Abstract:
:The giant molecule titin (also called connectin) provides an elastic connection in the I-band between the Z-disk and A-band of striated muscle. This region is assembled in a tissue-specific way by extensive differential splicing events. We have raised monoclonal antibodies against the two N2-line isoforms of titin and demonstrate that both forms of cardiac I-band titin are constitutively co-expressed in atrial and ventricular muscle. In developing mouse embryos, the expression of the cardiac N2-B isoform remains strictly cardiac-specific and is linked to the expression of the ubiquitous N2-A isoform. The mechanical function of the cardiac N2-line region was investigated ultrastructurally. Immunoelectron microscopy reveals that the N2-B region separates two mechanically distinct sections of titin with a hyperextensible segment spanning the distance to the Z-disk. The formation of a plateau in the extension of cardiac titin rules out that Ig-domains can be unfolded as a mechanism of elasticity.
journal_name
J Muscle Res Cell Motiljournal_title
Journal of muscle research and cell motilityauthors
Gautel M,Lehtonen E,Pietruschka Fdoi
10.1007/BF00123361subject
Has Abstractpub_date
1996-08-01 00:00:00pages
449-61issue
4eissn
0142-4319issn
1573-2657journal_volume
17pub_type
杂志文章abstract::The aim of the present study was to examine the temperature dependence of active force in lengthening and shortening muscle. Experiments were done, in vitro, on bundles of intact fibres (fibre length L(0) ~2 mm; sarcomere length ~2.5 μm) isolated from a rat fast muscle (flexor hallucis brevis) and a ramp length change...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-012-9307-8
更新日期:2012-10-01 00:00:00
abstract::Long-(SL > 6 microns) and short-sarcomere (SL < 4 microns) fibres were isolated from the claw muscle of the yabby (Cherax destructor) during limb regeneration and at different stages of the moult cycle. Long-sarcomere fibres were more susceptible to the changes resulting from the moult-induced atrophy compared with th...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00121136
更新日期:1995-06-01 00:00:00
abstract::CapZ is a widely distributed and highly conserved, heterodimeric protein, that nucleates actin polymerization and binds to the barbed ends of actin filaments, preventing the addition or loss of actin monomers. CapZ interaction with actin filaments was shown to be of high affinity and decreased in the presence of PIP2....
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005489319058
更新日期:1999-02-01 00:00:00
abstract::The effects of a ramp stretch (amplitude <6% muscle fibre length (L0), speed < 13L0 s(-1)) on twitch tension and twitch tension re-development were examined in intact mammalian (rat) fast and slow muscle fibre bundles. The experiments were done in vitro at 20 degrees C and at an initial sarcomere length of 2.68 microm...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1010556623905
更新日期:2001-01-01 00:00:00
abstract::Cytokinesis is the mechanical process that allows the simplest unit of life, the cell, to divide, propagating itself. To divide, the cell converts chemical energy into mechanical energy to produce force. This process is thought to be active, due in large part to the mechanochemistry of the myosin-II ATPase. The cell's...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1023/a:1024419510314
更新日期:2002-01-01 00:00:00
abstract::The characteristics of the titin filament in the vicinity of the Z-line were investigated using immunoelectron microscopy. We used monoclonal titin antibodies T-11 and T-12 on single fibres of frog skeletal muscle, and on Z-line-extracted fibres. It is well established that the I-band region of titin is elastic. We fi...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00121293
更新日期:1993-08-01 00:00:00
abstract::Myosin heavy chain kinase activity was identified in the high speed supernate of lysed Dictyostelium amoebae and was precipitated by 30-50% ammonium sulphate. In low ionic strength buffer, the activity bound tightly to a Cibacron Blue Sepharose column and eluted as a single peak with 1.0 M NaCl. Gel filtration chromat...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01753566
更新日期:1986-12-01 00:00:00
abstract::Trabeculae from the right ventricle of rat were chemically 'skinned' with saponin (50 micrograms ml-1 for 30 min). Caffeine (10 mM) induced a transient contracture as a consequence of Ca(2+)-release from the sarcoplasmic reticulum and subsequent activation of the myofilaments. The amplitude of the caffeine contracture...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01874151
