Autophagy in human skin squamous cell carcinoma: Inhibition by 3-MA enhances the effect of 5-FU-induced chemotherapy sensitivity.

Abstract:

:Autophagy is an intracellular multi-step catabolic degradation process that involves the degradation and recycling of cellular proteins and cytoplasmic damaged organelles to maintain cellular homeostasis and reduction of metabolic stress. Numerous studies have indicated the importance of autophagy in cancer, but the role of autophagy in human skin squamous cell carcinoma (SSCC) development and response to therapy it is still unclear. In the present study, we investigated the role of autophagy in SSCC and the relationship with chemotherapy sensitivity. The present study demonstrated that autophagy related gene the microtubule-associated protein 1 light chain 3 (LC3) expression was low in SSCC. The negative correlation with Bcl2 and survivin, and the chemotherapy drug 5-FU increased the level of autophagy and the autophagy inhibitor 3-MA inhibited this effect in SSCC cells, time- and dose-dependently. When SSCC cells were treated first with 3-MA and then with 5-FU, the inhibition of proliferation, migration, invasion and apoptosis of SSCC cells was enhanced. Our results suggested the possibility of autophagy as a potential target in SSCC therapy and 3-MA and 5-FU combination treatment may be an effective SSCC therapy via autophagy modulating.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Zhang L,Zhang J,Chen L,Wang J

doi

10.3892/or.2015.4302

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

3147-55

issue

6

eissn

1021-335X

issn

1791-2431

journal_volume

34

pub_type

杂志文章
  • Inhibitor of DNA-binding protein 1 knockdown arrests the growth of colorectal cancer cells and suppresses hepatic metastasis in vivo.

    abstract::Inhibitor of DNA-binding protein 1 (ID1) is commonly abnormally overexpressed in colorectal cancer (CRC); yet, the functional significance of ID1 in the growth and invasive properties of CRC cells remains largely unclear. The present study investigated the effects of ID1 downregulation on the cell growth and metastati...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3172

    authors: Lai X,Liao J,Lin W,Huang C,Li J,Lin J,Chen Q,Ye Y

    更新日期:2014-07-01 00:00:00

  • TRIM28 knockdown increases sensitivity to etoposide by upregulating E2F1 in non-small cell lung cancer.

    abstract::Tripartite motif containing 28 (TRIM28) is a universal corepressor for Kruppel‑associated box zinc finger proteins. In our previous study, it was shown that expression of TRIM28 is upregulated in non‑small cell lung cancer (NSCLC) cell lines and tissues. Here, we demonstrated that the stable silencing of TRIM28 expres...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5638

    authors: Liu L,Xiao L,Liang X,Chen L,Cheng L,Zhang L,Wu X,Xu Q,Ma C

    更新日期:2017-06-01 00:00:00

  • Effects of miR‑181a on the biological function of multiple myeloma.

    abstract::The present study aimed to investigate the role of miR‑181a in multiple myeloma (MM) cell lines. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect the expression of microRNA (miR)‑181a. The MM cell line RPMI 8226 stably transduced with miR‑181a mimics or inhibitor was establishe...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7160

    authors: Liu N,Yang J,Yuan R,Peng J,Liu L,Guo X

    更新日期:2019-07-01 00:00:00

  • Hepatitis B virus X protein sensitizes HL-7702 cells to oxidative stress-induced apoptosis through modulation of the mitochondrial permeability transition pore.

    abstract::Chronic hepatitis B virus (HBV) infection is a leading cause of liver cirrhosis and cancer. Among the pathogenic factors of HBV, HBV X protein (HBx) is attracting increased attention. Although it is documented that HBx is a multifunctional regulator that modulates cell inflammation and apoptosis, the exact mechanism r...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.5225

    authors: Gao WY,Li D,Cai DE,Huang XY,Zheng BY,Huang YH,Chen ZX,Wang XZ

    更新日期:2017-01-01 00:00:00

  • Differential regulation of DEC2 among hypoxia-inducible genes in endometrial carcinomas.

    abstract::In this study, we demonstrate an important role of activation of the hypoxia-inducible factor-1 (HIF-1) pathway in endometrial carcinogenesis and tumor phenotype development of endometrial carcinoma, and suggest a unique role of the HIF-1-target gene, differentiated embryo chondrocyte 2 (DEC2), in carcinogenesis. Hypo...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yunokawa M,Tanimoto K,Nakamura H,Nagai N,Kudo Y,Kawamoto T,Kato Y,Hiyama E,Hiyama K,Nishiyama M

    更新日期:2007-04-01 00:00:00

  • Predictive factors in patients with advanced and metastatic squamous cell carcinoma of the head and neck: a study based on SWOG protocol S0420.

