Abstract：
:CapZ is a widely distributed and highly conserved, heterodimeric protein, that nucleates actin polymerization and binds to the barbed ends of actin filaments, preventing the addition or loss of actin monomers. CapZ interaction with actin filaments was shown to be of high affinity and decreased in the presence of PIP2. CapZ was located in nascent Z-lines during skeletal muscle myofibrillogenesis before the striated appearance of thin filaments in sarcomers. In this study, the stabilization and the anchorage of thin filaments were explored through identification of CapZ partners in the Z-line. Fish (sea bass) striated white muscle and its related Z-line proteins were selected since they correspond to the simplest Z-line organization. We report here the interaction between purified CapZ and alpha-actinin, a major component of Z filaments and polar links in Z-discs. Affinity of CapZ for alpha-actinin, estimated by fluorescence and immunochemical assays, is in the microM range. This association was found to be independent of actin and shown to be weakened in the presence of phosphoinositides. Binding contacts on the alpha-actinin molecule lie in the 55 kDa repetitive domain. A model including CapZ/alpha-actinin/titin/actin interactions is proposed considering Luther's 3D Z-line reconstruction.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Papa I,Astier C,Kwiatek O,Raynaud F,Bonnal C,Lebart MC,Roustan C,Benyamin Ydoi
10.1023/a:1005489319058keywords:
subject
Has Abstractpub_date
1999-02-01 00:00:00pages
187-97issue
2eissn
0142-4319issn
1573-2657journal_volume
20pub_type
杂志文章abstract：:Isolated fibres from various muscles were skinned mechanically in oil. From a Ca2+-loaded micropipette, local applications of Ca2+ were made. These produced a limited contraction which relaxed spontaneously. The time-course of sarcomere shortening and re-lengthening was recorded by microcinephotography. Application of...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018603032590
更新日期：1997-08-01 00:00:00
abstract：:The optimal utilization of energy substrates in muscle fibers is of primary importance for muscle contraction and whole body physiology. This study aimed to investigate the age-related changes in some indicators of glucose catabolism and fatty acid oxidation in muscles of growing rabbits. Longissimus lumborum (fast-tw...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-004-2768-7
更新日期：2004-01-01 00:00:00
abstract：:Calcium overload is a fundamental pathogenic event associated with chronic muscle degeneration in muscular dystrophies. The possibility that L-type voltage-dependent calcium channels were involved in the etiology of chicken muscular dystrophy was investigated by studying the dihydropyridine receptors in transverse tub...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00126437
更新日期：1995-10-01 00:00:00
abstract：:The effects of two amino acids, arginine which has a positively charged side-chain and glutamate which has a negatively charged side-chain on the Ca2+-activation properties of the contractile apparatus were examined in four structurally and functionally different types of skeletal muscle; long- and short-sarcomere fib...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-004-2769-6
更新日期：2004-01-01 00:00:00
abstract：:Proteins containing disintegrin domains play a variety of roles in regulating processes involving adhesion, migration and cell type specification during development of many metazoan organisms. Most disintegrin domain containing proteins belong to the ADAM (a disintegrin and a metalloprotease) family of proteins that a...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1023/a:1024440014857
更新日期：2002-01-01 00:00:00
abstract：:Guinea-pig soleus muscles were denervated and electrically stimulated for periods of 43 to 66 days. Stimuli were in 1 s bursts of 40 Hz pulses, repeated every 5 min. Other guinea-pigs were denervated for 82 days without stimulation and, in a third group, the soleus muscle was necrotized and allowed to regenerate witho...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018619503456
更新日期：1997-10-01 00:00:00
abstract：:The efficacy of the 'EGTA-treatment' for producing a model of selectively 'skinned' cardiac muscle has been questioned. This paper deals with ultrastructural evidence designed to test whether small ions can gain access to the myofibrillar space of the heart after 'EGTA-treatment'. Lanthanum has been employed because o...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711913
更新日期：1985-10-01 00:00:00
abstract：:The developmental expression of nebulin was studied in embryonic chick skeletal muscle cells in vitro by means of immunofluorescence microscopy. Initially nebulin appeared homogeneously or in a punctate form in the cytoplasm, and then it was assembled into I-Z-I-like complexes containing actin and alpha-actinin but no...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738427
更新日期：1992-02-01 00:00:00
abstract：:We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in both ATPase and motility assays. We demonstrate that skeletal muscle myosin (S...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-012-9316-7
更新日期：2012-12-01 00:00:00
abstract：:Skeletal muscle hypertrophy is a result of increased load, such as functional and stretch-overload. Activation of satellite cells and proliferation, differentiation and fusion are required for hypertrophy of overloaded skeletal muscles. On the contrary, a dramatic loss of skeletal muscle mass determines atrophy settin...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/s10974-010-9204-y
更新日期：2009-12-01 00:00:00
abstract：:In this study alterations are characterized which occur, in myocardial force development morphological appearance and protein composition, during the development of cardiac hypertrophy and heart failure in monocrotaline (MCT) treated rats. The transition from cardiac hypertrophy to heart failure was studied by compari...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1019988815436
更新日期：2002-01-01 00:00:00
abstract：:Numerous biological processes, including muscular contraction, depend upon the mechanical properties of actin filaments. One such property is resistance to bending (flexural rigidity, EI). To estimate EI, we attached the ends of fluorescently labelled actin filaments to two microsphere 'handles' captured in independen...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1018672631256
更新日期：1997-02-01 00:00:00
abstract：:The organization of the principal cytoskeletal components (actin, tubulin and 10 nm filament protein) have been compared by immunofluorescence microscopy in two populations of chick heart fibroblasts, previously shown to be adapted respectively for rapid, directed migration or adhesion and growth. We find that neither...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711922
