Abstract:
:Human telomerase reverse transcriptase (hTERT) is an attractive target for cancer gene therapy. However, the poor inhibition of telomerase and a time lag between the inhibition and arrest of cell proliferation limits its use in cancer gene therapy. RNA interference (RNAi) has been proposed as a potential technique for the treatment of cancer with a long-term gene silencing response induced by short hairpin RNA (shRNA). To investigate the long-term effects of telomerase inhibition through the down-regulation of the hTERT gene and the potential role of hTERT in cancer gene therapy, we constructed an shRNA-directed hTERT-expressing vector and introduced it into SGC-7901 cells. A population of cells that stably expressed the shRNA was selected by G418 and continuously cultured in a medium with half the antibiotic concentration for 3 months and the anti-proliferation effects of shRNA-targeted hTERT were then detected. The results showed that shRNA-targeted hTERT significantly inhibited cell proliferation and increased cell apoptosis by down-regulating hTERT expression, thus decreasing telomerase activity. These findings suggest that shRNA-targeted hTERT has long-term anti-proliferation effects on SGC-7901 cells, and it is a potential approach in telomerase-based gene therapy.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Qian X,Cheng J,Chen A,Wang Y,Tao Y,Cao J,Feng Zsubject
Has Abstractpub_date
2008-02-01 00:00:00pages
575-81issue
2eissn
1021-335Xissn
1791-2431journal_volume
19pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::An arsenical compound, As2O3 has been reported to be effective for treating acute leukemia and induce apoptosis in many different tumor cell types. In this study we designed a novel arsenical compound, As4O6, and compared its ability to induce cell growth inhibition as well as gene expression profiles along with As2O3...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Primary and metastatic cancers that affect bones are frequently associated with severe and intractable pain. The mechanisms underlying the development of bone cancer pain are largely unknown. In the present study, we investigated whether inhibition of KCNQ/M (Kv7) potassium channels in the spinal cord contributes to t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3718
更新日期:2015-03-01 00:00:00
abstract::microRNAs (miRNAs) are known to be involved in the pathogenesis of hepatocellular carcinoma (HCC). miR-128-3p was recently reported to be deregulated in several types of cancer. However, the biological function and potential mechanisms of miR-128-3p in HCC remain unknown. In the present study, we found that miR-128-3p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3936
更新日期:2015-06-01 00:00:00
abstract::Recently, overexpression of EphA2, a member of the Eph family of receptor tyrosine kinases, has been reported in several cancers. Reduced expression of E-cadherin, an intercellular adhesion molecule of epithelial cells, has been reported to be associated with aggressive clinicopathological phenotypes in various cancer...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::KIAA1199 is a gene included in the Human Unidentified Gene-Encoded (HUGE) large protein database which contains more than 2,400 members identified in the Kazusa cDNA sequencing project. Early studies described KIAA1199 as an inner ear-specific protein in which 3 point mutations were found to be associated with non‑syn...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2014.3038
更新日期:2014-04-01 00:00:00
abstract::Inhibitor of DNA-binding protein 1 (ID1) is commonly abnormally overexpressed in colorectal cancer (CRC); yet, the functional significance of ID1 in the growth and invasive properties of CRC cells remains largely unclear. The present study investigated the effects of ID1 downregulation on the cell growth and metastati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3172
更新日期:2014-07-01 00:00:00
abstract::Nucleostemin (NS) plays an important role in tumorigenesis and progression. Most studies consider that NS plays its role through combining with p53 and inhibiting it, however our previous studies revealed that NS could also function without the existence of p53. To date, few studies have focused on the p53-independent...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3240
更新日期:2014-08-01 00:00:00
abstract::MicroRNAs (miRNAs) play a vital role in tumour biological and pathologic processes. In the present study, we aimed to detect the expression and biological role of miR-216b in glioma. Our data showed that miR-216b was significantly downregulated in human glioma tissues and cells. Ectopic expression of miR-216b inhibite...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5824
更新日期:2017-09-01 00:00:00
abstract::The MSH6 gene is one of the mismatch repair genes involved in Lynch syndrome and its mutations account for 10-20% of Lynch syndrome. Although previous studies suggested that the difference of the geographical region affects the clinical phenotype of Lynch syndrome, there has been no report on the detailed features of ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2781
更新日期:2013-12-01 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most malignant intracranial tumor. Although the affected patients are usually treated with surgery combined with radiotherapy and chemotherapy, the median survival time for GBM patients is still approximately 12‑14 months. Identifying the key molecular mechanisms and targets of GBM...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7204
更新日期:2019-08-01 00:00:00
abstract::This study aimed to investigate the effect of RNA interference (RNAi)-mediated downregulation of the expression of the c-jun gene (a proto-oncogene) on the radiosensitivity of a radioresistant human nasopharyngeal carcinoma cell line (CNE-2R) and to validate its potential as an anticancer target. A lentiviral vector w...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3692
更新日期:2015-03-01 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most aggressive human brain cancer. Little is known regarding how these cells adapt to the harsh tumor microenvironment, and consequently survive and resist various treatments. Myoglobin (MB), the oxygen‑binding hemoprotein, has been shown to be ectopically expressed in different h...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7479
更新日期:2020-03-01 00:00:00
abstract::In order to erase reactive oxygen species (ROS) related with the proliferation of tumor cells by reducing activity of hydrogen, we developed functional water containing nano-bubbles (diameters: <900 nm for 71%/population) hydrogen of 1.1-1.5 ppm (the theoretical maximum: 1.6 ppm) with a reducing ability (an oxidation-...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00001006