更新日期:1992-04-01 00:00:00
abstract::Nebulin is an integral protein of skeletal muscle thin filaments and probably acts as a ruler for the thin filament length. Cardiac muscles of higher vertebrates have been shown earlier to lack nebulin. Instead in human and chicken cardiac muscle the much smaller protein nebulette replaces nebulin. Since nebulette is ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1020909902462
更新日期:2002-01-01 00:00:00
abstract::Quantitative (muscle fibre number and cross-sectional areas) and qualitative (myosin isoforms and metabolic enzyme activities) characteristics of two muscles, M. pectoralis major and M. anterior latissimus dorsi, were compared among male chickens of two lines during growth from hatching to adulthood. The lines were de...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00122527
更新日期:1995-04-01 00:00:00
abstract::Using a variety of techniques, we have demonstrated the presence of at least two fibre types in Limulus median telson levator muscle. By light and electron microscopy, large (2,156 microns 2 mean cross-sectional area) fibres have A-bands of 4.1 microns, one-half I bands of 2.15 microns and Z lines less than or equal t...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01739856
更新日期:1989-02-01 00:00:00
abstract::Critical illness myopathy (CIM) is associated with severe muscle atrophy and fatigue in affected patients. Apoptotic signaling is involved in atrophy and is elevated in muscles from patients with CIM. In this study we investigated underlying mechanisms of apoptosis-related pathways in muscles with different fiber type...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-015-9410-8
更新日期:2015-06-01 00:00:00
abstract::A high resolution, time efficient method is described for determining the complex modulus of activated striated muscle. In this method the applied oscillation of muscle length takes the form of pseudo-random binary noise, PRBN. As a time-domain signal, PRBN is an extension of the double step approach to include n step...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01753650
更新日期:1986-08-01 00:00:00
abstract::The optimal utilization of energy substrates in muscle fibers is of primary importance for muscle contraction and whole body physiology. This study aimed to investigate the age-related changes in some indicators of glucose catabolism and fatty acid oxidation in muscles of growing rabbits. Longissimus lumborum (fast-tw...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-004-2768-7
更新日期:2004-01-01 00:00:00
abstract::The time course of shortening at zero load was studied by the slack test method during tetanic contractions in isolated, single, slow muscle fibres of the crayfish. In 28 of 32 shortenings (from 14 different fibres) a biphasic shortening was seen, which consisted of an initial high-velocity phase lasting 3.3-20.8 ms a...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738251
更新日期:1992-12-01 00:00:00
abstract::The developmental shift in contractile protein isoform expression in the rodent heart likely affects actin-myosin cross-bridge interactions. We compared the Ca2+ sensitivity for force generation and cross-bridge cycling kinetics in neonatal (postnatal days 0-3) and adult (day 84) rats. The force-pCa relationship was d...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005585807179
更新日期:1999-10-01 00:00:00
abstract::The present study describes transitions in myosin heavy chain expression in the extraocular muscles of rats between the ages of E17 and adult. The unique phenotype of the extraocular muscle is reflected in its fibre type composition, which is comprised by six distinct profiles, each defined by location (orbital versus...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00240928
更新日期:1996-06-01 00:00:00
abstract::We determined the effects of epigallocatechin-3-gallate (EGCG) and epicatechin (EC), on pump turnover and Ca2+ transport by the cardiac form of the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA). Fluorescence spectroscopy was used to directly measure SERCA ATPase activity and to measure Ca2+ uptake into cardia...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-011-9256-7
更新日期:2011-09-01 00:00:00
abstract::Skeletal muscle fibers exhibit plasticity of their physiological and biochemical properties in response to the firing pattern from the innervating motor neuron. In particular, the gene expression pattern generally characteristic of a slow twitch fiber can be induced in a fast twitch fiber by chronic slow fiber type el...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-006-9080-7
更新日期:2006-01-01 00:00:00