更新日期：1980-03-01 00:00:00
abstract：:The five possible analogues of ATP and the three possible analogues of ADP which contain single non-bridging sulphur atoms instead of oxygen in the polyphosphate structure have been used as probes of the interaction of nucleotides with myosin and actomyosin. Evidence is presented for the requirement of an alpha, beta,...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711928
更新日期：1980-03-01 00:00:00
abstract：:Much has been written about the effects of aging on reproductive function, especially female fertility. Much less is known about how aging may affect the contractility of the smooth muscle within the uterus, the myometrium. The myometrium is active through a woman's entire life, not just during pregnancy. Here we will...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-012-9300-2
更新日期：2012-08-01 00:00:00
abstract：:The remodelling of connective tissue components is a fundamental requirement for a number of pivotal processes in cell biology. These may include myoblast migration and fusion during development and regeneration. In other systems, similar biological processes are facilitated by secretion of the matrix metalloproteinas...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005670507906
更新日期：2000-04-01 00:00:00
abstract：:The mRNA levels of the adult and the neonatal sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPases (SERCA1a and SERCA1b, respectively) and those of the muscle regulatory factors (MRFs: myoD, myf-5, myogenin, MRF4) have been assessed by RT PCR in rat soleus muscles immobilized for 3 days in an extended position (passive s...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005541522599
更新日期：1999-05-01 00:00:00
abstract：:Troponin T (TnT) is one of the muscle regulatory proteins and is thought to be related to unique contractile properties in diverse muscles and also to myogenesis. The expression of TnT isoforms in the chicken wing muscles was investigated by two-dimensional gel electrophoresis and immunoblotting with an antiserum agai...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1016369300532
更新日期：2001-01-01 00:00:00
abstract：:Using a variety of techniques, we have demonstrated the presence of at least two fibre types in Limulus median telson levator muscle. By light and electron microscopy, large (2,156 microns 2 mean cross-sectional area) fibres have A-bands of 4.1 microns, one-half I bands of 2.15 microns and Z lines less than or equal t...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01739856
更新日期：1989-02-01 00:00:00
abstract：:The effect of Ca2+ activation on the apparent rate constant governing the dissociation of force generating myosin crossbridges was studied in skinned rabbit adductor magnus fibres (fast-twitch) at 21 +/- 1 degree C. Simultaneous measurements of Ca2(+)-activated isometric force and ATPase activity were conducted in par...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01781174
更新日期：1991-02-01 00:00:00
abstract：:Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscl...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-016-9441-9
更新日期：2016-04-01 00:00:00
abstract：:A method for quick-freezing muscles while observing their mechanical properties until the moment of freezing is described. This method was used to freeze the anterior byssus retractor muscle (ABRM) of Mytilus edulis. Intact muscle in the presence of sucrose as a cryoprotectant was freeze-substituted in acetone, fixed ...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01758426
更新日期：1989-08-01 00:00:00
abstract：:A set of five differential equations has been found which gives a satisfactory account of the isotonic and isometric properties of striated muscle. Four of these differential equations give an equally satisfactory account of the results of length-drive experiments with sinusoidal variation of length. In this case, the...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章,评审
doi：10.1007/BF00713256
更新日期：1984-10-01 00:00:00
abstract：:The fine structure of leech body wall muscles, has been analysed under great length variations. All the measurable parameters of the fibre (distance between thick filaments, sarcomere and A band width, percentage of actin crowns around myosin filaments, fibre radius) at different elongations were tested with equations...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF00711915
更新日期：1985-10-01 00:00:00
abstract：:The effect of urocortin (Uro), a recently discovered neuropeptide with selectivity towards corticotropin-releasing hormone type 2 receptor, was tested on whole cell currents expressed by guinea-pig gastric antrum smooth muscle cells. Uro (1 pmol/l-1 nmol/l) caused a concentration-dependent increase of Ca2+-sensitive K...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1023/a:1005653218639
更新日期：2000-01-01 00:00:00
abstract：:The time course of shortening at zero load was studied by the slack test method during tetanic contractions in isolated, single, slow muscle fibres of the crayfish. In 28 of 32 shortenings (from 14 different fibres) a biphasic shortening was seen, which consisted of an initial high-velocity phase lasting 3.3-20.8 ms a...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01738251
更新日期：1992-12-01 00:00:00
abstract：:Although a substantial literature exists on the in vitro polymerization of purified myosin, little is known about native thick filament assembly, remodeling or turnover. We have recently described a cell-free system (Bouche et al., 1988) to examine the interactions between thick filaments and soluble, newly synthesize...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01774035
更新日期：1991-04-01 00:00:00
abstract：:This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+ concentration from the peak active stress during the isometric contraction. The active stress was computed from a me...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-011-9260-y
更新日期：2011-11-01 00:00:00
abstract：:Ryanodine receptors (RyRs) are huge homotetrameric Ca2+ release channels localized to the sarcoplasmic reticulum. RyRs are responsible for the release of Ca2+ from the SR during excitation-contraction coupling in striated muscle cells. Recent revolutionary advancements in cryo-electron microscopy have provided a numbe...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/s10974-020-09575-6
更新日期：2020-02-10 00:00:00
abstract：:Five monoclonal antibodies that react with different regions of myosin light chain 1 from human ventricular myocardial muscle were used to obtain information on interactions between the light chain 1 and heavy chains and generally on the tertiary structure of the light chain 1 within the myosin head. We performed West...
journal_title：Journal of muscle research and cell motility
pub_type： 杂志文章
doi：10.1007/BF01766461
更新日期：1992-06-01 00:00:00
