Abstract:
:The regulated assembly of actin filaments is essential in nearly all cell types. Studying actin assembly dynamics can pose many technical challenges. A number of these challenges can be overcome by using microfluidics to observe and manipulate single actin filaments under an optical microscope. In particular, microfluidics can be tremendously useful for applying different mechanical stresses to actin filaments and determining how the physical context of the filaments affects their regulation by biochemical factors. In this review, we summarize the main features of microfluidics for the study of actin assembly dynamics, and we highlight some recent developments that have emerged from the combination of microfluidics and other techniques. We use two case studies to illustrate our points: the rapid assembly of actin filaments by formins and the disassembly of filaments by actin depolymerizing factor (ADF)/cofilin. Both of these protein families play important roles in cells. They regulate actin assembly through complex molecular mechanisms that are sensitive to the filaments' mechanical context, with multiple activities that need to be quantified separately. Microfluidics-based experiments have been extremely useful for gaining insight into the regulatory actions of these two protein families.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Wioland H,Suzuki E,Cao L,Romet-Lemonne G,Jegou Adoi
10.1007/s10974-019-09564-4subject
Has Abstractpub_date
2020-03-01 00:00:00pages
175-188issue
1eissn
0142-4319issn
1573-2657pii
10.1007/s10974-019-09564-4journal_volume
41pub_type
杂志文章abstract::Long-(SL > 6 microns) and short-sarcomere (SL < 4 microns) fibres were isolated from the claw muscle of the yabby (Cherax destructor) during limb regeneration and at different stages of the moult cycle. Long-sarcomere fibres were more susceptible to the changes resulting from the moult-induced atrophy compared with th...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00121136
更新日期:1995-06-01 00:00:00
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journal_title:Journal of muscle research and cell motility
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005671319604
更新日期:2000-02-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00711879
更新日期:1982-03-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1014543507149
更新日期:2001-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/b:jure.0000035879.87045.4b
更新日期:2004-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-012-9300-2
更新日期:2012-08-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00130240
更新日期:1995-12-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00711961
更新日期:1983-02-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1013188001776
更新日期:2001-01-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00713154
更新日期:1984-02-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00115451
更新日期:1993-04-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005585807179
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00140322
更新日期:1996-02-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01774035
更新日期:1991-04-01 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-010-9204-y
更新日期:2009-12-01 00:00:00
abstract::The developmental expression of nebulin was studied in embryonic chick skeletal muscle cells in vitro by means of immunofluorescence microscopy. Initially nebulin appeared homogeneously or in a punctate form in the cytoplasm, and then it was assembled into I-Z-I-like complexes containing actin and alpha-actinin but no...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738427
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journal_title:Journal of muscle research and cell motility
pub_type: 传,历史文章,杂志文章
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journal_title:Journal of muscle research and cell motility
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更新日期:2020-02-10 00:00:00
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journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00711869
更新日期:1981-06-01 00:00:00
abstract::Cytokinesis is the mechanical process that allows the simplest unit of life, the cell, to divide, propagating itself. To divide, the cell converts chemical energy into mechanical energy to produce force. This process is thought to be active, due in large part to the mechanochemistry of the myosin-II ATPase. The cell's...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1023/a:1024419510314
更新日期:2002-01-01 00:00:00