    abstract::To evaluate the prognostic values of different protein expression in the progression of squamous cell carcinoma of the head and neck (SCCHN) patients, we conducted immunohistochemical (IHC) analysis in tissue samples of different patients enrolled on SWOG protocol S0420. S0420 was a phase II trial to evaluate the effi...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2374

    authors: Mehta S,Moon J,Hashmi M,Leblanc M,Huang CH,Rinehart E,Wolf GT,Urba SG,Banerjee SK,Williamson S

    更新日期:2013-06-01 00:00:00

  • KIAA1199 and its biological role in human cancer and cancer cells (review).

    abstract::KIAA1199 is a gene included in the Human Unidentified Gene-Encoded (HUGE) large protein database which contains more than 2,400 members identified in the Kazusa cDNA sequencing project. Early studies described KIAA1199 as an inner ear-specific protein in which 3 point mutations were found to be associated with non‑syn...

    journal_title:Oncology reports

    pub_type: 杂志文章,评审

    doi:10.3892/or.2014.3038

    authors: Zhang Y,Jia S,Jiang WG

    更新日期:2014-04-01 00:00:00

  • Leukemia inhibitory factor receptor negatively regulates the metastasis of pancreatic cancer cells in vitro and in vivo.

    abstract::Pancreatic cancer (PC) is one of the leading causes of cancer-related deaths worldwide. Frequent metastasis and recurrence are the main reasons for the poor prognosis of PC patients. Thus, the discovery of new biomarkers and wider insights into the mechanisms involved in pancreatic tumorigenesis and metastasis is cruc...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4865

    authors: Ma D,Jing X,Shen B,Liu X,Cheng X,Wang B,Fu Z,Peng C,Qiu W

    更新日期:2016-08-01 00:00:00

  • Co-expression of herpes simplex virus thymidine kinase and Escherichia coli nitroreductase by an hTERT-driven adenovirus vector in breast cancer cells results in additive anti-tumor effects.

    abstract::Breast cancers especially in the late and metastatic stages remain refractory to treatment despite advances in surgical techniques and chemotherapy. Tumor-specific promoter-directed suicide gene therapy and adenoviral technology can be promising strategies for such advanced disease. Previous studies suggested that com...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1285

    authors: Yu B,Zhang Y,Zhan Y,Zha X,Wu Y,Zhang X,Dong Q,Kong W,Yu X

    更新日期:2011-07-01 00:00:00

  • Honeybee venom possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression on cervical cancer cell line.

    abstract::Bee venom (BV) therapy is a type of alternative medical treatment used to treat various diseases in oriental medicine. The mechanisms underlying the effects of BV remain poorly understood. In the present study, we evaluated the antiviral effect of BV on cervical carcinoma cell lines (CaSki, HeLa, C33A and TC-1). BV tr...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.3760

    authors: Kim YW,Chaturvedi PK,Chun SN,Lee YG,Ahn WS

    更新日期:2015-04-01 00:00:00

  • Active immunotherapy for mouse breast cancer with irradiated whole-cell vaccine expressing VEGFR2.

    abstract::As tumor-associated antigens are not well characterized for the majority of human tumors, polyvalent vaccines prepared with whole-tumor antigens are an attractive approach for tumor vaccination. Vascular endothelial growth factor receptor-2 (VEGFR2), as a model antigen with which to...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2282

    authors: Yan HX,Cheng P,Wei HY,Shen GB,Fu LX,Ni J,Wu Y,Wei YQ

    更新日期:2013-04-01 00:00:00

  • Diagnosis of hepatocellular carcinoma using digital subtraction imaging with the contrast agent, Levovist: comparison with helical CT, digital subtraction angiography, and US angiography.

    abstract::Digital subtraction imaging was compared to helical CT, DSA, and US angiography to assess its usefulness in the evaluation of vascularity in hepatocellular carcinoma (HCC). Digital subtraction imaging using Levovist as the contrast agent was performed in 41 patients with 43 nodules (22 men and 19 women, aged 50 to 83 ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yamamoto K,Shiraki K,Deguchi M,Sugimoto K,Sakai T,Ohmori S,Murata K,Hashimoto A,Shimizu A,Okuda Y,Nakano T

    更新日期:2002-07-01 00:00:00

  • Functional analysis of protein disulfide isomerase P5 in glioblastoma cells as a novel anticancer target.

    abstract::P5, which is a member of the protein disulfide isomerase family, possesses isomerase and chaperone activity in vitro; however, the physiological functions of this enzyme in cells remain unclear. To understand the important roles of P5 in cancer cells, the present study examined its expression on the surface of normal ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6868

    authors: Horibe T,Torisawa A,Masuda Y,Kawakami K

    更新日期:2019-02-01 00:00:00

  • Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene.

