Effects of AMPPNP and vanadate on the mechanochemical crossbridge cycle in flagella.

abstract：:The regulated assembly of actin filaments is essential in nearly all cell types. Studying actin assembly dynamics can pose many technical challenges. A number of these challenges can be overcome by using microfluidics to observe and manipulate single actin filaments under an optical microscope. In particular, microflu...

journal_title：Journal of muscle research and cell motility

authors： Wioland H,Suzuki E,Cao L,Romet-Lemonne G,Jegou A

更新日期：2020-03-01 00:00:00

abstract：:Our current understanding of the structure of the thin filaments of muscle and the molecular mechanism by which thin filaments regulate muscle contraction are reviewed and discussed. We focus, in particular, on the crucial role played by Jean Hanson in these studies and on later contributions from those whose work she...

journal_title：Journal of muscle research and cell motility

authors： Lehman W,Craig R

更新日期：2004-01-01 00:00:00

abstract：:Myosin heavy chain kinase activity was identified in the high speed supernate of lysed Dictyostelium amoebae and was precipitated by 30-50% ammonium sulphate. In low ionic strength buffer, the activity bound tightly to a Cibacron Blue Sepharose column and eluted as a single peak with 1.0 M NaCl. Gel filtration chromat...

journal_title：Journal of muscle research and cell motility

authors： Kuczmarski ER

更新日期：1986-12-01 00:00:00

abstract：:Myosin has been purified from chicken pectoralis muscle at various stages of development, from 10 days' incubation to approximately 10 months after hatching. Embryonic myosin from the earliest stage showed a high level of ATPase activity, similar to that obtained for adult pectoralis myosin. Two-dimensional peptide ma...

journal_title：Journal of muscle research and cell motility

authors： Lowey S,Benfield PA,LeBlanc DD,Waller GS

更新日期：1983-12-01 00:00:00

abstract：:This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+ concentration from the peak active stress during the isometric contraction. The active stress was computed from a me...

journal_title：Journal of muscle research and cell motility

authors： Li WG,Luo XY,Hill NA,Ogden RW,Tian TH,Smythe A,Majeed AW,Bird N

更新日期：2011-11-01 00:00:00

abstract：:Critical illness myopathy (CIM) is associated with severe muscle atrophy and fatigue in affected patients. Apoptotic signaling is involved in atrophy and is elevated in muscles from patients with CIM. In this study we investigated underlying mechanisms of apoptosis-related pathways in muscles with different fiber type...

journal_title：Journal of muscle research and cell motility

authors： Barnes BT,Confides AL,Rich MM,Dupont-Versteegden EE

更新日期：2015-06-01 00:00:00

abstract：:The pattern of spontaneous skeletal muscle degeneration and clinical recovery hindlimb muscles of the mdx mutant mouse was examined for functional and metabolic confirmation of apparent structural regeneration. The contractile properties, histochemical staining and myosin light chain and parvalbumin contents of extens...

journal_title：Journal of muscle research and cell motility

authors： Anderson JE,Bressler BH,Ovalle WK

更新日期：1988-12-01 00:00:00

abstract：:CapZ is a widely distributed and highly conserved, heterodimeric protein, that nucleates actin polymerization and binds to the barbed ends of actin filaments, preventing the addition or loss of actin monomers. CapZ interaction with actin filaments was shown to be of high affinity and decreased in the presence of PIP2....

journal_title：Journal of muscle research and cell motility

authors： Papa I,Astier C,Kwiatek O,Raynaud F,Bonnal C,Lebart MC,Roustan C,Benyamin Y

更新日期：1999-02-01 00:00:00

abstract：:Skeletal muscle myofibers constantly undergo degeneration and regeneration. Histopathological features of 6 skeletal muscles (cranial tibial [CT], gastrocnemius, quadriceps femoris, triceps brachii [TB], lumbar longissimus muscles, and costal part of the diaphragm [CPD]) were compared using C57BL/10ScSn-Dmd mdx (mdx) ...

journal_title：Journal of muscle research and cell motility

authors： Ikeda T,Ichii O,Otsuka-Kanazawa S,Nakamura T,Elewa YH,Kon Y

更新日期：2016-10-01 00:00:00

abstract：:Filamin binds to F-actin and influences the myosin-actin interaction. At relatively low concentrations, filamin activates actomyosin Mg2+-ATPase, whereas higher concentrations of filamin exert an inhibitory effect. Activation of ATPase activity occurs under conditions where a loose meshwork of actin filaments is prese...