abstract::Cleavage of vertebrate muscle tropomyosin by bacterial Omp T produces an amino-terminally truncated product (residues 7-284). The proteolysed protein, which is resolved from the parent by electrophoresis in the presence of sodium dodecylsulphate, can be generated from a variety of striated and smooth muscle tropomyosi...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-007-9114-9
更新日期:2007-01-01 00:00:00
abstract::Muscle development involves a complex sequence of time and spatially regulated cellular events leading to the formation of highly specialised syncytial muscle cells displaying a common feature, the capacity of contraction. Analyses of mechanisms controlling muscle development reveals that the main steps of muscle form...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章,评审
doi:10.1007/s10974-008-9133-1
更新日期:2007-01-01 00:00:00
abstract::To date, there has been no report of rat TnC purification, despite the rat being an animal commonly used in physiological studies of mammalian muscle. In this study we isolated the fast and slow Troponin C isoforms from rat extensor digitorum longus (23 microg TnC/g wet weight) and soleus (17.6 microg TnC/g wet weight...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/b:jure.0000009896.78385.16
更新日期:2003-01-01 00:00:00
abstract::Cardiac muscle M-band structures in several mammals (guinea pig, rabbit, rat and cow) and also from three teleosts (plaice, carp and roach), have been studied using electron microscopy and image processing. Axial structure seen in negatively stained isolated myofibrils or negatively stained cryo-sections shows the pre...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF00121071
更新日期:1994-12-01 00:00:00
abstract::Two procedures to inhibit Ca(2+) release designed to differentiate between local and common pool mechanisms for the Ca(2+) dependent, fast inactivation of Ca(2+) release in skeletal muscle of the frog were compared. Inhibition by voltage dependent inactivation of Ca(2+) release, without modification of the single chan...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/s10974-010-9212-y
更新日期:2010-08-01 00:00:00
abstract::The sonic muscle of the oyster toadfish, Opsanus tau, can produce unfused contractions at 300 Hz. Electron microscopy shows a great abundance of the Sarcoplasmic reticulum (SR) in this muscle, but no functional characterization of the capabilities of the SR has been reported. We measured the oxalate-supported Ca2+ upt...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005333215172
更新日期:1998-08-01 00:00:00
abstract::Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement. In this paper we report the molecular characterization of the Drosophila myosin V, identified by reverse genetics. The gene encodes a 1792-residue, 207 kDa heavy chain polypeptide which p...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005356511634
更新日期:1998-02-01 00:00:00
abstract::The length dependency of the sensitivity to activators of the smooth muscle of different blood vessels is not yet fully understood. Muscle preparations of the aorta, the femoral artery and the portal vein of the rabbit were investigated for the length dependency of the sensitivity to phenylephrine and calcium in both ...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1005408015885
更新日期:1999-01-01 00:00:00
abstract::Troponin T (TnT) is one of the muscle regulatory proteins and is thought to be related to unique contractile properties in diverse muscles and also to myogenesis. The expression of TnT isoforms in the chicken wing muscles was investigated by two-dimensional gel electrophoresis and immunoblotting with an antiserum agai...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1016369300532
更新日期:2001-01-01 00:00:00
abstract::To explain observed differences in the activation dependence of force redevelopment kinetics between cardiac and skeletal muscle, two numerical models of contractile regulation by Ca2+ were investigated. Ca2+ binding and force production were each modelled as two-state processes with forward and reverse rate constants...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1023/a:1018635907091
更新日期:1997-12-01 00:00:00
abstract::It has been proposed that in striated muscle inositol 1,4,5-trisphosphate (InsP3) may serve as a chemical transmitter linking membrane depolarization to Ca(2+)-release from the sarcoplasmic reticulum. Key to that hypothesis of excitation-concentration (EC) coupling was the observation that skinned muscle fibres contra...
journal_title:Journal of muscle research and cell motility
pub_type: 杂志文章
doi:10.1007/BF01738039
更新日期:1992-08-01 00:00:00