    abstract::The objective of the present study was to explore the in vitro and in vivo anticancer effects of Platycodin D (PD), derived from Platycodin grandiflorum, on highly metastatic MDA-MB-231 breast cancer cells. Using the MTT assay, we found that PD inhibited MDA-MB-231 cell growth in a concentration-dependent manner, with...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4935

    authors: Kong Y,Lu ZL,Wang JJ,Zhou R,Guo J,Liu J,Sun HL,Wang H,Song W,Yang J,Xu HX

    更新日期:2016-09-01 00:00:00

  • Predicting radiotherapy response for patients with soft tissue sarcoma by developing a molecular signature.

    abstract::Soft tissue sarcomas are rare and aggressive tumors arising from connective tissues. Adjuvant radiotherapy is a commonly used treatment approach for the majority of sarcomas. We attempted to identify a gene signature that can predict radiosensitive patients who are most likely to have a better treatment response from ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5999

    authors: Tang Z,Zeng Q,Li Y,Zhang X,Suto MJ,Xu B,Yi N

    更新日期:2017-11-01 00:00:00

  • Epimedium koreanum Nakai inhibits PMA-induced cancer cell migration and invasion by modulating NF-κB/MMP-9 signaling in monomorphic malignant human glioma cells.

    abstract::Previously, we showed that the herbal extract EYK (Epimedium koreanum Nakai) can regulate the immune response. Other studies showed that EYK has beneficial effects in human lung cancer, angiogenesis and Alzheimer's disease (AD). However, it remains unknown whether EYK can affect cancer cell migration and invasion in h...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.6043

    authors: Lee W,Nam JH,Cho HJ,Lee JY,Cho WK,Kim Υ,We YM,Ma JY,Hoe HS

    更新日期:2017-12-01 00:00:00

  • Expression of transglutaminases in human breast cancer and their possible clinical significance.

    abstract::Implicated in several physiologic processes, including tumour-host, some transglutaminases (TGase) have been noted to play a regulatory role in the extracellular matrix in cell adhesion and migration of cancer cells. This study sought to determine the level of expression of TGases and their possible clinical significa...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Jiang WG,Ablin R,Douglas-Jones A,Mansel RE

    更新日期:2003-11-01 00:00:00

  • Expression and activation of P38 MAP kinase in invasive ductal breast cancers: correlation with expression of the estrogen receptor, HER2 and downstream signaling phosphorylated proteins.

    abstract::MAP kinase signaling proteins have major implications in the molecular oncogenesis of breast cancers and have been extensively investigated as putative targets for therapy. This study reports the investigation of the expression of P38 MAPK and its phosphorylated form (p-P38 MAPK) in clinical specimens of invasive brea...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2645

    authors: Merlin JL,Harlé A,Lion M,Ramacci C,Leroux A

    更新日期:2013-10-01 00:00:00

  • Differential gene methylation patterns in cancerous and non‑cancerous cells.

    abstract::Large‑scale projects, such as The Cancer Genome Atlas (TCGA), Human Epigenome Project (HEP) and Human Epigenome Atlas (HEA), provide an insight into DNA methylation and histone modification markers. Changes in the epigenome significantly contribute to the initiation and progression of cancer. The goal of the present s...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7159

    authors: Kamińska K,Białkowska A,Kowalewski J,Huang S,Lewandowska MA

    更新日期:2019-07-01 00:00:00

  • Regulation of BGC-823 cell sensitivity to adriamycin via miRNA-135a-5p.

    abstract::MicroRNAs (miRNAs) play an important role in the genesis and development of gastric cancer. In the present study, we determined whether miRNA-135a-5p expression was increased in gastric cancer compared with adjacent non-tumor tissues using 20 pairs of gastric cancer and para-carcinoma tissue samples which were assesse...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3546

    authors: Pan Y,Ren F,Zhang W,Liu G,Yang D,Hu J,Feng K,Feng Y

    更新日期:2014-12-01 00:00:00

  • Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies.

    abstract::Single nucleotide polymorphisms (SNPs) in cancer-related genes can act as low risk genetic factors for the development of this disease. SNPs have also been shown to influence the efficacy and toxicity of various cytotoxic agents used in the treatment of cancer. Progress in these important areas of cancer research reli...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Grieu F,Joseph D,Norman P,Iacopetta B

    更新日期:2004-02-01 00:00:00

  • Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest in HL-60 cells.

    abstract::Diallyl disulfide (DADS) has shown potential as a therapeutic agent in various cancers. Previously, we found that myeloid cell leukemia sequence 1 (Mcl1) was downregulated in DADS-induced cell cycle arrest in HL-60 human leukemia cells. Here, we investigated the role of this protein...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1704

    authors: Yi L,Ji XX,Tan H,Feng MY,Tang Y,Wen L,Su Q

    更新日期:2012-06-01 00:00:00

  • Nras and Kras mutation in Japanese lung cancer patients: Genotyping analysis using LightCycler.