journal_title：Journal of muscle research and cell motility

authors： Dabrowska R,Goch A,Osińska H,Szpacenko A,Sosinski J

更新日期：1985-02-01 00:00:00

abstract：:The sonic muscle of the oyster toadfish, Opsanus tau, can produce unfused contractions at 300 Hz. Electron microscopy shows a great abundance of the Sarcoplasmic reticulum (SR) in this muscle, but no functional characterization of the capabilities of the SR has been reported. We measured the oxalate-supported Ca2+ upt...

journal_title：Journal of muscle research and cell motility

authors： Feher JJ,Waybright TD,Fine ML

更新日期：1998-08-01 00:00:00

abstract：:We have determined the amino acid sequences of the essential light chains (ELC) and regulatory light chains (RLC) of myosin from two species of clam, Mercenaria mercenaria and Macrocallista nimbosa, using protein chemistry methods. The N-termini of all four proteins were blocked, and sequencing was carried out on vari...

journal_title：Journal of muscle research and cell motility

authors： Barouch WW,Breese KE,Davidoff SA,Leszyk J,Szent-Györgyi AG,Theibert JL,Collins JH

更新日期：1991-08-01 00:00:00

abstract：:Single twitch muscle fibres have been isolated from various parts of the iliofibularis muscle of Xenopus laevis. After measurements of their isotonic contractile properties, myosin extraction was performed on individual fibres and the extract analysed by various forms of gel electrophoresis. In agreement with previous...

journal_title：Journal of muscle research and cell motility

authors： Lännergren J

更新日期：1987-06-01 00:00:00

abstract：:Trabeculae from the right ventricle of rat were chemically 'skinned' with saponin (50 micrograms ml-1 for 30 min). Caffeine (10 mM) induced a transient contracture as a consequence of Ca(2+)-release from the sarcoplasmic reticulum and subsequent activation of the myofilaments. The amplitude of the caffeine contracture...

journal_title：Journal of muscle research and cell motility

authors： Steele DS,Miller DJ

更新日期：1992-04-01 00:00:00

abstract：:We have investigated high-affinity ryanodine-binding sites in membrane preparations from representative fast-twitch and slow-twitch muscles of the rabbit and rat, as well as from human mixed muscle. Our results, obtained in high-ionic strength binding buffer, demonstrate extensive similarities in binding affinity for ...

journal_title：Journal of muscle research and cell motility

authors： Damiani E,Margreth A

更新日期：1994-04-01 00:00:00

abstract：:The time course of shortening at zero load was studied by the slack test method during tetanic contractions in isolated, single, slow muscle fibres of the crayfish. In 28 of 32 shortenings (from 14 different fibres) a biphasic shortening was seen, which consisted of an initial high-velocity phase lasting 3.3-20.8 ms a...

journal_title：Journal of muscle research and cell motility

authors： Tameyasu T

更新日期：1992-12-01 00:00:00

abstract：:In Drosophila melanogaster two high molecular weight tropomyosin isoforms, historically named heavy troponins (TnH-33 and TnH-34), are encoded by the Tm1 tropomyosin gene. They are specifically expressed in the indirect flight muscles (IFM). Their N-termini are conventional and complete tropomyosin sequences, but thei...

journal_title：Journal of muscle research and cell motility

authors： Mateos J,Herranz R,Domingo A,Sparrow J,Marco R

更新日期：2006-01-01 00:00:00

abstract：:Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscl...

journal_title：Journal of muscle research and cell motility

authors： Murach KA,Bagley JR,McLeland KA,Arevalo JA,Ciccone AB,Malyszek KK,Wen Y,Galpin AJ

更新日期：2016-04-01 00:00:00

abstract：:Using a variety of techniques, we have demonstrated the presence of at least two fibre types in Limulus median telson levator muscle. By light and electron microscopy, large (2,156 microns 2 mean cross-sectional area) fibres have A-bands of 4.1 microns, one-half I bands of 2.15 microns and Z lines less than or equal t...

journal_title：Journal of muscle research and cell motility

authors： Levine RJ,Davidheiser S,Kelly AM,Kensler RW,Leferovich J,Davies RE

更新日期：1989-02-01 00:00:00

abstract：:The pattern of expression of different types of myosin heavy chains and the development of different generations of muscle cells during foetal life were studied in four bovine muscles with widely varying characteristics, the Masseter, Longissimus thoracis, Cutaneus trunci and Diaphragma. Different complementary techni...