更新日期:2010-12-01 00:00:00
abstract::Autophagy is an intracellular multi-step catabolic degradation process that involves the degradation and recycling of cellular proteins and cytoplasmic damaged organelles to maintain cellular homeostasis and reduction of metabolic stress. Numerous studies have indicated the importance of autophagy in cancer, but the r...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4302
更新日期:2015-12-01 00:00:00
abstract::We previously reported that chromosome arm 10q is one of the target regions of allelic loss in human endometrial cancer. To identify the gene in this region responsible for endometrial cancer, we further characterized this region and localized the hBRAG gene. The function of hBRAG has not yet been fully studied, and t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.7.6.1339
更新日期:2000-11-01 00:00:00
abstract::Tetraspanins are transmembrane adaptor proteins involved in the regulation of various fundamental cellular processes. For a number of malignant diseases, the level of expression of members of the tetraspanin family was found to correlate with tumor cell invasiveness, ability to form metastases, and poor clinical outco...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) hold great promise as potential therapies for tumors through the delivery of various anticancer agents. However, exogenous tissue‑derived MSCs, such as those of bone marrow, have exhibited a tendency for malignant transformation in the tumor microenvironment. This issue remains controvers...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7007
更新日期:2019-04-01 00:00:00
abstract::Metastasis is the main cause of death in osteosarcoma. Targeting the process of metastasis is a main strategy for osteosarcoma therapy. As a traditional Chinese medicine, Zanthoxylum nitidum (Roxb) has been applied to treat various diseases, including cancer. However, no evidence has been shown on the anti-metastasis ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4846
更新日期:2016-08-01 00:00:00
abstract::It has been recently reported that side population (SP) cells in nasopharyngeal carcinoma (NPC) cell lines display characteristics of cancer stem-like cells. However, the biological behavior and the significance of these cells for NPC progression remain unclear. In this study, we is...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1781
更新日期:2012-07-01 00:00:00
abstract::The promigratory molecule L1CAM is overexpressed in various tumors, often representing an unfavorable prognostic marker. Recently, we identified L1CAM expression in pancreatic ductal adenocarcinoma (PDAC) cells accounting for chemoresistance and increased cell migration. Thus, the present study aims at further elucida...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2010.909
更新日期:2010-10-01 00:00:00
abstract::Alterations in DNA methylation have a central role in the development and outcome of most human malignancies. Non‑small cell lung cancer (NSCLC), the most common lung cancer, leads to the largest number of cancer‑related deaths worldwide. FBJ murine osteosarcoma viral oncogene homolog B (FOSB) is a key component of th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7431
更新日期:2020-02-01 00:00:00
abstract::There has been increasing evidence that numerous bioactive dietary agents can hamper the process of carcinogenesis by targeting epigenetic alterations including DNA methylation. This therapeutic approach is considered as a significant goal for cancer therapy due to the reversible nature of epigenetic-mediated gene sil...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3802
更新日期:2015-04-01 00:00:00
abstract::The purpose of this study was to define gene expression profile changes in colorectal tumors in order to identify target genes involved in neoplastic progression. cDNA microarray analysis was used to detect differences in gene expression profiles between colon tumor samples obtained from 20 patients in different tumor...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::The S100A2 gene has been reported to be a putative tumor‑suppressor gene. Nevertheless, overexpression of S100A2 has been found in certain types of cancer. This study investigated S100A2 expression in tissue specimens of gastritis, intestinal metaplasia, adenomatous dysplasia and ga...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2236
更新日期:2013-04-01 00:00:00
abstract::Previous studies have demonstrated the tremendous tropism of mesenchymal stem cells (MSCs) for malignant gliomas, making these cells a potential vehicle for delivery of therapeutic genes to disseminated glioma cells. However, the mechanisms underlying the tropism of MSCs for gliomas remain poorly defined. It has been ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000796
更新日期:2010-06-01 00:00:00
abstract::Currently, various types of keratins have been reported to be highly expressed in cancer cells and to be associated with a malignant phenotype. In the present study, it was found that expression levels of keratin 6 (K6), keratin 16 (K16), and keratin 17 (K17) were highly elevated in SNU601 cells resistant to cisplatin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7201
更新日期:2019-08-01 00:00:00
abstract::Few studies on sarcomas have examined the relationships between microsatellite alterations in particular loci, tumor prognosis and tumorigenesis, because sarcomas are uncommon and those prognoses can be confounded by coexisting factors, such as tumor site. We studied the relationship between microsatellite alterations...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.4.917
更新日期:2001-07-01 00:00:00
abstract::Differentiation of breast cancer has been receiving increasing attention in attempts to minimize the numbers of excisional biopsies for benign tumors. Doppler spectral parameters, such as peak systolic velocity (PSV) and pulsatility index (PI), have been used but these are unable to differentiate breast cancers and be...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::Apoptin, a small protein derived from chicken anemia virus, possesses the capacity to specifically kill tumor cells while leaving normal cells intact. Previous studies have indicated that the subcellular localization of apoptin appears to be crucial for this tumor-selective activity. Apoptin resides in the cytoplasm o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2399
更新日期:2013-06-01 00:00:00
abstract::The present study aimed to examine the inhibitory effects of morusin on the human lung cancer cell line A549. Various doses of morusin were applied to A549 cells and the effects were assessed by wound‑healing and MTT assays, flow cytometry analysis of apoptosis, a mitochondrial membrane potential assay and RT‑PCR. The...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6431
更新日期:2018-07-01 00:00:00