    abstract::Activating mutations of Ras gene families have been found in a variety of human malignancies, including lung cancer, suggesting their dominant role in tumorigenesis. Many studies have showed that the Kras gene is activated by point mutations in approximately 15-20% of non-small cell lung cancers (NSCLCs), however, the...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Sasaki H,Okuda K,Kawano O,Endo K,Yukiue H,Yokoyama T,Yano M,Fujii Y

    更新日期:2007-09-01 00:00:00

  • Hydrogen gas restores exhausted CD8+ T cells in patients with advanced colorectal cancer to improve prognosis.

    abstract::Exhausted cluster of differentiation (CD)8+ T cells lose immunological activity due to mitochondrial dysfunction caused by peroxisome proliferator‑activated receptor γ coactivator 1α (PGC‑1α) inactivation, resulting in a poor prognosis in patients with cancer. As hydrogen gas was recently reported to activate PGC‑1α, ...

    journal_title:Oncology reports

    pub_type: 杂志文章,随机对照试验

    doi:10.3892/or.2018.6841

    authors: Akagi J,Baba H

    更新日期:2019-01-01 00:00:00

  • Chemokines mediate mesenchymal stem cell migration toward gliomas in vitro.

    abstract::Previous studies have demonstrated the tremendous tropism of mesenchymal stem cells (MSCs) for malignant gliomas, making these cells a potential vehicle for delivery of therapeutic genes to disseminated glioma cells. However, the mechanisms underlying the tropism of MSCs for gliomas remain poorly defined. It has been ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00000796

    authors: Xu F,Shi J,Yu B,Ni W,Wu X,Gu Z

    更新日期:2010-06-01 00:00:00

  • Mina53 nuclear localization is an important indicator of prognosis in patients with colorectal cancer after adjuvant chemotherapy.

    abstract::The aim of this study was to investigate the status of the c‑Myc‑related molecule Mina53 and the clinical impact of Mina53 nuclear localization in patients with stage II and III colorectal cancer (CRC). Patients (n=250) who underwent complete resection of CRC at our department were enrolled in this study, and tissue m...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6407

    authors: Fujino S,Kinugasa T,Sudo T,Mizobe T,Yoshida T,Yoshida N,Ohchi T,Tajiri K,Yuge K,Nagasu S,Katagiri M,Akagi Y

    更新日期:2018-07-01 00:00:00

  • Structure, expression and mutational analysis of the hBRAG gene on 10q in the frequently deleted region in human endometrial cancer.

    abstract::We previously reported that chromosome arm 10q is one of the target regions of allelic loss in human endometrial cancer. To identify the gene in this region responsible for endometrial cancer, we further characterized this region and localized the hBRAG gene. The function of hBRAG has not yet been fully studied, and t...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.7.6.1339

    authors: Yuki M,Yoshinaga K,Yamakawa H,Sakurada K,Sato S,Yajima A,Horii A

    更新日期:2000-11-01 00:00:00

  • CRISPR/Cas9-mediated genome engineering of CXCR4 decreases the malignancy of hepatocellular carcinoma cells in vitro and in vivo.

    abstract::CXC chemokine receptor 4 (CXCR4) is associated with poor clinical outcomes and decreased survival in hepatocellular carcinoma (HCC). In the present study, we targeted CXCR4 by CRISPR/Cas9 in HepG2 cells and observed the effects both in vitro and in vivo. The results indicated that after targeting CXCR4 the expression ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5601

    authors: Wang X,Zhang W,Ding Y,Guo X,Yuan Y,Li D

    更新日期:2017-06-01 00:00:00

  • Upregulation of ABCG2 via the PI3K-Akt pathway contributes to acidic microenvironment-induced cisplatin resistance in A549 and LTEP-a-2 lung cancer cells.

    abstract::Hypoxia always exists in the processes involved in the development of lung cancer and contributes to an acidic microenvironment. Despite that hypoxia in the tumor microenvironment is associated with the formation of chemotherapeutic resistance, the exact role of an acidic microenvironment in the development of hypoxia...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4827

    authors: Hu CF,Huang YY,Wang YJ,Gao FG

    更新日期:2016-07-01 00:00:00

  • Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro.

    abstract::We investigated whether or not the mitochondrial genotypes affect radiation-induced micronucleus (MN) formation. For that purpose, the rho+, KT1 and rho0 human osteosarcoma cell lines were used, which carry the wild-type mitochondrial DNA (mtDNA), the tRNALys mutant mtDNA and no mtDNA, respectively. Despite no signifi...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.8.3.615

    authors: Yoshida K,Yamazaki H,Ozeki S,Inoue T,Yoshioka Y,Yoneda M,Fujiwara Y,Inoue T

    更新日期:2001-05-01 00:00:00