journal_title：Journal of muscle research and cell motility

authors： Picard B,Robelin J,Pons F,Geay Y

更新日期：1994-08-01 00:00:00

abstract：:It is generally thought that the rapid relaxation of fast muscles is facilitated by the Ca(2+) binding protein parvalbumin (Parv). Indeed superfast swimbladder (SWB) muscle of toadfish contains the largest concentration of this protein ever observed (up to 1.5 mM). At 15 degrees C toadfish perform a 100 Hz call, 400 m...

journal_title：Journal of muscle research and cell motility

authors： Tikunov BA,Rome LC

更新日期：2009-01-01 00:00:00

abstract：:The fine structure of leech body wall muscles, has been analysed under great length variations. All the measurable parameters of the fibre (distance between thick filaments, sarcomere and A band width, percentage of actin crowns around myosin filaments, fibre radius) at different elongations were tested with equations...

journal_title：Journal of muscle research and cell motility

authors： Lanzavecchia G,de Eguileor M,Valvassori R

更新日期：1985-10-01 00:00:00

abstract：:Muscle development involves a complex sequence of time and spatially regulated cellular events leading to the formation of highly specialised syncytial muscle cells displaying a common feature, the capacity of contraction. Analyses of mechanisms controlling muscle development reveals that the main steps of muscle form...

journal_title：Journal of muscle research and cell motility

authors： Maqbool T,Jagla K

更新日期：2007-01-01 00:00:00

abstract：:The length dependency of the sensitivity to activators of the smooth muscle of different blood vessels is not yet fully understood. Muscle preparations of the aorta, the femoral artery and the portal vein of the rabbit were investigated for the length dependency of the sensitivity to phenylephrine and calcium in both ...

journal_title：Journal of muscle research and cell motility

authors： Van Heijst BG,De Wit E,Van der Heide UA,Blangé T,Jongsma HJ,De Beer EL

更新日期：1999-01-01 00:00:00

abstract：:The developmental expression of nebulin was studied in embryonic chick skeletal muscle cells in vitro by means of immunofluorescence microscopy. Initially nebulin appeared homogeneously or in a punctate form in the cytoplasm, and then it was assembled into I-Z-I-like complexes containing actin and alpha-actinin but no...

journal_title：Journal of muscle research and cell motility

authors： Komiyama M,Zhou ZH,Maruyama K,Shimada Y

更新日期：1992-02-01 00:00:00

abstract：:We examined the temperature dependence of muscle and non-muscle myosin (heavy meromyosin, HMM) with in vitro motility and actin-activated ATPase assays. Our results indicate that myosin V (MV) has a temperature dependence that is similar in both ATPase and motility assays. We demonstrate that skeletal muscle myosin (S...

journal_title：Journal of muscle research and cell motility

authors： Yengo CM,Takagi Y,Sellers JR

更新日期：2012-12-01 00:00:00

abstract：:The β-adrenergic receptor stimulation improves endurance in fast twitch muscles and these effects are sensitive to extracellular Ca2+ influx. Present study is aimed to determine the effects of adrenaline, with different concentrations of extracellular Ca2+[Formula: see text], on the contractility and endurance of slow...

journal_title：Journal of muscle research and cell motility

authors： Rizvi MH,Abdul Azeem M,Savanur A

更新日期：2019-12-01 00:00:00

abstract：:The myosin II motor from Dictyostelium discoideum has been engineered to contain single tryptophan residues at strategic locations to probe movements of switch 1 and switch 2. The tryptophan residue at W501 probes movement of the relay helix and indirectly reports on switch 2 movement. This probe suggests that there i...

journal_title：Journal of muscle research and cell motility

authors： Málnási-Csizmadia A,Dickens JL,Zeng W,Bagshaw CR

更新日期：2005-01-01 00:00:00

abstract：:Stretchin-klp is a newly described protein in Drosophila indirect flight muscles (IFM) that migrates on SDS gels as two distinct components of approximately 225 and 231 kD. Although the larger isoform is IFM specific, the smaller stretchin-klp isoform is expressed not only in IFM, but also in wild-type tissues of the ...

journal_title：Journal of muscle research and cell motility

authors： Patel SR,Saide JD

更新日期：2005-01-01 00:00:00

abstract：:Heart disease is a leading cause of death in patients with Duchenne muscular dystrophy (DMD). Patients with DMD lack the protein dystrophin, which is widely expressed in striated muscle. In skeletal muscle, the loss of dystrophin results in dramatically decreased expression of the dystrophin associated glycoprotein co...

journal_title：Journal of muscle research and cell motility

authors： Sharpe KM,Premsukh MD,Townsend D

更新日期：2013-12-01 00:00:00