S-nitrosylation and denitrosylation suggest that only one cysteine of actin is involved in the reactions. By using the bifunctional sulfhydryl cross-linking reagent N,N'-1,4-phenylenebismaleimide and the monofunctional reag...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005671319604
更新日期:2000-02-01 00:00:00
abstract::Two procedures to inhibit Ca(2+) release designed to differentiate between local and common pool mechanisms for the Ca(2+) dependent, fast inactivation of Ca(2+) release in skeletal muscle of the frog were compared. Inhibition by voltage dependent inactivation of Ca(2+) release, without modification of the single chan...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-010-9212-y
更新日期:2010-08-01 00:00:00
abstract::Isolated frog muscle fibres of four different lengths (L0, initial; L1, 15%; L2, 25%; and L3, 35% stretched vs. L0) were stimulated continuously (3 min) by suprathreshold rectangular pulses at three different frequencies (f1 = 5 Hz, f2 = 6.7 Hz and f3 = 10 Hz). Using a pair of electrodes mounted at a fixed distance, t...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Two of the most characteristic features of striated muscle are (i) its ability to contract and generate tension when activated and (ii) its ability to return to its original length and form after contraction or stretching ceases. These two properties are to a large extent the primary manifestations of separate sets of...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/BF00123477
更新日期:1994-06-01 00:00:00
abstract::The mechanical events following release of ATP from P3-1-(2-nitro)phenylethyladenosine-5'-triphosphate (caged-ATP) in skinned guinea pig taenia coli smooth muscle in rigor were investigated. A rigor force of about 25-35% of the maximal active force was obtained by removing ATP at the plateau of a maximal active contra...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01578427
更新日期:1987-10-01 00:00:00
abstract::White muscle fibres from dogfish were used to compare the energetic costs of shortening by fully active muscle and by relaxing muscle. The muscle preparation was tetanized for 0.6 s and shortened either during stimulation or during relaxation. The distance shortened was 1 mm (about 15% L0, the muscle length optimum fo...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005455505056
更新日期:1998-10-01 00:00:00