Abstract：
:Cardiac hypertrophy is an independent risk factor in the development of heart failure. However, the cellular mechanisms underlying the transition from compensated hypertrophy to heart failure are incompletely understood. The aim of this study was to investigate changes in myocardial substrate utilisation and function in pressure-overload hypertrophy (using 13C NMR spectroscopy) in parallel with alterations in the expression pattern of genes involved in cardiac fatty acid and glucose uptake and oxidation. Left ventricular hypertrophy was induced surgically in Sprague-Dawley rats by inter-renal aortic constriction. Nine weeks later, hearts were perfused in the isovolumic mode with a physiological mixture of substrates including 5 mM 1-13C glucose, 1 mM 3-13C lactate, 0.1 mM U-13C pyruvate and 0.3 mM U-13C palmitate and cardiac function monitored simultaneously. Real-time PCR was used to determine mRNA levels of PPARalpha and PPARalpha-regulated metabolic enzymes. Results showed that at the stage of compensated hypertrophy, fatty acid oxidation (FAO) and expression of genes involved in FAO were markedly reduced, whilst pyruvate oxidation was enhanced, highlighting the fact that metabolic remodelling is an early event in the development of cardiac hypertrophy.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Akki A,Smith K,Seymour AMdoi
10.1007/s11010-008-9711-ysubject
Has Abstractpub_date
2008-04-01 00:00:00pages
215-24issue
1-2eissn
0300-8177issn
1573-4919journal_volume
311pub_type
杂志文章abstract：:Hepatocyte cell adhesion molecule (HepaCAM) plays a crucial role in tumor progression and has been recognized as a novel tumor suppressor gene. The high protein expression level of protein kinase Cε (PKCε) has been discovered in many tumor types. In the present study, we determined HepaCAM and PKCε protein levels in h...
journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：2003-09-01 00:00:00
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更新日期：2008-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：1994-02-09 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11010-013-1948-4
更新日期：2014-04-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：10.1023/a:1007016403736
更新日期：1999-12-01 00:00:00
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更新日期：1999-02-01 00:00:00
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